DNA binding: a novel function of Pseudomonas aeruginosa type IV pili.

abstract：:The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor (rafR gene) to raf operator (rafO) DNA. Both rafR and rafO have been sequenced. The 1,011-base-pair rafR gene encodes a 336-amino-acid polypeptide containing an N-terminal helix-turn-helix motif. rafO, as defined by in vivo ti...

journal_title：Journal of bacteriology

authors： Aslanidis C,Schmitt R

更新日期：1990-04-01 00:00:00

abstract：:Lysates of Escherichia coli Ymel obtained from cultures grown in the absence of tryptophan in minimal medium supplemented with 0.1% casein hydrolysate show an approximate fivefold increase in steady-state specific activity of both anthranilate synthetase and tryptophan synthetase A protein relative to cultures grown i...

journal_title：Journal of bacteriology

authors： Stubbs JD,Stubbs EA

更新日期：1971-12-01 00:00:00

abstract：:Myxococcus xanthus dsp and dif mutants have similar phenotypes in that they are deficient in social motility and fruiting body development. We compared the two loci by genetic mapping, complementation with a cosmid clone, DNA sequencing, and gene disruption and found that 16 of the 18 dsp alleles map to the dif genes....

journal_title：Journal of bacteriology

authors： Lancero H,Brofft JE,Downard J,Birren BW,Nusbaum C,Naylor J,Shi W,Shimkets LJ

更新日期：2002-03-01 00:00:00

abstract：:High-density tiling microarray and RNA sequencing technologies were used to analyze the transcriptome of the periodontopathogenic bacterium Porphyromonas gingivalis. The compiled P. gingivalis transcriptome profiles were based on total RNA samples isolated from three different laboratory culturing conditions, and the ...

journal_title：Journal of bacteriology

authors： Høvik H,Yu WH,Olsen I,Chen T

更新日期：2012-01-01 00:00:00

abstract：:Evolution by natural selection occurs in cultures of Escherichia coli maintained under carbon starvation stress. Mutants of increased fitness express a growth advantage in stationary phase (GASP) phenotype, enabling them to grow and displace the parent as the majority population. The first GASP mutation was identified...

journal_title：Journal of bacteriology

authors： Zinser ER,Kolter R

更新日期：2000-08-01 00:00:00

abstract：:Enzymes of the ATP-independent Deg serine endopeptidase family are very flexible with regard to their substrate specificity. Some family members cleave only one substrate, while others act as general proteases on unfolded substrates. The proteolytic activity of Deg proteases is regulated by PDZ protein interaction dom...

journal_title：Journal of bacteriology

authors： Huesgen PF,Scholz P,Adamska I

更新日期：2007-09-01 00:00:00

abstract：:Bacillus subtilis mutants with reduced rates of cell wall autolysis reached a constant rate of wall turnover after a longer lag than the standard strain but eventually showed the same turnover rate. In reverse, a turnover-deficient mutant autolysed at a slightly higher rate than the standard strain. Consequently, ther...

journal_title：Journal of bacteriology

authors： Vitković L,Cheung HY,Freese E

更新日期：1984-01-01 00:00:00

abstract：:We report the cloning and characterization of the biosynthetic gene cluster (crtE, crtB, crtI, crtE2, crtYg, crtYh, and crtX) of the γ-cyclic C(50) carotenoid sarcinaxanthin in Micrococcus luteus NCTC2665. Expression of the complete and partial gene cluster in Escherichia coli hosts revealed that sarcinaxanthin biosyn...

journal_title：Journal of bacteriology

authors： Netzer R,Stafsnes MH,Andreassen T,Goksøyr A,Bruheim P,Brautaset T

更新日期：2010-11-01 00:00:00

abstract：:Osmotic stress was found to induce biofilm formation in a Staphylococcus aureus mucosal isolate. Inactivation of a global regulator of the bacterial stress response, the alternative transcription factor sigma(B), resulted in a biofilm-negative phenotype and loss of salt-induced biofilm production. Complementation of t...

journal_title：Journal of bacteriology

authors： Rachid S,Ohlsen K,Wallner U,Hacker J,Hecker M,Ziebuhr W

更新日期：2000-12-01 00:00:00

abstract：:sigma E is a sporulation-specific sigma factor of Bacillus subtilis that is formed from an inactive precursor protein (pro-sigma E) by the removal of 27 to 29 amino acids from the pro-sigma E amino terminus. By using oligonucleotide-directed mutagenesis, sequential deletions were constructed in the precursor-specific ...

