Biochemical and molecular characterization of the diphenol oxidase of Cryptococcus neoformans: identification as a laccase.

abstract：:Bacteriophage T7 RNA polymerase is stable in Escherichia coli but very susceptible to cleavage by at least one endoprotease after cell lysis. The major source of this endoprotease activity was found to be localized to the outer membrane of the cell. A rapid whole-cell assay was developed to screen different strains fo...

journal_title：Journal of bacteriology

authors： Grodberg J,Dunn JJ

更新日期：1988-03-01 00:00:00

abstract：:Xylanase activity of Clostridium cellulovorans, an anaerobic, mesophilic, cellulolytic bacterium, was characterized. Most of the activity was secreted into the growth medium when the bacterium was grown on xylan. Furthermore, when the extracellular material was separated into cellulosomal and noncellulosomal fractions...

journal_title：Journal of bacteriology

authors： Kosugi A,Murashima K,Doi RH

更新日期：2001-12-01 00:00:00

abstract：:The nucleotide sequence of the DNA encoding the ADP-ribosyltransferase (A1) fragment of cholera enterotoxin was determined. A putative precursor of the A1 peptide contains an 18-amino acid leader peptide, and the mature A1 peptide contains 194 amino acids. The primary structure of the A1 fragment from cholera enteroto...

journal_title：Journal of bacteriology

authors： Lockman HA,Galen JE,Kaper JB

更新日期：1984-09-01 00:00:00

abstract：:A class of galactose-resistant mutants has been derived from strains of Salmonella typhimurium which are defective in uridine diphosphoglucose-4-epimerase. Resistant strains are phenotypically similar to parent organisms but do not lyse in the presence of galactose. Low levels of functional epimerase can be detected i...

journal_title：Journal of bacteriology

authors： Shuster CW,Rundell K

更新日期：1969-10-01 00:00:00

abstract：:The 5 to 10 peritrichously inserted complex flagella of Rhizobium meliloti MVII-1 were found to form right-handed flagellar bundles. Bacteria swam at speeds up to 60 microns/s, their random three-dimensional walk consisting of straight runs and quick directional changes (turns) without the vigorous angular motion (tum...

journal_title：Journal of bacteriology

authors： Götz R,Schmitt R

更新日期：1987-07-01 00:00:00

abstract：:Acetosyringone, a phenolic inducer of the virulence (vir) genes of Agrobacterium tumefaciens, inhibited the growth of the nopaline-type strains T37 and C58 incubated under acidic conditions. In the course of a 6-day incubation with acetosyringone, avirulent clones were produced in different proportions by strains T37 ...

journal_title：Journal of bacteriology

authors： Fortin C,Nester EW,Dion P

更新日期：1992-09-01 00:00:00

abstract：:The c subunit of Streptococcus mutans ATP synthase (FoF1) is functionally exchangeable with that of Escherichia coli, since E. coli with a hybrid FoF1 is able to grow on minimum succinate medium through oxidative phosphorylation. E. coli F1 bound to the hybrid Fo with the S. mutans c subunit showed N,N'-dicyclohexylca...

journal_title：Journal of bacteriology

authors： Araki M,Hoshi K,Fujiwara M,Sasaki Y,Yonezawa H,Senpuku H,Iwamoto-Kihara A,Maeda M

更新日期：2013-11-01 00:00:00

abstract：:An expressed sequence tag homologous to cheA was previously isolated by random sequencing of Thermotoga maritima cDNA clones (C. W. Kim, P. Markiewicz, J. J. Lee, C. F. Schierle, and J. H. Miller, J. Mol. Biol. 231: 960-981, 1993). Oligonucleotides complementary to this sequence tag were synthesized and used to identi...

journal_title：Journal of bacteriology

authors： Swanson RV,Sanna MG,Simon MI

更新日期：1996-01-01 00:00:00

abstract：:A 22-kilobase fragment of the Escherichia coli chromosome which contains the genes for translation initiation factor 3, phenylalanyl-tRNA synthetase, and threonyl-tRNA synthetase was cloned into plasmid pACYC184. The hybrid plasmid (designated pID1) complements a temperature-sensitive pheS lesion in E. coli NP37. pID1...

journal_title：Journal of bacteriology

authors： Elseviers D,Gallagher P,Hoffman A,Weinberg B,Schwartz I

更新日期：1982-10-01 00:00:00

abstract：:The WO-1 strain of Candida albicans is capable of alternating between two highly distinct yeast cell types termed white and opaque (E. H. A. Rikkerrink, B. B. Magee, and P. T. Magee, J. Bacteriol. 170:895-899, 1988; B. Slutsky, M. Staebell, J. Anderson, L. Risen, M. Pfaller, and D. R. Soll, J. Bacteriol. 169:189-197, ...

