Characterization and construction of molecular cloning vehicles within Staphylococcus aureus.

abstract：:Agrobacterium tumefaciens and Agrobacterium rhizogenes are related pathogens that cause crown gall and hairy root diseases, which result from integration and expression of bacterial genes in the plant genome. Single-stranded DNA (T strands) and virulence proteins are translocated into plant cells by a type IV secretio...

journal_title：Journal of bacteriology

authors： Hodges LD,Lee LY,McNett H,Gelvin SB,Ream W

更新日期：2009-01-01 00:00:00

abstract：:The development of competence by the dental caries pathogen Streptococcus mutans is mediated primarily through the alternative sigma factor ComX (SigX), which is under the control of multiple regulatory systems and activates the expression of genes involved in DNA uptake and recombination. Here we report that the indu...

journal_title：Journal of bacteriology

authors： Guo Q,Ahn SJ,Kaspar J,Zhou X,Burne RA

更新日期：2014-01-01 00:00:00

abstract：:Inositol derivative compounds provide a nutrient source for soil bacteria that possess the ability to degrade such compounds. Rhizobium strains that are capable of utilizing certain inositol derivatives are better colonizers of their host plants. We have cloned and determined the nucleotide sequence of the myo-inosito...

journal_title：Journal of bacteriology

authors： Jiang G,Krishnan AH,Kim YW,Wacek TJ,Krishnan HB

更新日期：2001-04-01 00:00:00

abstract：:Clostridium perfringens is an anaerobic Gram-positive pathogen that causes many human and animal diseases, including food poisoning and gas gangrene. C. perfringens lacks flagella but possesses type IV pili (TFP). We have previously shown that C. perfringens can glide across an agar surface in long filaments composed ...

journal_title：Journal of bacteriology

authors： Liu H,Bouillaut L,Sonenshein AL,Melville SB

更新日期：2013-02-01 00:00:00

abstract：:The production of extracellular enzymes such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt) is activated by the cell density (quorum)-sensing signal, N-(3-oxohexanoyl)-L-homoserine lactone (HSL); plant signals; and aep genes during postexponential growth of Erwinia carotovora subs...

journal_title：Journal of bacteriology

authors： Cui Y,Chatterjee A,Liu Y,Dumenyo CK,Chatterjee AK

更新日期：1995-09-01 00:00:00

abstract：:DNA-binding assays using mobility shift polyacrylamide gel electrophoresis revealed the presence of a protein that specifically bound to a restriction fragment -288 to -191 bp upstream from the transcriptional start point of strR, a regulatory gene for streptomycin biosynthesis in Streptomyces griseus. The binding sit...

journal_title：Journal of bacteriology

authors： Vujaklija D,Horinouchi S,Beppu T

更新日期：1993-05-01 00:00:00

abstract：:The herpes simplex virus thymidine kinase gene has been cloned into a chimeric yeast plasmid cloning vehicle and transformed into appropriate yeast strains. Plasmids carrying the herpes simplex virus thymidine kinase gene can be propagated as autonomously replicating plasmids, but no RNA specific to the thymidine kina...

journal_title：Journal of bacteriology

authors： Kiss GB,Pearlman RE,Cornish KV,Friesen JD,Chan VL

更新日期：1982-02-01 00:00:00

abstract：:Cellular vanadium metabolism was studied in Saccharomyces cerevisiae by isolating and characterizing vanadate [VO4(3-), V(V)]-resistant mutants. Vanadate growth inhibition was reversed by the removal of the vanadate from the medium, and vanadate resistance was found to be a recessive trait. Vanadate-resistant mutants ...

journal_title：Journal of bacteriology

authors： Willsky GR,Leung JO,Offermann PV Jr,Plotnick EK,Dosch SF

更新日期：1985-11-01 00:00:00

abstract：:The ability of deoxyribonucleic acid (DNA) isolated from a number of actinomycetes to reassociate with reference DNA from Actinoplanes philippinensis or Streptomyces venezuelae has been measured. All of the DNA preparations except for those from Nocardia erythropolis and Thermomonospora viridis contained 70 to 73 mole...

journal_title：Journal of bacteriology

authors： Farina G,Bradley SG

更新日期：1970-04-01 00:00:00

abstract：:In vitro, archaeal SRP54 binds SRP RNA in the absence of SRP19, suggesting the latter to be expendable in Archaea. Accordingly, the Haloferax volcanii SRP19 gene was deleted. Although normally transcribed at a level comparable to that of the essential SRP54 gene, SRP19 deletion had no effect on cell growth, membrane p...

