rec-2-dependent phage recombination in Haemophilus influenzae.

Abstract:

:The genetic transformation mutant Rd(DB117)rec- has a pleiotropic phenotype that includes reduced levels of phage recombination. Physical mapping experiments showed that this strain has a 78.5-kbp insertion in the rec-2 gene. The rec-2 dependence of phage recombination was reexamined to determine whether the defective phenotype in Rd(DB117)rec- was due to the simple disruption of the rec-2 gene or whether trans-acting factors from the inserted DNA were responsible. Analysis of strains with transposon insertions in the rec-2 gene showed that they were also defective for phage recombination. Therefore, the phage recombination defect was due solely to the disruption of the rec-2 gene. Strain KB6 is proficient for phage recombination but has a defect in genetic transformation resembling that of Rd(DB117)rec-. The transformation defect of KB6 could be complemented by the wild-type rec-2 gene, showing that the rec-2 contributions to genetic transformation and phage recombination were uncoupled in this strain. The rec-2-dependent phenotype of KB6 suggests that the rec-2 gene participates in genetic transformation and phage recombination in different ways.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kupfer DM,McCarthy D

doi

10.1128/jb.174.15.4960-4966.1992

keywords:

subject

Has Abstract

pub_date

1992-08-01 00:00:00

pages

4960-6

issue

15

eissn

0021-9193

issn

1098-5530

journal_volume

174

pub_type

杂志文章
  • The requirement for pneumococcal MreC and MreD is relieved by inactivation of the gene encoding PBP1a.

    abstract::MreC and MreD, along with the actin homologue MreB, are required to maintain the shape of rod-shaped bacteria. The depletion of MreCD in rod-shaped bacteria leads to the formation of spherical cells and the accumulation of suppressor mutations. Ovococcus bacteria, such as Streptococcus pneumoniae, lack MreB homologues...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05245-11

    authors: Land AD,Winkler ME

    更新日期:2011-08-01 00:00:00

  • Mechanism of adhesion of Alysiella bovis to glass surfaces.

    abstract::Alysiella bovis adheres to surfaces by means of short, ruthenium red-staining, rod-like fimbriae. The fimbriae remain associated with the cell envelope of A. bovis, even when sonicated or exposed sequentially to toluene, Triton X-100, lysozyme, ribonuclease, and deoxyribonuclease. Adhesion of outer membrane-derived ce...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.2.569-576.1984

    authors: Irvin RT,To M,Costerton JW

    更新日期:1984-11-01 00:00:00

  • Natural occurrence of four reversible flagellar phases in cultures of Salmonella mikawashima.

    abstract::Edwards, P. R. (Communicable Disease Center, U.S. Public Health Service, Atlanta, Ga.), R. Sakazaki, and I. Kato. Natural occurrence of four reversible flagellar phases in cultures of Salmonella mikawashima. J. Bacteriol. 84:99-103. 1962-Study of cultures of Salmonella mikawashima isolated from reptiles revealed that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.1.99-103.1962

    authors: EDWARDS PR,SAKAZAKI R,KATO I

    更新日期:1962-07-01 00:00:00

  • Occurrence of alpha-tocopherolquinone and alpha-tocopherolquinol in microorganisms.

    abstract::Both alpha-tocopherolquinol and alpha-tocopherolquinone were found in 56 of 93 strains of microorganisms examined. Organisms that contained these compounds included the single example of a eucaryotic alga, a Euglena, and a cyanobacterium (blue-green alga), 22 of 32 genera of bacteria, and 9 genera of yeasts. In the ba...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.3.1397-1402.1982

    authors: Hughes PE,Tove SB

    更新日期:1982-09-01 00:00:00

  • Assay and purification of S-adenosyl-L-methionine:precorrin-2 methyltransferase from Pseudomonas denitrificans.

    abstract::S-Adenosyl-L-methionine:precorrin-2 methyltransferase (SP2MT), which catalyzes the C-20 methylation of precorrin-2 to precorrin-3, was purified to homogeneity from extracts of a recombinant strain of Pseudomonas denitrificans derived from a cobalamin-overproducing strain. Ammonium sulfate fractionation followed by chr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.11.6245-6251.1990

    authors: Thibaut D,Couder M,Crouzet J,Debussche L,Cameron B,Blanche F

    更新日期:1990-11-01 00:00:00

  • The tdh and serA operons of Escherichia coli: mutational analysis of the regulatory elements of leucine-responsive genes.

