Abstract：
:Bacillus subtilis contains three Fur homologs: Fur, PerR, and Zur. Despite significant sequence similarities, they respond to different stimuli and regulate different sets of genes. DNA target site comparisons indicate that all three paralogs recognize operators with a core 7-1-7 inverted repeat. The corresponding consensus sequences are identical at five or more of the seven defined positions. Using site-directed mutagenesis, the Per box at the mrgA promoter was altered to mimic the core 7-1-7 motif of the Fur and Zur boxes. In vitro, the mrgA promoter containing a Zur box was only recognized by Zur, as demonstrated by DNase I footprinting assays. In contrast, both Fur and PerR bound to the mrgA promoter region containing a consensus Fur box. Expression analysis of these promoters is consistent with the in vitro data demonstrating as few as 1 or 2 base changes per half-site are sufficient to alter regulation. Similarly, the Fur box at the feuA promoter can be converted into a Per or a Zur box by appropriate mutations. While both Fur and PerR could recognize some of the same synthetic operator sequences, no naturally occurring sites are known that are subject to dual regulation. However, the PerR-regulated zosA gene is controlled from a regulatory region that contains both Per and Fur boxes. Although purified Fur protein bound to the candidate Fur boxes, Fur has little effect on zosA expression-possibly due to the location of the Fur boxes relative to the zosA promoter. Together, our results identify two nucleotide positions that are important for the ability of PerR, Fur, and Zur to distinguish among the many closely related operator sites present in the B. subtilis genome.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Fuangthong M,Helmann JDdoi
10.1128/jb.185.21.6348-6357.2003keywords:
subject
Has Abstractpub_date
2003-11-01 00:00:00pages
6348-57issue
21eissn
0021-9193issn
1098-5530journal_volume
185pub_type
杂志文章abstract：:narK mutants of Escherichia coli produce wild-type levels of nitrate reductase but, unlike the wild-type strain, do not accumulate nitrite when grown anaerobically on a glucose-nitrate medium. Comparison of the rates of nitrate and nitrite metabolism in cultures growing anaerobically on glucose-nitrate medium revealed...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.173.11.3303-3310.1991
更新日期：1991-06-01 00:00:00
abstract：:Five lacY mutants with amber stop codons at known positions were each placed into 12 different suppressor strains. The 60 amino acid substitutions obtained in this manner were tested for growth on lactose-minimal medium plates and for transport of lactose, melibiose, and thiomethylgalactoside. Most of the amino acid s...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.174.16.5436-5441.1992
更新日期：1992-08-01 00:00:00
abstract：:THREE PREVIOUSLY STUDIED R FACTORS WERE USED: 222/R4, controlling transmissible resistance to sulfonamide, streptomycin, chloromycetin, and tetracycline (SU(r) SM(r) CM(r) TC(r)); 222/R3, a derivative of 222/R4 (now termed 222/R3W) having lost TC(r); and R15, controlling infectious resistance to SU and SM only. Two ty...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.103.1.166-177.1970
更新日期：1970-07-01 00:00:00
abstract：:Current mouse models for evaluating the efficacy of live oral cholera vaccines (OCVs) have important limitations. Conventionally raised adult mice are resistant to intestinal colonization by Vibrio cholerae, but germfree mice can be colonized and have been used to study OCV immunogenicity. However, germfree animals ha...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.00232-20
更新日期：2020-11-19 00:00:00
abstract：:Nakata, H. M. (Washington State University, Pullman). Effect of pH on intermediates produced during growth and sporulation of Bacillus cereus. J. Bacteriol. 86:577-581. 1963.-Cultures of Bacillus cereus strain T, grown in an unbuffered glucose-yeast extract-mineral salts medium and in the same medium buffered at pH 6....
