Mechanism of adhesion of Alysiella bovis to glass surfaces.

Abstract:

:Alysiella bovis adheres to surfaces by means of short, ruthenium red-staining, rod-like fimbriae. The fimbriae remain associated with the cell envelope of A. bovis, even when sonicated or exposed sequentially to toluene, Triton X-100, lysozyme, ribonuclease, and deoxyribonuclease. Adhesion of outer membrane-derived cell wall ghosts of A. bovis to glass was inhibited by IO4-, sodium dodecyl sulfate, urea, pronase, and trypsin. Protease treatment digested the fimbriae from the distal end, and exposure to sodium dodecyl sulfate depolymerized the fimbriae. Exposure of ghosts to 1% sodium dodecyl sulfate preferentially solubilized a 16,500-dalton protein which was subsequently purified by gel filtration and demonstrated to be a glycoprotein (ca. 17% carbohydrate). Antibodies raised against the 16,500-dalton glycoprotein agglutinated whole cells and inhibited adhesion of ghosts to glass.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Irvin RT,To M,Costerton JW

doi

10.1128/JB.160.2.569-576.1984

subject

Has Abstract

pub_date

1984-11-01 00:00:00

pages

569-76

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

160

pub_type

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