Abstract:
:The relative stability of membrane proteins in Escherichia coli was investigated to determine whether these proteins are degraded at heterogeneous rates and, if so, whether the degradative rates are correlated with the sizes or charges of the proteins. Cells growing in a glucose-limited chemostat with a generation time of 15 h were labeled with [(14)C]leucine. After allowing 24 h for turnover of (14)C-labeled proteins, the cells were labeled for 15 min with [(3)H]leucine. By this protocol, the rapidly degraded proteins have a high ratio of (3)H to (14)C, whereas the stable proteins have a lower ratio. The total cell envelope fraction was collected by differential centrifugation, and the proteins were separated by two-dimensional polyacrylamide gel electrophoresis. The relative ratio for each protein was determined by dividing its (3)H/(14)C ratio by the (3)H/(14)C ratio of the total membrane fraction. Although most of the 125 membrane proteins had relative ratios close to the average for the total membrane fraction, 19 varied significantly from this value. These differences were also observed when the order of addition of [(14)C]leucine and [(3)H]leucine was reversed. In control cultures labeled simultaneously with both isotopes, the relative ratios of these 19 proteins were similar to that of the total membrane fraction. Thirteen of these proteins had low relative ratios, which suggested that they were more stable than the average protein. An experiment in which the normal labeling procedure was followed by a 60-min chase period in the presence of excess unlabeled leucine suggested that the low relative ratios of 3 of these 13 proteins may be due to a slow post-translational modification step. Six membrane proteins had high relative ratios, which indicated that they were degraded rapidly. In contrast to the relationships found for soluble proteins in mammalian cells, there were no strong correlations between the degradative rates and either the isoelectric points or the molecular weights of membrane proteins in E. coli.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Schroer DW,St John ACdoi
10.1128/JB.146.2.476-483.1981subject
Has Abstractpub_date
1981-05-01 00:00:00pages
476-83issue
2eissn
0021-9193issn
1098-5530journal_volume
146pub_type
杂志文章abstract::During depletion of 4.5S RNA, cells of Escherichia coli displayed a heat shock response that was simultaneous with the first detectable effect on ribosome function and before major effects on cell growth. Either 4.5S RNA is involved directly in regulating the heat shock response, or this particular impairment of prote...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.1151-1154.1990
更新日期:1990-02-01 00:00:00
abstract::Mutants of Agrobacterium tumefaciens requiring methionine for growth on a solid basal medium were induced by the use of N-methyl-N'-nitro-N-nitrosoguanidine. In addition to the difference of mutant strains, the extent of methionine dependency differed in a liquid basal medium and in the presence of aspartate or fumara...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.6.2078-2082.1968
更新日期:1968-06-01 00:00:00
abstract::The deduced amino acid sequences of the flagellins of Pseudomonas syringae pv. tabaci and P. syringae pv. glycinea are identical; however, their abilities to induce a hypersensitive reaction are clearly different. The reason for the difference seems to depend on the posttranslational modification of the flagellins. To...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.22.6658-6665.2003
更新日期:2003-11-01 00:00:00
abstract::The coding region of the alpha-amylase inhibitor (HaimII) gene from the producing strain Streptomyces griseosporeus YM-25 was localized on an 800-base-pair DNA segment. The nucleotide sequence of a 1,191-base-pair region including the HaimII gene was determined by the dideoxy-chain termination method. The nucleotide s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4451-4457.1988
更新日期:1988-10-01 00:00:00
abstract::A chemotaxis signal transduction pathway (hereafter called Che1) has been previously identified in the alphaproteobacterium Azospirillum brasilense. Previous experiments have demonstrated that although mutants lacking CheB and/or CheR homologs from this pathway are defective in chemotaxis, a mutant in which the entire...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00734-08
更新日期:2008-10-01 00:00:00
abstract::A series of independent Escherichia coli K eductants has been isolated and tested to determine the extent of their deletions. The deletions cover the P2 prophage in location H, the his operon, a suppressor of the recBC phenotype (sbcB), the gene for gluconate-6-phosphate dehydrogenase (gnd), a locus involved in cell w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.2.695-704.1971
更新日期:1971-11-01 00:00:00
