Transposition of Tn7 occurs at a single site on the Caulobacter crescentus chromosome.

abstract：:The cheA locus of Escherichia coli encodes two similar proteins, CheAL (654 amino acids) and CheAS (557 amino acids), which are made by initiating translation from different in-frame start sites [start(L) and start(S)]. CheAL plays an essential role in chemotactic signaling. It autophosphorylates at a histidine residu...

journal_title：Journal of bacteriology

authors： Sanatinia H,Kofoid EC,Morrison TB,Parkinson JS

更新日期：1995-05-01 00:00:00

abstract：:Vibrio alginolyticus contained two adjacent genes, ktrA and ktrB, which encode a new type of bacterial K(+)-uptake system. KtrA and KtrB are peripheral and integral membrane proteins, respectively. Six of the nine sequenced bacterial genomes contain homologs to both ktrA and ktrB, suggesting that KtrAB is widespread. ...

journal_title：Journal of bacteriology

authors： Nakamura T,Yuda R,Unemoto T,Bakker EP

更新日期：1998-07-01 00:00:00

abstract：:Streptococcus faecalis strain JH1 harbors two conjugative plasmids: pJH1, an R plasmid mediating resistance to kanamycin, streptomycin, gentamicin, erythromycin, and tetracycline, and pJH2, a hemolysin-bacteriocin plasmid. Studies of plasmid-cured derivatives of strain JH1 and of transconjugates obtained after mixed i...

journal_title：Journal of bacteriology

authors： LeBlanc DJ,Lee LN

更新日期：1982-05-01 00:00:00

abstract：:We show that both flanking IS256 elements carried by transposon Tn4001 are capable of generating head-to-tail tandem copies and free circular forms, implying that both are active. Our results suggest that the tandem structures arise from dimeric copies of the donor or vector plasmid present in the population by a mech...

journal_title：Journal of bacteriology

authors： Prudhomme M,Turlan C,Claverys JP,Chandler M

更新日期：2002-01-01 00:00:00

abstract：:Mutants for 9 of the 10 steps in histidine biosynthesis have been isolated and identified by enzyme assay. Each locus has been mapped in relation to the aro cluster and to other histidine loci by deoxyribonucleic acid-mediated transformation. The genes which code for enzymes 3, 6, and 8 of the pathway are linked to th...

journal_title：Journal of bacteriology

authors： Chapman LF,Nester EW

更新日期：1969-03-01 00:00:00

abstract：:Genes involved in the biosynthesis of leucine have been mapped in Bacillus megaterium QM B1551, using transducing phage MP13. Mutations were designated leuA, leuB, or leuC on the basis of enzyme assays. Two mutant strains were deficient in the enzyme activities of leuA (alpha-isopropylmalate synthase) and leuC (beta-i...

journal_title：Journal of bacteriology

authors： Garbe JC,Hess GF,Franzen MA,Vary PS

更新日期：1984-02-01 00:00:00

abstract：:Zymomonas mobilis ZM401 is a flocculating strain which can be self-immobilized within fermentors for a high-cell-density culture to improve ethanol productivity, as well as high-gravity fermentation to increase ethanol titer, due to its improved ethanol tolerance associated with the morphological change. Here, we repo...

journal_title：Journal of bacteriology

authors： Zhao N,Bai Y,Zhao XQ,Yang ZY,Bai FW

更新日期：2012-12-01 00:00:00

abstract：:The secondary metabolite hydrogen cyanide (HCN) is produced by Pseudomonas fluorescens from glycine, essentially under microaerophilic conditions. The genetic basis of HCN synthesis in P. fluorescens CHA0 was investigated. The contiguous structural genes hcnABC encoding HCN synthase were expressed from the T7 promoter...

journal_title：Journal of bacteriology

authors： Laville J,Blumer C,Von Schroetter C,Gaia V,Défago G,Keel C,Haas D

更新日期：1998-06-01 00:00:00

abstract：:Lipopolysaccharide is a major glycolipid component in the outer leaflet of the outer membrane (OM), a peculiar permeability barrier of Gram-negative bacteria that prevents many toxic compounds from entering the cell. Lipopolysaccharide transport (Lpt) across the periplasmic space and its assembly at the Escherichia co...

journal_title：Journal of bacteriology

authors： Villa R,Martorana AM,Okuda S,Gourlay LJ,Nardini M,Sperandeo P,Dehò G,Bolognesi M,Kahne D,Polissi A

更新日期：2013-03-01 00:00:00

abstract：:The TON_0887 gene was identified as the missing histidinol-phosphate phosphatase (HolPase) in the hyperthermophilic archaeon "Thermococcus onnurineus" NA1. The protein contained conserved motifs of the DDDD superfamily of phosphohydrolase, and the recombinantly expressed protein exhibited strong HolPase activity. In t...

