Isolation and characterization of a Bacillus subtilis mutant with a defective N-glycosidase activity for uracil-containing deoxyribonucleic acid.

abstract：:The enzyme system capable of converting coproporphyrinogen to protoporphyrin was demonstrated in the soluble fraction of extracts of Pseudomonas fluorescens grown aerobically, of P. denitrificans grown anaerobically under denitrifying conditions, and of Escherichia coli grown both aerobically and anaerobically. Protop...

journal_title：Journal of bacteriology

authors： Jacobs NJ,Jacobs JM,Brent P

更新日期：1970-05-01 00:00:00

abstract：:Evident in its name, the gastric pathogen Helicobacter pylori has a helical cell morphology which facilitates efficient colonization of the human stomach. An improved light-focusing strategy allowed us to robustly distinguish even subtle perturbations of H. pylori cell morphology by deviations in light-scattering prop...

journal_title：Journal of bacteriology

authors： Yang DC,Blair KM,Taylor JA,Petersen TW,Sessler T,Tull CM,Leverich CK,Collar AL,Wyckoff TJ,Biboy J,Vollmer W,Salama NR

更新日期：2019-06-21 00:00:00

abstract：:Median longitudinal sections of germ tube apices of Mucor rouxii revealed the presence of a single, roughly hemispherical, electron-dense organelle, in intimate contact with the apical cell wall. Conceivably, this "apical corpuscle" may be responsible for the emission of the germ tube and its continued apical growth. ...

journal_title：Journal of bacteriology

authors： Bartnicki-Garcia S,Nelson N,Cota-Robles E

更新日期：1968-06-01 00:00:00

abstract：:"Roseburia inulinivorans" is an anaerobic polysaccharide-utilizing firmicute bacterium from the human colon that was identified as a producer of butyric acid during growth on glucose, starch, or inulin. R. inulinivorans A2-194 is also able to grow on the host-derived sugar fucose, following a lag period, producing pro...

journal_title：Journal of bacteriology

authors： Scott KP,Martin JC,Campbell G,Mayer CD,Flint HJ

更新日期：2006-06-01 00:00:00

abstract：:The synthesis of pyruvate carboxylase (PC) was studied by using quantitative immunoblot analysis with an antibody raised against PC purified from Rhodobacter capsulatus and was found to vary 20-fold depending on the growth conditions. The PC content was high in cells grown on pyruvate or on carbon substrates metaboliz...

journal_title：Journal of bacteriology

authors： Yakunin AF,Hallenbeck PC

更新日期：1997-03-01 00:00:00

abstract：:There are significant differences in the large subunits of form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase isolated from Rhodopseudomonas sphaeroides. Two-dimensional peptide mapping of carboxymethylated large subunits clearly indicates that there are differences in the primary structure of the two ...

journal_title：Journal of bacteriology

authors： Gibson JL,Tabita FR

更新日期：1985-12-01 00:00:00

abstract：:Of 20 clinical isolates of Haemophilus parainfluenzae, 13 produced a mannose-resistant hemagglutinin that agglutinated erythrocytes from chickens, horses, rabbits, and sheep. Examination with the electron microscope showed that only strain HR-885 was pilate. Grown in static liquid culture, the 12 hemagglutinating, non...

journal_title：Journal of bacteriology

authors： Kahn ME,Gromkova R

更新日期：1981-02-01 00:00:00

abstract：:The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express higher levels of antibioti...

journal_title：Journal of bacteriology

authors： Seifert HS,Ajioka RS,Paruchuri D,Heffron F,So M

更新日期：1990-01-01 00:00:00

abstract：:We previously demonstrated that mutation of the staphylococcal accessory regulator (sarA) in a clinical isolate of Staphylococcus aureus (UAMS-1) results in an impaired capacity to form a biofilm in vitro (K. E. Beenken, J. S. Blevins, and M. S. Smeltzer, Infect. Immun. 71:4206-4211, 2003). In this report, we used a m...

journal_title：Journal of bacteriology

authors： Beenken KE,Dunman PM,McAleese F,Macapagal D,Murphy E,Projan SJ,Blevins JS,Smeltzer MS

更新日期：2004-07-01 00:00:00

abstract：:Lundgren, D. G. (Syracuse University, Syracuse, N.Y.) and J. J. Cooney. Chemical analyses of asporogenic mutants of Bacillus cereus. J. Bacteriol. 83:1287-1293. 1962.-Bacillus cereus ATCC 4342 and three temperature-sensitive asporogenic mutants were compared regarding some basic cellular components and response to met...

