Characterization of the regulatory region of a cell interaction-dependent gene in Myxococcus xanthus.

Abstract:

:omega 4403 is the site of a Tn5 lac insertion in the Myxococcus xanthus genome that fuses lacZ expression to a developmentally regulated promoter. Cell-cell interactions that occur during development, including C-signaling, are required for expression of Tn5 lac omega 4403. We have cloned DNA upstream of the omega 4403 insertion site, localized the promoter, and identified a potential open reading frame. From the deduced amino acid sequence, the gene disrupted by Tn5 lac omega 4403 appears to encode a serine protease that is dispensable for development. The gene begins to be expressed between 6 and 12 h after starvation initiates development, as determined by measuring mRNA or beta-galactosidase accumulation in cells containing Tn5 lac omega 4403. The putative transcriptional start site was mapped, and sequences centered near -10 and -35 bp relative to this site show some similarity to the corresponding regions of promoters transcribed by Escherichia coli sigma70 RNA polymerase. However, deletions showed that an essential promoter element lies between -80 and -72 bp, suggesting the possible involvement of an upstream activator protein. DNA downstream of -80 is sufficient for C-signal-dependent activation of this promoter. The promoter is not fully expressed when fusions are integrated at the Mx8 phage attachment site in the chromosome. Titration of a limiting factor by two copies of the regulatory region (one at the attachment site and one at the native site) can, in part, explain the reduced expression. We speculate that the remaining difference may be due to an effect of chromosomal position. These results provide a basis for studies aimed at identifying regulators of C-signal-dependent gene expression.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Fisseha M,Gloudemans M,Gill RE,Kroos L

doi

10.1128/jb.178.9.2539-2550.1996

subject

Has Abstract

pub_date

1996-05-01 00:00:00

pages

2539-50

issue

9

eissn

0021-9193

issn

1098-5530

journal_volume

178

pub_type

杂志文章
  • Induction of experimental chronic arthritis in rabbits by cell-free fragments of Erysipelothrix.

    abstract::A cell-free crude extract of Erysipelothrix rhusiopathiae injected by high pressure jet into the knee-joint of rabbits stimulated an acute, mild inflammatory reaction. Additional injections at 3-day intervals induced a chronic condition characterized by hyperplasia of the synovial cells and hypertrophy of the villi, d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.2.403-406.1969

    authors: White TG,Puls JL

    更新日期:1969-05-01 00:00:00

  • Biosynthesis of streptococcal cell walls: N-acetyl-D-muramic acid.

    abstract::Glucose-1-(14)C and acetylglucosamine-1-(14)C were added singly and together with equal amounts of the unlabeled reciprocal to Brain Heart Infusion and used for the culture of Streptococcus pyogenes. The labeling pattern of the rhamnose, glucosamine, and muramic acid in the cell wall supported an intermediary role for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.4.963-965.1967

    authors: Barkulis SS,Boltralik JJ,Hankin H,Heymann H

    更新日期:1967-10-01 00:00:00

  • Passive cutaneous anaphylaxis with antigens from Coxiella burneti.

    abstract::Passive cutaneous anaphylaxis (PCA) was produced in guinea pigs sensitized with guinea pig Coxiella burneti phase I-II antiserum and challenged with dimethylsulfoxide- or trichloroacetic acid-soluble extracts from phase I cells. The PCA reaction could not be induced by whole or mechanically disrupted phase I or phase ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.5.1580-1586.1968

    authors: Peacock M,Munoz J,Tallent GL,Ormsbee RA

    更新日期:1968-05-01 00:00:00

  • Chemotaxis in Pseudomonas aeruginosa.

    abstract::A chemotaxis system for Pseudomonas aeruginosa was defined by using the method of Adler. Cells were attracted to compounds in the order ammonium chloride greater than amino acids greater than organic acids. Two sugars were assayed and elicited no response. Comparisons with other model systems are discussed. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.1.427-429.1978

    authors: Moench TT,Konetzka WA

    更新日期:1978-01-01 00:00:00

  • Regulation of Bacteriodes fragilis katB mRNA by oxidative stress and carbon limitation.

    abstract::Regulation of the katB catalase gene in the anaerobic bacterium Bacteroides fragilis was studied. Northern blot hybridization analyses revealed that katB was transcribed as an approximately 1.6-kb monocistronic mRNA. The levels of katB mRNA increased > 15-fold when anaerobic, mid-logarithmic-phase cultures were expose...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.22.7033-7039.1997

    authors: Rocha ER,Smith CJ

    更新日期:1997-11-01 00:00:00

  • The URE2 protein regulates nitrogen catabolic gene expression through the GATAA-containing UASNTR element in Saccharomyces cerevisiae.