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.86.3.577-581.1963
更新日期：1963-09-01 00:00:00
abstract：:Single spores of Saccharomyces cerevisiae were examined during germination and outgrowth by scanning electron and phase-contrast microscopy. Also determined were changes in cell weight and light absorbance, trehalose utilization, and synthesis of protein and KOH-soluble carbohydrates. These studies reveal that develop...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.109.3.1232-1238.1972
更新日期：1972-03-01 00:00:00
abstract：:Yeast and hyphal walls of Candida albicans were extracted with sodium dodecyl sulfate (SDS). Some of the extracted proteins reacted with a specific beta-1,6-glucan antiserum but not with a beta-1,3-glucan antiserum. They lost their beta-1,6-glucan epitope after treatment with ice-cold aqueous hydrofluoric acid, sugges...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.177.13.3788-3792.1995
更新日期：1995-07-01 00:00:00
abstract：:Bacteriophage phi29 protein p6 is a viral architectural protein, which binds along the whole linear phi29 DNA in vivo and is involved in initiation of DNA replication and transcription control. Protein p1 is a membrane-associated viral protein, proposed to attach the viral genome to the cell membrane. Protein p17 is i...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.186.24.8401-8406.2004
更新日期：2004-12-01 00:00:00
abstract：:Lysogenization of Salmonella typhimurium with either of the bacteriophages A3 and A4 results in O-acetylation of the L-rhamnose residues of the O-polysaccharide chain of the lipopolysaccharide of the bacterial cell envelope. The O-acetyl group is found on both O-2 and O-3 of the L-rhamnosyl residues. This lysogenic co...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.169.3.1003-1009.1987
更新日期：1987-03-01 00:00:00
abstract：:Acyl carrier protein (ACP coli) was isolated from commercially grown Escherichia coli B and was acetylated by chemical methods. Biological activity of the synthesized acetyl-ACP coli was checked in an in vitro fatty acid-synthesizing system isolated from E. coli B. Since acetyl-ACP is preferred over acetyl-coenzyme A ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.96.4.1281-1284.1968
更新日期：1968-10-01 00:00:00
abstract：:Synthesis of the Salmonella typhimurium hook protein from the gene cloned on a multicopy plasmid results in partial suppression of the flagellar assembly defects of certain classes of Escherichia coli mutants (K. Ohnishi, M. Homma, K. Kutsukake, and T. Iino, J. Bacteriol, 169:1485-1488, 1987). This phenomenon allowed ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.169.4.1489-1492.1987
更新日期：1987-04-01 00:00:00
abstract：:NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not a...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.177.1.247-251.1995
更新日期：1995-01-01 00:00:00
abstract：:The gene coding for the Escherichia coli enzyme 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase has been cloned and sequenced. This gene, designated folK, codes for a protein of 159 amino acids, including an amino-terminal methionine. The protein was overexpressed in E. coli MC4100 by cloning the gene behind the l...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.174.18.5971-5977.1992
更新日期：1992-09-01 00:00:00
abstract：:The respiratory control of adhE, which encodes ethanol (alcohol) dehydrogenase in Escherichia coli, was examined at the transcriptional level by using various phi (adhE'-lacZ) adhE+ merodiploid strains. Expression of the adhE'-lacZ operon fusion was increased about eightfold by anaerobic growth. Under anaerobic growth...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.173.24.8009-8013.1991
更新日期：1991-12-01 00:00:00
abstract：:The plasmid-encoded yop genes of pathogenic yersiniae are regulated by the environmental stimuli calcium and temperature. A novel protein, LcrQ, which exhibits a key function in the negative calcium-controlled pathway, was identified. DNA sequence analysis revealed that LcrQ has a molecular mass of 12,412 daltons and ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.174.10.3355-3363.1992
更新日期：1992-05-01 00:00:00
abstract：:Parnas, Józef (Academy of Medicine, Lublin, Poland). Differentiation of brucellae by the aid of phages. J. Bacteriol. 82:319-325. 1961.-There appears to be no absolute specificity of phages for the three species of the genus Brucella. The phage test is only supplementary to biochemical and serological methods for spec...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.82.3.319-325.1961
更新日期：1961-09-01 00:00:00
abstract：:The Escherichia coli K42 capsular polysaccharide consists of leads to 3)-alpha-D-Galp-(1 leads to 3)-alpha-D-GalUAp-(1 leads to 3)-alpha-L-Fucp-(1 leads to repeating units. The E. coli K42 and Klebsiella K63 antigens are serologically identical. ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.133.1.390-391.1978
更新日期：1978-01-01 00:00:00