    abstract::The tdh promoter of Escherichia coli is induced seven- to eightfold when cells are grown in the presence of exogenous leucine. A scheme was devised to select mutants that exhibited high constitutive expression of the tdh promoter. The mutations in these strains were shown to lie within a previously identified gene (lr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.19.5944-5953.1991

    authors: Rex JH,Aronson BD,Somerville RL

    更新日期:1991-10-01 00:00:00

  • Deletion of the spf (spot 42 RNA) gene of Escherichia coli.

    abstract::To investigate the function of spot 42 RNA, a small RNA of Escherichia coli, we constructed a strain in which spf, the structural gene for this RNA, is deleted. We achieved this by using a delta att phage lambda carrying a DNA fragment spanning the spf region but with a precise deletion of spf. By integration of this ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.3.746-750.1986

    authors: Hatfull GF,Joyce CM

    更新日期:1986-06-01 00:00:00

  • YqfS from Bacillus subtilis is a spore protein and a new functional member of the type IV apurinic/apyrimidinic-endonuclease family.

    abstract::The enzymatic properties and the physiological function of the type IV apurinic/apyrimidinic (AP)-endonuclease homolog of Bacillus subtilis, encoded by yqfS, a gene specifically expressed in spores, were studied here. To this end, a recombinant YqfS protein containing an N-terminal His6 tag was synthesized in Escheric...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.18.5380-5390.2003

    authors: Salas-Pacheco JM,Urtiz-Estrada N,Martínez-Cadena G,Yasbin RE,Pedraza-Reyes M

    更新日期:2003-09-01 00:00:00

  • The penicillinase of Bacillus licheniformis is an outer membrane protein in Escherichia coli.

    abstract::The cloned gene coding for Bacillus licheniformis penicillinase (penP) was introduced into Escherichia coli in a heat-inducible lambda Qam vector. After induction, significant amounts of penicillinase were synthesized in the new host. The cellular location of the penicillinase was found to be almost exclusively the ou...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.2.657-663.1983

    authors: Sarvas MO,Palva IA

    更新日期:1983-08-01 00:00:00

  • Tryptophan operon regulation in interspecific hybrids of enteric bacteria.

    abstract::We examined tryptophan regulation in merodiploid hybrids in which a plasmid carrying the trp operon of Escherichia was introduced into Trp mutants of other enteric genera, or in which a plasmid carrying the trpR+ (repressor) gene of E. coli was transfered into fully constitutive trpR mutants of other genera. In these ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.2.679-689.1976

    authors: Manson MD,Yanofsky C

    更新日期:1976-05-01 00:00:00

  • nodO, a new nod gene of the Rhizobium leguminosarum biovar viciae sym plasmid pRL1JI, encodes a secreted protein.

    abstract::The region of the Rhizobium leguminosarum biovar viciae Sym plasmid pRL1JI, responsible for the production and secretion of a previously described 50-kilodalton protein (R. A. de Maagd, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg, J. Bacteriol. 170:4424-4427, 1988), was cloned and its nucleotide sequence was de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.12.6764-6770.1989

    authors: de Maagd RA,Wijfjes AH,Spaink HP,Ruiz-Sainz JE,Wijffelman CA,Okker RJ,Lugtenberg BJ

    更新日期:1989-12-01 00:00:00

  • Growth of flagellar filaments of Escherichia coli is independent of filament length.

    abstract::Bacterial flagellar filaments grow at their distal ends, from flagellin that travels through a central channel ∼2 nm in diameter. The flagellin is extruded from the cytoplasm by a pump powered by a proton motive force (PMF). We measured filament growth in cells near the mid-exponential-phase with flagellin bearing a s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06735-11

    authors: Turner L,Stern AS,Berg HC

    更新日期:2012-05-01 00:00:00

  • Inducible gamma-butyrobetaine-degrading enzymes in Pseudomonas species AK 1.

    abstract::Activities of gamma-butyrobetaine hydroxylase and carnitine dehydrogenase were low in cells of Pseudomonas sp. AK 1 grown in the absence of their respective substrates. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.3.1094-1095.1970

    authors: Lindstedt G,Lindstedt S,Midtvedt T,Tofft M

    更新日期:1970-03-01 00:00:00

  • OmpC and LamB proteins can serve as substitute receptors for host range mutants of coliphage TuIa.

    abstract::Coliphage TuIa, which uses the OmpF protein as a receptor, can adapt by mutation to using instead the OmpC or LamB protein, or both. Most of the phage mutants retained the ability to use the OmpF protein, when present, but one class of mutants lost this ability and could only use the OmpC protein. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.3.1182-1185.1980

    authors: Moreno F,Wandersman C

    更新日期:1980-12-01 00:00:00

  • Genetic characterization of Escherichia coli type 1 pilus adhesin mutants and identification of a novel binding phenotype.

