Proline-dependent regulation of Clostridium difficile Stickland metabolism.

abstract：:Escherichia coli mutants lacking activities of all known cytosolic ATP-dependent proteases (Lon, ClpAP, ClpXP, and HslVU), due to double deletions [DeltahslVU and Delta(clpPX-lon)], cannot grow at low (30 degrees C) or very high (45 degrees C) temperatures, unlike those carrying either of the deletions. Such growth de...

journal_title：Journal of bacteriology

authors： Kanemori M,Yanagi H,Yura T

更新日期：1999-06-01 00:00:00

abstract：:Previously, we demonstrated that Escherichia coli tolC mutations reduce the high-level resistance to tetracycline afforded by the transposon Tn10-encoded TetA pump from resistance at 200 microg/ml to resistance at 40 microg/ml. In this study, we found that the addition of an sbmA mutation to a tolC::Tn10 mutant exacer...

journal_title：Journal of bacteriology

authors： de Cristóbal RE,Vincent PA,Salomón RA

更新日期：2008-02-01 00:00:00

abstract：:Pseudomonas stutzeri A1501 is a versatile nitrogen-fixing bacterium capable of living in diverse environments and coping with various oxidative stresses. NfiS, a regulatory noncoding RNA (ncRNA) involved in the control of nitrogen fixation in A1501, was previously shown to be required for optimal resistance to H2O2; h...

journal_title：Journal of bacteriology

authors： Zhang H,Zhan Y,Yan Y,Liu Y,Hu G,Wang S,Yang H,Qiu X,Liu Y,Li J,Lu W,Elmerich C,Lin M

更新日期：2019-09-06 00:00:00

abstract：:Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of convertin...

journal_title：Journal of bacteriology

authors： Römermann D,Friedrich B

更新日期：1985-05-01 00:00:00

abstract：:Tn5 was used to generate mutants that were deficient in the dissimilatory reduction of nitrite for Pseudomonas sp. strain G-179, which contains a copper nitrite reductase. Three types of mutants were isolated. The first type showed a lack of growth on nitrate, nitrite, and nitrous oxide. The second type grew on nitrat...

journal_title：Journal of bacteriology

authors： Ye RW,Averill BA,Tiedje JM

更新日期：1992-10-01 00:00:00

abstract：:Acyl carrier protein (ACP coli) was isolated from commercially grown Escherichia coli B and was acetylated by chemical methods. Biological activity of the synthesized acetyl-ACP coli was checked in an in vitro fatty acid-synthesizing system isolated from E. coli B. Since acetyl-ACP is preferred over acetyl-coenzyme A ...

journal_title：Journal of bacteriology

authors： Marcus SL,Reeves HC,Ajl SJ

更新日期：1968-10-01 00:00:00

abstract：:Low-copy-number plasmids, such as P1 prophage and the fertility factor F, require a plasmid-encoded replication protein and several host products for replication. Stable maintenance also depends on active partitioning of plasmids into daughter cells. Mini-P1 par+ and par plasmids were found to be destabilized by mutat...

journal_title：Journal of bacteriology

authors： Tilly K,Yarmolinsky M

更新日期：1989-11-01 00:00:00

abstract：:The number of rRNA genes in Mycobacterium bovis BCG was examined by Southern hybridization of end-labeled 5S, 16S, and 23S rRNAs with BamHI, PstI, and SalI digests of M. bovis BCG DNA. Each RNA probe gave only one radioactive band with three kinds of DNA digest. These results suggest that M. bovis BCG chromosomes may ...

journal_title：Journal of bacteriology

authors： Suzuki Y,Yoshinaga K,Ono Y,Nagata A,Yamada T

更新日期：1987-02-01 00:00:00

abstract：:We have identified a gene encoding a putative membrane protein homologous to the major facilitator superfamily, mapping upstream of the lacS gene in Sulfolobus solfataricus. Permeases from this family mediate secondary transport and are widely distributed among eubacteria and eukaryotes; the finding of an archaeal mem...

journal_title：Journal of bacteriology

authors： Prisco A,Moracci M,Rossi M,Ciaramella M

更新日期：1995-03-01 00:00:00

abstract：:Heterocyst differentiation is orchestrated by the N control transcriptional regulator NtcA and the differentiation-specific factor HetR. In Anabaena sp. strain PCC 7120, the devBCA operon is expressed from two different promoters activated upon N stepdown. The distal devB promoter (transcription start point [TSP] loca...

journal_title：Journal of bacteriology

authors： Camargo S,Valladares A,Flores E,Herrero A

更新日期：2012-06-01 00:00:00

abstract：:By partial acid hydrolysis, methylation and gas-liquid chromatography-mass spectrometry of the methylated monomers (as the alditol acetates), mass spectrometry of trimethylsilylated disaccharide alditols, as well as proton magnetic resonance, the primary structure of the Klebsiella serotype 25 capsular polysaccharide ...

