Characterization of the regulatory region of a cell interaction-dependent gene in Myxococcus xanthus.


:omega 4403 is the site of a Tn5 lac insertion in the Myxococcus xanthus genome that fuses lacZ expression to a developmentally regulated promoter. Cell-cell interactions that occur during development, including C-signaling, are required for expression of Tn5 lac omega 4403. We have cloned DNA upstream of the omega 4403 insertion site, localized the promoter, and identified a potential open reading frame. From the deduced amino acid sequence, the gene disrupted by Tn5 lac omega 4403 appears to encode a serine protease that is dispensable for development. The gene begins to be expressed between 6 and 12 h after starvation initiates development, as determined by measuring mRNA or beta-galactosidase accumulation in cells containing Tn5 lac omega 4403. The putative transcriptional start site was mapped, and sequences centered near -10 and -35 bp relative to this site show some similarity to the corresponding regions of promoters transcribed by Escherichia coli sigma70 RNA polymerase. However, deletions showed that an essential promoter element lies between -80 and -72 bp, suggesting the possible involvement of an upstream activator protein. DNA downstream of -80 is sufficient for C-signal-dependent activation of this promoter. The promoter is not fully expressed when fusions are integrated at the Mx8 phage attachment site in the chromosome. Titration of a limiting factor by two copies of the regulatory region (one at the attachment site and one at the native site) can, in part, explain the reduced expression. We speculate that the remaining difference may be due to an effect of chromosomal position. These results provide a basis for studies aimed at identifying regulators of C-signal-dependent gene expression.


J Bacteriol


Journal of bacteriology


Fisseha M,Gloudemans M,Gill RE,Kroos L




Has Abstract


1996-05-01 00:00:00












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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: White TG,Puls JL

    更新日期:1969-05-01 00:00:00

  • Biosynthesis of streptococcal cell walls: N-acetyl-D-muramic acid.

    abstract::Glucose-1-(14)C and acetylglucosamine-1-(14)C were added singly and together with equal amounts of the unlabeled reciprocal to Brain Heart Infusion and used for the culture of Streptococcus pyogenes. The labeling pattern of the rhamnose, glucosamine, and muramic acid in the cell wall supported an intermediary role for...

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    pub_type: 杂志文章


    authors: Barkulis SS,Boltralik JJ,Hankin H,Heymann H

    更新日期:1967-10-01 00:00:00

  • Passive cutaneous anaphylaxis with antigens from Coxiella burneti.

    abstract::Passive cutaneous anaphylaxis (PCA) was produced in guinea pigs sensitized with guinea pig Coxiella burneti phase I-II antiserum and challenged with dimethylsulfoxide- or trichloroacetic acid-soluble extracts from phase I cells. The PCA reaction could not be induced by whole or mechanically disrupted phase I or phase ...

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    pub_type: 杂志文章


    authors: Peacock M,Munoz J,Tallent GL,Ormsbee RA

    更新日期:1968-05-01 00:00:00

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    abstract::A chemotaxis system for Pseudomonas aeruginosa was defined by using the method of Adler. Cells were attracted to compounds in the order ammonium chloride greater than amino acids greater than organic acids. Two sugars were assayed and elicited no response. Comparisons with other model systems are discussed. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Moench TT,Konetzka WA

    更新日期:1978-01-01 00:00:00

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    abstract::Regulation of the katB catalase gene in the anaerobic bacterium Bacteroides fragilis was studied. Northern blot hybridization analyses revealed that katB was transcribed as an approximately 1.6-kb monocistronic mRNA. The levels of katB mRNA increased > 15-fold when anaerobic, mid-logarithmic-phase cultures were expose...