journal_title：Journal of bacteriology

authors： McEachern MJ,Hicks JB

更新日期：1991-12-01 00:00:00

abstract：:The region of the Rhizobium leguminosarum biovar viciae Sym plasmid pRL1JI, responsible for the production and secretion of a previously described 50-kilodalton protein (R. A. de Maagd, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg, J. Bacteriol. 170:4424-4427, 1988), was cloned and its nucleotide sequence was de...

journal_title：Journal of bacteriology

authors： de Maagd RA,Wijfjes AH,Spaink HP,Ruiz-Sainz JE,Wijffelman CA,Okker RJ,Lugtenberg BJ

更新日期：1989-12-01 00:00:00

abstract：:A number of independently derived uracil-requiring mutant strains of the parent strain 168 were mapped by inter-mutant transformation crosses. Only a few of these mutant strains were found to be transformable. Studies were performed in which these transformable uracil-requiring mutant strains were used as the recipien...

journal_title：Journal of bacteriology

authors： Kelleher RJ Jr,Gooder H

更新日期：1973-11-01 00:00:00

abstract：:The Staphylococcus aureus autoinducer-2 (AI-2) producer protein LuxS is phosphorylated by the Ser/Thr kinase Stk1 at a unique position, Thr14. The enzymatic activity of the phosphorylated isoform of LuxS was abrogated compared to that of nonphosphorylated LuxS, thus providing the first evidence of an AI-2-producing en...

journal_title：Journal of bacteriology

authors： Cluzel ME,Zanella-Cléon I,Cozzone AJ,Fütterer K,Duclos B,Molle V

更新日期：2010-12-01 00:00:00

abstract：:Methanomonas methanooxidans incorporates both carbon atoms of acetate into the glutamate and aspartate families of amino acids during growth on methane; carbon dioxide is also evolved from both carbon atoms of acetate. The distribution of carboxyl-labeled acetate incorporated into convalently bound glutamate is consis...

journal_title：Journal of bacteriology

authors： Wadzinski AM,Ribbons DW

更新日期：1975-07-01 00:00:00

abstract：:Upon cold shock, Escherichia coli cell growth transiently stops. During this acclimation phase, specific cold shock proteins (CSPs) are highly induced. At the end of the acclimation phase, their synthesis is reduced to new basal levels, while the non-cold shock protein synthesis is resumed, resulting in cell growth re...

journal_title：Journal of bacteriology

authors： Yamanaka K,Inouye M

更新日期：2001-05-01 00:00:00

abstract：:Escherichia coli prefers growth in neutral pH environments but can withstand extremely acidic conditions (pH 2) for long periods. Of the four E. coli systems that contribute to acid resistance, one, the glutamate-dependent system, is remarkable in its efficacy and regulatory complexity. The resistance mechanism involv...

journal_title：Journal of bacteriology

authors： Ma Z,Masuda N,Foster JW

更新日期：2004-11-01 00:00:00

abstract：:The opportunistic pathogen Pseudomonas aeruginosa produces multifunctional, polar, filamentous appendages termed type IV pili. Type IV pili are involved in colonization during infection, twitching motility, biofilm formation, bacteriophage infection, and natural transformation. Electrostatic surface analysis of modele...

journal_title：Journal of bacteriology

authors： van Schaik EJ,Giltner CL,Audette GF,Keizer DW,Bautista DL,Slupsky CM,Sykes BD,Irvin RT

更新日期：2005-02-01 00:00:00

abstract：:RpoS is a conserved alternative sigma factor that regulates the expression of many stress response genes in Escherichia coli. The RpoS regulon is large but has not yet been completely characterized. In this study, we report the identification of over 100 RpoS-dependent fusions in a genetic screen based on the differen...

journal_title：Journal of bacteriology

authors： Vijayakumar SR,Kirchhof MG,Patten CL,Schellhorn HE

更新日期：2004-12-01 00:00:00

abstract：:When ratios of the major polypeptides of the outer membrane isolated from cells of Escherichia coli B grown in minimal medium containing either a single amino acid or several amino acids were compared, no difference was observed. However, the ratio of these polypeptides in outer membrane material released into the med...

journal_title：Journal of bacteriology

authors： Loeb MR,Kilner J

更新日期：1979-02-01 00:00:00

abstract：:In Salmonella typhimurium the two enzymes of proline catabolism, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid dehydrogenase, are subject to catabolite repression when the cells are grown in the presence of glucose. Mutants partially relieved of catabolite repression (PutR) for the proline catabolic enzymes...

