Abstract:
:In Staphylococcus aureus, the transport of dicarboxylates is mediated in part by the Na+-linked carrier protein SdcS. This transporter is a member of the divalent-anion/Na+ symporter (DASS) family, a group that includes the mammalian Na+/dicarboxylate cotransporters NaDC1 and NaDC3. In earlier work, we cloned and expressed SdcS in Escherichia coli and found it to have transport properties similar to those of its eukaryotic counterparts (J. A. Hall and A. M. Pajor, J. Bacteriol. 187:5189-5194, 2005). Here, we report the partial purification and subsequent reconstitution of functional SdcS into liposomes. These proteoliposomes exhibited succinate counterflow activity, as well as Na+ electrochemical-gradient-driven transport. Examination of substrate specificity indicated that the minimal requirement necessary for transport was a four-carbon terminal dicarboxylate backbone and that productive substrate-transporter interaction was sensitive to substitutions at the substrate C-2 and C-3 positions. Further analysis established that SdcS facilitates an electroneutral symport reaction having a 2:1 cation/dicarboxylate ratio. This study represents the first characterization of a reconstituted Na+-coupled DASS family member, thus providing an effective method to evaluate functional, as well as structural, aspects of DASS transporters in a system free of the complexities and constraints associated with native membrane environments.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hall JA,Pajor AMdoi
10.1128/JB.01452-06subject
Has Abstractpub_date
2007-02-01 00:00:00pages
880-5issue
3eissn
0021-9193issn
1098-5530pii
JB.01452-06journal_volume
189pub_type
杂志文章abstract::Escherichia coli K-12 produces at least two ATP-dependent proteases, Lon (La) and Clp (Ti), the latter consisting of a regulatory subunit (ClpA) and a proteolytic subunit (ClpP). The gene clpB encoding an analog of ClpA had been found at 57 min on the E. coli chromosome. Cloning and examination of novel heat shock pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.14.4247-4253.1991
更新日期:1991-07-01 00:00:00
abstract::Chlorate-resistant mutants corresponding to each known genetic locus (chlA, chlB, chlC, chlD, chlE) were isolated from Escherichia coli K-12. All these mutants showed decreased amounts of membrane-bound nitrate reductase, cytochrome b, and formic dehydrogenase, but all had normal succinic dehydrogenase activity. Prote...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.1.564-570.1971
更新日期:1971-10-01 00:00:00
abstract::The protein motive force of metabolizing Bacillus subtilis cells was only slightly affected by changes in the external pH between 5 and 8, although the electrical component and the chemical component of the proton motive force contributed differently at different external pH. The electrical component of the proton mot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.3.891-897.1980
更新日期:1980-12-01 00:00:00
abstract::The kinetics of chromosome replication and cell division have been examined in recA mutants of Escherichia coli B/r containing F' plasmids of various sizes. Plasmid-mediated alterations in growth properties were detected only with the presence of the larger F' plasmids, and were reflected in decreased mean cell sizes ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.3.1151-1157.1979
更新日期:1979-03-01 00:00:00
abstract::The peptidoglycan cell wall determines the shape and structural integrity of a bacterial cell. Class B penicillin-binding proteins (PBPs) carry a transpeptidase activity that cross-links peptidoglycan strands via their peptide side chains, and some of these proteins are directly involved in cell shape determination. N...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.16.4717-4726.2003
更新日期:2003-08-01 00:00:00
abstract::The opportunistic pathogen Pseudomonas aeruginosa utilizes a type III secretion system (T3SS) to evade phagocytosis and damage eukaryotic cells. Transcription of the T3SS regulon is controlled by ExsA, a member of the AraC/XylS family of transcriptional regulators. These family members generally consist of an approxim...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00002-09
更新日期:2009-06-01 00:00:00
abstract::The Bacillus subtilis ferric uptake regulator (Fur) protein is the major sensor of cellular iron status. When iron is limiting for growth, derepression of the Fur regulon increases the cellular capacity for iron uptake and mobilizes an iron-sparing response mediated in large part by a small noncoding RNA named FsrA. F...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05990-11
更新日期:2012-05-01 00:00:00
abstract::Four chloramphenicol resistance (Cm) and four tetracycline resistance (Tc) plasmids from Staphylococcus aureus were characterized by restriction endonuclease mapping. All four Tc plasmids had molecular masses of 2.9 megadaltons (Mdaltons) and indistinguishable responses to seven different restriction endonucleases. Th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.1.402-413.1978
更新日期:1978-10-01 00:00:00
abstract::The role of the proton gradient and external pH in the motility and chemotaxis of Bacillus subtilis was investigated. Presence of a substantial proton gradient is not necessary for motility or chemotaxis, as long as the electrical potential is sufficient to maintain motility. Changes in the proton gradient do, however...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.1.26-32.1980
更新日期:1980-01-01 00:00:00
abstract::The coding region of the alpha-amylase inhibitor (HaimII) gene from the producing strain Streptomyces griseosporeus YM-25 was localized on an 800-base-pair DNA segment. The nucleotide sequence of a 1,191-base-pair region including the HaimII gene was determined by the dideoxy-chain termination method. The nucleotide s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4451-4457.1988
更新日期:1988-10-01 00:00:00
abstract::A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.3.854-859.1984
更新日期:1984-12-01 00:00:00
