Role of the electrochemical proton gradient in genetic transformation of Haemophilus influenzae.

abstract：:pdrK encodes a pyridoxine (PN)/pyridoxal (PL)/pyridoxamine (PM) kinase thought to function in the salvage pathway of pyridoxal 5'-phosphate (PLP) coenzyme biosynthesis. The observation that pdxK null mutants still contain PL kinase activity led to the hypothesis that Escherichia coli K-12 contains at least one other B...

journal_title：Journal of bacteriology

authors： Yang Y,Tsui HC,Man TK,Winkler ME

更新日期：1998-04-01 00:00:00

abstract：:We report that transformation of Streptomyces lividans with cloned DNA of the SLP1 genetic element results in integration of the element at the same chromosomal locus (attB) normally occupied by SLP1 in its original host, Streptomyces coelicolor, and in S. lividans that has received SLP1 by mating. We constructed SLP1...

journal_title：Journal of bacteriology

authors： Omer CA,Stein D,Cohen SN

更新日期：1988-05-01 00:00:00

abstract：:Cell suspensions of Bacteroides fragilis were allowed to ferment glucose and lactate labeled with (14)C in different positions. The fermentation products, propionate and acetate, were isolated, and the distribution of radioactivity was determined. An analysis of key enzymes of possible pathways was also made. The resu...

journal_title：Journal of bacteriology

authors： Macy JM,Ljungdahl LG,Gottschalk G

更新日期：1978-04-01 00:00:00

abstract：:Recently, a new type of K+ transporter, Ktr, has been identified in the bacterium Vibrio alginolyticus (T. Nakamura, R. Yuda, T. Unemoto, and E. P. Bakker, J. Bacteriol. 180:3491-3494, 1998). The Ktr transport system consists of KtrB, an integral membrane subunit, and KtrA, a subunit peripherally bound to the cytoplas...

journal_title：Journal of bacteriology

authors： Holtmann G,Bakker EP,Uozumi N,Bremer E

更新日期：2003-02-01 00:00:00

abstract：:SpoVM is an unusually small (26-residue-long) protein that is produced in the mother cell chamber of the sporangium during the process of sporulation in Bacillus subtilis. We investigated the subcellular localization of SpoVM, which is believed to be an amphipathic alpha-helix, by using a fusion of the sporulation pro...

journal_title：Journal of bacteriology

authors： van Ooij C,Losick R

更新日期：2003-02-01 00:00:00

abstract：:Species of marine bacteria belonging to the genus Beneckea and strains of Photobacterium fischeri were negatively stained and examined by means of the electron microscope to determine the structure and arrangement of their flagella. All of the species of the genus Beneckea had single, polar, sheathed flagella when gro...

journal_title：Journal of bacteriology

authors： Allen RD,Baumann P

更新日期：1971-07-01 00:00:00

abstract：:The kinetics of respiration-dependent proton efflux and membrane energization have been studied in intact cells of logarithmic-phase Escherichia coli. Parallel measurements of the rate and extent of proton efflux into the external medium (half-time, about 10 s; ratio of H(+) to O, about 0.5) and the oxidation of E. co...

journal_title：Journal of bacteriology

authors： Gould JM

更新日期：1979-04-01 00:00:00

abstract：:In the chloroplasts of higher plants and algae, the biosynthesis of the chlorophyll precursor delta-aminolevulinic acid (ALA) involves at least three enzymes and a tRNA species. Here we demonstrate that in cell extracts of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 ALA was formed from glutamate i...

journal_title：Journal of bacteriology

authors： O'Neill GP,Peterson DM,Schön A,Chen MW,Söll D

更新日期：1988-09-01 00:00:00

abstract：:The EcfG-type sigma factor RpoE2 is the regulator of the general stress response in Sinorhizobium meliloti. RpoE2 activity is negatively regulated by two NepR-type anti-sigma factors (RsiA1/A2), themselves under the control of two anti-anti-sigma factors (RsiB1/B2) belonging to the PhyR family of response regulators. ...

journal_title：Journal of bacteriology

authors： Sauviac L,Bruand C

更新日期：2014-07-01 00:00:00

abstract：:The effects of various metabolic inhibitors on the motility of Spirochaeta aurantia were investigated. After 15 min in sodium arsenate buffer, 90% of cells remained motile even though adenosine triphosphate levels dropped from 5.6 to 0.1 nmol/mg (dry weight) of cells. After 70 min in sodium arsenate, 5% of cells were ...

