Abstract:
:Nonpiliated, phage phi 6-resistant mutants of Pseudomonas syringae pv. phaseolicola were generated by Tn5 transposon mutagenesis. A P. syringae pv. phaseolicola LR700 cosmid library was screened with Tn5-containing EcoRI fragments cloned from nonpiliated mutants. The cosmid clone pVK253 complemented the nonpiliated mutant strain HB2.5. A 3.8-kb sequenced region spanning the Tn5 insertion site contained four open reading frames. The transposon-inactivated gene, designated pilP, is 525 bp long, potentially encoding a 19.1-kDa protein precursor that contains a typical membrane lipoprotein leader sequence. Generation of single mutations in each of the three remaining complete open reading frames by marker exchange also resulted in a nonpiliated phenotype. Expression of this gene region by the T7 expression system in Escherichia coli resulted in four polypeptides of approximately 39, 26, 23, and 18 kDa, in agreement with the sizes of the open reading frames. The three genes upstream of pilP were designated pilM (39 kDa), pilN (23 kDa), and pilO (26 kDa). The processing of the PilP precursor into its mature form was shown to be inhibited by globomycin, a specific inhibitor of signal peptidase II. The gene region identified shows a high degree of homology to a gene region reported to be required for Pseudomonas aeruginosa type IV pilus production.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Roine E,Nunn DN,Paulin L,Romantschuk Mdoi
10.1128/jb.178.2.410-417.1996subject
Has Abstractpub_date
1996-01-01 00:00:00pages
410-7issue
2eissn
0021-9193issn
1098-5530journal_volume
178pub_type
杂志文章abstract::We have cloned three copies of a repeated DNA segment from Borrelia burgdorferi sensu stricto strain B31, present on both circular and linear plasmids of this and other B. burgdorferi sensu lato strains. The DNA sequences are characterized by a highly homologous segment containing two open reading frames (ORFs), ORF-A...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2287-2298.1996
更新日期:1996-04-01 00:00:00
abstract::Escherichia coli sigma54-dependent phage shock protein operon (pspA to -E) transcription is under the control of PspF, a constitutively active activator. Sigma70-dependent transcription of pspF is under autogenous control by wild-type PspF but not by a DNA-binding mutant, PspF deltaHTH. Negative autoregulation of PspF...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.16.5232-5237.1997
更新日期:1997-08-01 00:00:00
abstract::In some media, Bacillus subtilis can maintain a prolonged stationary growth phase; however, in other media, nutrient depletion triggers a complex differentiation that culminates in production of a dormant endospore. This differentiation requires the expression of many genes. We found that during the stationary phase i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.709-715.1990
更新日期:1990-02-01 00:00:00
abstract::Peptidoglycan hydrolase, LytF (CwlE), was determined to be identical to YhdD (deduced cell wall binding protein) by zymography after insertional inactivation of the yhdD gene. YhdD exhibits high sequence similarity with CwlF (PapQ, LytE) and p60 of Listeria monocytogenes. The N-terminal region of YhdD has a signal seq...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.10.3178-3184.1999
更新日期:1999-05-01 00:00:00
abstract::The RNA polymerase sigma subunit, sigmaH, of Bacillus subtilis is required for the transcription of genes that are induced in late-growth cultures at high cell density, including genes that function in sporulation. The expression of sigmaH-controlled genes is repressed when nutrient broth sporulation medium (Difco spo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.21.6778-6787.1997
更新日期:1997-11-01 00:00:00
abstract::A mutant of Escherichia coli lacking hydroxyethylthiazole kinase (EC 2.7.1.50) was produced by a further mutation of a temperature-sensitive, auxotrophic mutant for hydroxyethylthiazole. The parent cells possessed two distinct enzymes capable of phosphorylating hydroxyethylthiazole: one was hydroxyethylthiazole kinase...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.3228-3232.1989
更新日期:1989-06-01 00:00:00
abstract::Proteus mirabilis is a dimorphic bacterium which exists in liquid cultures as a 1.5- to 2.0-microns motile swimmer cell possessing 6 to 10 peritrichous flagella. When swimmer cells are placed on a surface, they differentiate by a combination of events that ultimately produce a swarmer cell. Unlike the swimmer cell, th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.19.6279-6288.1991
更新日期:1991-10-01 00:00:00
abstract::At a pH of journal_title:Journal of bacteriology pub_type: 杂志文章 doi:10.1128/jb.184.19.5339-5347.2002 更新日期:2002-10-01 00:00:00
