The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus.

Abstract:

:Two temperature-sensitive mutations, ams-1 and rne-3071, in the ams (altered mRNA stability) gene have been used extensively to investigate the processing and decay of RNA in Escherichia coli. We have sequenced these temperature-sensitive alleles and found that the mutations are separated by only 6 nucleotides and cause conservative amino acid substitutions next to a possible nucleotide-binding site within the N-terminal domain of the Ams protein. Computer analysis revealed that the region altered by the mutations has extensive sequence similarity to a predicted gene product from the mre (murein pathway cluster e) locus of E. coli, which has been implicated previously in determining bacterial cell shape.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

McDowall KJ,Hernandez RG,Lin-Chao S,Cohen SN

doi

10.1128/jb.175.13.4245-4249.1993

subject

Has Abstract

pub_date

1993-07-01 00:00:00

pages

4245-9

issue

13

eissn

0021-9193

issn

1098-5530

journal_volume

175

pub_type

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