journal_title：Journal of bacteriology

authors： Peters HK 3rd,Carlson HC,Haldenwang WG

更新日期：1992-07-01 00:00:00

abstract：:Two distinct spore appendage types of Clostridium bifermentans, a pinlike appendage and a tubular appendage, were studied by electron microscopy. The pinlike appendage is characterized by a shaft, about 100 A in diameter, which has a lobed caplike structure. The tubular appendage, 500 to 600 A in diameter, is characte...

journal_title：Journal of bacteriology

authors： Yolton DP,Pope L,Williams MG,Rode LJ

更新日期：1968-01-01 00:00:00

abstract：:Eklund, Curtis (The University of Texas, Austin) and Orville Wyss. Enzyme associated with bacteriophage infection. J. Bacteriol. 84:1209-1215. 1962.-A capsule-digesting enzyme was formed when azotobacter cells were infected with bacteriophage. The enzyme appeared in the medium when the phages lysed the cells. By disru...

journal_title：Journal of bacteriology

authors： Eklund C,Wyss O

更新日期：1962-12-01 00:00:00

abstract：:Bacillus subtilis 168ts-200B is a temperature-sensitive mutant of B. subtilis 168 which grows as rods at 30 C but as irregular spheres at 45 C. Growth at the nonpermissive temperature resulted in a deficiency of teichoic acid in the cell wall. A decrease in teichoic acid synthesis coupled with the rapid turnover of th...

journal_title：Journal of bacteriology

authors： Boylan RJ,Mendelson NH,Brooks D,Young FE

更新日期：1972-04-01 00:00:00

abstract：:Little is known about the effect of the host on the genetic stability of bacterial plant pathogens. Crown gall, a plant disease caused by Agrobacterium tumefaciens, may represent a useful model to study this effect. Indeed, our previous observations on the natural occurrence and origin of nonpathogenic agrobacteria su...

journal_title：Journal of bacteriology

authors： Bélanger C,Canfield ML,Moore LW,Dion P

更新日期：1995-07-01 00:00:00

abstract：:The sheath of the filamentous, gliding cyanobacterium Phormidium uncinatum was studied by using light and electron microscopy. In thin sections and freeze fractures the sheath was found to be composed of helically arranged carbohydrate fibrils, 4 to 7 nm in diameter, which showed a substantial degree of crystallinity....

journal_title：Journal of bacteriology

authors： Hoiczyk E

更新日期：1998-08-01 00:00:00

abstract：:The uptake of homologous DNA by Haemophilus influenzae was studied as a function of the proton motive force in completely competent cultures in the pH range of 6 to 8. The composition and magnitude of the proton motive force were varied by using the ionophores valinomycin and nigericin (in the presence of various pota...

journal_title：Journal of bacteriology

authors： Bremer W,Kooistra J,Hellingwerf KJ,Konings WN

更新日期：1984-03-01 00:00:00

abstract：:The ski22::Tn917lac insertion mutation in Bacillus subtilis was isolated in a screen for mutations that cause a defect in sporulation but are suppressed by the presence or overexpression of the histidine protein kinase encoded by kinA (spoIIJ). The ski22::Tn917lac insertion mutation was in ald, the gene encoding alani...

journal_title：Journal of bacteriology

authors： Siranosian KJ,Ireton K,Grossman AD

更新日期：1993-11-01 00:00:00

abstract：:The Escherichia coli HtrA protein is a periplasmic protease/chaperone that is upregulated under stress conditions. The protease and chaperone activities of HtrA eliminate or refold damaged and unfolded proteins in the bacterial periplasm that are generated upon stress conditions. In the absence of substrates, HtrA oli...

journal_title：Journal of bacteriology

authors： Jomaa A,Iwanczyk J,Tran J,Ortega J

更新日期：2009-03-01 00:00:00

abstract：:A 3,373-base-pair DNA segment from a clone fortuitously isolated from Saccharopolyspora erythraea by hybridization to an oligodeoxynucleotide probe was sequenced. Computer-assisted analysis of the nucleotide sequence reveals three closely linked Streptomyces open reading frames plus a fourth converging on the others. ...

journal_title：Journal of bacteriology

authors： Donadio S,Shafiee A,Hutchinson CR

更新日期：1990-01-01 00:00:00

abstract：:Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy) mutants. The degU32(Hy) mutation caused a 99% reduction in the expression of a sigD-lacZ translational fusion gene cons...