    abstract::Many of the gene products that participate in nitrogen metabolism are sensitive to nitrogen catabolite repression (NCR), i.e., their expression is decreased to low levels when readily used nitrogen sources such as asparagine are provided. Previous work has shown this NCR sensitivity requires the cis-acting UASNTR elem...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.24.7476-7483.1994

    authors: Coffman JA,el Berry HM,Cooper TG

    更新日期:1994-12-01 00:00:00

  • Different roles for KinA, KinB, and KinC in the initiation of sporulation in Bacillus subtilis.

    abstract::Activation (phosphorylation) of the transcription factor encoded by spo0A is essential for the initiation of sporulation in Bacillus subtilis. At least three histidine protein kinases are involved in the phosphorylation of Spo0A. Under some growth conditions, KinA was the primary kinase, but under other conditions, Ki...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.3.861-863.1995

    authors: LeDeaux JR,Yu N,Grossman AD

    更新日期:1995-02-01 00:00:00

  • Construction and analysis of deletions in the structural gene (uvrD) for DNA helicase II of Escherichia coli.

    abstract::DNA helicase II, the product of the uvrD gene, has been implicated in DNA repair, replication, and recombination. Because the phenotypes of individual uvrD alleles vary significantly, we constructed deletion-insertion mutations in the uvrD gene to determine the phenotype of cells lacking DNA helicase II. Deletion muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.8.2569-2575.1991

    authors: Washburn BK,Kushner SR

    更新日期:1991-04-01 00:00:00

  • Control of extracellular polysaccharide synthesis in Erwinia stewartii and Escherichia coli K-12: a common regulatory function.

    abstract::A primary determinant of pathogenicity in Erwinia stewartii is the production of extracellular polysaccharide (EPS). A single mutation can abolish both EPS synthesis and pathogenicity; both properties are restored by a single cosmid clone. Subcloning and insertion analysis have defined a single positive regulatory fun...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.10.4525-4531.1987

    authors: Torres-Cabassa A,Gottesman S,Frederick RD,Dolph PJ,Coplin DL

    更新日期:1987-10-01 00:00:00

  • Tryptophan synthetase levels in Escherichia coli, Shigella dysenteriae, and transduction hybrids.

    abstract::Eisenstein, Richard B. (Western Reserve University, Cleveland, Ohio) and Charles Yanofsky. Tryptophan synthetase levels in Escherichia coli, Shigella dysenteriae, and transduction hybrids. J. Bacteriol. 83:193-204. 1962-Shigella dysenteriae and Escherichia coli, strains K-12 and B, were found to produce low levels of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.1.193-204.1962

    authors: EISENSTEIN RB,YANOFSKY C

    更新日期:1962-01-01 00:00:00

  • NEW TYPE OF STREPTOMYCIN RESISTANCE RESULTING FROM ACTION OF THE EPISOMELIKE MUTATOR FACTOR IN ESCHERICHIA COLI.

    abstract::Gundersen, Wenche B. (Oslo University, Oslo, Norway). New type of streptomycin resistance resulting from action of the episomelike mutator factor in Escherichia coli. J. Bacteriol. 86:510-516. 1963.-Analyses have been performed to elucidate the genetic nature of the streptomycin resistance that results from the action...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.3.510-516.1963

    authors: GUNDERSEN WB

    更新日期:1963-09-01 00:00:00

  • Selective mRNA degradation by polynucleotide phosphorylase in cold shock adaptation in Escherichia coli.

    abstract::Upon cold shock, Escherichia coli cell growth transiently stops. During this acclimation phase, specific cold shock proteins (CSPs) are highly induced. At the end of the acclimation phase, their synthesis is reduced to new basal levels, while the non-cold shock protein synthesis is resumed, resulting in cell growth re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.9.2808-2816.2001

    authors: Yamanaka K,Inouye M

    更新日期:2001-05-01 00:00:00

  • Estimations of bacterial growth rates in natural waters.

    abstract::Specific growth rates as low as 0.005 hr(-1) (generation times of 20 to 200 hr) of aquatic bacteria in natural waters have been calculated from significant differences between dilution rates and washout rates in a chemostat. The measured growth rates were affected by the treatment of the water samples (type of sterili...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.99.1.156-160.1969

    authors: Jannasch HW

    更新日期:1969-07-01 00:00:00

  • Purification and characterization of PBP4a, a new low-molecular-weight penicillin-binding protein from Bacillus subtilis.