journal_title：Journal of bacteriology

authors： Lee HS,Cho Y,Lee JH,Kang SG

更新日期：2008-04-01 00:00:00

abstract：:Mycobacterium massiliense (Mycobacterium abscessus group) is an emerging pathogen causing pulmonary disease and skin and soft tissue infections. We report the genome sequence of the type strain CCUG 48898. ...

journal_title：Journal of bacteriology

authors： Tettelin H,Sampaio EP,Daugherty SC,Hine E,Riley DR,Sadzewicz L,Sengamalay N,Shefchek K,Su Q,Tallon LJ,Conville P,Olivier KN,Holland SM,Fraser CM,Zelazny AM

更新日期：2012-10-01 00:00:00

abstract：:The ability of deoxyribonucleic acid (DNA) isolated from a number of actinomycetes to reassociate with reference DNA from Actinoplanes philippinensis or Streptomyces venezuelae has been measured. All of the DNA preparations except for those from Nocardia erythropolis and Thermomonospora viridis contained 70 to 73 mole...

journal_title：Journal of bacteriology

authors： Farina G,Bradley SG

更新日期：1970-04-01 00:00:00

abstract：:An 8,883-bp mini-pXO1 plasmid containing a replicon from Bacillus anthracis pXO1 (181.6 kb) was identified by making large deletions in the original plasmid using a newly developed Cre-loxP system. Portions of the truncated mini-pXO1 were cloned into an Escherichia coli vector unable to replicate in B. anthracis. A 5....

journal_title：Journal of bacteriology

authors： Pomerantsev AP,Camp A,Leppla SH

更新日期：2009-08-01 00:00:00

abstract：:DNA damage and perturbations in DNA replication can induce global transcriptional responses that can help organisms repair the damage and survive. RecA is known to mediate transcriptional responses to DNA damage in several bacterial species by inactivating the repressor LexA and phage repressors. To gain insight into ...

journal_title：Journal of bacteriology

authors： Goranov AI,Kuester-Schoeck E,Wang JD,Grossman AD

更新日期：2006-08-01 00:00:00

abstract：:Nonpiliated, phage phi 6-resistant mutants of Pseudomonas syringae pv. phaseolicola were generated by Tn5 transposon mutagenesis. A P. syringae pv. phaseolicola LR700 cosmid library was screened with Tn5-containing EcoRI fragments cloned from nonpiliated mutants. The cosmid clone pVK253 complemented the nonpiliated mu...

journal_title：Journal of bacteriology

authors： Roine E,Nunn DN,Paulin L,Romantschuk M

更新日期：1996-01-01 00:00:00

abstract：:Concanavalin A-reactive linker and anchor subunits of phycobilisomes from Anacystis nidulans R2 (H. C. Riethman, T. P. Mawhinney, and L. A. Sherman, FEBS Lett. 215:209-214, 1987) were purified electrophoretically and analyzed for carbohydrate composition and quantity. Different quantities of glucose and N-acetylgalact...

journal_title：Journal of bacteriology

authors： Riethman HC,Mawhinney TP,Sherman LA

更新日期：1988-06-01 00:00:00

abstract：:The tar and tap loci of Escherichia coli encode methyl-accepting inner membrane proteins that mediate chemotactic responses to aspartate and maltose or to dipeptides. These genes lie adjacent to each other in the same orientation on the chromosome and have extensive sequence homology throughout the C-terminal portions...

journal_title：Journal of bacteriology

authors： Slocum MK,Halden NF,Parkinson JS

更新日期：1987-07-01 00:00:00

abstract：:The expression of any given bacterial protein is predicted to depend on (i) the transcriptional regulation of the promoter and the translational regulation of its mRNA and (ii) the synthesis and translation of total (bulk) mRNA. This is because total mRNA acts as a competitor to the specific mRNA for the binding of in...

journal_title：Journal of bacteriology

authors： Liang ST,Xu YC,Dennis P,Bremer H

更新日期：2000-06-01 00:00:00

abstract：:Escherichia coli K antigens (capsular polysaccharides) are divided into two broad classes, designated groups I and II, on the basis of a number of chemical, physical, and genetic criteria. Group I K antigens can be further subdivided on the basis of the absence (group IA) or presence (group IB) of amino sugars in the ...

journal_title：Journal of bacteriology

authors： MacLachlan PR,Keenleyside WJ,Dodgson C,Whitfield C

更新日期：1993-12-01 00:00:00

abstract：:Protein glycosylation is an important posttranslational modification that occurs in all domains of life. Pilins, the structural components of type IV pili, are O glycosylated in Neisseria meningitidis, Neisseria gonorrhoeae, and some strains of Pseudomonas aeruginosa. In this work, we characterized the P. aeruginosa 1...

