Isolation and partial characterization of protein E, a major protein found in certain Escherichia coli K-12 mutant strains: relationship to other outer membrane proteins.

Abstract:

:Escherichia coli outer membrane protein E was purified, and its amino acid composition and N-terminal amino acid were determined. The purified protein was shown to be immunologically and electrophoretically identical to proteins Ic (U. Henning, W. Schmidmayr, and I. Hindennach, Mol. Gen. Genet. 154:293-298, 1977) and e (W. van Alphen, N. van Selm, and B. Lugtenberg, Mol. Gen. Genet. 159:75-83, 1978). Proteins E, e, and Ic were also immunologically related to E. coli outer membrane protein Ia. Lugtenberg and co-workers (B. Lugtenberg, R. van Boxtel, C. Verhoef, and W. van Alphen, FEBS Lett. 96:99-105, 1978) have shown that electrophoretically identical peptides were generated by cyanogen bromide treatment of proteins E, e, and Ic.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Chai TJ,Foulds J

doi

10.1128/JB.139.2.418-423.1979

subject

Has Abstract

pub_date

1979-08-01 00:00:00

pages

418-23

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

139

pub_type

杂志文章
  • Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes.

    abstract::We present the complete genome sequence of Listeria welshimeri, a nonpathogenic member of the genus Listeria. Listeria welshimeri harbors a circular chromosome of 2,814,130 bp with 2,780 open reading frames. Comparative genomic analysis of chromosomal regions between L. welshimeri, Listeria innocua, and Listeria monoc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00758-06

    authors: Hain T,Steinweg C,Kuenne CT,Billion A,Ghai R,Chatterjee SS,Domann E,Kärst U,Goesmann A,Bekel T,Bartels D,Kaiser O,Meyer F,Pühler A,Weisshaar B,Wehland J,Liang C,Dandekar T,Lampidis R,Kreft J,Goebel W,Chakraborty

    更新日期:2006-11-01 00:00:00

  • Natural Strain Variation Reveals Diverse Biofilm Regulation in Squid-Colonizing Vibrio fischeri.

    abstract::The mutualistic symbiont Vibrio fischeri builds a symbiotic biofilm during colonization of squid hosts. Regulation of the exopolysaccharide component, termed Syp, has been examined in strain ES114, where production is controlled by a phosphorelay that includes the inner membrane hybrid histidine kinase RscS. Most stra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00033-19

    authors: Rotman ER,Bultman KM,Brooks JF 2nd,Gyllborg MC,Burgos HL,Wollenberg MS,Mandel MJ

    更新日期:2019-04-09 00:00:00

  • Bacteriophage infection in rod-shaped gram-positive bacteria: evidence for a preferential polar route for phage SPP1 entry in Bacillus subtilis.

    abstract::Entry into the host bacterial cell is one of the least understood steps in the life cycle of bacteriophages. The different envelopes of Gram-negative and Gram-positive bacteria, with a fluid outer membrane and exposing a thick peptidoglycan wall to the environment respectively, impose distinct challenges for bacteriop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05104-11

    authors: Jakutytė L,Baptista C,São-José C,Daugelavičius R,Carballido-López R,Tavares P

    更新日期:2011-09-01 00:00:00

  • Genetic selection and DNA sequences of 4.5S RNA homologs.

    abstract::A general strategy for cloning the functional homologs of an Escherichia coli gene was used to clone homologs of 4.5S RNA from other bacteria. The genes encoding these homologs were selected by their ability to complement a deletion of the gene for 4.5S RNA. DNA sequences of the regions encoding the homologs were dete...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.12.6517-6520.1989

    authors: Brown S,Thon G,Tolentino E

    更新日期:1989-12-01 00:00:00

  • Identification of a linked set of genes in Serpulina hyodysenteriae (B204) predicted to encode closely related 39-kilodalton extracytoplasmic proteins.

    abstract::A tandem pair of nearly identical genes from Serpulina hyodysenteriae (B204) were cloned and sequenced. The full open reading frame of one gene and the partial open reading frame of the neighboring gene appear to encode secreted proteins which are homologous to, yet distinct from, the 39-kDa extracytoplasmic protein p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.2.444-448.1998

    authors: Gabe JD,Dragon E,Chang RJ,McCaman MT

    更新日期:1998-01-01 00:00:00

  • Cloning, sequence analysis, and overexpression of Escherichia coli folK, the gene coding for 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase.

