Characterization of the Synechocystis strain PCC 6803 penicillin-binding proteins and cytokinetic proteins FtsQ and FtsW and their network of interactions with ZipN.

Abstract:

:Because very little is known about cell division in noncylindrical bacteria and cyanobacteria, we investigated 10 putative cytokinetic proteins in the unicellular spherical cyanobacterium Synechocystis strain PCC 6803. Concerning the eight penicillin-binding proteins (PBPs), which define three classes, we found that Synechocystis can survive in the absence of one but not two PBPs of either class A or class C, whereas the unique class B PBP (also termed FtsI) is indispensable. Furthermore, we showed that all three classes of PBPs are required for normal cell size. Similarly, the putative FtsQ and FtsW proteins appeared to be required for viability and normal cell size. We also used a suitable bacterial two-hybrid system to characterize the interaction web among the eight PBPs, FtsQ, and FtsW, as well as ZipN, the crucial FtsZ partner that occurs only in cyanobacteria and plant chloroplasts. We showed that FtsI, FtsQ, and ZipN are self-interacting proteins and that both FtsI and FtsQ interact with class A PBPs, as well as with ZipN. Collectively, these findings indicate that ZipN, in interacting with FtsZ and both FtsI and FtQ, plays a similar role to the Escherichia coli FtsA protein, which is missing in cyanobacteria and chloroplasts.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Marbouty M,Mazouni K,Saguez C,Cassier-Chauvat C,Chauvat F

doi

10.1128/JB.00620-09

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

5123-33

issue

16

eissn

0021-9193

issn

1098-5530

pii

JB.00620-09

journal_volume

191

pub_type

杂志文章
  • Microtubular structures in a stable staphylococcal L-form.

    abstract::Microtubular structures, which were demonstrated as straight, dense-walled cylinders attached to cell membranes, were found in a stable staphylococcal L-form grown in the absence of antibiotic. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.3.1024-1026.1977

    authors: Eda T,Kanda Y,Mori C,Kimura S

    更新日期:1977-12-01 00:00:00

  • The mere lack of rT modification in initiator tRNA does not facilitate formylation-independent initiation in Escherichia coli.

    abstract::Formylation of initiator methionyl-tRNA is essential for normal growth of eubacteria. However, under special conditions, it has been possible to initiate protein synthesis with unformylated initiator tRNA even in eubacteria. Earlier studies suggested that the lack of ribothymidine (rT) modification in initiator tRNA m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.24.7397-7402.2001

    authors: Thanedar S,Dineshkumar TK,Varshney U

    更新日期:2001-12-01 00:00:00

  • Localization of P42 and F(1)-ATPase α-subunit homolog of the gliding machinery in Mycoplasma mobile revealed by newly developed gene manipulation and fluorescent protein tagging.

    abstract::Mycoplasma mobile has a unique mechanism that enables it to glide on solid surfaces faster than any other gliding mycoplasma. To elucidate the gliding mechanism, we developed a transformation system for M. mobile based on a transposon derived from Tn4001. Modification of the electroporation conditions, outgrowth time,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01418-13

    authors: Tulum I,Yabe M,Uenoyama A,Miyata M

    更新日期:2014-05-01 00:00:00

  • Bacterial plasmids that carry two functional centromere analogs are stable and are partitioned faithfully.

    abstract::The par genes of unit-copy plasmids P1 and F promote equitable distribution of plasmid copies to daughter cells and can be considered to be functional analogs of eucaryotic centromeres. Composite plasmids were constructed which carry either two functional P1 par regions or one F and one P1 region. Unlike dicentric chr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.2.742-745.1984

    authors: Austin SJ

    更新日期:1984-05-01 00:00:00

  • Characterization of the regulatory region of a cell interaction-dependent gene in Myxococcus xanthus.

    abstract::omega 4403 is the site of a Tn5 lac insertion in the Myxococcus xanthus genome that fuses lacZ expression to a developmentally regulated promoter. Cell-cell interactions that occur during development, including C-signaling, are required for expression of Tn5 lac omega 4403. We have cloned DNA upstream of the omega 440...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.9.2539-2550.1996

    authors: Fisseha M,Gloudemans M,Gill RE,Kroos L

    更新日期:1996-05-01 00:00:00

  • Characterization of a gene cluster for exopolysaccharide biosynthesis and virulence in Erwinia stewartii.

