Abstract:
:For the pathogenic yeast Candida albicans, secreted aspartyl proteinase (Sap) activity has been correlated with virulence. A family consisting of at least eight SAP genes can be drawn upon to produce Sap enzymatic activity. In this study, the levels of Sap1, Sap2, and Sap3 isoenzymes were monitored under a variety of growth conditions for several strains, including strain WO-1, which alternates between two switch phenotypes, white (W) and opaque (O). When cultured under proteinase-inducing conditions, most strains and W cells produce Sap2, while O cells produce Sap1, Sap2, and Sap3. Both W and O cells of strain WO-1 produce Saps in enriched and defined media that do not induce Saps from other strains. The specific Sap isoenzyme that is produced is determined by the cell type, while the level of Sap production is determined by environmental factors. The levels and temporal regulation of the SAP mRNAs as determined by Northern (RNA) analysis were consistent with Sap protein levels and with previous results. S1 analysis showed that SAP6 is the predominant SAP gene transcribed during hyphal induction at neutral pH. These studies define the culture conditions which control the levels of SAP mRNAs and Sap proteins, and they indicate that both the yeast/hyphal transition and phenotypic switching can determine which of the Sap isoenzymes is produced.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
White TC,Agabian Ndoi
10.1128/jb.177.18.5215-5221.1995subject
Has Abstractpub_date
1995-09-01 00:00:00pages
5215-21issue
18eissn
0021-9193issn
1098-5530journal_volume
177pub_type
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.2.527-537.1971
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abstract::A widely distributed family of small regulators, called C proteins, controls a subset of restriction-modification systems. The C proteins studied to date activate transcription of their own genes and that of downstream endonuclease genes; this arrangement appears to delay endonuclease expression relative to that of th...
journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/jb.169.9.4203-4210.1987
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.23.7549-7556.1991
更新日期:1991-12-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2005-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2016-08-25 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.156.1.362-368.1983
更新日期:1983-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.606-607.1970
更新日期:1970-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.690-693.1979
更新日期:1979-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.22.5844-5854.1998
更新日期:1998-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.15.4673-4675.1999
更新日期:1999-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.14.4243-4249.1994
更新日期:1994-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.8.4385-4394.1989
更新日期:1989-08-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.7.1793-1799.1996
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1989-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.2.375-380.1978
更新日期:1978-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.21.5619-5625.1998
更新日期:1998-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2004-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2009-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.21.6772-6778.1999
更新日期:1999-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.2.603-607.1980
更新日期:1980-11-01 00:00:00
abstract::Successful completion of the bacteriophage P2 lytic cycle requires phage-induced lysis of its Escherichia coli host, a process that is poorly understood. Genetic analysis of lysis-deficient mutants defined a single locus, gene K, which lies within the largest late transcription unit of P2 and maps between head gene L ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.16.4974-4984.1994
更新日期:1994-08-01 00:00:00
abstract::The site of integration and the molecular orientation of a prophage Mu integrated within the resistance transfer factor component of plasmid R100-1 have been determined on the physical map of the plasmid. This allowed us (i) to determine the direction of deoxyribonucleic acid transfer from oriT during conjugation and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.3.1105-1108.1979
更新日期:1979-12-01 00:00:00