Regulation of enzyme synthesis in the aromatic amino acid pathway of Bacillus subtilus.

Abstract:

:The control of the synthesis of certain key enzymes of aromatic amino acid biosynthesis was studied. Tyrosine represses the first enzyme of the 3-deoxy-d-arabino heptulosonic acid 7-phosphate pathway, DAHP synthetase, as well as shikimate kinase and chorismate mutase about fivefold in cultures grown under conditions limiting the synthesis of the aromatic amino acids. A mixture of tyrosine and phenylalanine represses twofold further. Tryptophan does not appear to be involved in the control of these enzymes. The specific activity of at least one early enzyme, dehydroquinase, remains essentially constant under a variety of nutritional supplementations. Two enzymes in the terminal branches are repressed by the amino acids they help to synthesize: prephenate dehydrogenase can be repressed fourfold by tyrosine, and anthranilate synthetase can be repressed over 200-fold by tryptophan. There is no evidence that phenylalanine represses prephenate dehydratase. Regulatory mutants have been isolated in which various enzymes of the pathway are no longer repressible. One class is derepressed for several of the prechorismate enzymes, as well as chorismate mutase and prephenate dehydrogenase. In another mutant, several enzymes of tryptophan biosynthesis are no longer repressible. Thus, the rate of synthesis of enzymes at every stage of the pathway is under control of various aromatic amino acids. Tyrosine and phenylalanine control the synthesis of enzymes involved in the synthesis of the three aromatic amino acids. Each terminal branch is under the control of its end product.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Nester EW,Jensen RA,Nasser DS

doi

10.1128/JB.97.1.83-90.1969

subject

Has Abstract

pub_date

1969-01-01 00:00:00

pages

83-90

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

97

pub_type

杂志文章
  • Genome sequence of proteorhodopsin-containing sea ice bacterium Glaciecola punicea ACAM 611T.

    abstract::Here, we report the draft genome sequence of Antarctic sea ice bacterium Glaciecola punicea ACAM 611(T), the type species of the genus Glaciecola. A blue-light-absorbing proteorhodopsin gene is present in the 3.08-Mb genome. This genome sequence can facilitate the study of the physiological metabolisms and ecological ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00463-12

    authors: Qin QL,Xie BB,Shu YL,Rong JC,Zhao DL,Zhang XY,Chen XL,Zhou BC,Zhang YZ

    更新日期:2012-06-01 00:00:00

  • Membrane chaperone SecDF plays a role in the secretion of Listeria monocytogenes major virulence factors.

    abstract::Listeria monocytogenes is a Gram-positive human intracellular pathogen that infects diverse mammalian cells. Upon invasion, L. monocytogenes secretes multiple virulence factors that target host cellular processes and promote infection. It has been presumed, but was not empirically established, that the Sec translocati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00697-13

    authors: Burg-Golani T,Pozniak Y,Rabinovich L,Sigal N,Nir Paz R,Herskovits AA

    更新日期:2013-12-01 00:00:00

  • Stringent regulation of the synthesis of a transfer ribonucleic acid biosynthetic enzyme: transfer ribonucleic acid(m5U)methyltransferase from Escherichia coli.

    abstract::This paper describes the regulation of a transfer ribonucleic acid (tRNA) biosynthetic enzyme, the tRNA(m5U)methyltransferase (EC 2.1.1.35). This enzyme catalyzes the formation of 5-methyluridine (m5U, ribothymidine) in all tRNA chains of Escherichia coli. Partial deprivation of charged tRNAVal can be imposed by shift...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.2.635-641.1977

    authors: Ny T,Björk GR

    更新日期:1977-05-01 00:00:00

  • Cloning and sequence analysis of the muramidase-2 gene from Enterococcus hirae.

    abstract::Extracellular muramidase-2 of Enterococcus hirae ATCC 9790 was purified to homogeneity by substrate binding, guanidine-HCl extraction, and reversed-phase chromatography. A monoclonal antibody, 2F8, which specifically recognizes muramidase-2, was used to screen a genomic library of E. hirae ATCC 9790 DNA in bacteriopha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.5.1619-1625.1992

    authors: Chu CP,Kariyama R,Daneo-Moore L,Shockman GD

    更新日期:1992-03-01 00:00:00

  • Effect of glucose starvation on the nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase of yeast.

    abstract::Yeast cells growing on mineral medium plus ammonia and glucose contained high levels of nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase activity, as measured in crude extracts. After suspension of cells in fresh medium lacking glucose, there was a loss of the glutamate dehydrogenase activ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.2.780-785.1978

    authors: Mazón MJ

    更新日期:1978-02-01 00:00:00

  • Identification of a second collagen-like glycoprotein produced by Bacillus anthracis and demonstration of associated spore-specific sugars.