journal_title：Journal of bacteriology

authors： Newell SL,Brill WJ

更新日期：1972-08-01 00:00:00

abstract：:A beta-1,4-xylan hydrolase (xylanase A) produced by Erwinia chrysanthemi D1 isolated from corn was analyzed with respect to its secondary structure and enzymatic function. The pH and temperature optima for the enzyme were found to be pH 6.0 and 35 degrees C, with a secondary structure under those conditions that consi...

journal_title：Journal of bacteriology

authors： Hurlbert JC,Preston JF 3rd

更新日期：2001-03-01 00:00:00

abstract：:A selection procedure is described which permits a large number of Saccharomyces cerevisiae mutants to be screened for specific lesions in mitochondrial respiratory enzymes and the adenosine triphosphatase. The method has been used to isolate nuclear mutant strains with specific lesions in coenzyme QH2-cytochrome c re...

journal_title：Journal of bacteriology

authors： Tzagoloff A,Akai A,Needleman RB

更新日期：1975-06-01 00:00:00

abstract：:The strict human pathogen Neisseria gonorrhoeae is exposed to oxidative damage during infection. N. gonorrhoeae has many defenses that have been demonstrated to counteract oxidative damage. However, recN is the only DNA repair and recombination gene upregulated in response to hydrogen peroxide (H(2)O(2)) by microarray...

journal_title：Journal of bacteriology

authors： Stohl EA,Seifert HS

更新日期：2006-11-01 00:00:00

abstract：:Activation of the late prespore-specific RNA polymerase sigma factor sigma(G) during Bacillus subtilis sporulation coincides with completion of the engulfment process, when the prespore becomes a protoplast fully surrounded by the mother cell cytoplasm and separated from it by a double membrane system. Activation of s...

journal_title：Journal of bacteriology

authors： Serrano M,Vieira F,Moran CP Jr,Henriques AO

更新日期：2008-12-01 00:00:00

abstract：:HlyD, a member of the membrane fusion protein family, is essential for the secretion of the RTX hemolytic toxin HlyA from Escherichia coli. Random point mutations affecting HlyA secretion were obtained, distributed in most periplasmic regions of the HlyD molecule. Analysis of the secretion phenotypes of different muta...

journal_title：Journal of bacteriology

authors： Pimenta AL,Racher K,Jamieson L,Blight MA,Holland IB

更新日期：2005-11-01 00:00:00

abstract：:The peptidoglycan cell wall determines the shape and structural integrity of a bacterial cell. Class B penicillin-binding proteins (PBPs) carry a transpeptidase activity that cross-links peptidoglycan strands via their peptide side chains, and some of these proteins are directly involved in cell shape determination. N...

journal_title：Journal of bacteriology

authors： Wei Y,Havasy T,McPherson DC,Popham DL

更新日期：2003-08-01 00:00:00

abstract：:The antibiotic albomycin is highly effective against Streptococcus pneumoniae, with an MIC of 10 ng/ml. The reason for the high efficacy was studied by measuring the uptake of albomycin into S. pneumoniae. Albomycin was transported via the system that transports the ferric hydroxamates ferrichrome and ferrioxamine B. ...

journal_title：Journal of bacteriology

authors： Pramanik A,Braun V

更新日期：2006-06-01 00:00:00

abstract：:The Gram-positive bacterium Streptococcus pyogenes (also called group A Streptococcus [GAS]), is found strictly in humans and is capable of causing a wide variety of infections. Here we demonstrate that serine catabolism in GAS is controlled by the transcriptional regulator Spy49_0126c. We have designated this regulat...

journal_title：Journal of bacteriology

authors： LaSarre B,Federle MJ

更新日期：2011-04-01 00:00:00

abstract：:The biological activities of Bacillus subtilis ribosomes pretreated with 0.5, 1, or 2 mmp-chloromercuribenzoate (PCMB) were examined. The ribosomes treated with 1 or 2 mm PCMB lost their capacity to synthesize polyphenylalanine as well as the capacity to bind phenylalanyl-transfer ribonucleic acid. On the other hand, ...

journal_title：Journal of bacteriology

authors： Ranu RS,Kaji A

更新日期：1972-10-01 00:00:00

abstract：:We have isolated a hybrid plasmid, pDB(mei2)2, containing a 7.4-kilobases (kb) DNA fragment from a Schizosaccharomyces pombe genomic library which is able to complement the mei2 mutation of S. pombe. Integration of the cloned DNA sequence at the mei2 site on chromosome I demonstrated that it contained the mei2 gene. T...

journal_title：Journal of bacteriology

authors： Shimoda C,Uehira M,Kishida M,Fujioka H,Iino Y,Watanabe Y,Yamamoto M

更新日期：1987-01-01 00:00:00