Mapping of Myxococcus xanthus social motility dsp mutations to the dif genes.

abstract：:In this report we describe the functional expression of EmrE, a 110-amino-acid multidrug transporter from Escherichia coli, in the yeast Saccharomyces cerevisiae. To allow for phenotypic complementation, a mutant strain sensitive to a series of cationic lipophilic drugs was first identified. A hemagglutinin epitope-ta...

journal_title：Journal of bacteriology

authors： Yelin R,Rotem D,Schuldiner S

更新日期：1999-02-01 00:00:00

abstract：:A novel method is described that allows a direct determination of the location of a portion of the dipicolinic acid (DPA) in spores. Ultraviolet (UV) irradiation is shown to cause cross-linkage of DPA to spore proteins which have the characteristics of membrane proteins. We suggest that DPA resides in the inner foresp...

journal_title：Journal of bacteriology

authors： Germaine GR,Murrell WG

更新日期：1974-04-01 00:00:00

abstract：:The KRE1 gene of Saccharomyces cerevisiae, sacKRE1, appears to be involved in the synthesis of cell wall beta-glucan. S. cerevisiae strains with mutations in the KRE1 gene produce a structurally altered cell wall (1----6)-beta-glucan, which results in resistance to K1 killer toxin. We isolated the canKRE1 gene from Ca...

journal_title：Journal of bacteriology

authors： Boone C,Sdicu A,Laroche M,Bussey H

更新日期：1991-11-01 00:00:00

abstract：:We have evaluated the use of [1,2-13C2]propionate for the analysis of propionic acid metabolism, based on the ability to distinguish between the methylcitrate and methylmalonate pathways. Studies using propionate-adapted Escherichia coli MG1655 cells were performed. Preservation of the 13C-13C-12C carbon skeleton in l...

journal_title：Journal of bacteriology

authors： London RE,Allen DL,Gabel SA,DeRose EF

更新日期：1999-06-01 00:00:00

abstract：:We have investigated a tyrosine kinase activity from Pseudomonas solanacearum, an economically important plant pathogen. In vitro incubation of membrane fractions with [gamma-32P]ATP and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed an 85-kDa phosphoprotein. Phosphorylation of this prot...

journal_title：Journal of bacteriology

authors： Atkinson M,Allen C,Sequeira L

更新日期：1992-07-01 00:00:00

abstract：:Pili isolated from Pseudomonas aeruginosa PAK were solubilized in the detergent octyl-glucoside. Subsequent removal of the detergent by dialysis resulted in the formation of short rods of about 9 nm in diameter and various lengths. The aggregation process was followed by ultracentrifugation, viscometry, and electron m...

journal_title：Journal of bacteriology

authors： Watts TH,Scraba DG,Paranchych W

更新日期：1982-09-01 00:00:00

abstract：:Bacteriophage phi29 protein p6 is a viral architectural protein, which binds along the whole linear phi29 DNA in vivo and is involved in initiation of DNA replication and transcription control. Protein p1 is a membrane-associated viral protein, proposed to attach the viral genome to the cell membrane. Protein p17 is i...

journal_title：Journal of bacteriology

authors： González-Huici V,Alcorlo M,Salas M,Hermoso JM

更新日期：2004-12-01 00:00:00

abstract：:A gene for the constitutive ribosephosphate isomerase (rpiA) is highly cotransducible with serA at 56.2 min on the genetic linkage map of Escherichia coli K-12. Suppression of ribosephosphate isomerase A-negative mutants can occur by a regulator gene mutation permitting constitutive synthesis of the normally inducible...

journal_title：Journal of bacteriology

authors： Skinner AJ,Cooper RA

更新日期：1974-06-01 00:00:00

abstract：:Three bacteriophages were tested for ability to transduce the plasmid of pPL10 between W mutant derivatives of Bacillus pumilus NRS 576. Phage PBP1- and PMB1-generated plasmid transductants occurred at about 10% the frequency of transductants for a chromosome marker. Phage PBS1-generated plasmid transductants occurred...

journal_title：Journal of bacteriology

authors： Bramucci MG,Lovett PS

更新日期：1976-08-01 00:00:00

abstract：:Spectinomycin-resistant (Spcr) mutants of Escherichia coli were isolated from nutrient agar plates containing 20% sucrose and 100 mug of spectinomycin per ml. About one-third of the Spcr mutants thus obtained were sucrose dependent (Sucd) and were classified into two types: I, those unable to grow on sucrose-free medi...