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    authors: Rocha ER,Smith CJ

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    pub_type: 杂志文章


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    更新日期:1994-12-01 00:00:00

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    pub_type: 杂志文章


    authors: LeDeaux JR,Yu N,Grossman AD

    更新日期:1995-02-01 00:00:00

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    pub_type: 杂志文章


    authors: Washburn BK,Kushner SR

    更新日期:1991-04-01 00:00:00

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    pub_type: 杂志文章


    authors: Torres-Cabassa A,Gottesman S,Frederick RD,Dolph PJ,Coplin DL

    更新日期:1987-10-01 00:00:00

  • Tryptophan synthetase levels in Escherichia coli, Shigella dysenteriae, and transduction hybrids.

    abstract::Eisenstein, Richard B. (Western Reserve University, Cleveland, Ohio) and Charles Yanofsky. Tryptophan synthetase levels in Escherichia coli, Shigella dysenteriae, and transduction hybrids. J. Bacteriol. 83:193-204. 1962-Shigella dysenteriae and Escherichia coli, strains K-12 and B, were found to produce low levels of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1962-01-01 00:00:00


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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: GUNDERSEN WB

    更新日期:1963-09-01 00:00:00

  • Selective mRNA degradation by polynucleotide phosphorylase in cold shock adaptation in Escherichia coli.

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    journal_title:Journal of bacteriology

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    abstract::Specific growth rates as low as 0.005 hr(-1) (generation times of 20 to 200 hr) of aquatic bacteria in natural waters have been calculated from significant differences between dilution rates and washout rates in a chemostat. The measured growth rates were affected by the treatment of the water samples (type of sterili...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jannasch HW

    更新日期:1969-07-01 00:00:00

  • Purification and characterization of PBP4a, a new low-molecular-weight penicillin-binding protein from Bacillus subtilis.

    abstract::Penicillin-binding protein 4a (PBP4a) from Bacillus subtilis was overproduced and purified to homogeneity. It clearly exhibits DD-carboxypeptidase and thiolesterase activities in vitro. Although highly isologous to the Actinomadura sp. strain R39 DD-peptidase (B. Granier, C. Duez, S. Lepage, S. Englebert, J. Dusart, O...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Duez C,Vanhove M,Gallet X,Bouillenne F,Docquier J,Brans A,Frère J

    更新日期:2001-03-01 00:00:00

  • Fructose 1,6-bisphosphate aldolase activity is essential for synthesis of alginate from glucose by Pseudomonas aeruginosa.

    abstract::We have isolated a mutant of Pseudomonas aeruginosa deficient in fructose 1,6-bisphosphate aldolase activity. This mutant, similar to the mutants deficient in any of the Entner-Doudoroff pathway enzymes, does not allow appreciable alginate formation from glucose and gluconate, but allows alginate synthesis from mannit...

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    pub_type: 杂志文章


    authors: Banerjee PC,Vanags RI,Chakrabarty AM,Maitra PK

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  • Localization and solubilization of colicin receptors.

    abstract::Envelope fractions isolated from Escherichia coli K-12 C600 and from colicin-resistant and colicin-tolerant (Tol II) mutants derived from this strain were separated on sucrose gradients into cell wall-enriched and cytoplasmic membrane-enriched fractions. These fractions were tested for their ability to neutralize coli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sabet SF,Schnaitman CA

    更新日期:1971-10-01 00:00:00

  • LytR-CpsA-Psr enzymes as determinants of Bacillus anthracis secondary cell wall polysaccharide assembly.

    abstract::Bacillus anthracis, the causative agent of anthrax, replicates as chains of vegetative cells by regulating the separation of septal peptidoglycan. Surface (S)-layer proteins and associated proteins (BSLs) function as chain length determinants and bind to the secondary cell wall polysaccharide (SCWP). In this study, we...