    abstract::Five Escherichia coli type 1 pilus mutants that had point mutations in fimH, the gene encoding the type 1 pilus adhesin FimH, were characterized. FimH is a minor component of type 1 pili that is required for the pili to bind and agglutinate guinea pig erythrocytes in a mannose-inhibitable manner. Point mutations were ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.14.4012-4021.2000

    authors: Hamrick TS,Harris SL,Spears PA,Havell EA,Horton JR,Russell PW,Orndorff PE

    更新日期:2000-07-01 00:00:00

  • The tryptophanase gene cluster of Haemophilus influenzae type b: evidence for horizontal gene transfer.

    abstract::Among strains of Haemophilus influenzae, the ability to catabolize tryptophan (as detected by indole production) varies and is correlated with pathogenicity. Tryptophan catabolism is widespread (70 to 75%) among harmless respiratory isolates but is nearly universal (94 to 100%) among strains causing serious disease, i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.1.107-118.1998

    authors: Martin K,Morlin G,Smith A,Nordyke A,Eisenstark A,Golomb M

    更新日期:1998-01-01 00:00:00

  • Intermediate location in the assembly of the matrix protein or porin into the outer membrane of Escherichia coli.

    abstract::Evidence from pulse-chase experiments indicates that the outer membrane matrix protein or porin of Escherichia coli B/r passes through a Sarkosyl-soluble membrane pool on the way to its eventual Sarkosyl-insoluble state in the outer membrane. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.3.1538-1541.1980

    authors: Boyd A,Holland IB

    更新日期:1980-09-01 00:00:00

  • Nucleotide sequences of the gnd genes from nine natural isolates of Escherichia coli: evidence of intragenic recombination as a contributing factor in the evolution of the polymorphic gnd locus.

    abstract::Nine natural isolates of Escherichia coli were examined, and the sequence of the entire 1,404 bases of the gnd gene (6-phosphogluconate dehydrogenase, EC 1.1.1.44) was determined. These isolates, along with E. coli K-12, constitute 10 strains for analysis. (The sequence of the E. coli K-12 gnd gene is known.) A total ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.12.3894-3900.1991

    authors: Bisercić M,Feutrier JY,Reeves PR

    更新日期:1991-06-01 00:00:00

  • Synthesis of acid-soluble spore proteins by Bacillus subtilis.

    abstract::The major acid-soluble spore proteins (ASSPs) of Bacillus subtilis were detected by immunoprecipitation of radioactively labeled in vitro- and in vivo-synthesized proteins. ASSP synthesis in vivo began 2 h after the initiation of sporulation (t2) and reached its maximum rate at t7. This corresponded to the time of syn...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Leventhal JM,Chambliss GH

    更新日期:1982-12-01 00:00:00

  • The Xanthomonas oryzae pv. oryzae PhoPQ two-component system is required for AvrXA21 activity, hrpG expression, and virulence.

    abstract::The rice pathogen recognition receptor, XA21, confers resistance to Xanthomonas oryzae pv. oryzae strains producing the type one system-secreted molecule, AvrXA21. X. oryzae pv. oryzae requires a regulatory two-component system (TCS) called RaxRH to regulate expression of eight rax (required for AvrXA21 activity) gene...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01406-07

    authors: Lee SW,Jeong KS,Han SW,Lee SE,Phee BK,Hahn TR,Ronald P

    更新日期:2008-03-01 00:00:00

  • Genome sequences of Listeria monocytogenes strains J1816 and J1-220, associated with human outbreaks.

    abstract::Listeria monocytogenes has caused numerous human outbreaks. Here we report draft genomes of L. monocytogenes J1816 and J1-220, which belong to epidemic clones II and IV, respectively. Whole-genome sequence analysis of these strains provides a tool for studying the short-term evolution of these epidemic clones. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05048-11

    authors: Chen Y,Strain EA,Allard M,Brown EW

    更新日期:2011-07-01 00:00:00

  • Similarity in the size and number of ribosomal proteins from different prokaryotes.

    abstract::The ribosomal proteins from nine species of prokaryotes have been compared by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The stained gels were scanned spectrophotometrically, the weight and number average molecular weights were calculated, and the detailed distribution of the protein...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.111.2.474-480.1972

    authors: Sun TT,Bickle TA,Traut RR

    更新日期:1972-08-01 00:00:00

  • Diversity of Tn4001 transposition products: the flanking IS256 elements can form tandem dimers and IS circles.