journal_title：Journal of bacteriology

authors： Miyoshi Y,Yamagata H

更新日期：1976-01-01 00:00:00

abstract：:RpoS, the sigma factor of enteric bacteria that responds to stress and stationary phase, is subject to complex regulation acting at multiple levels, including transcription, translation, and proteolysis. Increased translation of rpoS mRNA during growth at low temperature, after osmotic challenge, or with a constitutiv...

journal_title：Journal of bacteriology

authors： Jones AM,Goodwill AG,Elliott T

更新日期：2006-07-01 00:00:00

abstract：:Escherichia coli K-12 strain CU1008 cannot use L-serine as the sole carbon source, but it could use L-serine as an auxiliary carbon source with glucose, L-alanine, or pyruvate and could derive energy from L-serine to support oxygen uptake. CU1008 grew with L-serine if it was also provided with glycine and leucine. The...

journal_title：Journal of bacteriology

authors： Newman EB,Walker C

更新日期：1982-08-01 00:00:00

abstract：:The nucleotide sequence of the DNA mobilization region of the 5-nitroimidazole resistance plasmid pIP421, from strain BF-F239 of Bacteroides fragilis, was determined. It contains a putative origin of transfer (oriT) including three sets of inverted repeats and two sequences reminiscent of specific integration host fac...

journal_title：Journal of bacteriology

authors： Trinh S,Reysset G

更新日期：1997-06-01 00:00:00

abstract：:Deletions in the rpoBC region have been transferred to phage lambda and characterized in detail by genetic, structural, and functional tests. We thus extend and confirm knowledge of the organization of this part of the chromosome. The new phages are useful tools for studying the genes for the bacterial transcription a...

journal_title：Journal of bacteriology

authors： Linn T,Goman M,Scaife J

更新日期：1979-11-01 00:00:00

abstract：:It previously has been shown that lkyD mutants of Salmonella typhimurium form large blebs of outer membrane over the septal and polar regions of dividing cells. To determine whether the outer membrane blebs are formed over potential sites of division even in the absence of septal ingrowth, lkyD strains were studied un...

journal_title：Journal of bacteriology

authors： Fung JC,MacAlister TJ,Weigand RA,Rothfield LI

更新日期：1980-08-01 00:00:00

abstract：:Lambda transducing phage gamma 16-2 carries the genes envA, ftsZ, ftsA, ddl, and murC and directs the synthesis of six unique proteins in ultraviolet-irradiated cells. Various derivatives of gamma 16-2 carrying smaller segments of the bacterial deoxyribonucleic acid have also been analyzed for their capacity to direct...

journal_title：Journal of bacteriology

authors： Lutkenhaus JF,Wu HC

更新日期：1980-09-01 00:00:00

abstract：:Smith, Paul F. (University of Pennsylvania, Philadelphia) and George H. Rothblat. Comparison of pleuropneumonia-like and L-type organisms. J. Bacteriol. 83:500-506. 1962.-The content of total lipid and nonsaponifiable lipid depended upon the type of organism; the greatest amounts were found in pleuropneumonia-like org...

journal_title：Journal of bacteriology

authors： SMITH PF,ROTHBLAT GH

更新日期：1962-03-01 00:00:00

abstract：:Polyglutamate is found in various bacteria, but displays different functions depending on the species and their environment. Here, we describe a minimal polyglutamate synthesis system in Bacillus anthracis. In addition to the three genes previously described as sufficient for polyglutamate synthesis, this system inclu...

journal_title：Journal of bacteriology

authors： Candela T,Mock M,Fouet A

更新日期：2005-11-01 00:00:00

abstract：:Protein and ribonucleic acid (RNA) syntheses were measured in both lethally injured and thermally damaged viable cells after heating at lethal temperatures. Immediately after heating, cells were incubated in growth media containing either [14C]leucine or [3H]uracil. The labeled cells were subsequently treated with pen...

journal_title：Journal of bacteriology

authors： Dean RG,McGroarty EJ

更新日期：1979-05-01 00:00:00

abstract：:Nisin is a cationic antimicrobial peptide that belongs to the group of lantibiotics. It is thought to form oligomeric pores in the target membrane by a mechanism that requires the transmembrane electrical potential delta psi and that involves local pertubation of the lipid bilayer structure. Here we show that nisin do...

journal_title：Journal of bacteriology

authors： Moll GN,Clark J,Chan WC,Bycroft BW,Roberts GC,Konings WN,Driessen AJ

更新日期：1997-01-01 00:00:00

abstract：:Previously, we identified a gene (aldA) from Myxococcus xanthus, which we suggested encoded the enzyme alanine dehydrogenase on the basis of similarity to known Ald protein sequences (M. J. Ward, H. Lew, A. Treuner-Lange, and D. R. Zusman, J. Bacteriol. 180:5668-5675, 1998). In this study, we have confirmed that aldA ...