    abstract::Penicillin-binding protein 4a (PBP4a) from Bacillus subtilis was overproduced and purified to homogeneity. It clearly exhibits DD-carboxypeptidase and thiolesterase activities in vitro. Although highly isologous to the Actinomadura sp. strain R39 DD-peptidase (B. Granier, C. Duez, S. Lepage, S. Englebert, J. Dusart, O...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.5.1595-1599.2001

    authors: Duez C,Vanhove M,Gallet X,Bouillenne F,Docquier J,Brans A,Frère J

    更新日期:2001-03-01 00:00:00

  • Fructose 1,6-bisphosphate aldolase activity is essential for synthesis of alginate from glucose by Pseudomonas aeruginosa.

    abstract::We have isolated a mutant of Pseudomonas aeruginosa deficient in fructose 1,6-bisphosphate aldolase activity. This mutant, similar to the mutants deficient in any of the Entner-Doudoroff pathway enzymes, does not allow appreciable alginate formation from glucose and gluconate, but allows alginate synthesis from mannit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.458-460.1985

    authors: Banerjee PC,Vanags RI,Chakrabarty AM,Maitra PK

    更新日期:1985-01-01 00:00:00

  • Localization and solubilization of colicin receptors.

    abstract::Envelope fractions isolated from Escherichia coli K-12 C600 and from colicin-resistant and colicin-tolerant (Tol II) mutants derived from this strain were separated on sucrose gradients into cell wall-enriched and cytoplasmic membrane-enriched fractions. These fractions were tested for their ability to neutralize coli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.1.422-430.1971

    authors: Sabet SF,Schnaitman CA

    更新日期:1971-10-01 00:00:00

  • LytR-CpsA-Psr enzymes as determinants of Bacillus anthracis secondary cell wall polysaccharide assembly.

    abstract::Bacillus anthracis, the causative agent of anthrax, replicates as chains of vegetative cells by regulating the separation of septal peptidoglycan. Surface (S)-layer proteins and associated proteins (BSLs) function as chain length determinants and bind to the secondary cell wall polysaccharide (SCWP). In this study, we...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02364-14

    authors: Liszewski Zilla M,Chan YG,Lunderberg JM,Schneewind O,Missiakas D

    更新日期:2015-01-01 00:00:00

  • Isolation of covalently closed circular deoxyribonucleic acid from Streptomyces coelicolor A3(2).

    abstract::Covalently closed circular deoxyribonucleic acid (DNA) was isolated from two strains of Streptomyces coelicolor A3(2), representing two of the known fertility types. In each of the two strains circular DNA of about 20 times 10-6 daltons could be detected, amounting to about 1.5% of the total cellular DNA. The possible...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.2.416-421.1975

    authors: Schrempf H,Bujard H,Hopwood DA,Goebel W

    更新日期:1975-02-01 00:00:00

  • Regulation of Aspergillus nidulans penicillin biosynthesis and penicillin biosynthesis genes acvA and ipnA by glucose.

    abstract::Expression of the Aspergillus nidulans penicillin biosynthesis genes acvA and ipnA, encoding delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase and isopenicillin N synthetase, respectively, was analyzed. The intergenic region carrying the divergently oriented promoters was fused in frame in both orientations ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.11.3789-3799.1992

    authors: Brakhage AA,Browne P,Turner G

    更新日期:1992-06-01 00:00:00

  • Complementary functioning of the component proteins of nitrogenase from several bacteria.

    abstract::The nitrogenase proteins from eight organisms have been highly purified, and a survey of their cross-reactions shows that the nitrogenase proteins from a wide variety of organisms can interact with one another. An active cross-reaction is the complementary functioning of the MoFe protein and the Fe protein from differ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.3.936-943.1978

    authors: Emerich DW,Burris RH

    更新日期:1978-06-01 00:00:00

  • Genome sequence of Lactobacillus fructivorans KCTC 3543.

    abstract::Lactobacillus fructivorans is important in the generation of particular flavors and in other ripening processes associated with fermented food. Here, we present the draft genome sequence of the type strain Lactobacillus fructivorans KCTC 3543 (1,373,326 bp, with a G+C content of 38.9%), which consists of 5 scaffolds. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00075-12

    authors: Nam SH,Choi SH,Kang A,Lee KS,Kim DW,Kim RN,Kim DS,Park HS

    更新日期:2012-04-01 00:00:00

  • DNA microarray and proteomic analyses of the RpoS regulon in Geobacter sulfurreducens.

    abstract::The regulon of the sigma factor RpoS was defined in Geobacter sulfurreducens by using a combination of DNA microarray expression profiles and proteomics. An rpoS mutant was examined under steady-state conditions with acetate as an electron donor and fumarate as an electron acceptor and with additional transcriptional ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.8.2792-2800.2006

    authors: Núñez C,Esteve-Núñez A,Giometti C,Tollaksen S,Khare T,Lin W,Lovley DR,Methé BA