journal_title：Journal of bacteriology

authors： Faridmoayer A,Fentabil MA,Mills DC,Klassen JS,Feldman MF

更新日期：2007-11-01 00:00:00

abstract：:The stator of the sodium-driven flagellar motor of Vibrio alginolyticus is a membrane protein complex composed of four PomA and two PomB subunits. PomB has a peptidoglycan-binding motif in the C-terminal region. In this study, four kinds of PomB deletions in the C terminus were constructed. None of the deletion protei...

journal_title：Journal of bacteriology

authors： Yakushi T,Hattori N,Homma M

更新日期：2005-01-01 00:00:00

abstract：:Genes from Bacillus subtilis predicted to encode a phosphate-specific transport (Pst) system were shown by mutation to affect high-affinity Pi uptake but not arsenate resistance or phosphate (Pho) regulation. The transcription start of the promoter upstream of the pstS gene was defined by primer extension. The promote...

journal_title：Journal of bacteriology

authors： Qi Y,Kobayashi Y,Hulett FM

更新日期：1997-04-01 00:00:00

abstract：:Controlled acetolysis of yeast mannans yields mixtures of oligosaccharides with (1-->2) and (1-->3) linkages between the mannose units, whereas the less stable (1-->6) linkages of the polysaccharide backbone are cleaved. The "fingerprints," obtained by gel filtration of the oligosaccharide mixtures, can be used to dis...

journal_title：Journal of bacteriology

authors： Kocourek J,Ballou CE

更新日期：1969-12-01 00:00:00

abstract：:Bacillus subtilis JH642 and a wild strain of B. subtilis called 22a both produce an antilisterial peptide that can be purified by anion-exchange and gel filtration chromatography. Amino acid analysis confirmed that the substance was the cyclic bacteriocin subtilosin. A mutant defective in production of the substance w...

journal_title：Journal of bacteriology

authors： Zheng G,Yan LZ,Vederas JC,Zuber P

更新日期：1999-12-01 00:00:00

abstract：:The gene encoding an extracellular isomalto-dextranase, designated imd, was isolated from the chromosomal DNA of Arthrobacter globiformis T6 and cloned and expressed in Escherichia coli. A single open reading frame consisting of 1,926 base pairs that encoded a polypeptide composed of a signal peptide of 39 amino acids...

journal_title：Journal of bacteriology

authors： Iwai A,Ito H,Mizuno T,Mori H,Matsui H,Honma M,Okada G,Chiba S

更新日期：1994-12-01 00:00:00

abstract：:The activity of ribosomes from a clinical isolate of Escherichia coli, exposed to starvation for 7 days in sea salts medium, was investigated by measuring the kinetic parameters of ribosomal peptidyltransferase, by using the puromycin reaction as a model reaction. No alterations in the extent of peptide bond formation...

journal_title：Journal of bacteriology

authors： Kalpaxis DL,Karahalios P,Papapetropoulou M

更新日期：1998-06-01 00:00:00

abstract：:When cells of Streptococcus faecalis ATCC 9790 were incubated at temperatures above 10 C before being frozen for freeze-fracture, a random distribution of particles was observed on the outer fracture face of the freeze-cleaved cell membrane. However, when cells were incubated below 10 C before freezing, particleless p...

journal_title：Journal of bacteriology

authors： Tsien HC,Higgins ML

更新日期：1974-05-01 00:00:00

abstract：:The phs mutation, which causes a pleiotropic growth defect, has been mapped and shown to be an allele of rpoA, the gene for the alpha subunit of RNA polymerase. The mutation is shown to cause a transcription defect in the arabinose operon, araBAD. ...

journal_title：Journal of bacteriology

authors： Rowland GC,Giffard PM,Booth IR

更新日期：1985-11-01 00:00:00

abstract：:ClpX is a well-characterized bacterial chaperone that plays a role in many processes, including protein turnover and the remodeling of macromolecular complexes. All of these activities require ATP hydrolysis-dependent, ClpX-mediated protein unfolding. Here we used site-directed mutagenesis in combination with genetics...

journal_title：Journal of bacteriology

authors： Haeusser DP,Lee AH,Weart RB,Levin PA

更新日期：2009-03-01 00:00:00

abstract：:A gene (aad) coding for an aldehyde/alcohol dehydrogenase (AAD) was identified immediately upstream of the previously cloned ctfA (J. W. Cary, D. J. Petersen, E. T. Papoutsakis, and G. N. Bennett, Appl. Environ. Microbiol. 56:1576-1583, 1990) of Clostridium acetobutylicum ATCC 824 and sequenced. The 2,619-bp aad codes...

journal_title：Journal of bacteriology

authors： Nair RV,Bennett GN,Papoutsakis ET

更新日期：1994-02-01 00:00:00