journal_title：Journal of bacteriology

authors： Ward MJ,Lew H,Zusman DR

更新日期：2000-01-01 00:00:00

abstract：:Amplification of the mraY gene, previously called open reading frame Y (ORF-Y, 1,080 bp), at 2 min in the chromosome map of Escherichia coli enhanced the activity of UDP-N-acetylmuramoyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase (EC 2.7.8.13). This enzyme catalyzes the form...

journal_title：Journal of bacteriology

authors： Ikeda M,Wachi M,Jung HK,Ishino F,Matsuhashi M

更新日期：1991-02-01 00:00:00

abstract：:Crude cell extracts of Bacillus subtilis 168T exhibit enzyme activity capable of releasing free uracil from phage PBS1 deoxyribonucleic acid (DNA) in the presence of ethylenediaminetetraacetate. By measuring the enzyme activity in 300 clones that emanated from mutagenized cells, we obtained a mutant strain that did no...

journal_title：Journal of bacteriology

authors：

更新日期：1977-08-01 00:00:00

abstract：:Expression levels of Bacillus amyloliquefaciens alpha-amylase, Escherichia coli TEM-beta-lactamase, and Semliki Forest virus glycoprotein E1 genes were compared in Bacillus subtilis. All three model genes were expressed by using a secretion vector, constructed by joining the B. amyloliquefaciens alpha-amylase promoter...

journal_title：Journal of bacteriology

authors： Ulmanen I,Lundström K,Lehtovaara P,Sarvas M,Ruohonen M,Palva I

更新日期：1985-04-01 00:00:00

abstract：:Escherichia coli produces polysaccharide capsules that, based on their mechanisms of synthesis and assembly, have been classified into four groups. The group 4 capsule (G4C) polysaccharide is frequently identical to that of the cognate lipopolysaccharide O side chain and has, therefore, also been termed the O-antigen ...

journal_title：Journal of bacteriology

authors： Peleg A,Shifrin Y,Ilan O,Nadler-Yona C,Nov S,Koby S,Baruch K,Altuvia S,Elgrably-Weiss M,Abe CM,Knutton S,Saper MA,Rosenshine I

更新日期：2005-08-01 00:00:00

abstract：:Enteroaggregative Escherichia coli (EAggEC) has been associated with persistent pediatric diarrhea in the developing world, yet the pathogenetic mechanisms of EAggEC infection are unknown. Our previous data have suggested that aggregative adherence of some EAggEC strains to HEp-2 cells is mediated by flexible, bundle-...

journal_title：Journal of bacteriology

authors： Nataro JP,Yikang D,Yingkang D,Walker K

更新日期：1994-08-01 00:00:00

abstract：:The ethanol oxidase respiratory chain of Gluconobacter suboxydan was characterized by using G. suboxydans subsp. alpha, a variant species of G. suboxydans incapable of oxidizing ethanol. The membranes of G. suboxydans subsp. alpha exhibited neither alcohol dehydrogenase, ethanol oxidase, nor glucose-ferricyanide oxido...

journal_title：Journal of bacteriology

authors： Matsushita K,Nagatani Y,Shinagawa E,Adachi O,Ameyama M

更新日期：1991-06-01 00:00:00

abstract：:Electron microscopy of thin sections of dormant and germinating spores of Bacillus subtilis 168 revealed a progressive change in the structure of the cortex, outer spore coat, and inner spore coat. The initial changes were observed in the cortex region, which showed a loose fibrous network within 10 min of germination...

journal_title：Journal of bacteriology

authors： Santo LY,Doi RH

更新日期：1974-10-01 00:00:00

abstract：:Lactobacillus helveticus R0052 is a commercially available strain that is widely used in probiotic preparations. The genome sequence consisted of 2,129,425 bases. Comparative analysis showed that it was unique among L. helveticus strains in that it contained genes encoding mucus-binding proteins similar to those found...

journal_title：Journal of bacteriology

authors： Tompkins TA,Barreau G,Broadbent JR

更新日期：2012-11-01 00:00:00

abstract：:Thiobacillus neapolitanus, when grown in continuous culture with thiosulfate limiting growth, possessed an apparent maximal molar growth yield of 8.0 g (dry weight) per mole of thiosulfate. The substrate requirement for energy of maintenance was the highest yet reported, amounting to 21.8 mmoles of thiosulfate per g p...

journal_title：Journal of bacteriology

authors： Hempfling WP,Vishniac W

更新日期：1967-03-01 00:00:00