    abstract::Certain carbohydrates (rhamnose, 3-O-methyl rhamnose, and galactosamine) have been demonstrated to be present in Bacillus anthracis spores but absent in vegetative cells. Others have demonstrated that these spore-specific sugars are constituents of the glycoprotein BclA. In the current work, spore extracts were separa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.13.4592-4597.2005

    authors: Waller LN,Stump MJ,Fox KF,Harley WM,Fox A,Stewart GC,Shahgholi M

    更新日期:2005-07-01 00:00:00

  • The Bacillus subtilis 168 alkaline phosphatase III gene: impact of a phoAIII mutation on total alkaline phosphatase synthesis.

    abstract::The first alkaline phosphatase (APase) structural gene mutant of Bacillus subtilis 168 was constructed by using a clone identified by hybridization to a synthetic degenerative oligonucleotide. The design of the probe was based on the first 29 amino acids of the sequenced mature APase III protein, which had been isolat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.7.3730-3737.1990

    authors: Bookstein C,Edwards CW,Kapp NV,Hulett FM

    更新日期:1990-07-01 00:00:00

  • Highly Contingent Phenotypes of Lon Protease Deficiency in Escherichia coli upon Antibiotic Challenge.

    abstract::Evolutionary trajectories and mutational landscapes of drug-resistant bacteria are influenced by cell-intrinsic and extrinsic factors. In this study, I demonstrated that loss of the Lon protease altered susceptibility of Escherichia coli to trimethoprim and that these effects were strongly contingent on the drug conce...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00561-19

    authors: Matange N

    更新日期:2020-01-15 00:00:00

  • Functional and structural analysis of HicA3-HicB3, a novel toxin-antitoxin system of Yersinia pestis.

    abstract::The mechanisms involved in the virulence of Yersinia pestis, the plague pathogen, are not fully understood. In previous research, we found that a Y. pestis mutant lacking the HicB3 (YPO3369) putative orphan antitoxin was attenuated for virulence in a murine model of bubonic plague. Toxin-antitoxin systems (TASs) are w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01932-14

    authors: Bibi-Triki S,Li de la Sierra-Gallay I,Lazar N,Leroy A,Van Tilbeurgh H,Sebbane F,Pradel E

    更新日期:2014-11-01 00:00:00

  • Genetic mapping of the chromosomal replication origin of Salmonella typhimurium.

    abstract::Two hundred strains of Escherichia coli harboring Filv+ plasmids which carry a segment of the Salmonella typhimurium chromosome were isolated independently. Among them, two strains were found to harbor F' plasmids that are able to replicate in Hfr cells of E. coli; i.e., they carry a site designated poh (permissive on...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.2.297-304.1979

    authors: Joh K,Hiraga S

    更新日期:1979-05-01 00:00:00

  • Distribution of an L-isoaspartyl protein methyltransferase in eubacteria.

    abstract::A protein carboxyl methyltransferase (EC 2.1.1.77) that recognizes age-damaged proteins for potential repair or degradation reactions has been found in all vertebrate tissues and cells examined to date. This enzyme catalyzes the transfer of methyl groups from S-adenosylmethionine to the carboxyl groups of D-aspartyl o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.2.355-361.1992

    authors: Li C,Clarke S

    更新日期:1992-01-01 00:00:00

  • Distinct Contributions of Enzymic Functional Groups to the 2',3'-Cyclic Phosphodiesterase, 3'-Phosphate Guanylylation, and 3'-ppG/5'-OH Ligation Steps of the Escherichia coli RtcB Nucleic Acid Splicing Pathway.

    abstract:UNLABELLED:Escherichia coli RtcB is a founding member of a family of manganese-dependent RNA repair enzymes that join RNA 2′,3′-cyclic phosphate (RNA>p) or RNA 3′-phosphate (RNAp) ends to 5′-OH RNA (HORNA) ends in a multistep pathway whereby RtcB (i) hydrolyzes RNA>p to RNAp, (ii) transfers GMP from GTP to RNAp to form...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00913-15

    authors: Maughan WP,Shuman S

    更新日期:2016-03-31 00:00:00

  • Polyphosphate and orthophosphate content of Nitrosomonas europaea as a function of growth.

    abstract::After inoculation of a stationary-phase culture of Nitrosomonas europaea into fresh growth solution, the cell-associated orthophosphate increased rapidly to 800 mumoles/g (wet weight), whereas the acid-insoluble long-chain polyphosphate content decreased rapidly to 22 mumoles/g. As growth proceeded, the orthophosphate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.103.1.199-206.1970

    authors: Terry KR,Hooper AB

    更新日期:1970-07-01 00:00:00

  • Analysis of the activity and regulon of the two-component regulatory system composed by Cjj81176_1484 and Cjj81176_1483 of Campylobacter jejuni.