    abstract::We have previously cloned the genes for synthesis of capsular polysaccharide (cps) and slime from Erwinia stewartii in cosmid pES2144. In this study, pES2144 was shown to complement 14 spontaneous cps mutants. These mutants were characterized by probing Southern blots of mutant genomic DNA with pES2144; insertions wer...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.2.865-871.1988

    authors: Dolph PJ,Majerczak DR,Coplin DL

    更新日期:1988-02-01 00:00:00

  • ISOLATION OF TOXIGENIC STRAINS OF CLOSTRIDIUM NOVYI FROM SOIL.

    abstract::Nishida, S. (Kanazawa University, Kanazawa, Japan), and G. Nakagawara. Isolation of toxigenic strains of Clostridium novyi from soil. J. Bacteriol. 88:1636-1640. 1964.-The cultural conditions for toxin production by Clostridium novyi were investigated, and the optimal constituents of a proper medium were determined. A...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.6.1636-1640.1964

    authors: NISHIDA S,NAKAGAWARA G

    更新日期:1964-12-01 00:00:00

  • Phosphorylation and functional properties of the IIA domain of the lactose transport protein of Streptococcus thermophilus.

    abstract::The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a carboxyl-terminal regulatory domain (IIALacS) that is homologous to a family of proteins and protein domains of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) in various organisms, of which IIAGlc of Escherichia coli is the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.2.632-641.1999

    authors: Gunnewijk MG,Postma PW,Poolman B

    更新日期:1999-01-01 00:00:00

  • Mutations to spectinomycin resistance which alleviate the restriction of an amber suppressor by streptomycin resistance.

    abstract::Unlike mutations to streptomycin resistance, which restrict the function of an SU II amber suppressor, mutations to spectinomycin resistance have no restrictive effect. Furthermore, mutations to spectinomycin resistance counteract the restrictive action of streptomycin-resistant mutations when combined in a single str...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.2.940-943.1969

    authors: Kuwano M,Endo H,Ohnishi Y

    更新日期:1969-02-01 00:00:00

  • Anaerobiosis induces expression of ant, a new Escherichia coli locus with a role in anaerobic electron transport.

    abstract::Escherichia coli has a formate hydrogenlyase system which allows it to maintain an electron balance during anaerobic growth by passing electrons from formate to H+ ions, thus generating H2. The Mu d1(Ap lac) bacteriophage was used to generate mutants that were defective in passing electrons from formate to benzyl viol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.1.180-186.1984

    authors: Yerkes JH,Casson LP,Honkanen AK,Walker GC

    更新日期:1984-04-01 00:00:00

  • Lactate metabolism by Veillonella parvula.

    abstract::A strain of Veillonella parvula M4, which grows readily in lactate broth without a requirement for carbon dioxide, has been isolated from the oral cavity. Anaerobic, washed cells of this organism fermented sodium lactate to the following products (moles/100 moles of lactate): propionate, 66; acetate, 40; carbon dioxid...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.3.999-1005.1971

    authors: Ng SK,Hamilton IR

    更新日期:1971-03-01 00:00:00

  • Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins.

    abstract::The gene that encodes the acyl carrier protein (ACP) of the actinorhodin polyketide synthase (PKS) of Streptomyces coelicolor A3(2) was replaced with homologs from the granaticin, oxytetracycline, tetracenomycin, and putative frenolicin polyketide synthase gene clusters. All of the replacements led to expression of fu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.8.2197-2204.1993

    authors: Khosla C,McDaniel R,Ebert-Khosla S,Torres R,Sherman DH,Bibb MJ,Hopwood DA

    更新日期:1993-04-01 00:00:00

  • Glycerol metabolism and PrfA activity in Listeria monocytogenes.

    abstract::Listeria monocytogenes is able to efficiently utilize glycerol as a carbon source. In a defined minimal medium, the growth rate (during balanced growth) in the presence of glycerol is similar to that in the presence of glucose or cellobiose. Comparative transcriptome analyses of L. monocytogenes showed high-level tran...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00259-08

    authors: Joseph B,Mertins S,Stoll R,Schär J,Umesha KR,Luo Q,Müller-Altrock S,Goebel W

    更新日期:2008-08-01 00:00:00

  • Structure, function, and regulation of the kilB locus of promiscuous plasmid RK2.