abstract::Rosenkranz, Herbert S. (Columbia University, New York, N.Y.), Howard S. Carr, and Harry M. Rose. Phenethyl alcohol. II. Effect on thymine-requiring Escherichia coli. J. Bacteriol. 89:1370-1373. 1965.-Phenethyl alcohol (PEA) at levels (0.05%) presumed to interfere with messenger ribonucleic acid synthesis also inhibits...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.5.1370-1373.1965
更新日期:1965-05-01 00:00:00
abstract::In the nitrogenase system of Klebsiella pneumoniae, nifT is located between nifDK, the structural genes for dinitrogenase, and nifY, whose product is involved in nitrogenase maturation. It is, therefore, a reasonable hypothesis that the NifT protein might also have a role in the maturation of nitrogenase. However, the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.10.2975-2977.1996
更新日期:1996-05-01 00:00:00
abstract::In studies of Pseudomonas putida IH-2000, a toluene-tolerant microorganism, membrane vesicles (MVs) were found to be released from the outer membrane when toluene was added to the culture. These MVs were found to be composed of phospholipids, lipopolysaccharides (LPS), and very low amounts of outer membrane proteins. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.22.6451-6455.2000
更新日期:2000-11-01 00:00:00
abstract::Accumulation of l-alpha-glycerophosphate, in cells of Bacillus subtilis mutants lacking the nicotinamide adenine dinucleotide-independent glycerophosphate dehydrogenase activity, suppresses both growth and sporulation. After growth has stopped, the cells slowly develop one and later more asymmetric septa that are thic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.2.1034-1045.1973
更新日期:1973-02-01 00:00:00
abstract::In Escherichia coli K-12, two enzymes, encoded by ilvE and tyrB, catalyze the amination of 2-ketoisocaproate (2-KIC) to form leucine. Although leucine-requiring derivatives of an ilvE strain that are unable to grow on 2-KIC were expected to have mutations only in tyrB, mapping studies showed that one such mutation was...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.12.3864-3871.1991
更新日期:1991-06-01 00:00:00
abstract::The antigenic specificity of Listeria monocytogenes types I, II, III, IVa, and IVb was studied by immunochemical techniques. Immunologically active carbohydrates of the various types were extracted from cell walls and were chemically analyzed. Types I and II contained predominantly glucosamine and rhamnose; type III, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.2.486-493.1969
更新日期:1969-05-01 00:00:00
abstract::The direct binding of Streptococcus mitis to human platelets is mediated in part by two proteins (PblA and PblB) encoded by a lysogenic bacteriophage (SM1). Since SM1 is the first prophage of S. mitis that has been identified and because of the possible role of these phage-encoded proteins in virulence, we sought to c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.23.6968-6975.2003
更新日期:2003-12-01 00:00:00
abstract::The phylum Bacteroidetes is large and diverse, with rapid gliding motility and the ability to digest macromolecules associated with many genera and species. Recently, a novel protein secretion system, the Por secretion system (PorSS), was identified in two members of the phylum, the gliding bacterium Flavobacterium jo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01962-12
更新日期:2013-01-01 00:00:00
abstract::The gene responsible for the uncommon glutamate dehydrogenase (GDH) activity of Lactococcus lactis was identified and characterized. It encodes a GDH of family I that is mainly active in glutamate biosynthesis, is carried by a large plasmid, and is included, with functional cadmium resistance genes, in a remnant Tn3-l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.14.5019-5022.2005
更新日期:2005-07-01 00:00:00
abstract::Considerable amounts of immunoglobulin G (IgG) antibody appeared in hyperimmune rabbit serum at a late period during a course of immunization with several injections of Shigella flexneri O antigens. High yields of IgG antibody possessing homogenous specificity could be fractionated from crude gamma-globulin solution o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.3.1128-1134.1969
更新日期:1969-06-01 00:00:00
abstract::The Streptomyces coelicolor absA two-component system was initially identified through analysis of mutations in the sensor kinase absA1 that caused inhibition of all four antibiotics synthesized by this strain. Previous genetic analysis had suggested that the phosphorylated form of AbsA2 acted as a negative regulator ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.3.794-805.2002