    abstract::The gene coding for the Escherichia coli enzyme 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase has been cloned and sequenced. This gene, designated folK, codes for a protein of 159 amino acids, including an amino-terminal methionine. The protein was overexpressed in E. coli MC4100 by cloning the gene behind the l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.18.5971-5977.1992

    authors: Talarico TL,Ray PH,Dev IK,Merrill BM,Dallas WS

    更新日期:1992-09-01 00:00:00

  • Identification and expression of the Escherichia coli fdhD and fdhE genes, which are involved in the formation of respiratory formate dehydrogenase.

    abstract::The two closely linked fdhD and fdhE genes of Escherichia coli are required for the formation of active membrane-bound phenazine methosulfate-linked formate dehydrogenase (FDH-PMS). Both genes were isolated from a cosmid library. Restriction endonuclease analysis associated with Mu dII1734 insertion mutagenesis indica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.10.6112-6121.1990

    authors: Schlindwein C,Giordano G,Santini CL,Mandrand MA

    更新日期:1990-10-01 00:00:00

  • Molecular properties of succinate dehydrogenase isolated from Micrococcus luteus (lysodeikticus).

    abstract::Succinate dehydrogenase (EC 1.3.99.1) of Micrococcus luteus was selectively precipitated from Triton X-100-solubilized membranes by using specific antiserum. The precipitated enzyme contained equimolar amounts of four polypeptides with apparent molecular weights of 72,000, 30,000, 17,000, and 15,000. The 72,000 polype...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.3.1493-1501.1983

    authors: Crowe BA,Owen P

    更新日期:1983-03-01 00:00:00

  • Diversity of Tn4001 transposition products: the flanking IS256 elements can form tandem dimers and IS circles.

    abstract::We show that both flanking IS256 elements carried by transposon Tn4001 are capable of generating head-to-tail tandem copies and free circular forms, implying that both are active. Our results suggest that the tandem structures arise from dimeric copies of the donor or vector plasmid present in the population by a mech...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.2.433-443.2002

    authors: Prudhomme M,Turlan C,Claverys JP,Chandler M

    更新日期:2002-01-01 00:00:00

  • Physiological studies of the regulation of beta-lactamase expression in Pseudomonas maltophilia.

    abstract::The kinetics of beta-lactamase induction in Pseudomonas maltophilia IID1275/873 were investigated. Upon induction with beta-lactam antibiotics, a correlation was seen between the increase in specific beta-lactamase activity and the generation time, as well as the concentration of inducer in the medium. The specific be...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.483-487.1989

    authors: Rosta S,Mett H

    更新日期:1989-01-01 00:00:00

  • Identification of FkpA as a key quality control factor for the biogenesis of outer membrane proteins under heat shock conditions.

    abstract::The outer membrane proteins (OMPs) of Gram-negative bacterial cells, as well as the mitochondrion and chloroplast organelles, possess unique and highly stable β-barrel structures. Biogenesis of OMPs in Escherichia coli involves such periplasmic chaperones as SurA and Skp. In this study, we found that the ΔsurA Δskp do...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01069-13

    authors: Ge X,Lyu ZX,Liu Y,Wang R,Zhao XS,Fu X,Chang Z

    更新日期:2014-02-01 00:00:00

  • A Mutation in the Bacillus subtilis rsbU Gene That Limits RNA Synthesis during Sporulation.

    abstract::Mutants of Bacillis subtilis that are temperature sensitive for RNA synthesis during sporulation were isolated after selection with a 32P suicide agent. Whole-genome sequencing revealed that two of the mutants carried an identical lesion in the rsbU gene, which encodes a phosphatase that indirectly activates SigB, the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00212-17

    authors: Rothstein DM,Lazinski D,Osburne MS,Sonenshein AL

    更新日期:2017-06-27 00:00:00

  • Plasmid-to-chromosome gene transfer in Haemophilus influenza during growth.

    abstract::A low level of recombination between homologous regions of plasmids and the host chromosome occurred during cell growth. The plasmids contained antibiotic resistance markers on the homologous regions which were only expressed when they were integrated into the chromosome. Such recombination took place in Rec+ rec-2 mu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.1.458-459.1986

    authors: Balganesh M,Arrigoni L,Setlow JK

    更新日期:1986-10-01 00:00:00

  • Independence of buoyant cell density and growth rate in Escherichia coli.

    abstract::The relationship between growth rate and buoyant density was determined for cells from exponential-phase cultures of Escherichia coli B/r NC32 by equilibrium centrifugation in Percoll gradients at growth rates ranging from 0.15 to 2.3 doublings per h. The mean buoyant density did not change significantly with growth r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.1.296-299.1984

    authors: Kubitschek HE,Baldwin WW,Schroeter SJ,Graetzer R

    更新日期:1984-04-01 00:00:00

  • Redox signal transduction by the ArcB sensor kinase of Haemophilus influenzae lacking the PAS domain.