    abstract::The kil-kor regulon of the self-transmissible, broad-host-range plasmid RK2 is a unique network with eight coregulated operons. Among the genes encoded by the kil-kor regulon are trfA, which encodes the replication initiator, and several kil loci (kilA, kilB, kilC, and kilE), each of which is lethal to the host cell i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.8.2423-2435.1993

    authors: Thomson VJ,Jovanovic OS,Pohlman RF,Chang CH,Figurski DH

    更新日期:1993-04-01 00:00:00

  • Coupling of asymmetric division to polar placement of replication origin regions in Bacillus subtilis.

    abstract::Entry into sporulation in Bacillus subtilis is characterized by the formation of a polar septum, which asymmetrically divides the developing cell into forespore (the smaller cell) and mother cell compartments, and by migration of replication origin regions to extreme opposite poles of the cell. Here we show that polar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.13.4052-4060.2001

    authors: Graumann PL,Losick R

    更新日期:2001-07-01 00:00:00

  • Polyamine transport and role of potE in response to osmotic stress in Escherichia coli.

    abstract::When transport of polyamines in Escherichia coli was examined, putrescine excretion was observed under two different physiological conditions: (i) strictly correlated to growth and (ii) following a hyperosmotic shock. Spermidine was not excreted. Characterization of a deletion mutant showed that PotE is not involved i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.21.6247-6249.2000

    authors: Schiller D,Kruse D,Kneifel H,Krämer R,Burkovski A

    更新日期:2000-11-01 00:00:00

  • Transport of diamines by Enterococcus faecalis is mediated by an agmatine-putrescine antiporter.

    abstract::Enterococcus faecalis ATCC 11700 is able to use arginine and the diamine agmatine as a sole energy source. Via the highly homologous deiminase pathways, arginine and agmatine are converted into CO2, NH3, and the end products ornithine and putrescine, respectively. In the arginine deiminase pathway, uptake of arginine ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.10.4522-4527.1988

    authors: Driessen AJ,Smid EJ,Konings WN

    更新日期:1988-10-01 00:00:00

  • "Position effect" on dominance in the D-serine deaminase system of Escherichia coli K-12.

    abstract::A completely stable F32 merodiploid has been isolated, which has the dsd genotype dsdA(+)dsdCx3 /F -dsdA(+)dsdC(+). The stabilization apparently does not result from an alteration in the F, or from its integration in the chromosome, but rather from a chromosomal aberration. The stable strain can exist in two forms, wh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.6.1989-1993.1967

    authors: McFall E

    更新日期:1967-12-01 00:00:00

  • Candida albicans secreted aspartyl proteinases: isoenzyme pattern is determined by cell type, and levels are determined by environmental factors.

    abstract::For the pathogenic yeast Candida albicans, secreted aspartyl proteinase (Sap) activity has been correlated with virulence. A family consisting of at least eight SAP genes can be drawn upon to produce Sap enzymatic activity. In this study, the levels of Sap1, Sap2, and Sap3 isoenzymes were monitored under a variety of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.18.5215-5221.1995

    authors: White TC,Agabian N

    更新日期:1995-09-01 00:00:00

  • Properties of pGC1, a lac plasmid orginating in Yersinia enterocolitica 842.

    abstract::pGC1, a self-transmissible lac plasmid that originated in Yersinia enterocolitica 842, is described. This plasmid is freely transmissible between strains of Y. enterocolitica and from such strains to strains of Escherichia coli. The plasmid can also be transferref among G. coli strains and back to Y. enterocolitica, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.3.1058-1062.1976

    authors: Cornelis G,Bennett PM,Grinsted J

    更新日期:1976-09-01 00:00:00

  • Glucose-6-phosphate dehydrogenase from the chemolithotroph Thiobacillus ferrooxidans.

    abstract::Glucose-6-phosphate dehydrogenase was partially purified from both glucose-grown and iron-glucose-grown Thiobacillus ferrooxidans. The enzyme possesses a dual nucleotide specificity for either nicotinamide adenine dinucleotide phosphate (NADP) or nicotinamide adenine dinucleotide (NAD) and has a molecular weight of 11...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.1.343-352.1971

    authors: Tabita R,Lundgren DG

    更新日期:1971-10-01 00:00:00

  • The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus.

    abstract::Two temperature-sensitive mutations, ams-1 and rne-3071, in the ams (altered mRNA stability) gene have been used extensively to investigate the processing and decay of RNA in Escherichia coli. We have sequenced these temperature-sensitive alleles and found that the mutations are separated by only 6 nucleotides and cau...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.13.4245-4249.1993

    authors: McDowall KJ,Hernandez RG,Lin-Chao S,Cohen SN

    更新日期:1993-07-01 00:00:00

  • Contribution of the periplasmic chaperone Skp to efficient presentation of the autotransporter IcsA on the surface of Shigella flexneri.