abstract::Strain IMCC9480 is a novel member of the family Oxalobacteraceae of the Betaproteobacteria, isolated from the Arctic Ocean by dilution-to-extinction culturing. Here we present the draft genome sequence of strain IMCC9480. The genome is predicted to contain genes for xanthorhodopsin, retinoid biosynthesis, carbon monox...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05088-11
更新日期:2011-07-01 00:00:00
abstract::The Escherichia coli gene coding for dihydropteroate synthase (DHPS) has been cloned and sequenced. The protein has 282 amino acids and a compositional molecular mass of 30,314 daltons. Increased expression of the enzyme was realized by using a T7 expression system. The enzyme was purified and crystallized. A temperat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.18.5961-5970.1992
更新日期:1992-09-01 00:00:00
abstract::A Synechocystis sp. strain PCC 6803 mutant lacking CtaI, a main subunit of cytochrome c oxidase, is not capable of growing at light intensities below 5 micromol photons m(-2) s(-1), presumably due to an overreduced plastoquinone pool in the thylakoid membrane. Upon selection for growth at light intensities below 5 mic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.4.1286-1294.2006
更新日期:2006-02-01 00:00:00
abstract::Survivors of nitrosoguanidine-treated cultures of a colicinogenic strain of Salmonella typhimurium were tested for spontaneous production of colicin E1. Of about 1,000 colonies tested, 13 produced no (or very narrow) colicin zones. Four of these isolates proved to be more sensitive to ultraviolet (UV) light, X rays, a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.345-350.1970
更新日期:1970-10-01 00:00:00
abstract::Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for worldwide outbreaks of bloody diarrhea, hemorrhagic colitis, and life-threatening hemolytic uremic syndrome. After colonizing the large intestine, EHEC forms attaching and effacing (AE) lesions on intestinal epithelial cells. These lesions cause dest...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01848-06
更新日期:2007-03-01 00:00:00
abstract::The genes involved in gluconate catabolism (gntP and gntK) in Corynebacterium glutamicum are scattered in the chromosome, and no regulatory genes are apparently associated with them, in contrast with the organization of the gnt operon in Escherichia coli and Bacillus subtilis. In C. glutamicum, gntP and gntK are essen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.2.409-423.2006
更新日期:2006-01-01 00:00:00
abstract::Those genes in Escherichia coli defined by mutations which result in an inability to grow at high temperatures are designated htr, indicating a high temperature requirement. A new htr mutant of E. coli was isolated and characterized and is designated htrD. The htrD gene has been mapped to 19.3 min on the E. coli chrom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.4.1240-1247.1992
更新日期:1992-02-01 00:00:00
abstract::A gene (yacK) encoding a putative multicopper oxidase (MCO) was cloned from Escherichia coli, and the expressed enzyme was demonstrated to exhibit phenoloxidase and ferroxidase activities. The purified protein contained six copper atoms per polypeptide chain and displayed optical and electron paramagnetic resonance (E...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.16.4866-4875.2001
更新日期:2001-08-01 00:00:00
abstract::H-NS is an abundant nucleoid-associated protein involved in the maintenance of chromosomal architecture in bacteria. H-NS also has a role in silencing the expression of a variety of environmentally regulated genes during growth under nonpermissive conditions. In this study we demonstrate a role for H-NS in the negativ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.15.4295-4303.2000
更新日期:2000-08-01 00:00:00
abstract::The orientation of lipoteichoic acid (LTA) molecules on the surface of bacterial cells undoubtedly is determined by the ability of the LTA, during its transit through the cell wall, to bind via its polyglycerophosphate backbone or its glycolipid moieties to other constituents of the cytoplasmic membrane and the cell w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.426-433.1982
更新日期:1982-02-01 00:00:00
abstract::During development of the symbiotic soil bacterium Sinorhizobium meliloti into nitrogen-fixing bacteroids, DNA replication and cell division cease and the cells undergo profound metabolic and morphological changes. Regulatory genes controlling the early stages of this process have not been identified. As a first step ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.10.3204-3210.2001
更新日期:2001-05-01 00:00:00
abstract::The essential gene efg, which complements ammonia-dependent growth (adgA) mutations in Rhodobacter capsulatus and is located at 38.1 min on the Escherichia coli chromosome, was found to code for NH3-dependent NAD synthetase. Crude extracts from a strain which overproduces the efg gene product contained up to 400 times...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.11.3400-3402.1994