更新日期:2002-02-01 00:00:00
abstract::The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a carboxyl-terminal regulatory domain (IIALacS) that is homologous to a family of proteins and protein domains of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) in various organisms, of which IIAGlc of Escherichia coli is the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.2.632-641.1999
更新日期:1999-01-01 00:00:00
abstract::Bacillus subtilis contains three Fur homologs: Fur, PerR, and Zur. Despite significant sequence similarities, they respond to different stimuli and regulate different sets of genes. DNA target site comparisons indicate that all three paralogs recognize operators with a core 7-1-7 inverted repeat. The corresponding con...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.21.6348-6357.2003
更新日期:2003-11-01 00:00:00
abstract::Tn5 was used to generate mutants that were deficient in the dissimilatory reduction of nitrite for Pseudomonas sp. strain G-179, which contains a copper nitrite reductase. Three types of mutants were isolated. The first type showed a lack of growth on nitrate, nitrite, and nitrous oxide. The second type grew on nitrat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.20.6653-6658.1992
更新日期:1992-10-01 00:00:00
abstract::The predicted amino acid sequence of Bacillus subtilis ycsK exhibits similarity to the GDSL family of lipolytic enzymes. Northern blot analysis showed that ycsK mRNA was first detected from 4 h after the onset of sporulation and that transcription of ycsK was dependent on SigK and GerE. The fluorescence of the YcsK-gr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01527-06
更新日期:2007-03-01 00:00:00
abstract::The fermentative alcohol dehydrogenase of Escherichia coli is encoded by the adhE gene, which is induced under anaerobic conditions but repressed in air. Previous work suggested that induction of adhE might depend on NADH levels. We therefore directly measured the NAD+ and NADH levels for cultures growing aerobically ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.20.6013-6018.1996
更新日期:1996-10-01 00:00:00
abstract::Abram, Dinah (Purdue University, Lafayette, Ind.), A. E. Vatter, and Henry Koffler. Attachment and structural features of flagella of certain bacilli. J. Bacteriol. 91:2045-2068. 1966.-The attachment of flagella to cells of various mesophilic and thermophilic strains of Bacillus was studied electron microscopically. S...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.5.2045-2068.1966
更新日期:1966-05-01 00:00:00
abstract::Cells of the cyanobacterium Anabaena variabilis starved for phosphate for 3 days took up phosphate at about 100 times the rate of unstarved cells. Kinetic data suggested that a new transport system had been induced by starvation for phosphate. The inducible phosphate transport system was quickly repressed by addition ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.3.1143-1147.1988
更新日期:1988-03-01 00:00:00
abstract::The bacteriocin produced by a strain of Staphylococcus aureus has been isolated and designated staphylococcin (414), and a study was made of its chemical, physical, and biological properties. The staphylococcin is released in appreciable quantities after breakage of the cells and can be purified through differential c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.117-125.1970
更新日期:1970-10-01 00:00:00
abstract::Enteropathogenic Escherichia coli employs a type III secretion system (T3SS) to translocate virulence effector proteins directly into enterocyte host cells, leading to diarrheal disease. The T3SS is encoded within the chromosomal locus of enterocyte effacement (LEE). The function of some of the LEE-encoded proteins re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01215-12
更新日期:2012-11-01 00:00:00
abstract::At various stages during spore formation sporangia were shocked by cold treatment or with toluene, and the germination requirements of the prespores were examined. Up to 5 h after induction of sporulation (t5) germination was spontaneous; i.e., it occurred without any added germinants. After t5, during stages V and VI...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.2.786-792.1980
更新日期:1980-02-01 00:00:00
abstract::The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and pUB307 were transferred to acidophilic, obligately chemolithotrophic Thiobacillus ferrooxidans from Escherichia coli by conjugation. A genetic marker of kanamycin resistance was expressed in T. ferrooxidans. Plasmid RP4 was transferred back to E. coli fro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.10.2892-2897.1994
更新日期:1994-05-01 00:00:00