journal_title：Journal of bacteriology

authors： LUNDGREN DG,COONEY JJ

更新日期：1962-06-01 00:00:00

abstract：:In Escherichia coli K-12 mutants which had a new nalidixic acid resistance mutation at about 82 min on the chromosome map, cell growth was resistant to or hypersusceptible to nalidixic acid, oxolinic acid, piromidic acid, pipemidic acid, and novobiocin. Deoxyribonucleic acid gyrase activity as tested by supercoiling o...

journal_title：Journal of bacteriology

authors： Yamagishi J,Furutani Y,Inoue S,Ohue T,Nakamura S,Shimizu M

更新日期：1981-11-01 00:00:00

abstract：:Sinorhizobium meliloti, the nitrogen-fixing symbiont of alfalfa, has the ability to catabolize myo-, scyllo-, and D-chiro-inositol. Functional inositol catabolism (iol) genes are required for growth on these inositol isomers, and they play a role during plant-bacterium interactions. The inositol catabolism genes compr...

journal_title：Journal of bacteriology

authors： Kohler PR,Choong EL,Rossbach S

更新日期：2011-10-01 00:00:00

abstract：:The Dps-like peroxide resistance protein (Dpr) is essential for H2O2 stress tolerance and aerobic growth of the oral pathogen Streptococcus mutans Dpr accumulates during oxidative stress, protecting the cell by sequestering iron ions and thereby preventing the generation of toxic hydroxyl radicals that result from the...

journal_title：Journal of bacteriology

authors： Ganguly T,Kajfasz JK,Miller JH,Rabinowitz E,Galvão LCC,Rosalen PL,Abranches J,Lemos JA

更新日期：2018-06-25 00:00:00

abstract：:Romano, Antonio H. (University of Cincinnati, Cincinnati, Ohio), and Dorothy J. Geason. Pattern of sheath synthesis in Sphaerotilus natans. J. Bacteriol. 88:1145-1150. 1964.-Formation of the characteristic sheath of Sphaerotilus natans was followed by immunofluorescence. Fluorescent antisheath antibody was obtained by...

journal_title：Journal of bacteriology

authors： ROMANO AH,GEASON DJ

更新日期：1964-10-01 00:00:00

abstract：:Bacterial cell division is a complex process that relies on a multiprotein complex composed of a core of widely conserved and generally essential proteins and on accessory proteins that vary in number and identity in different bacteria. The assembly of this complex and, particularly, the initiation of constriction are...

journal_title：Journal of bacteriology

authors： Osorio A,Camarena L,Cevallos MA,Poggio S

更新日期：2017-03-28 00:00:00

abstract：:A modified transformation procedure that is effective for the introduction of plasmid deoxyribonucleic acid at high frequency into Salmonella typhimurium, as well as into Escherichia coli, is described. Transformed bacteria acquire a circular deoxyribonucleic acid species having the genetic and molecular characteristi...

journal_title：Journal of bacteriology

authors： Lederberg EM,Cohen SN

更新日期：1974-09-01 00:00:00

abstract：:Clostridium perfringens type B and D isolates produce epsilon-toxin, the third most potent clostridial toxin. The epsilon-toxin gene (etx) is plasmid borne in type D isolates, but etx genetics have been poorly studied in type B isolates. This study reports the first sequencing of any etx plasmid, i.e., pCP8533etx, fro...

journal_title：Journal of bacteriology

authors： Miyamoto K,Li J,Sayeed S,Akimoto S,McClane BA

更新日期：2008-11-01 00:00:00

abstract：:Acinetobacter baumannii has emerged as a significant nosocomial pathogen worldwide. The increasing trend of carbapenem and fluoroquinolone resistance in A. baumannii severely limits the usage of therapeutic antimicrobial agents. Here we report the genome sequence of a multidrug-resistant A. baumannii strain, TCDC-AB07...

journal_title：Journal of bacteriology

authors： Chen CC,Lin YC,Sheng WH,Chen YC,Chang SC,Hsia KC,Liao MH,Li SY

更新日期：2011-05-01 00:00:00

abstract：:A sequenced collection of plasmid-borne random fusions of Escherichia coli DNA to a Photorhabdus luminescens luxCDABE reporter was used as a starting point to select a set of 689 nonredundant functional gene fusions. This group, called LuxArray 1.0, represented 27% of the predicted transcriptional units in E. coli. Hi...

journal_title：Journal of bacteriology

authors： Van Dyk TK,DeRose EJ,Gonye GE

更新日期：2001-10-01 00:00:00

abstract：:Lysozyme Chalaropsis, a beta-1,4-N,6-O-diacetylmuramidase, attacks the cell wall murein of Staphylococcus aureus H in a random manner. In hypertonic sucrose solutions, true protoplasts are produced and, in the process, mesosomal tubules are extruded into the medium. ...