    abstract::The Arc (anoxic redox control) two-component signal transduction system of Escherichia coli, which comprises the tripartite ArcB sensor kinase and the ArcA response regulator, modulates the expression of numerous operons in response to redox conditions of growth. We demonstrate that the arcA and arcB genes of Haemophi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.24.7206-7212.2001

    authors: Georgellis D,Kwon O,Lin EC,Wong SM,Akerley BJ

    更新日期:2001-12-01 00:00:00

  • Horizontal transference of S-layer genes within Thermus thermophilus.

    abstract::The S-layers of Thermus thermophilus HB27 and T. thermophilus HB8 are composed of protein units of 95 kDa (P95) and 100 kDa (P100), respectively. We have selected S-layer deletion mutants from both strains by complete replacement of the slpA gene. Mutants of the two strains showed similar defects in growth and morphol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.19.5460-5466.1995

    authors: Fernández-Herrero LA,Olabarría G,Castón JR,Lasa I,Berenguer J

    更新日期:1995-10-01 00:00:00

  • Phospholipids and lipopolysaccharide of Aeromonas hydrophila.

    abstract::The phospholipids and lipopolysaccharide of Aeromonas hydrophila were characterized. Phosphatidylethanolamine and phosphatidylglycerol were the major phospholipid components. The outer membrane contained more phosphatidylethanolamine and less phosphatidylglycerol than the inner membrane, and the phospholipids of the o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.463-465.1985

    authors: Howard SP,Buckley JT

    更新日期:1985-01-01 00:00:00

  • Identification of a novel host-specific IgM protease in Streptococcus suis.

    abstract::Streptococcus suis serotype 2 is a highly invasive, extracellular pathogen in pigs with the capacity to cause severe infections in humans. This study was initiated by the finding that IgM degradation products are released after opsonization of S. suis. The objective of this work was to identify the bacterial factor re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01875-12

    authors: Seele J,Singpiel A,Spoerry C,von Pawel-Rammingen U,Valentin-Weigand P,Baums CG

    更新日期:2013-03-01 00:00:00

  • Carbon dioxide fixation by cells of Streptococcus faecalis var. liquefaciens.

    abstract::Fixation of NaH(14)CO(3) by a heavy cell suspension of Streptococcus faecalis var. liquefaciens was studied. Several nutrients, pyridoxal, riboflavine, adenine, uracil, and O(2) stimulated (14)CO(2) incorporation into cells only under conditions that were adequate for synthesis of cell macromolecules. Biotin increased...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.27-33.1970

    authors: Goff RC,Hartman RE

    更新日期:1970-10-01 00:00:00

  • Suppression of early competence mutations in Bacillus subtilis by mec mutations.

    abstract::Although competence normally develops only in glucose-minimal salts media, mecA and mecB mutations permit the expression of competence and of late competence genes in complex media as well (D. Dubnau and M. Roggiani, J. Bacteriol. 172:4048-4055, 1990). The expression of late competence genes is dependent on the produc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.7.4056-4063.1990

    authors: Roggiani M,Hahn J,Dubnau D

    更新日期:1990-07-01 00:00:00

  • Cloning and sequencing of a cellobiose phosphotransferase system operon from Bacillus stearothermophilus XL-65-6 and functional expression in Escherichia coli.

    abstract::Cellulolytic strains of Bacillus stearothermophilus were isolated from nature and screened for the presence of activities associated with the degradation of plant cell walls. One isolate (strain XL-65-6) which exhibited strong activities with 4-methylumbelliferyl-beta-D-glucopyranoside (MUG) and 4-methylumbelliferyl-b...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.20.6441-6450.1993

    authors: Lai X,Ingram LO

    更新日期:1993-10-01 00:00:00

  • Localization of P42 and F(1)-ATPase α-subunit homolog of the gliding machinery in Mycoplasma mobile revealed by newly developed gene manipulation and fluorescent protein tagging.

    abstract::Mycoplasma mobile has a unique mechanism that enables it to glide on solid surfaces faster than any other gliding mycoplasma. To elucidate the gliding mechanism, we developed a transformation system for M. mobile based on a transposon derived from Tn4001. Modification of the electroporation conditions, outgrowth time,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01418-13

    authors: Tulum I,Yabe M,Uenoyama A,Miyata M

    更新日期:2014-05-01 00:00:00

  • Genomic analysis reveals chromosomal variation in natural populations of the uncultured psychrophilic archaeon Cenarchaeum symbiosum.