journal_title：Journal of bacteriology

authors： Goulbourne EA Jr,Greenberg EP

更新日期：1980-09-01 00:00:00

abstract：:Expression of the Escherichia coli OmpC and OmpF outer membrane proteins is regulated by the osmolarity of the culture media. In contrast, expression of OmpC in Salmonella typhi is not influenced by osmolarity, while OmpF is regulated as in E. coli. To better understand the lack of osmoregulation of OmpC expression in...

journal_title：Journal of bacteriology

authors： Martínez-Flores I,Cano R,Bustamante VH,Calva E,Puente JL

更新日期：1999-01-01 00:00:00

abstract：:Transducing frequencies of phage P22 lysates prepared from Salmonella typhimurium exponential cultures in minimal and nutrient broth media were compared. The assumption is that cells grown in a minimal medium will have one replication fork per replication unit, but cells in nutrient broth will have multiple replicatio...

journal_title：Journal of bacteriology

authors： Fujisawa T,Eisenstark A

更新日期：1973-07-01 00:00:00

abstract：:Escherichia coli strain TP985, which has an insertional mutation in the gene for the periplasmic murein tripeptide binding protein MppA, was previously reported to overproduce MarA and exhibit a multiple-antibiotic resistance (Mar) phenotype (H. Li and J. T. Park, J. Bacteriol. 181:4842-4847, 1999). We found that TP98...

journal_title：Journal of bacteriology

authors： Bina X,Perreten V,Levy SB

更新日期：2003-02-01 00:00:00

abstract：:DNA sequence analysis revealed that the putative yhdJ DNA methyltransferase gene of Escherichia coli is 55% identical to the Nostoc sp. strain PCC7120 gene encoding DNA methyltransferase AvaIII, which methylates adenine in the recognition sequence, ATGCAT. The yhdJ gene was cloned, and the enzyme was overexpressed and...

journal_title：Journal of bacteriology

authors： Kossykh VG,Lloyd RS

更新日期：2004-04-01 00:00:00

abstract：:Vibrio vulnificus is a human pathogen that produces lethal septicemia in susceptible persons, and the primary virulence factor for this organism is capsular polysaccharide (CPS). The role of the capsule in V. vulnificus biofilms was examined under a variety of conditions, by using either defined CPS mutants or spontan...

journal_title：Journal of bacteriology

authors： Joseph LA,Wright AC

更新日期：2004-02-01 00:00:00

abstract：:SufA, IscA, and Nfu have been proposed to function as scaffolds in the assembly of Fe/S clusters in bacteria. To investigate the roles of these proteins further, single and double null-mutant strains of Synechococcus sp. strain PCC 7002 were constructed by insertional inactivation of genes homologous to sufA, iscA, an...

journal_title：Journal of bacteriology

authors： Balasubramanian R,Shen G,Bryant DA,Golbeck JH

更新日期：2006-05-01 00:00:00

abstract：:Accumulation of intracellular allantoin and allantoate is mediated by two distinct active transport systems in Saccharomyces cerevisiae. Allantoin transport (DAL4 gene) is inducible, while allantoate uptake is constitutive (it occurs at full levels in the absence of any allantoate-related compounds from the culture me...

journal_title：Journal of bacteriology

authors： Chisholm VT,Lea HZ,Rai R,Cooper TG

更新日期：1987-04-01 00:00:00

abstract：:Cystic fibrosis (CF) patients are highly susceptible to chronic pulmonary disease caused by mucoid Pseudomonas aeruginosa strains that overproduce the exopolysaccharide alginate. We showed here that a mutation in zwf, encoding glucose-6-phosphate dehydrogenase (G6PDH), leads to a approximately 90% reduction in alginat...

journal_title：Journal of bacteriology

authors： Silo-Suh L,Suh SJ,Phibbs PV,Ohman DE

更新日期：2005-11-01 00:00:00

abstract：:DifA is a methyl-accepting chemotaxis protein (MCP)-like sensory transducer that regulates exopolysaccharide (EPS) production in Myxococcus xanthus. Here mutational analysis and molecular biology were used to probe the signaling mechanisms of DifA in EPS regulation. We first identified the start codon of DifA experime...

journal_title：Journal of bacteriology

authors： Xu Q,Black WP,Nascimi HM,Yang Z

更新日期：2011-02-01 00:00:00

abstract：:The transduction efficiency of Tn551, a staphylococcal transposon coding for erythromycin resistance (ermB), was increased by a factor of about five in acceptor strains carrying the gene for methicillin resistance (mec), as compared with methicillin-sensitive strains. This enhancement was independent of the generalize...