journal_title：Journal of bacteriology

authors： Niemann H,Kwiatkowski B,Westphal U,Stirm S

更新日期：1977-04-01 00:00:00

abstract：:Acyl-homoserine lactone (acyl-HSL) quorum sensing is common to many Proteobacteria including a clinical isolate of Burkholderia cepacia. The B. cepacia isolate produces low levels of octanoyl-HSL. We have examined an environmental isolate of Burkholderia vietnamiensis. This isolate produced several acyl-HSLs. The most...

journal_title：Journal of bacteriology

authors： Conway BA,Greenberg EP

更新日期：2002-02-01 00:00:00

abstract：:The ftf gene, coding for the cell-bound beta-D-fructosyltransferase (FTF) of Streptococcus salivarius ATCC 25975, has been analyzed, and its deduced amino acid sequence has been compared with that of the secreted FTF of Streptococcus mutans and the levansucrases (SacBs) of Bacillus species. A unique proline-rich regio...

journal_title：Journal of bacteriology

authors： Rathsam C,Giffard PM,Jacques NA

更新日期：1993-07-01 00:00:00

abstract：:Cells of Haemophilus influenzae strain Rd competent for genetic transformation irreversibly bound approximately five molecular fragments of H. influenzae deoxyribonucleic acid (DNA) per cell; under identical conditions, DNA derived from Escherichia coli B was not taken up (<1 molecule per 50 cells). Similarly, DNA fro...

journal_title：Journal of bacteriology

authors： Scocca JJ,Poland RL,Zoon KC

更新日期：1974-05-01 00:00:00

abstract：:The chlorosome envelope of Chlorobaculum tepidum contains 10 proteins that belong to four structural motif families. A previous mutational study (N.-U. Frigaard, H. Li, K. J. Milks, and D. A. Bryant, J. Bacteriol. 186:646-653, 2004) suggested that some of these proteins might have redundant functions. Six multilocus m...

journal_title：Journal of bacteriology

authors： Li H,Bryant DA

更新日期：2009-11-01 00:00:00

abstract：:Borrelia burgdorferi is a motile spirochete which has been identified as the causative microorganism in Lyme disease. The physiological functions which govern the motility of this organism have not been elucidated. In this study, we found that motility of B. burgdorferi required an environment similar to interstitial ...

journal_title：Journal of bacteriology

authors： Shi W,Yang Z,Geng Y,Wolinsky LE,Lovett MA

更新日期：1998-01-01 00:00:00

abstract：:Methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila TM-1 was purified 16-fold from a cell extract to apparent homogeneity as determined by native polyacrylamide gel electrophoresis. Ninety-four percent of the methylreductase activity was recovered in the soluble fraction of cell extracts. T...

journal_title：Journal of bacteriology

authors： Jablonski PE,Ferry JG

更新日期：1991-04-01 00:00:00

abstract：:The double-stranded DNA bacteriophage PRD1 uses an IncP plasmid-encoded conjugal transfer complex as a receptor. Plasmid functions in the PRD1 life cycle are restricted to phage adsorption and DNA entry. A single phage structural protein, P2, located at the fivefold capsid vertices, is responsible for PRD1 attachment ...

journal_title：Journal of bacteriology

authors： Grahn AM,Caldentey J,Bamford JK,Bamford DH

更新日期：1999-11-01 00:00:00

abstract：:Lankford, C. E. (The University of Texas, Austin), James R. Walker, James B. Reeves, N. H. Nabbut, B. R. Byers, and R. J. Jones. Inoculum-dependent division lag of Bacillus cultures and its relation to an endogenous factor(s) ("schizokinen"). J. Bacteriol. 91:1070-1079. 1966.-When cells of Bacillus megaterium, grown o...

journal_title：Journal of bacteriology

authors： Lankford CE,Walker JR,Reeves JB,Nabbut NH,Byers BR,Jones RJ

更新日期：1966-03-01 00:00:00

abstract：:Toxin-antitoxin (TA) loci consist of two genes in an operon, encoding a stable toxin and an unstable antitoxin. The expression of toxin leads to cell growth arrest and sometimes bacterial death, while the antitoxin prevents the cytotoxic activity of the toxin. In this study, we show that the chromosome of Yersinia pes...

journal_title：Journal of bacteriology

authors： Goulard C,Langrand S,Carniel E,Chauvaux S

更新日期：2010-07-01 00:00:00

abstract：:E2 (dihydrolipoamide acetyltransferase) and E3 (dihydrolipoamide dehydrogenase) of the Clostridium magnum acetoin dehydrogenase enzyme system were copurified in a three-step procedure from acetoin-grown cells. The denatured E2-E3 preparation comprised two polypeptides with M(r)s of 49,000 and 67,000, respectively. Mic...

