Abstract:
:NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not able to grow on methanol, methylamine, or choline, while heterotrophic growth is not influenced by the mutation. This finding indicates that GD-FALDH of P. denitrificans is essential for the oxidation of formaldehyde produced during methylotrophic growth.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ras J,Van Ophem PW,Reijnders WN,Van Spanning RJ,Duine JA,Stouthamer AH,Harms Ndoi
10.1128/jb.177.1.247-251.1995subject
Has Abstractpub_date
1995-01-01 00:00:00pages
247-51issue
1eissn
0021-9193issn
1098-5530journal_volume
177pub_type
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更新日期:2019-04-09 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:1984-08-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.2.835-843.1982
更新日期:1982-05-01 00:00:00
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更新日期:2013-02-01 00:00:00
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更新日期:1962-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-08-01 00:00:00
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更新日期:1985-03-01 00:00:00
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更新日期:1964-02-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1995-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2007-10-01 00:00:00
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更新日期:2012-06-01 00:00:00
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更新日期:1990-03-01 00:00:00
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更新日期:1970-04-01 00:00:00
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更新日期:1967-04-01 00:00:00
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更新日期:1997-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1974-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1991-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.167.3.992-998.1986
更新日期:1986-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1997-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1999-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.418-423.1979
更新日期:1979-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01215-12
更新日期:2012-11-01 00:00:00