    更新日期:2006-04-01 00:00:00

  • Role of the cytoplasmic C terminus of the FliF motor protein in flagellar assembly and rotation.

    abstract::Twenty-six FliF monomers assemble into the MS ring, a central motor component of the bacterial flagellum that anchors the structure in the inner membrane. Approximately 100 amino acids at the C terminus of FliF are exposed to the cytoplasm and, through the interaction with the FliG switch protein, a component of the f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.5.1624-1633.2003

    authors: Grünenfelder B,Gehrig S,Jenal U

    更新日期:2003-03-01 00:00:00

  • Characterization of lipoprotein EnvA in Chlamydia psittaci 6BC.

    abstract::The primary sequence of the small cysteine-rich protein (EnvA) of Chlamydia psittaci 6BC has been shown to possess a potential lipid modification/signal peptidase II-processing site, and the mature protein was labeled by a [3H]palmitic acid precursor. We further characterized the mature EnvA, showing that it lacks the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.19.6082-6087.1994

    authors: Everett KD,Desiderio DM,Hatch TP

    更新日期:1994-10-01 00:00:00

  • Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins.

    abstract::The gene that encodes the acyl carrier protein (ACP) of the actinorhodin polyketide synthase (PKS) of Streptomyces coelicolor A3(2) was replaced with homologs from the granaticin, oxytetracycline, tetracenomycin, and putative frenolicin polyketide synthase gene clusters. All of the replacements led to expression of fu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.8.2197-2204.1993

    authors: Khosla C,McDaniel R,Ebert-Khosla S,Torres R,Sherman DH,Bibb MJ,Hopwood DA

    更新日期:1993-04-01 00:00:00

  • Effect of a 20-kilodalton protein from Bacillus thuringiensis subsp. israelensis on production of the CytA protein by Escherichia coli.

    abstract::CytA, a 27-kDa cytolytic crystal protein of Bacillus thuringiensis subsp. israelensis, is produced only at very low levels by recombinant Escherichia coli cells unless a 20-kDa B. thuringiensis subsp. israelensis protein is also present (K. M. McLean and H. R. Whiteley, J. Bacteriol. 169:1017-1023, 1987; L. F. Adams, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.5.1748-1756.1991

    authors: Visick JE,Whiteley HR

    更新日期:1991-03-01 00:00:00

  • Mating pheromones of Saccharomyces kluyveri: pheromone interactions between Saccharomyces kluyveri and Saccharomyces cerevisiae.

    abstract::Saccharomyces kluyveri is a heterothallic yeast with two allelic mating types denoted as a-k and alpha-k by analogy with Saccharomyces cerevisiae and from the work described here. S. kluyveri produces mating pheromones analogous to those of S. cerevisiae, but which appear to have different specificity. S. kluyveri thu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.1.146-154.1979

    authors: McCullough J,Herskowitz I

    更新日期:1979-04-01 00:00:00

  • Characterization of an A-factor-responsive repressor for amfR essential for onset of aerial mycelium formation in Streptomyces griseus.

    abstract::A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) is essential for the initiation of aerial mycelium formation in Streptomyces griseus. amfR is one of the genes which, when cloned on a low-copy-number plasmid, suppresses the aerial mycelium-negative phenotype of an A-factor-deficient mutant of S. griseus....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.19.5085-5093.1998

    authors: Ueda K,Hsheh CW,Tosaki T,Shinkawa H,Beppu T,Horinouchi S

    更新日期:1998-10-01 00:00:00

  • Bacteriophage T4 deoxynucleotide kinase: gene cloning and enzyme purification.

    abstract::Gene 1 of bacteriophage T4 has been cloned into a lambda pL expression vector, resulting in the overproduction of deoxynucleotide kinase. A procedure that includes affinity chromatography on Cibacron Blue F3GA-agarose has been used to purify milligram quantities of enzymes from a 2-liter culture. The enzyme has been p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.6.2935-2939.1990

    authors: Brush GS,Bhatnagar SK,Bessman MJ

    更新日期:1990-06-01 00:00:00

  • Isolation and analysis of the Xanthomonas alkyl hydroperoxide reductase gene and the peroxide sensor regulator genes ahpC and ahpF-oxyR-orfX.

    abstract::From Xanthomonas campestris pv. phaseoli, we have isolated by two independent methods genes involved in peroxide detoxification (ahpC and ahpF), a gene involved in peroxide sensing and transcription regulation (oxyR), and a gene of unknown function (orfX). Amino acid sequence analysis of AhpC, AhpF, and OxyR showed hi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.12.3944-3949.1997

    authors: Loprasert S,Atichartpongkun S,Whangsuk W,Mongkolsuk S

    更新日期:1997-06-01 00:00:00