abstract::The distribution of a pulse of teichoic acid-specific radiolabel between wall and membrane teichoic acids in pneumococci was constant over a subsequent chase period, suggesting that wall and membrane teichoic acids are biosynthesized simultaneously and independently. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.1.335-337.1975
更新日期:1975-04-01 00:00:00
abstract::Berry, L. Joe (Bryn Mawr College, Bryn Mawr, Pa.), Dorothy S. Smythe, and Louise S. Colwell. Inhibition of inducible liver enzymes by endotoxin and actinomycin D. J. Bacteriol. 92:107-115. 1966.-Bacterial endotoxin at the ld(50) level lowers liver tryptophan pyrrolase in mice, it prevents for 16 to 20 hr the induction...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.1.107-115.1966
更新日期:1966-07-01 00:00:00
abstract::Site-directed mutagenesis has previously been used to construct Escherichia coli dnaK mutants encoding proteins that are altered at the site of in vitro phosphorylation (J. S. McCarty and G. C. Walker, Proc. Natl. Acad. Sci. USA 88:9513-9517, 1991). These mutants are unable to autophosphorylate and are severely defect...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.3.764-780.1994
更新日期:1994-02-01 00:00:00
abstract::Shiio, Tsuru (Washington State University, Pullman), and Bruce A. McFadden. Cell-free amino acid-incorporating system from Pseudomonas indigofera. J. Bacteriol. 90:978-983. 1965.-A cell-free preparation from Pseudomonas indigofera incorporated C(14)-phenylalanine and C(14)-leucine into a product which was insoluble in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.4.978-983.1965
更新日期:1965-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.10.3178-3184.1999
更新日期:1999-05-01 00:00:00
abstract::The O antigen of the Shigella flexneri lipopolysaccharide (LPS) is an important virulence determinant and immunogen. We have isolated S. flexneri mutants which produce a semi-rough LPS by using an O-antigen-specific phage, Sf6c. Western immunoblotting was used to show that the LPS produced by the semi-rough mutants co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.3.733-747.1994
更新日期:1994-02-01 00:00:00
abstract::Cyanothece sp. strain ATCC 51142 is a unicellular, diazotrophic cyanobacterium which demonstrated extensive metabolic periodicities of photosynthesis, respiration, and nitrogen fixation when grown under N2-fixing conditions. N2 fixation and respiration peaked at 24-h intervals early in the dark or subjective-dark peri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.13.4319-4327.1997
更新日期:1997-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.2.527-537.1971
更新日期:1971-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.2.640-645.1970
更新日期:1970-11-01 00:00:00
abstract::The first step in the transfer of the Bacteroides conjugative transposon CTnDOT is excision of the integrated element from the chromosome to form a circular transfer intermediate. Excision occurs only after the bacteria are exposed to tetracycline. Previously, four excision genes were identified. One was the integrase...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.16.5732-5741.2005
更新日期:2005-08-01 00:00:00
abstract::Escherichia coli outer membrane protein E was purified, and its amino acid composition and N-terminal amino acid were determined. The purified protein was shown to be immunologically and electrophoretically identical to proteins Ic (U. Henning, W. Schmidmayr, and I. Hindennach, Mol. Gen. Genet. 154:293-298, 1977) and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.418-423.1979
更新日期:1979-08-01 00:00:00
abstract::Thin pili of the closely related IncI1 plasmids ColIb-P9 and R64 are required only for liquid mating and belong to the type IV family of pili. They were sedimented by ultracentrifugation from culture medium in which Escherichia coli cells harboring ColIb-P9- or R64-derived plasmids had been grown, and then the pili we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.11.2842-2848.1998
更新日期:1998-06-01 00:00:00
abstract::Band 7 proteins, which encompass members of the stomatin, prohibitin, flotillin, and HflK/C protein families, are integral membrane proteins that play important physiological roles in eukaryotes but are poorly characterized in bacteria. We have studied the band 7 proteins encoded by the cyanobacterium Synechocystis sp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00644-09
更新日期:2009-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.20.6015-6017.1995
更新日期:1995-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.2.815-817.1978
更新日期:1978-11-01 00:00:00
abstract::In rod-shaped bacteria, certain proteins are specifically localized to the cell poles. The nature of the positional information that leads to the proper localization of these proteins is unclear. In a screen for factors required for the localization of the Shigella sp. actin assembly protein IcsA to the bacterial pole...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.17.6187-6196.2005
更新日期:2005-09-01 00:00:00
abstract::Shapira, Jacob (Consolidated Veterans Administration Hospital, Little Rock, Ark.), Lois Lowden, and Ralph Hale. Inhibition of a thymine-deficient mutant of Escherichia coli by 5-substituted uracils. J. Bacteriol. 83:919-923. 1962.-Small inocula of well-washed cells of a thymine-requiring mutant of Escherichia coli wer...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.4.919-923.1962
更新日期:1962-04-01 00:00:00
abstract::Growth of Lactococcus lactis in milk depends on the utilization of extracellular peptides. Up to now, oligopeptide uptake was thought to be due only to the ABC transporter Opp. Nevertheless, analysis of several Opp-deficient L. lactis strains revealed the implication of a second oligopeptide ABC transporter, the so-ca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.19.6492-6500.2004
更新日期:2004-10-01 00:00:00
abstract::The Staphylococcus aureus autoinducer-2 (AI-2) producer protein LuxS is phosphorylated by the Ser/Thr kinase Stk1 at a unique position, Thr14. The enzymatic activity of the phosphorylated isoform of LuxS was abrogated compared to that of nonphosphorylated LuxS, thus providing the first evidence of an AI-2-producing en...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00853-10
更新日期:2010-12-01 00:00:00