abstract：:Bulk membrane fragments were prepared from cells of Bacillus cereus ATCC 4342 harvested at different stages of growth and sporulation and examined for enzymes involved in electron transport functions. The presence of succinate: DCPIP oxidoreductase (EC 1.3.99.1), succinate: cytochrome c oxidoreductase (EC 1.3.2.1), NA...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.110.3.968-977.1972
更新日期：1972-06-01 00:00:00
abstract：:During sporulation, the Bacillus subtilis transcription factor sigmaK is activated by regulated proteolytic processing. I have used a system that facilitates the analysis of the contributions of a modified form of the processing enzyme, SpoIVFB-GFP, and the regulatory proteins BofA and SpoIVFA to the conversion of pro...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.181.17.5384-5388.1999
更新日期：1999-09-01 00:00:00
abstract：:D-Ribose isomerase was purified and crystallized from cells of Mycobacterium smegmatis grown on either D-ribose or L-rhamnose. Isomerase activity for both of these sugars remained together throughout the purification. The isomerase from L-rhamnose-grown cells had the same chemical and physical properties as the enzyme...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.126.1.553-555.1976
更新日期：1976-04-01 00:00:00
abstract：:The vir locus of Bordetella pertussis apparently encodes a trans-acting positive regulator that is required for the coordinate expression of genes associated with virulence: pertussis toxin, filamentous hemagglutinin (FHA), hemolysin, and adenylate cyclase toxin. DNA clones of vir and of genes required for the synthes...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.170.7.2904-2913.1988
更新日期：1988-07-01 00:00:00
abstract：:The genome of Lactobacillus salivarius UCC118 includes a 242-kb megaplasmid, pMP118. We now show that 33 strains of L. salivarius isolated from humans and animals all harbor a megaplasmid, which hybridized with the repA and repE replication origin probes of pMP118. Linear megaplasmids that did not hybridize with the p...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.00447-07
更新日期：2007-09-01 00:00:00
abstract：:Escherichia coli has been used as a platform host for studying the production of free fatty acids (FFA) and other energy-dense compounds useful in biofuel applications. Most of the FFA produced by E. coli are found extracellularly. This finding suggests that a mechanism for transport across the cell envelope exists, y...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.01477-12
更新日期：2013-01-01 00:00:00
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journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.01194-07
更新日期：2008-06-01 00:00:00
abstract：:The secretomes of a virulent Bacillus anthracis strain and of avirulent strains (cured of the virulence plasmids pXO1 and pXO2), cultured in rich and minimal media, were studied by a comparative proteomic approach. More than 400 protein spots, representing the products of 64 genes, were identified, and a unique patter...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.188.10.3551-3571.2006
更新日期：2006-05-01 00:00:00
abstract：:During insect infection Photorhabdus luminescens emits light and expresses virulence factors, including insecticidal toxin complexes (Tcs) and an RTX-like metalloprotease (Prt). Using quantitative PCR and protein assays, we describe the expression patterns of these factors both in culture and during insect infection a...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.183.20.5834-5839.2001
更新日期：2001-10-01 00:00:00
abstract：:Hurwitz, Charles (Veterans Administration Hospital, Albany, N.Y.) and Carmen L. Rosano. Chloramphenicol-sensitive and -insensitive phases of the lethal action of streptomycin. J. Bacteriol. 83:1202-1209. 1962.-If chloramphenicol is added to sensitive Escherichia coli cells at the same time as streptomycin, the lethal ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.83.6.1202-1209.1962
更新日期：1962-06-01 00:00:00
abstract：:The mobilizable Bacteroides element NBU2 (11 kbp) was found originally in two Bacteroides clinical isolates, Bacteroides fragilis ERL and B. thetaiotaomicron DOT. At first, NBU2 appeared to be very similar to another mobilizable Bacteroides element, NBU1, in a 2.5-kbp internal region, but further examination of the fu...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.182.12.3559-3571.2000
更新日期：2000-06-01 00:00:00
abstract：:Wzc(CPS) is a tyrosine autokinase essential for the assembly of a high-molecular-weight (HMW) group 1 capsular polysaccharide (CPS) in Escherichia coli. Homologues of Wzc participate in the formation of CPS and exopolysaccharides in a variety of gram-positive and gram-negative bacteria. Phosphorylation of tyrosine res...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.184.23.6437-6447.2002
更新日期：2002-12-01 00:00:00
abstract：:Bacteria often encounter environments where nutrient availability is limited, and they must adapt accordingly. To identify Pasteurella multocida genes that are differentially expressed during nutrient limitation, we utilized whole-genome microarrays to compare levels of gene expression during growth in rich and minima...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.184.13.3734-3739.2002
更新日期：2002-07-01 00:00:00