journal_title：Journal of bacteriology

authors： Yurist S,Dahan I,Eichler J

更新日期：2007-01-01 00:00:00

abstract：:The nucleotide sequence of the Clostridium thermocellum F1 xynC gene, which encodes the xylanase XynC, consists of 1,857 bp and encodes a protein of 619 amino acids with a molecular weight of 69,517. XynC contains a typical N-terminal signal peptide of 32 amino acid residues, followed by a 165-amino-acid sequence whic...

journal_title：Journal of bacteriology

authors： Hayashi H,Takagi KI,Fukumura M,Kimura T,Karita S,Sakka K,Ohmiya K

更新日期：1997-07-01 00:00:00

abstract：:MarR is a key regulator of the marRAB operon involved in antibiotic resistance and solvent stress tolerance in Escherichia coli. We show that two metabolic intermediates, 2,3-dihydroxybenzoate and anthranilate, involved in enterobactin and tryptophan biosynthesis, respectively, can activate marRAB transcription. We al...

journal_title：Journal of bacteriology

authors： Chubiz LM,Rao CV

更新日期：2010-09-01 00:00:00

abstract：:Eubacterium sp. strain VPI 12708 is an anaerobic intestinal bacterium that has inducible bile acid 7-dehydroxylation activity. At least four new polypeptides were synthesized after addition of primary bile acids to the growth medium. One of these, of molecular weight 27,000 (P-27), was shown to be involved in the 7-de...

journal_title：Journal of bacteriology

authors： Coleman JP,White WB,Lijewski M,Hylemon PB

更新日期：1988-05-01 00:00:00

abstract：:Protein phosphorylation was demonstrated in Bradyrhizobium japonicum bacteroids in vivo and in cultures in vivo and in vitro. Comparison of in vivo-labeled phosphoproteins of bacteroids and of cultured cells showed differences in both the pattern and intensity of labeling. In cultured cells, comparison of the labeling...

journal_title：Journal of bacteriology

authors： Karr DB,Emerich DW

更新日期：1989-06-01 00:00:00

abstract：:It has been shown previously that Bacteroides thetaiotaomicron, a human colonic anaerobe, can utilize the tissue mucopolysaccharide chondroitin sulfate as a source of carbon and energy and that the enzymes involved in this utilization are all cell associated (A. A. Salyers and M. B. O'Brien, J. Bacteriol. 143:772-780,...

journal_title：Journal of bacteriology

authors： Kuritza AP,Salyers AA

更新日期：1983-03-01 00:00:00

abstract：:Western immunoblots and assays of Bacillus subtilis extracts showed that intracellular serine protease 1 is produced in a form larger than previously reported, appears not to have undergone N-terminal processing, and is active in the presence or absence of calcium. No evidence for an inactive precursor form of the pro...

journal_title：Journal of bacteriology

authors： Sheehan SM,Switzer RL

更新日期：1990-01-01 00:00:00

abstract：:Type III machines of pathogenic Yersinia spp. transport Yop proteins across the bacterial envelope into host cells. Translational fusions of yopE to the dihydrofolate reductase gene (dhfr) or the beta-galactosidase gene (lacZ) generate hybrid proteins that block type III injection of Yop proteins into host cells, cons...

journal_title：Journal of bacteriology

authors： Sorg JA,Miller NC,Marketon MM,Schneewind O

更新日期：2005-10-01 00:00:00

abstract：:Procedures for the purification of catechol 1,2-dioxygenase from extracts of Acinetobacter calcoaceticus strain ADP-96 are described. The purified enzyme was homogeneous as judged by ultracentrifugation and acrylamide gel electrophoresis. The enzyme contained 2 g-atoms of iron per mol of protein. The enzyme had a broa...

journal_title：Journal of bacteriology

authors： Patel RN,Hou CT,Felix A,Lillard MO

更新日期：1976-07-01 00:00:00

abstract：:Congo red binding by virulent A-layer-containing (A+) and avirulent A-layer-deficient (A-) strains of Aeromonas salmonicida was examined. Congo red binding to A+ cells was enhanced by salt and thus hydrophobically driven, but at low Congo red concentrations binding was salt independent. Congo red was bound by A+ cells...

journal_title：Journal of bacteriology

authors： Kay WW,Phipps BM,Ishiguro EE,Trust TJ

更新日期：1985-12-01 00:00:00

abstract：:The pink yeast Rhodotorula rubra of marine origin was found to be capable of extended growth at very low phosphate concentrations (K(0.5) = 10.8 nm). Average intracellular phosphate concentrations, based on isotope exchange techniques, were 15 to 200 nm, giving concentration gradients across the cell envelope of about...