    abstract:UNLABELLED:Campylobacter jejuni is a leading cause of bacterial diarrheal disease and a frequent commensal of the intestinal tract in poultry and other animals. For optimal growth and colonization of hosts, C. jejuni employs two-component regulatory systems (TCSs) to monitor environmental conditions and promote proper ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02564-14

    authors: Luethy PM,Huynh S,Parker CT,Hendrixson DR

    更新日期:2015-05-01 00:00:00

  • Effect of a 20-kilodalton protein from Bacillus thuringiensis subsp. israelensis on production of the CytA protein by Escherichia coli.

    abstract::CytA, a 27-kDa cytolytic crystal protein of Bacillus thuringiensis subsp. israelensis, is produced only at very low levels by recombinant Escherichia coli cells unless a 20-kDa B. thuringiensis subsp. israelensis protein is also present (K. M. McLean and H. R. Whiteley, J. Bacteriol. 169:1017-1023, 1987; L. F. Adams, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.5.1748-1756.1991

    authors: Visick JE,Whiteley HR

    更新日期:1991-03-01 00:00:00

  • Pneumolysin localizes to the cell wall of Streptococcus pneumoniae.

    abstract::Streptococcus pneumoniae is the causative agent of multiple diseases, including otitis media, pneumonia, bacteremia, and meningitis. Pneumolysin (Ply), a member of the cholesterol-dependent cytolytic pore-forming toxins, is produced by virtually all clinical isolates of S. pneumoniae, and strains in which the Ply gene...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01489-08

    authors: Price KE,Camilli A

    更新日期:2009-04-01 00:00:00

  • Enzymes involved in the assimilation of one-carbon units by Pseudomonas MS.

    abstract::Pseudomonas MS can grow on methylamine and a number of other compounds containing C1 units as a sole source of carbon and energy. Assimilation of carbon into cell material occurs via the "serine pathway" since enzymes of this pathway are induced after growth on methylamine, but not malate or acetate. A mutant has been...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.3.905-910.1975

    authors: Wagner C,Levitch ME

    更新日期:1975-06-01 00:00:00

  • Deoxyribonucleic acid synthesis in synchronously growing Mycoplasma gallisepticum.

    abstract::Early log-phase cells of Mycoplasma gallisepticum A5969 were synchronized by holding in Eagle minimal essential medium (MEM) for 2 h. When transferred out of MEM into tryptose medium, the cells exhibited synchronous growth. Deoxyribonucleic acid (DNA) synthesis proceeded continuously during this growth but stopped dur...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.1.117-120.1973

    authors: Quinlan DC,Maniloff J

    更新日期:1973-07-01 00:00:00

  • Mechanistically novel iron(III) transport system in Serratia marcescens.

    abstract::A novel iron(III) transport system of Serratia marcescens, named SFU, was cloned and characterized in Escherichia coli. Iron acquisition by this system differed from that by E. coli and related organisms. No siderophore production and no receptor protein related to the SFU system could be detected. In addition, iron u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.238-243.1989

    authors: Zimmermann L,Angerer A,Braun V

    更新日期:1989-01-01 00:00:00

  • Relative stability of membrane proteins in Escherichia coli.

    abstract::The relative stability of membrane proteins in Escherichia coli was investigated to determine whether these proteins are degraded at heterogeneous rates and, if so, whether the degradative rates are correlated with the sizes or charges of the proteins. Cells growing in a glucose-limited chemostat with a generation tim...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.2.476-483.1981

    authors: Schroer DW,St John AC

    更新日期:1981-05-01 00:00:00

  • Amino acid uptake and energy coupling dependent on photosynthesis in Anacystis nidulans.

    abstract::The photoautotrophic cyanobacterium Anacystis nidulans was used to investigate the membrane transport of branched-chain, neutral amino acids and its dependence on photosynthetic reactions. The uptake of alpha-amino [1-14C]isobutyric acid and L-[1-14C]leucine followed Michaelis, Menten kinetics and resulted in an energ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.1.229-236.1982

    authors: Lee-Kaden J,Simonis W

    更新日期:1982-07-01 00:00:00

  • Insertional inactivation of trpC in cloned Bacillus trp segments: evidence for a polar effect on trpF.

    abstract::Plasmid pUB110 was previously used as a vector to clone fragments of deoxyribonucleic acid that complement the trpC2 mutation in Bacillus subtilis from endonuclease EcoRI digested B. licheniformis, B. pumilus, and B. subtilis cellular deoxyribonucleic acid. Each of several such trp plasmids was subsequently shown to c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.3.1001-1006.1979

    authors: Keggins KM,Lovett PS,Marrero R,Hoch SO

    更新日期:1979-09-01 00:00:00

  • Regulation of the Escherichia coli hfq gene encoding the host factor for phage Q beta.