journal_title：Journal of bacteriology

authors： Krüger N,Oppermann FB,Lorenzl H,Steinbüchel A

更新日期：1994-06-01 00:00:00

abstract：:We report the complete and annotated genome sequence of the nonpathogenic Listeria seeligeri SLCC3954 serovar 1/2b type strain harboring the smallest completely sequenced genome of the genus Listeria. ...

journal_title：Journal of bacteriology

authors： Steinweg C,Kuenne CT,Billion A,Mraheil MA,Domann E,Ghai R,Barbuddhe SB,Kärst U,Goesmann A,Pühler A,Weisshaar B,Wehland J,Lampidis R,Kreft J,Goebel W,Chakraborty T,Hain T

更新日期：2010-03-01 00:00:00

abstract：:From the marine sponge Halichondria panicea a lectin was isolated and characterized. The homogeneous lectin (composed of protein to 80.7% and of neutral carbohydrates to 14.1%) had a molecular weight of 78,000 (determined by gel filtration) and consisted of four subunits with a molecular weight of 21,000 each (determi...

journal_title：Journal of bacteriology

authors： Müller WE,Zahn RK,Kurelec B,Lucu C,Müller I,Uhlenbruck G

更新日期：1981-01-01 00:00:00

abstract：:Pseudomonas aeruginosa type IV pili (T4P) are virulence factors that promote infection of cystic fibrosis and immunosuppressed patients. As the absence of T4P impairs colonization, they are attractive targets for the development of novel therapeutics. Genes in the pilMNOPQ operon are important for both T4P assembly an...

journal_title：Journal of bacteriology

authors： Tammam S,Sampaleanu LM,Koo J,Manoharan K,Daubaras M,Burrows LL,Howell PL

更新日期：2013-05-01 00:00:00

abstract：:Recipient (F(-)) cells of Escherichia coli are sensitive to an excess of Hfr donor cells. This phenomenon of lethal zygosis is associated with conjugation and is observed as a continuous fall in F(-) viable cells during liquid mating, or as inhibition of F(-) growth on solid media. One class of survivors, which arose ...

journal_title：Journal of bacteriology

authors： Skurray RA,Reeves P

更新日期：1974-01-01 00:00:00

abstract：:Spontaneous, erythromycin-resistant mutants of Thermus thermophilus IB-21 were isolated and found to carry the mutation A2058G in one of two 23S rRNA operons. The heterozygosity of these mutants indicates that A2058G confers a dominant or codominant phenotype in this organism. This mutation provides a valuable tool fo...

journal_title：Journal of bacteriology

authors： Gregory ST,Cate JH,Dahlberg AE

更新日期：2001-07-01 00:00:00

abstract：:A method for polyethylene glycol-induced protoplast transformation of glutamate-producing bacteria with plasmid DNA was established. Protoplasts were prepared from cells grown in the presence of penicillin by treatment with lysozyme in a hypertonic medium. The concentration of penicillin during growth affected the eff...

journal_title：Journal of bacteriology

authors： Katsumata R,Ozaki A,Oka T,Furuya A

更新日期：1984-07-01 00:00:00

abstract：:Spores of all strains of Dictyostelium discoideum tested in this study germinated after a heat shock of 45 C for 30 min. Whereas the strains differed in their rates of germination, the rate for each strain was constant. A correlation existed between the rate of germination and the rate of vegetative growth when spores...

journal_title：Journal of bacteriology

authors： Cotter DA,Raper KB

更新日期：1968-11-01 00:00:00

abstract：:The gene encoding the general stress transcription factor sigmaB in the gram-positive bacterium Listeria monocytogenes was isolated with degenerate PCR primers followed by inverse PCR amplification. Evidence for gene identification includes the following: (i) phylogenetic analyses of reported amino acid sequences for ...

journal_title：Journal of bacteriology

authors： Wiedmann M,Arvik TJ,Hurley RJ,Boor KJ

更新日期：1998-07-01 00:00:00

abstract：:Genome sequencing revealed that all six chlamydiae genomes contain three groEL-like genes (groEL1, groEL2, and groEL3). Phylogenetic analysis of groEL1, groEL2, and groEL3 indicates that these genes are likely to have been present in chlamydiae since the beginning of the lineage. Comparison of deduced amino acid seque...

journal_title：Journal of bacteriology

authors： Karunakaran KP,Noguchi Y,Read TD,Cherkasov A,Kwee J,Shen C,Nelson CC,Brunham RC

更新日期：2003-03-01 00:00:00