更新日期:1994-06-01 00:00:00
abstract::T4 gene 2 mutants have a pleiotropic phenotype: degradation of injected phage DNA by exonuclease V (ExoV) in the recBCD(+) host cell cytoplasm and a low burst size due, at least in part, to a decreased ability for head-to-tail (H-T) joining. The more N terminal the mutation, the more pronounced is the H-T joining defe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.3.672-679.2000
更新日期:2000-02-01 00:00:00
abstract::The gamma-proteobacterium Francisella tularensis is one of the most infectious human pathogens, and the highly virulent organism F. tularensis subsp. tularensis (type A) and less virulent organism F. tularensis subsp. holarctica (type B) are most commonly associated with significant disease in humans and animals. Here...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00506-06
更新日期:2006-10-01 00:00:00
abstract::In Escherichia coli, synthesis of sialic acid is not regulated by allosteric inhibition mediated by cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). Evidence for the lack of metabolic control by feedback inhibition was demonstrated by measuring the intracellular level of sialic acid and CMP-NeuNAc in muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.2.854-860.1985
更新日期:1985-11-01 00:00:00
abstract::Several spontaneous cya and crp mutants of Escherichia coli have been selected as clones simultaneously resistant to phage lambda and nalidixic acid and characterized. Both cya and crp mutants have been found to grow as cocci with increased doubling times. They have increased resistance to some mutagens (methylmethane...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.2.545-555.1976
更新日期:1976-02-01 00:00:00
abstract::A colorimetric assay was developed for studying the kinetics of iron oxidation with whole cells of the chemoautotroph, Ferrobacillus ferrooxidans. The assay was more advantageous than the conventional method of Warburg manometry because of its simplicity, rapidity, and the small amount of cells required. The assay mea...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.2.552-557.1969
更新日期:1969-08-01 00:00:00
abstract::CRM 228 (T. Uchida, A. M. Pappenheimer, and R. Greany, J. Biol. Chem. 248:3838-3844, 1973), a mutant form of diphtheria toxin which completely lacks ADP-ribosyltransferase activity, contains five amino acid substitutions. The two amino acid changes that fall within the A chain of the toxin (G79D and E162K) were separa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.15.4766-4769.1994
更新日期:1994-08-01 00:00:00
abstract::phiYeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3. The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6%, and 54 putative genes, which are all transcribed from...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.6.1928-1937.2001
更新日期:2001-03-01 00:00:00
abstract::The positions of the outer boundaries of the 5'- and 3'-conserved segment sequences of integrons found at several different locations have been determined. The position of the 5' end of the 5'-conserved segment is the same for six independently located integrons, In1 (R46), In2 (Tn21), In3 (R388), In4 (Tn1696), In5 (p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.20.6286-6294.1994
更新日期:1994-10-01 00:00:00
abstract::PhoB is the response regulator of the Pho regulon. It is composed of two distinct domains, an N-terminal receiver domain and a C-terminal output domain that binds DNA and interacts with sigma(70) to activate transcription of the Pho regulon. Phosphorylation of the receiver domain is required for activation of the prot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.23.6592-6597.2000
更新日期:2000-12-01 00:00:00
abstract::Escherichia coli mutant N4316 is temperature sensitive and exhibits temperature-dependent suppression. These phenotypes are due to separate genes, as shown by reversion and mapping studies. The suppressor mutation was mapped and lies near argF. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.1141-1143.1977
更新日期:1977-02-01 00:00:00
abstract::Lactococcus lactis subsp. lactis ML3 contains high pools of proline or betaine when grown under conditions of high osmotic strength. These pools are created by specific transport systems. A high-affinity uptake system for glycine betaine (betaine) with a Km of 1.5 microM is expressed constitutively. The activity of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.17.5438-5444.1993
更新日期:1993-09-01 00:00:00
abstract::The activity of bacterial alkaline phosphatase (PhoA) is dependent on it being exported across the plasma membrane. A plasmid vector (pJEM11) allowing fusions between phoA and genes encoding exported proteins was constructed to study protein export in mycobacteria. Introduction of the Mycobacterium fortuitum beta-lact...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.1.59-65.1995
更新日期:1995-01-01 00:00:00