Abstract:
:During insect infection Photorhabdus luminescens emits light and expresses virulence factors, including insecticidal toxin complexes (Tcs) and an RTX-like metalloprotease (Prt). Using quantitative PCR and protein assays, we describe the expression patterns of these factors both in culture and during insect infection and compare them to the associated bacterial growth curves. In culture, light and active Prt protease are produced in stationary phase. Tca also appears in stationary phase, whereas Tcd is expressed earlier. These patterns seen in a culture flask are strikingly similar to those observed during insect infection. Thus, in an infected insect, bacteria grow exponentially until the time of insect death at approximately 48 h, when both light and the virulence factors Prt protease and Tca are produced. In contrast, Tcd appears much earlier in insect infection. However, at present, the biological significance of this difference in timing of the production of the two toxins in unclear. This is the first documentation of the expression of Tcs and Prt in an insect and highlights the malleability of Photorhabdus as a model system for bacterial infection.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Daborn PJ,Waterfield N,Blight MA,Ffrench-Constant RHdoi
10.1128/JB.183.20.5834-5839.2001keywords:
subject
Has Abstractpub_date
2001-10-01 00:00:00pages
5834-9issue
20eissn
0021-9193issn
1098-5530journal_volume
183pub_type
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