Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth.

Abstract:

:NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not able to grow on methanol, methylamine, or choline, while heterotrophic growth is not influenced by the mutation. This finding indicates that GD-FALDH of P. denitrificans is essential for the oxidation of formaldehyde produced during methylotrophic growth.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ras J,Van Ophem PW,Reijnders WN,Van Spanning RJ,Duine JA,Stouthamer AH,Harms N

doi

10.1128/jb.177.1.247-251.1995

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

247-51

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

177

pub_type

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