Abstract:
:Type III machines of pathogenic Yersinia spp. transport Yop proteins across the bacterial envelope into host cells. Translational fusions of yopE to the dihydrofolate reductase gene (dhfr) or the beta-galactosidase gene (lacZ) generate hybrid proteins that block type III injection of Yop proteins into host cells, consistent with the canonical view that impassable DHFR and LacZ hybrids jam secretion machines. Mutations in repressors of posttranscriptional gene regulation, Yersinia enterocolitica yscM1 and yscM2 as well as Yersinia pestis lcrQ, relieve the YopE-DHFR-imposed blockade and restore type III injection into host cells. Genetic suppression of the type III blockade does not, however, promote YopE-DHFR secretion. A model is proposed whereby rejection of YopE-DHFR from the secretion pathway inhibits type III gene expression.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Sorg JA,Miller NC,Marketon MM,Schneewind Odoi
10.1128/JB.187.20.7090-7102.2005keywords:
subject
Has Abstractpub_date
2005-10-01 00:00:00pages
7090-102issue
20eissn
0021-9193issn
1098-5530pii
187/20/7090journal_volume
187pub_type
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1969-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1975-05-01 00:00:00