journal_title：Journal of bacteriology

authors： Button DK,Dunker SS,Morse ML

更新日期：1973-02-01 00:00:00

abstract：:YoeB is a bacterial toxin encoded by the yefM-yoeB toxin-antitoxin system found in various bacterial genomes. Here, we show that Staphylococcus aureus contains two YoeB homologues, both of which function as ribosome-dependent mRNA interferases to inhibit translation initiation in a manner identical to that of YoeB-ec ...

journal_title：Journal of bacteriology

authors： Yoshizumi S,Zhang Y,Yamaguchi Y,Chen L,Kreiswirth BN,Inouye M

更新日期：2009-09-01 00:00:00

abstract：:The expression of the positively charged human protein secretory leukocyte protease inhibitor (SLPI) in Escherichia coli causes severe cellular toxicity. After induction of SLPI synthesis in a high-level-expression strain, SGE61, the growth of the strain is arrested and total protein and RNA synthesis rates decline by...

journal_title：Journal of bacteriology

authors： Miller KW,Evans RJ,Eisenberg SP,Thompson RC

更新日期：1989-04-01 00:00:00

abstract：:A strain of Escherichia coli which was derived from a gentamicin-resistant clinical isolate was found to be cross-resistant to neomycin and streptomycin. The molecular nature of the genetic defect was found to be an insertion of two GC base pairs in the uncG gene of the mutant. The insertion led to the production of a...

journal_title：Journal of bacteriology

authors： Humbert R,Altendorf K

更新日期：1989-03-01 00:00:00

abstract：:Escherichia coli acetyl coenzyme A carboxylase (ACC), the first enzyme of the fatty acid biosynthetic pathway, is inhibited by acylated derivatives of acyl carrier protein (ACP). ACP lacking an acyl moiety does not inhibit ACC. Acylated derivatives of ACP having chain lengths of 6 to 20 carbon atoms were similarly inh...

journal_title：Journal of bacteriology

authors： Davis MS,Cronan JE Jr

更新日期：2001-02-01 00:00:00

abstract：:A lambda phage (lambdaNK55) carrying the translocatable element Tn10, conferring tetracycline resistance (Tetr), has been utilized to isolate glutamine auxotrophs of Escherichia coli K-12. Such strains lack uridylyltransferase as a result of an insertion of the TN10 element in the glnD gene. The glnD::Tn10 insertion h...

journal_title：Journal of bacteriology

authors： Bloom FR,Levin MS,Foor F,Tyler B

更新日期：1978-05-01 00:00:00

abstract：:Short sequence repeats (SSRs), potentially representing variable numbers of tandem repeat (VNTR) loci, were identified for the human-pathogenic yeast species Candida albicans by computerized DNA sequence scanning. The individual SSR regions were investigated in different clinical isolates of C. albicans. Most of the C...

journal_title：Journal of bacteriology

authors： Lunel FV,Licciardello L,Stefani S,Verbrugh HA,Melchers WJ,Meis JF,Scherer S,van Belkum A

更新日期：1998-08-01 00:00:00

abstract：:A thermosensitive sporulation mutant of Bacillus subtilis containing a mutation in the secY gene was isolated and characterized. No asymmetric septum specific to the sporulation was detected by electron microscopy at the nonpermissive temperature, indicating that the block occurred at a very early stage of sporulation...

journal_title：Journal of bacteriology

authors： Yoshikawa H,Jeong SM,Hirata A,Kawamura F,Doi RH,Takahashi H

更新日期：1993-06-01 00:00:00

abstract：:We have identified an analog of SspB, the proteolytic adaptor for transfer-messenger RNA (tmRNA)-tagged proteins, in Caulobacter crescentus. C. crescentus SspB shares limited sequence similarity with Escherichia coli SspB but binds the tmRNA tag in vitro and is required for optimal proteolysis of tagged proteins in vi...

journal_title：Journal of bacteriology

authors： Lessner FH,Venters BJ,Keiler KC

更新日期：2007-01-01 00:00:00

abstract：:Germination of Bacillus subtilis spores is normally initiated when nutrients from the environment interact with germinant receptors (GRs) in the spores' inner membrane (IM), in which most of the lipids are immobile. GRs and another germination protein, GerD, colocalize in the IM of dormant spores in a small focus term...

journal_title：Journal of bacteriology

authors： Troiano AJ Jr,Zhang J,Cowan AE,Yu J,Setlow P

更新日期：2015-01-01 00:00:00

abstract：:A primary determinant of pathogenicity in Erwinia stewartii is the production of extracellular polysaccharide (EPS). A single mutation can abolish both EPS synthesis and pathogenicity; both properties are restored by a single cosmid clone. Subcloning and insertion analysis have defined a single positive regulatory fun...

journal_title：Journal of bacteriology

authors： Torres-Cabassa A,Gottesman S,Frederick RD,Dolph PJ,Coplin DL

更新日期：1987-10-01 00:00:00