Methylation of the cellular lipid of methionine-requiring Agrobacterium tumefaciens.

abstract：:Tn4551, a clindamycin resistance (Ccr) transposon from the R plasmid pBI136, was cloned onto an Escherichia coli-Bacteroides shuttle vector which could replicate normally in E. coli but was maintained unstably in Bacteroides fragilis. To aid in cloning and to ensure maintenance of Tn4551 in E. coli, a kanamycin resist...

journal_title：Journal of bacteriology

authors： Smith CJ,Spiegel H

更新日期：1987-08-01 00:00:00

abstract：:Strain 4R is a phycocyanin-minus mutant of the unicellular cyanobacterium Synechocystis sp. strain 6803. Although it lacks the light-harvesting protein phycocyanin, 4R has normal levels of phycocyanin (cpc) transcripts. Sequence analysis of the cpcB gene encoding the phycocyanin beta subunit shows an insertion mutatio...

journal_title：Journal of bacteriology

authors： Plank T,Toole C,Anderson LK

更新日期：1995-12-01 00:00:00

abstract：:The growth of tubercle bacilli in serum samples of untreated animals depends upon the availability of ionic iron which serves as a growth factor in supporting bacillary multiplication. The amount of available iron in serum is determined by the ratio between iron-saturated and iron-free transferrin; a low value for the...

journal_title：Journal of bacteriology

authors： Kochan I,Golden CA,Bukovic JA

更新日期：1969-10-01 00:00:00

abstract：:Klebsiella strain RE1544 contains two lac operons, one on the chromosome and one on a lac plasmid. A mutant of RE1544, in which the lacZ genes of both operons produce no active enzyme, was found to synthesize a beta-galactosidase that hydrolyzes ortho-nitrophenyl-beta-D-galactopyranoside but not lactose. Synthesis of ...

journal_title：Journal of bacteriology

authors： Hall BG,Reeve EC

更新日期：1977-10-01 00:00:00

abstract：:Ureaplasma urealyticum cells were lysed by osmotic shock or by digitonin. The membrane fraction contained four to ten times as much protein as the cytoplasmic fraction. These values are in large excess of those reported for classical mycoplasmas, suggesting that the Ureaplasma membrane fraction was heavily contaminate...

journal_title：Journal of bacteriology

authors： Masover GK,Razin S,Hayflick L

更新日期：1977-04-01 00:00:00

abstract：:Integral membrane proteins from over 20 ubiquitous families of channels, secondary carriers, and primary active transporters were analyzed for average size differences between homologues from the three domains of life: Bacteria, Archaea, and Eucarya. The results showed that while eucaryotic homologues are consistently...

journal_title：Journal of bacteriology

authors： Chung YJ,Krueger C,Metzgar D,Saier MH Jr

更新日期：2001-02-01 00:00:00

abstract：:Globomycin is an antibiotic which inhibits the processing of the prolipoprotein. Eighty globomycin-resistant mutants were independently isolated from Escherichia coli K-12 which had a deletion mutation in chromosomal lipoprotein gene (lpp), but contained a plasmid carrying the wild-type lpp gene. Twenty-six of the mut...

journal_title：Journal of bacteriology

authors： Zwiebel LJ,Inukai M,Nakamura K,Inouye M

更新日期：1981-01-01 00:00:00

abstract：:Between 59 and 60 min on the Escherichia coli genetic map, there is a highly conserved sequence of 29 base pairs, containing an inverted repeat of seven base pairs that appears 14 times, 32 or 33 base pairs apart, downstream of the iap gene coding region. About 24 kilobase pairs downstream of the 14 repeats, a similar...

journal_title：Journal of bacteriology

authors： Nakata A,Amemura M,Makino K

更新日期：1989-06-01 00:00:00

abstract：:Pathways of ammonia assimilation into glutamic acid in Bacillus macerans were investigated by measurements of the specific activities of glutamate dehydrogenase (GDH), glutamine synthetase, and glutamate synthase. In ammonia-rich medium, GDH was the predominant pathway of ammonia assimilation. In nitrogen-fixing cells...

journal_title：Journal of bacteriology

authors： Kanamori K,Weiss RL,Roberts JD

更新日期：1987-10-01 00:00:00

abstract：:Anabaenopeptins (AP) are bioactive cyclic hexapeptides synthesized nonribosomally in cyanobacteria. APs are characterized by several conserved motifs, including the ureido bond, N-methylation in position 5, and d-Lys in position 2. All other positions of the AP molecule are variable, resulting in numerous structural v...