journal_title：Journal of bacteriology

authors： Robinson JP,Robinson RD,Hash JH

更新日期：1974-02-01 00:00:00

abstract：:We demonstrate that the expression of hem genes in Rhodobacter capsulatus is transcriptionally repressed in response to the exogenous addition of heme. A high-copy suppressor screen for regulators of hem gene expression resulted in the identification of an LysR-type transcriptional regulator, called HbrL, that regulat...

journal_title：Journal of bacteriology

authors： Smart JL,Bauer CE

更新日期：2006-02-01 00:00:00

abstract：:We report here the identification of a new lipoprotein, NlpI, in Escherichia coli K-12. The NlpI structural gene (nlpI) is located between the genes pnp (polynucleotide phosphorylase) and deaD (RNA helicase) at 71 min on the E. coli chromosome. The nlpI gene encodes a putative polypeptide of approximately 34 kDa, and ...

journal_title：Journal of bacteriology

authors： Ohara M,Wu HC,Sankaran K,Rick PD

更新日期：1999-07-01 00:00:00

abstract：:Tn5 was used to generate mutants that were deficient in the dissimilatory reduction of nitrite for Pseudomonas sp. strain G-179, which contains a copper nitrite reductase. Three types of mutants were isolated. The first type showed a lack of growth on nitrate, nitrite, and nitrous oxide. The second type grew on nitrat...

journal_title：Journal of bacteriology

authors： Ye RW,Averill BA,Tiedje JM

更新日期：1992-10-01 00:00:00

abstract：:The process of flagellar assembly in Salmonella typhimurium was investigated by using temperature-sensitive mutants. The mutants were grown at the restrictive temperature and then at the permissive temperature, with radiolabel supplied in the first phase of the experiment and not the second, or vice versa. Flagellar h...

journal_title：Journal of bacteriology

authors： Jones CJ,Macnab RM

更新日期：1990-03-01 00:00:00

abstract：:The ferric enterobactin (FeEnt) receptor CfrA is present in the majority of Campylobacter jejuni isolates and is responsible for high-affinity iron acquisition. Our recent work and that of others strongly suggested the existence of another FeEnt uptake system in Campylobacter. Here we have identified and characterized...

journal_title：Journal of bacteriology

authors： Xu F,Zeng X,Haigh RD,Ketley JM,Lin J

更新日期：2010-09-01 00:00:00

abstract：:Virulence genes in Listeria monocytogenes are coordinately expressed under the control of the transcriptional activator PrfA, encoded by prfA, a member of the cyclic AMP (cAMP) receptor protein (CRP)/FNR family of bacterial regulators. Strain P14-A is a spontaneous mutant of L. monocytogenes serovar 4b which produces ...

journal_title：Journal of bacteriology

authors： Ripio MT,Domínguez-Bernal G,Lara M,Suárez M,Vazquez-Boland JA

更新日期：1997-03-01 00:00:00

abstract：:Mutants of Escherichia coli K-12 unable to excise pyrimidine dimers from their deoxyribonucleic acid (DNA) because of a uvr mutation show a higher survival when plated on a minimal salts medium after exposure to ultraviolet radiation than when plated on a complex medium such as nutrient agar containing yeast extract. ...

journal_title：Journal of bacteriology

authors： Ganesan AK,Smith KC

更新日期：1970-05-01 00:00:00

abstract：:The chiR gene of Serratia marcescens 2170, encoding a LysR-type transcriptional activator, was identified previously as an essential factor for expression of chitinases and a chitin-binding protein, CBP21. To identify other genes that are essential for chitinase production, transposon mutagenesis with mini-Tn5Km1 was ...

journal_title：Journal of bacteriology

authors： Uchiyama T,Kaneko R,Yamaguchi J,Inoue A,Yanagida T,Nikaidou N,Regue M,Watanabe T

更新日期：2003-03-01 00:00:00

abstract：:Comparative genome analysis of Enterococcus faecium recently revealed that a genomic island containing the esp gene, referred to as the esp-containing pathogenicity island (esp PAI), can be transferred by conjugation and contains a partial Tn916-like element and an integrase gene, intA. Here, we characterize the role ...

journal_title：Journal of bacteriology

authors： Top J,Sinnige JC,Majoor EA,Bonten MJ,Willems RJ,van Schaik W

更新日期：2011-02-01 00:00:00

abstract：:Orf20 of the conjugative transposon Tn916 was purified as a chimeric protein fused to maltose binding protein (MBP-Orf20). The chimeric protein possessed endonucleolytic activity, cleaving both strands of the Tn916 origin of conjugal transfer (oriT) at several distinct sites and favoring GT dinucleotides. Incubation o...

journal_title：Journal of bacteriology

authors： Rocco JM,Churchward G

更新日期：2006-03-01 00:00:00