    abstract::Molecular phylogenetic surveys have recently revealed an ecologically widespread crenarchaeal group that inhabits cold and temperate terrestrial and marine environments. To date these organisms have resisted isolation in pure culture, and so their phenotypic and genotypic characteristics remain largely unknown. To cha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.19.5003-5009.1998

    authors: Schleper C,DeLong EF,Preston CM,Feldman RA,Wu KY,Swanson RV

    更新日期:1998-10-01 00:00:00

  • Method for localization of cloned DNA fragments on the Escherichia coli chromosome.

    abstract::In exponentially growing cultures of Escherichia coli strains carrying the dnaC28 mutation, DNA replication can be synchronized by temperature changes (R. L. Rodriguez, M. S. Dalbey, and C. I. Davern, J. Mol. Biol. 74:599-604, 1973). We used this synchronization procedure and DNA-DNA hybridization to develop a techniq...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.12.5641-5647.1987

    authors: Fayet O,Prere MF

    更新日期:1987-12-01 00:00:00

  • Novel xylose dehydrogenase in the halophilic archaeon Haloarcula marismortui.

    abstract::During growth of the halophilic archaeon Haloarcula marismortui on D-xylose, a specific D-xylose dehydrogenase was induced. The enzyme was purified to homogeneity. It constitutes a homotetramer of about 175 kDa and catalyzed the oxidation of xylose with both NADP+ and NAD+ as cosubstrates with 10-fold higher affinity ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.18.6198-6207.2004

    authors: Johnsen U,Schönheit P

    更新日期:2004-09-01 00:00:00

  • Characteristics of penicillinase secretion by growing cells and protoplasts of Bacillus licheniformis.

    abstract::Cultures of the inducible penicillinase-producing strain 749 of Bacillus licheniformis, induced with small amounts of benzylpenicillin, synthesized penicillinase at a high rate for a short period, after which the rate of synthesis slowly declined. During the period of active synthesis, the rate of secretion, as a frac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.2.820-826.1969

    authors: Sargent MG,Ghosh BK,Lampen JO

    更新日期:1969-02-01 00:00:00

  • Deletion strains reveal metabolic roles for key elemental sulfur-responsive proteins in Pyrococcus furiosus.

    abstract::Transcriptional and enzymatic analyses of Pyrococcus furiosus previously indicated that three proteins play key roles in the metabolism of elemental sulfur (S(0)): a membrane-bound oxidoreductase complex (MBX), a cytoplasmic coenzyme A-dependent NADPH sulfur oxidoreductase (NSR), and sulfur-induced protein A (SipA). D...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05445-11

    authors: Bridger SL,Clarkson SM,Stirrett K,DeBarry MB,Lipscomb GL,Schut GJ,Westpheling J,Scott RA,Adams MW

    更新日期:2011-12-01 00:00:00

  • Segregation of the replication terminus of the two Vibrio cholerae chromosomes.

    abstract::Genome duplication and segregation normally are completed before cell division in all organisms. The temporal relation of duplication and segregation, however, can vary in bacteria. Chromosomal regions can segregate towards opposite poles as they are replicated or can stay cohered for a considerable period before segr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.3.1060-1070.2006

    authors: Srivastava P,Fekete RA,Chattoraj DK

    更新日期:2006-02-01 00:00:00

  • EFFECT OF TEMPERATURE ON THE RATE OF GERMINATION IN BACILLUS CEREUS.

    abstract::Knaysi, Georges (Cornell University, Ithaca, N.Y.). Effect of temperature on the rate of germination in Bacillus cereus. J. Bacteriol. 87:619-622. 1964.-By use of the change of the spores from bright to dark, when observed in dark contrast with a phase microscope, as a criterion for the incipience (first stage) of ger...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.3.619-622.1964

    authors: KNAYSI G

    更新日期:1964-03-01 00:00:00

  • Genetic transformation of Streptococcus pneumoniae by heterologous plasmid deoxyribonucleic acid.

    abstract::A number of heterologous plasmid deoxyribonucleic acids (DNAs) coding for erythromycin, tylosin, lincomycin, tetracycline, or chloramphenicol resistance have been introduced into Streptococcus pneumoniae via genetic transformation with frequencies that varied between 10(-5) to as high as 5 x 10(-1) per colony-forming ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.2.698-709.1980

    authors: Barany F,Tomasz A

    更新日期:1980-11-01 00:00:00