journal_title：Journal of bacteriology

authors： Christiansen G,Philmus B,Hemscheidt T,Kurmayer R

更新日期：2011-08-01 00:00:00

abstract：:The general stress regulon of Bacillus subtilis comprises approximately 200 genes and is under the control of the alternative sigma factor σ(B). The activation of σ(B) occurs in response to multiple physical stress stimuli as well as energy starvation conditions. The expression of the general stress proteins provides ...

journal_title：Journal of bacteriology

authors： Reder A,Höper D,Gerth U,Hecker M

更新日期：2012-07-01 00:00:00

abstract：:We describe techniques for isolating individual pairs of mating Escherichia coli and observing them under the light microscope. Some pairs achieved close cell-to-cell contact, whereas others remained loosely connected by invisible connections which may be F pili. After 30 min of mating, the pairs were separated and al...

journal_title：Journal of bacteriology

authors： Ou JT,Anderson TF

更新日期：1970-06-01 00:00:00

abstract：:Polynucleotide phosphorylase (PNP) plays a central role in RNA degradation, generating a pool of ribonucleoside diphosphates (rNDPs) that can be converted to deoxyribonucleoside diphosphates (dNDPs) by ribonucleotide reductase. We report here that spontaneous mutations resulting from replication errors, which are norm...

journal_title：Journal of bacteriology

authors： Becket E,Tse L,Yung M,Cosico A,Miller JH

更新日期：2012-10-01 00:00:00

abstract：:FtsZ, the bacterial tubulin homologue, is the main player in at least two distinct processes of cell division during the development of Streptomyces coelicolor A3(2). It forms cytokinetic rings and is required for the formation of both the widely spaced hyphal cross walls in the substrate mycelium and the specialized ...

journal_title：Journal of bacteriology

authors： Grantcharova N,Lustig U,Flärdh K

更新日期：2005-05-01 00:00:00

abstract：:Some bacteriophages target potentially pathogenic bacteria by exploiting surface-associated virulence factors as receptors. For example, phage have been identified that exhibit specificity for Vi capsule producing Salmonella enterica serovar Typhi. Here we have characterized the Vi-associated E1-typing bacteriophage u...

journal_title：Journal of bacteriology

authors： Pickard D,Thomson NR,Baker S,Wain J,Pardo M,Goulding D,Hamlin N,Choudhary J,Threfall J,Dougan G

更新日期：2008-04-01 00:00:00

abstract：:The growth phase-dependent change in sucrose density gradient centrifugation patterns of ribosomes was analyzed for both laboratory strains of Escherichia coli and natural isolates from the ECOR collection. All of the natural isolates examined formed 100S ribosome dimers in the stationary phase, and ribosome modulatio...

journal_title：Journal of bacteriology

authors： Wada A,Mikkola R,Kurland CG,Ishihama A

更新日期：2000-05-01 00:00:00

abstract：:In order to characterize the genome-wide transcriptional response of the hyperthermophilic, aerobic crenarchaeote Sulfolobus solfataricus to UV damage, we used high-density DNA microarrays which covered 3,368 genetic features encoded on the host genome, as well as the genes of several extrachromosomal genetic elements...

journal_title：Journal of bacteriology

authors： Fröls S,Gordon PM,Panlilio MA,Duggin IG,Bell SD,Sensen CW,Schleper C

更新日期：2007-12-01 00:00:00

abstract：:There are significant differences in the large subunits of form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase isolated from Rhodopseudomonas sphaeroides. Two-dimensional peptide mapping of carboxymethylated large subunits clearly indicates that there are differences in the primary structure of the two ...

journal_title：Journal of bacteriology

authors： Gibson JL,Tabita FR

更新日期：1985-12-01 00:00:00

abstract：:The double-stranded DNA bacteriophage PRD1 uses an IncP plasmid-encoded conjugal transfer complex as a receptor. Plasmid functions in the PRD1 life cycle are restricted to phage adsorption and DNA entry. A single phage structural protein, P2, located at the fivefold capsid vertices, is responsible for PRD1 attachment ...

journal_title：Journal of bacteriology

authors： Grahn AM,Caldentey J,Bamford JK,Bamford DH

更新日期：1999-11-01 00:00:00

abstract：:Eubacterium sp. strain VPI 12708 is an anaerobic intestinal bacterium that has inducible bile acid 7-dehydroxylation activity. At least four new polypeptides were synthesized after addition of primary bile acids to the growth medium. One of these, of molecular weight 27,000 (P-27), was shown to be involved in the 7-de...