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    更新日期:2015-01-01 00:00:00

  • Isolation of covalently closed circular deoxyribonucleic acid from Streptomyces coelicolor A3(2).

    abstract::Covalently closed circular deoxyribonucleic acid (DNA) was isolated from two strains of Streptomyces coelicolor A3(2), representing two of the known fertility types. In each of the two strains circular DNA of about 20 times 10-6 daltons could be detected, amounting to about 1.5% of the total cellular DNA. The possible...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Schrempf H,Bujard H,Hopwood DA,Goebel W

    更新日期:1975-02-01 00:00:00

  • Regulation of Aspergillus nidulans penicillin biosynthesis and penicillin biosynthesis genes acvA and ipnA by glucose.

    abstract::Expression of the Aspergillus nidulans penicillin biosynthesis genes acvA and ipnA, encoding delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase and isopenicillin N synthetase, respectively, was analyzed. The intergenic region carrying the divergently oriented promoters was fused in frame in both orientations ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Brakhage AA,Browne P,Turner G

    更新日期:1992-06-01 00:00:00

  • Complementary functioning of the component proteins of nitrogenase from several bacteria.

    abstract::The nitrogenase proteins from eight organisms have been highly purified, and a survey of their cross-reactions shows that the nitrogenase proteins from a wide variety of organisms can interact with one another. An active cross-reaction is the complementary functioning of the MoFe protein and the Fe protein from differ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Emerich DW,Burris RH

    更新日期:1978-06-01 00:00:00

  • Genome sequence of Lactobacillus fructivorans KCTC 3543.

    abstract::Lactobacillus fructivorans is important in the generation of particular flavors and in other ripening processes associated with fermented food. Here, we present the draft genome sequence of the type strain Lactobacillus fructivorans KCTC 3543 (1,373,326 bp, with a G+C content of 38.9%), which consists of 5 scaffolds. ...

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    pub_type: 杂志文章


    authors: Nam SH,Choi SH,Kang A,Lee KS,Kim DW,Kim RN,Kim DS,Park HS

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    abstract::The regulon of the sigma factor RpoS was defined in Geobacter sulfurreducens by using a combination of DNA microarray expression profiles and proteomics. An rpoS mutant was examined under steady-state conditions with acetate as an electron donor and fumarate as an electron acceptor and with additional transcriptional ...

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    authors: Núñez C,Esteve-Núñez A,Giometti C,Tollaksen S,Khare T,Lin W,Lovley DR,Methé BA

    更新日期:2006-04-01 00:00:00

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    abstract::Twenty-six FliF monomers assemble into the MS ring, a central motor component of the bacterial flagellum that anchors the structure in the inner membrane. Approximately 100 amino acids at the C terminus of FliF are exposed to the cytoplasm and, through the interaction with the FliG switch protein, a component of the f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Grünenfelder B,Gehrig S,Jenal U

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


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    更新日期:1994-10-01 00:00:00

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    abstract::The gene that encodes the acyl carrier protein (ACP) of the actinorhodin polyketide synthase (PKS) of Streptomyces coelicolor A3(2) was replaced with homologs from the granaticin, oxytetracycline, tetracenomycin, and putative frenolicin polyketide synthase gene clusters. All of the replacements led to expression of fu...

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    pub_type: 杂志文章


    authors: Khosla C,McDaniel R,Ebert-Khosla S,Torres R,Sherman DH,Bibb MJ,Hopwood DA

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    abstract::CytA, a 27-kDa cytolytic crystal protein of Bacillus thuringiensis subsp. israelensis, is produced only at very low levels by recombinant Escherichia coli cells unless a 20-kDa B. thuringiensis subsp. israelensis protein is also present (K. M. McLean and H. R. Whiteley, J. Bacteriol. 169:1017-1023, 1987; L. F. Adams, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Visick JE,Whiteley HR

    更新日期:1991-03-01 00:00:00

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McCullough J,Herskowitz I

    更新日期:1979-04-01 00:00:00

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ueda K,Hsheh CW,Tosaki T,Shinkawa H,Beppu T,Horinouchi S

    更新日期:1998-10-01 00:00:00

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Brush GS,Bhatnagar SK,Bessman MJ

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Loprasert S,Atichartpongkun S,Whangsuk W,Mongkolsuk S

    更新日期:1997-06-01 00:00:00