journal_title：Journal of bacteriology

authors： Tokunaga T,Rashid MH,Kuroda A,Sekiguchi J

更新日期：1994-08-01 00:00:00

abstract：:Bacterial cells sense the extracellular environment and adapt to that environment by activating gene regulation circuits, often by means of signaling molecules. The Serratia marcescens hemophore is a signaling molecule that acts as an extracellular heme-scavenging protein. The heme-loaded hemophore interacts with its ...

journal_title：Journal of bacteriology

authors： Cwerman H,Wandersman C,Biville F

更新日期：2006-05-01 00:00:00

abstract：:Extracellular muramidase-2 of Enterococcus hirae ATCC 9790 was purified to homogeneity by substrate binding, guanidine-HCl extraction, and reversed-phase chromatography. A monoclonal antibody, 2F8, which specifically recognizes muramidase-2, was used to screen a genomic library of E. hirae ATCC 9790 DNA in bacteriopha...

journal_title：Journal of bacteriology

authors： Chu CP,Kariyama R,Daneo-Moore L,Shockman GD

更新日期：1992-03-01 00:00:00

abstract：:The protein motive force of metabolizing Bacillus subtilis cells was only slightly affected by changes in the external pH between 5 and 8, although the electrical component and the chemical component of the proton motive force contributed differently at different external pH. The electrical component of the proton mot...

journal_title：Journal of bacteriology

authors： Shioi JI,Matsuura S,Imae Y

更新日期：1980-12-01 00:00:00

abstract：:The Bacillus subtilis ureABC operon encodes homologs of the three subunits of urease enzymes of the family Enterobacteriaceae. Disruption of ureC prevented utilization of urea as a nitrogen source and resulted in a partial growth defect in minimal medium containing limiting amounts of arginine or allantoin as the sole...

journal_title：Journal of bacteriology

authors： Cruz-Ramos H,Glaser P,Wray LV Jr,Fisher SH

更新日期：1997-05-01 00:00:00

abstract：:The human bacterial pathogens Chlamydia spp. possess a genus-specific lipopolysaccharide as a major surface antigen, the structure of which has been determined by analytical chemistry as Kdop alpha 2-8-Kdop alpha 2-4-Kdop alpha 2-6GlcNp beta 1-6-GlcNol (Kdo, 3-deoxy-D-manno-2-octulosonic acid). Immunochemical studies ...

journal_title：Journal of bacteriology

authors： Holst O,Brade L,Kosma P,Brade H

更新日期：1991-03-01 00:00:00

abstract：:Smith, Paul F. (University of Pennsylvania, Philadelphia) and George H. Rothblat. Comparison of pleuropneumonia-like and L-type organisms. J. Bacteriol. 83:500-506. 1962.-The content of total lipid and nonsaponifiable lipid depended upon the type of organism; the greatest amounts were found in pleuropneumonia-like org...

journal_title：Journal of bacteriology

authors： SMITH PF,ROTHBLAT GH

更新日期：1962-03-01 00:00:00

abstract：:The biochemical consequences of inositol limitation in an inositol auxotroph of Neurospora crassa have been examined as a means of disclosing the cellular role of inositol. The cellular levels of inositol in the inl mutant were proportional to the concentration of inositol in the growth medium whereas inositol phospha...

journal_title：Journal of bacteriology

authors： Hanson BA

更新日期：1980-07-01 00:00:00

abstract：:Direct evidence for the involvement of ubiquinone in H2 oxidation by Rhizobium japonicum was demonstrated; H2 reduced ca. 80% of the extractable ubiquinone. The inhibitor 2-n-heptyl-4-hydroxyquinoline-N-oxide blocked electron transport at a site between ubiquinone and the cytochromes. The results showed that no cytoch...

journal_title：Journal of bacteriology

authors： O'Brian MR,Maier RJ

更新日期：1985-02-01 00:00:00

abstract：:A systematic survey for the presence of plasmids in 17 different xenobiotic-degrading Sphingomonas strains was performed. In almost all analyzed strains, two to five plasmids with sizes of about 50 to 500 kb were detected by using pulsed-field gel electrophoresis. A comparison of plasmid preparations untreated or trea...

journal_title：Journal of bacteriology

authors： Basta T,Keck A,Klein J,Stolz A

更新日期：2004-06-01 00:00:00

abstract：:A new method has been developed in order to select mutants showing decreased puc operon transcription in Rhodobacter sphaeroides 2.4.1. A transcriptional fusion of a promoterless fragment derived from the sacB gene, encoding the levansucrase from Bacillus subtilis, to the upstream regulatory region of the puc operon h...

journal_title：Journal of bacteriology

authors： Sabaty M,Kaplan S

更新日期：1996-01-01 00:00:00