journal_title：Journal of bacteriology

authors： Coleman JP,White WB,Lijewski M,Hylemon PB

更新日期：1988-05-01 00:00:00

abstract：:Both fi(+) and fi(-) type R factors function as intact replicative units when segregated into Escherichia coli minicells. Hybridization studies demonstrate that at least 95% of the deoxyribonucleic acid (DNA) in R(+) minicells is episomal in origin. About half of the DNA can be extracted in a closed circular form and ...

journal_title：Journal of bacteriology

authors： Levy SB

更新日期：1971-10-01 00:00:00

abstract：:Anabaena variabilis ATCC 29413 has one Mo nitrogenase that is made under oxic growth conditions in specialized cells called heterocysts and a second Mo nitrogenase that is made only under anoxic conditions in vegetative cells. The two large nif gene clusters responsible for these two nitrogenases are under the control...

journal_title：Journal of bacteriology

authors： Vernon SA,Pratte BS,Thiel T

更新日期：2017-01-30 00:00:00

abstract：:The iron-molybdenum cofactor (FeMo-co) of nitrogenase contains molybdenum, iron, sulfur, and homocitrate in a ratio of 1:7:9:1. In vitro synthesis of FeMo-co has been established, and the reaction requires an ATP-regenerating system, dithionite, molybdate, homocitrate, and at least NifB-co (the metabolic product of Ni...

journal_title：Journal of bacteriology

authors： Shah VK,Rangaraj P,Chatterjee R,Allen RM,Roll JT,Roberts GP,Ludden PW

更新日期：1999-05-01 00:00:00

abstract：:Single spores of Saccharomyces cerevisiae were examined during germination and outgrowth by scanning electron and phase-contrast microscopy. Also determined were changes in cell weight and light absorbance, trehalose utilization, and synthesis of protein and KOH-soluble carbohydrates. These studies reveal that develop...

journal_title：Journal of bacteriology

authors： Rousseau P,Halvorson HO,Bulla LA Jr,St Julian G

更新日期：1972-03-01 00:00:00

abstract：:The ultrastructure of the cytoplasmic membrane and cell wall of two strains of Escherichia coli, Proteus morganii, P. vulgaris, Acinetobacter anitratum, Moraxella lacunata, Erwinia amylovora, Acinetobacter sp., and of a plant pathogen, unclassified gram-negative, fixed by the Ryter-Kellenberger procedure, was found to...

journal_title：Journal of bacteriology

authors： Silva MT,Sousa JC

更新日期：1973-02-01 00:00:00

abstract：:While coagulase-negative staphylococci (CoNS), with their ability to form a thick, multilayered biofilm on foreign bodies, have been identified as the major cause of implant-associated infections, no data are available about biofilm formation by staphylococcal small-colony variants (SCVs). In the past years, a number ...

journal_title：Journal of bacteriology

authors： Al Laham N,Rohde H,Sander G,Fischer A,Hussain M,Heilmann C,Mack D,Proctor R,Peters G,Becker K,von Eiff C

更新日期：2007-06-01 00:00:00

abstract：:Western blot (immunoblot) analysis of cell extracts from induced bacteriophage lambda lysogens probed with S-protein-specific antibody (raised against an S--beta-galactosidase fusion protein) demonstrated that the bacteriophage lambda S protein begins to appear 10 min after phage induction and is localized to the inne...

journal_title：Journal of bacteriology

authors： Zagotta MT,Wilson DB

更新日期：1990-02-01 00:00:00

abstract：:An examination of the methanofurans isolated from a wide range of methanogenic bacteria and from Archaeoglobus fulgidus has revealed at least five chromatographically distinct methanofurans. Bacteria from each major genus of methanogenic bacteria have been found to contain a chemically different methanofuran. The natu...

journal_title：Journal of bacteriology

authors： White RH

更新日期：1988-10-01 00:00:00

abstract：:Pep 5 and nisin are cationic peptide antibiotics which in addition to their membrane-disruptive action induce autolysis in staphylococci. To investigate the mechanism of lysis induction, the influence of the peptides on the activity of the N-acetylmuramoyl-L-alanine amidase of Staphylococcus simulans 22 was studied. I...

journal_title：Journal of bacteriology

authors： Bierbaum G,Sahl HG

更新日期：1987-12-01 00:00:00

abstract：:Cell survival, deoxyribonucleic acid (DNA) degradation, and the repair of DNA single-strand breaks were measured for Escherichia coli K-12 pol+, polA1, polC1026(ts), and polA1 polC1026(ts) cells after 137Cs gamma irradiation. The results indicate that DNA polymerase III is required for growth medium-dependent (type II...

journal_title：Journal of bacteriology

authors： Hamelin C,Youngs DA,Smith KC

更新日期：1976-09-01 00:00:00