journal_title：Journal of bacteriology

authors： Berger-Bächi B

更新日期：1983-04-01 00:00:00

abstract：:We describe the identification and characterization of two genes and their gene products responsible for determining receptor binding and pilus length in type 1-piliated Escherichia coli. One gene, pilE, conferred the ability of piliated cells to agglutinate guinea pig erythrocytes. The other gene, pilF, determined pi...

journal_title：Journal of bacteriology

authors： Maurer L,Orndorff PE

更新日期：1987-02-01 00:00:00

abstract：:DNA uptake by natural competence is a central process underlying the genetic plasticity, biology, and virulence of the human respiratory opportunistic pathogen Streptococcus pneumoniae A study reported in this issue (J. Slager, R. Aprianto, and J.-W. Veening, J. Bacteriol. 201:e00780-18, https://doi.org/10.1128/JB.007...

journal_title：Journal of bacteriology

authors： Winkler ME,Morrison DA

更新日期：2019-06-10 00:00:00

abstract：:The region of plasmid NR1 concerned with resistance to Hg2+ and organomercurials consists of sequences found on restriction endonuclease fragments EcoRI-H and EcoRI-I. When both fragments were cloned together into a derivative of plasmid ColE1, the hybrid plasmid conferred properties indistinguishable from those of th...

journal_title：Journal of bacteriology

authors： Nakahara H,Silver S,Miki T,Rownd RH

更新日期：1979-10-01 00:00:00

abstract：:Mycoplasma penetrans is a newly identified species of the genus MYCOPLASMA: It was first isolated from a urine sample from a human immunodeficiency virus (HIV)-infected patient. M. penetrans changes its surface antigen profile with high frequency. The changes originate from ON<==>OFF phase variations of the P35 family...

journal_title：Journal of bacteriology

authors： Horino A,Sasaki Y,Sasaki T,Kenri T

更新日期：2003-01-01 00:00:00

abstract：:Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.) and Guy P. Youmans. Effect of mycosuppressin on the respiration and growth of Mycobacterium tuberculosis. J. Bacteriol. 84:708-715. 1962.-A substance, called mycosuppressin, was found in the lungs of guinea pigs and rabbits vaccinated with BCG or...

journal_title：Journal of bacteriology

authors： YOUMANS AS,YOUMANS GP

更新日期：1962-10-01 00:00:00

abstract：:A nonswarming Serratia liquefaciens mutant deficient in serrawettin W2 production was constructed by transposon mutagenesis. Sequence homology indicated that insertion had occurred in gene swrA, which encodes a putative peptide synthetase. Expression of swrA is controlled by quorum sensing. ...

journal_title：Journal of bacteriology

authors： Lindum PW,Anthoni U,Christophersen C,Eberl L,Molin S,Givskov M

更新日期：1998-12-01 00:00:00

abstract：:Reich, Paul R. (National Institutes of Health, Bethesda, Md.), Norman L. Somerson, James A. Rose, and Sherman M. Weissman. Genetic relatedness among mycoplasmas as determined by nucleic acid homology. J. Bacteriol. 91:153-160. 1966.-A sensitive membrane filter method to detect nucleic acid homology was used to determi...

journal_title：Journal of bacteriology

authors： Reich PR,Somerson NL,Rose JA,Weissman SM

更新日期：1966-01-01 00:00:00

abstract：:If bacteria are incapable of forming and incorporating proteins into the cytoplasmic membranes in all phases of the cell cycle, then not all cells from an asynchronous culture should be capable of growth when switched to a new carbon and energy source whose metabolism requires new membrane function. The transfer of an...

journal_title：Journal of bacteriology

authors： Koch AL

更新日期：1975-10-01 00:00:00

abstract：:The secondary structures of the eubacterial RNase P RNAs are being elucidated by a phylogenetic comparative approach. Sequences of genes encoding RNase P RNA from each of the recognized subgroups (alpha, beta, gamma, and delta) of the proteobacteria have now been determined. These sequences allow the refinement, to ne...

journal_title：Journal of bacteriology

authors： Brown JW,Haas ES,James BD,Hunt DA,Liu JS,Pace NR

更新日期：1991-06-01 00:00:00

abstract：:Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA and ChiB (ArChiA and ArChiB), in response to chitin induction. A single chromosomal DNA fragment containing the genes c...

journal_title：Journal of bacteriology

authors： Lonhienne T,Mavromatis K,Vorgias CE,Buchon L,Gerday C,Bouriotis V

更新日期：2001-03-01 00:00:00