New nalidixic acid resistance mutations related to deoxyribonucleic acid gyrase activity.

Abstract:

:In Escherichia coli K-12 mutants which had a new nalidixic acid resistance mutation at about 82 min on the chromosome map, cell growth was resistant to or hypersusceptible to nalidixic acid, oxolinic acid, piromidic acid, pipemidic acid, and novobiocin. Deoxyribonucleic acid gyrase activity as tested by supercoiling of lambda phage deoxyribonucleic acid inside the mutants was similarly resistant or hypersusceptible to the compounds. The drug concentrations required for gyrase inhibition were much higher than those for cell growth inhibition but similar to those for inhibition of lambda phage multiplication. Transduction analysis with lambda phages carrying the chromosomal fragment of the tnaA-gyrB region suggested that one of the mutations, nal-31, was located on the gyrB gene.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Yamagishi J,Furutani Y,Inoue S,Ohue T,Nakamura S,Shimizu M

doi

10.1128/JB.148.2.450-458.1981

subject

Has Abstract

pub_date

1981-11-01 00:00:00

pages

450-8

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

148

pub_type

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