    abstract::IcsA is an outer membrane protein in the autotransporter family that is required for Shigella flexneri pathogenesis. Following its secretion through the Sec translocon, IcsA is incorporated into the outer membrane in a process that depends on YaeT, a component of an outer membrane beta-barrel insertion machinery. We i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00989-08

    authors: Wagner JK,Heindl JE,Gray AN,Jain S,Goldberg MB

    更新日期:2009-02-01 00:00:00

  • Vibrio vulnificus secretes a broad-specificity metalloprotease capable of interfering with blood homeostasis through prothrombin activation and fibrinolysis.

    abstract::Vibrio vulnificus is a causative agent of serious food-borne diseases in humans related to the consumption of raw seafood. It secretes a metalloprotease that is associated with skin lesions and serious hemorrhagic complications. In this study, we purified and characterized an extracellular metalloprotease (designated ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.20.6909-6916.2005

    authors: Chang AK,Kim HY,Park JE,Acharya P,Park IS,Yoon SM,You HJ,Hahm KS,Park JK,Lee JS

    更新日期:2005-10-01 00:00:00

  • Apical vesicles in growing bud cells of heterobasidiomycetous yeasts.

    abstract::Clusters of cytoplasmic vesicles resembling those in growing hyphal apices of mycelial fungi are found near the tips of buds in three heterobasidiomycetous yeasts, Rhodotorula glutinis, Candida scottii, and Sporobolomyces salmonicolor. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.2.922-926.1972

    authors: McCully EK,Bracker CE

    更新日期:1972-02-01 00:00:00

  • Genomic basis of a polyagglutinating isolate of Neisseria meningitidis.

    abstract::Containment strategies for outbreaks of invasive Neisseria meningitidis disease are informed by serogroup assays that characterize the polysaccharide capsule. We sought to uncover the genomic basis of conflicting serogroup assay results for an isolate (M16917) from a patient with acute meningococcal disease. To this e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06604-11

    authors: Rishishwar L,Katz LS,Sharma NV,Rowe L,Frace M,Dolan Thomas J,Harcourt BH,Mayer LW,Jordan IK

    更新日期:2012-10-01 00:00:00

  • GadY, a small-RNA regulator of acid response genes in Escherichia coli.

    abstract::A previous bioinformatics-based search for small RNAs in Escherichia coli identified a novel RNA named IS183. The gene encoding this small RNA is located between and on the opposite strand of genes encoding two transcriptional regulators of the acid response, gadX (yhiX) and gadW (yhiW). Given that IS183 is encoded in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.20.6698-6705.2004

    authors: Opdyke JA,Kang JG,Storz G

    更新日期:2004-10-01 00:00:00

  • Inactivation of the RluD pseudouridine synthase has minimal effects on growth and ribosome function in wild-type Escherichia coli and Salmonella enterica.

    abstract::The Escherichia coli rluD gene encodes a pseudouridine synthase responsible for the pseudouridine (Ψ) modifications at positions 1911, 1915, and 1917 in helix 69 of 23S rRNA. It has been reported that deletion of rluD in K-12 strains of E. coli is associated with extremely slow growth, increased readthrough of stop co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00970-10

    authors: O'Connor M,Gregory ST

    更新日期:2011-01-01 00:00:00

  • The dimerization function of MinC resides in a structurally autonomous C-terminal domain.

    abstract::Limited proteolysis of the Escherichia coli cell division inhibitor MinC reveals that its dimerization function resides in a structurally autonomous C-terminal domain. We show that cytoplasmic MinC is poised near the monomer-dimer equilibrium and propose that it only becomes entirely dimeric once recruited to the memb...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.22.6684-6687.2001

    authors: Szeto TH,Rowland SL,King GF

    更新日期:2001-11-01 00:00:00

  • Tyrosine phosphorylation of a membrane protein from Pseudomonas solanacearum.

    abstract::We have investigated a tyrosine kinase activity from Pseudomonas solanacearum, an economically important plant pathogen. In vitro incubation of membrane fractions with [gamma-32P]ATP and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed an 85-kDa phosphoprotein. Phosphorylation of this prot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.13.4356-4360.1992

    authors: Atkinson M,Allen C,Sequeira L

    更新日期:1992-07-01 00:00:00