    abstract::The host factor (HF-I) for phage Q beta RNA replication is a small protein of 102 amino acid residues encoded by the hfq gene at 94.8 min on the Escherichia coli chromosome. The synthesis rate of HF-I at the exponential-growth phase is higher than at the stationary phase, and it increases concomitantly with the increa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.2.531-534.1994

    authors: Kajitani M,Kato A,Wada A,Inokuchi Y,Ishihama A

    更新日期:1994-01-01 00:00:00

  • Purification and characterization of a molybdenum-pterin-binding protein (Mop) in Clostridium pasteurianum W5.

    abstract::A large-scale fractionation scheme purified the major molybdenum(Mo)-binding protein (Mop) from crude extracts of Clostridium pasteurianum, with a 10 and 0.2% yield of Mo and protein, respectively. The apparent molecular weight of the purified molybdoprotein is 5,700, as determined by sodium dodecyl sulfate-polyacryla...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.2.688-693.1986

    authors: Hinton SM,Merritt B

    更新日期:1986-11-01 00:00:00

  • Participation of an extracellular deaminase in amino acid utilization by Neurospora crassa.

    abstract::A strain of Neurospora crassa defective in amino acid transport can utilize a variety of amino acids for growth when readily metabolizable nitrogen is limiting. Growth is accompanied by the production of an extracellular deaminase that converts the amino acid to its respective keto acid plus equimolar quantities of ut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.2.583-589.1984

    authors: DeBusk RM,Ogilvie S

    更新日期:1984-08-01 00:00:00

  • Identification of the lipopolysaccharide core region as the receptor site for a cytotoxin-converting phage, phi CTX, of Pseudomonas aeruginosa.

    abstract::A temperate phage, phi CTX, is a cytotoxin-converting phage of Pseudomonas aeruginosa. In this study, we characterized the lipopolysaccharide (LPS) structures of phi CTX-resistant mutants derived from phi CTX-sensitive strains. phi CTX infectivity was neutralized by LPS preparations derived from sensitive strains but ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.17.5262-5269.1994

    authors: Yokota S,Hayashi T,Matsumoto H

    更新日期:1994-09-01 00:00:00

  • Major membrane surface proteins of Mycoplasma hyopneumoniae selectively modified by covalently bound lipid.

    abstract::Surface protein antigens of Mycoplasma hyopneumoniae were identified by direct antibody-surface binding or by radioimmunoprecipitation of surface 125I-labeled proteins with a series of monoclonal antibodies (MAbs). Surface proteins p70, p65, p50, and p44 were shown to be integral membrane components by selective parti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.12.5546-5555.1987

    authors: Wise KS,Kim MF

    更新日期:1987-12-01 00:00:00

  • Contribution of σ70 and σN Factors to Expression of Class II pilE in Neisseria meningitidis.

    abstract::Neisseria meningitidis expresses multicomponent organelles called type four pili (Tfp), which are key virulence factors required for attachment to human cells during carriage and disease. Pilin (PilE) is the main component of Tfp, and N. meningitidis isolates either have a class I pilE locus and express pilins that un...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00170-19

    authors: Lobanovska M,Tang CM,Exley RM

    更新日期:2019-09-20 00:00:00

  • INDUCTION OF NONSPECIFIC RESISTANCE BY ENDOTOXIN IN UNRESPONSIVE MICE.

    abstract::Margherita, S. S. (Temple University School of Medicine, Philadelphia, Pa.), and H. Friedmann. Induction of nonspecific resistance by endotoxin in unresponsive mice. J. Bacteriol. 89:277-280. 1965.-Serratia marcescens endotoxin was tested for protective effect in animals of varying immunological competence. A quantity...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.2.277-280.1965

    authors: MARGHERITA SS,FRIEDMAN H

    更新日期:1965-02-01 00:00:00

  • Characterization of an operon encoding an NADP-reducing hydrogenase in Desulfovibrio fructosovorans.

    abstract::A genomic DNA fragment from Desulfovibrio fructosovorans, which strongly hybridized with the hydAB genes from Desulfovibrio vulgaris Hildenborough, was cloned and sequenced. This fragment was found to contain four genes, named hndA, hndB, hndC, and hndD. Analysis of the sequence homologies indicated that HndA shows 29...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.10.2628-2636.1995

    authors: Malki S,Saimmaime I,De Luca G,Rousset M,Dermoun Z,Belaich JP

    更新日期:1995-05-01 00:00:00