    abstract::We show that both flanking IS256 elements carried by transposon Tn4001 are capable of generating head-to-tail tandem copies and free circular forms, implying that both are active. Our results suggest that the tandem structures arise from dimeric copies of the donor or vector plasmid present in the population by a mech...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.2.433-443.2002

    authors: Prudhomme M,Turlan C,Claverys JP,Chandler M

    更新日期:2002-01-01 00:00:00

  • Rhodobacter capsulatus OlsA is a bifunctional enzyme active in both ornithine lipid and phosphatidic acid biosynthesis.

    abstract::The Rhodobacter capsulatus genome contains three genes (olsA [plsC138], plsC316, and plsC3498) that are annotated as lysophosphatidic acid (1-acyl-sn-glycerol-3-phosphate) acyltransferase (AGPAT). Of these genes, olsA was previously shown to be an O-acyltransferase in the second step of ornithine lipid biosynthesis, w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01121-07

    authors: Aygun-Sunar S,Bilaloglu R,Goldfine H,Daldal F

    更新日期:2007-12-01 00:00:00

  • Altered muropeptide composition in Staphylococcus aureus strains with an inactivated femA locus.

    abstract::Tn551 inactivation of femA, a factor involved in methicillin resistance of Staphylococcus aureus, caused the production of peptidoglycan in which the fraction of monoglycyl- and serine-containing muropeptides was increased at the expense of pentaglycyl muropeptides. femA mutants have a specific block in the biosynthes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.9.2779-2782.1993

    authors: de Jonge BL,Sidow T,Chang YS,Labischinski H,Berger-Bachi B,Gage DA,Tomasz A

    更新日期:1993-05-01 00:00:00

  • Analysis of the regulation of gene expression during Bacillus subtilis sporulation by manipulation of the copy number of spo-lacZ fusions.

    abstract::The control of expression of the Bacillus subtilis spoIIA locus was analyzed by titrating gene expression against gene copy number. A plasmid integrated into the B. subtilis chromosome and carrying the spoIIA control region fused to Escherichia coli lacZ was forced to form tandem repeats by the selection of clones tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.3.1260-1266.1987

    authors: Piggot PJ,Curtis CA

    更新日期:1987-03-01 00:00:00

  • Identification of Coxiella burnetii type IV secretion substrates required for intracellular replication and Coxiella-containing vacuole formation.

    abstract::Coxiella burnetii, the etiological agent of acute and chronic Q fever in humans, is a naturally intracellular pathogen that directs the formation of an acidic Coxiella-containing vacuole (CCV) derived from the host lysosomal network. Central to its pathogenesis is a specialized type IVB secretion system (T4SS) that de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00071-13

    authors: Weber MM,Chen C,Rowin K,Mertens K,Galvan G,Zhi H,Dealing CM,Roman VA,Banga S,Tan Y,Luo ZQ,Samuel JE

    更新日期:2013-09-01 00:00:00

  • The eutD gene of Salmonella enterica encodes a protein with phosphotransacetylase enzyme activity.

    abstract::The EutD protein of Salmonella enterica is homologous to the catalytic domain of the phosphotransacetylase (Pta) enzyme. The Pta-like activity level of the EutD enzyme compared favorably to that of other Pta enzymes. High-pressure liquid chromatography and mass spectrometry verified that acetyl-coenzyme A was the prod...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.6.1890-1892.2004

    authors: Brinsmade SR,Escalante-Semerena JC

    更新日期:2004-03-01 00:00:00

  • Function and regulation of class I ribonucleotide reductase-encoding genes in mycobacteria.

    abstract::Ribonucleotide reductases (RNRs) are crucial to all living cells, since they provide deoxyribonucleotides (dNTPs) for DNA synthesis and repair. In Mycobacterium tuberculosis, a class Ib RNR comprising nrdE- and nrdF2-encoded subunits is essential for growth in vitro. Interestingly, the genome of this obligate human pa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01409-08

    authors: Mowa MB,Warner DF,Kaplan G,Kana BD,Mizrahi V

    更新日期:2009-02-01 00:00:00

  • Identification of genes encoding exported Mycobacterium tuberculosis proteins using a Tn552'phoA in vitro transposition system.

    abstract::Secreted and cell envelope-associated proteins are important to both Mycobacterium tuberculosis pathogenesis and the generation of protective immunity to M. tuberculosis. We used an in vitro Tn552'phoA transposition system to identify exported proteins of M. tuberculosis. The system is simple and efficient, and the tr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.10.2732-2740.2000

    authors: Braunstein M,Griffin TJ IV,Kriakov JI,Friedman ST,Grindley ND,Jacobs WR Jr

    更新日期:2000-05-01 00:00:00