Bacteriophage T4 self-assembly: in vitro reconstitution of recombinant gp2 into infectious phage.

Abstract:

:T4 gene 2 mutants have a pleiotropic phenotype: degradation of injected phage DNA by exonuclease V (ExoV) in the recBCD(+) host cell cytoplasm and a low burst size due, at least in part, to a decreased ability for head-to-tail (H-T) joining. The more N terminal the mutation, the more pronounced is the H-T joining defect. We have overexpressed and purified the recombinant gene 2 product (rgp2) to homogeneity in order to test its role in H-T joining, during in vitro reconstitution. When we mix extracts of heads from a gp2(+) phage infection (H(+)) with tails from a gp2(+) or gp2(-) phage infection (T(+) or T(-)), the H-T joining is fast and all of the reconstituted phage grow equally well on cells with or without ExoV activity. When heads from gene 2 amber mutants (H(-)) are used, addition of rgp2 is required for H-T joining. In this case, H-T joining is slow and only about 10% of the reconstituted phage can form plaques on ExoV(+) cells. When extracts of heads with different gene 2 amber mutations are mixed with extracts of tails (with a gene 2 amber mutation) in the presence of rgp2, we find that the size of the gp2 amber peptide of the head extract is inversely related to the fraction of reconstituted phage with a 2(+) phenotype. We conclude that free rgp2 is biologically active and has a direct role in H-T joining but that the process is different from H-T joining promoted by natural gp2 that is incorporated into the head in vivo. Furthermore, it seems that gp2 has a domain which binds it to the head. Thus, the presence of the longer gp2am mutants (with this domain) inhibits their replacement by full-length rgp2.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Wang GR,Vianelli A,Goldberg EB

doi

10.1128/jb.182.3.672-679.2000

keywords:

subject

Has Abstract

pub_date

2000-02-01 00:00:00

pages

672-9

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

182

pub_type

杂志文章
  • Integrative genomic, transcriptional, and proteomic diversity in natural isolates of the human pathogen Burkholderia pseudomallei.

    abstract::Natural isolates of pathogenic bacteria can exhibit a broad range of phenotypic traits. To investigate the molecular mechanisms contributing to such phenotypic variability, we compared the genomes, transcriptomes, and proteomes of two natural isolates of the gram-negative bacterium Burkholderia pseudomallei, the causa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.12.4276-4285.2005

    authors: Ou K,Ong C,Koh SY,Rodrigues F,Sim SH,Wong D,Ooi CH,Ng KC,Jikuya H,Yau CC,Soon SY,Kesuma D,Lee MA,Tan P

    更新日期:2005-06-01 00:00:00

  • Different roles for KinA, KinB, and KinC in the initiation of sporulation in Bacillus subtilis.

    abstract::Activation (phosphorylation) of the transcription factor encoded by spo0A is essential for the initiation of sporulation in Bacillus subtilis. At least three histidine protein kinases are involved in the phosphorylation of Spo0A. Under some growth conditions, KinA was the primary kinase, but under other conditions, Ki...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.3.861-863.1995

    authors: LeDeaux JR,Yu N,Grossman AD

    更新日期:1995-02-01 00:00:00

  • Topological model of the Rhodobacter capsulatus light-harvesting complex I assembly protein LhaA (previously known as ORF1696).

    abstract::A theoretical topology of the Rhodobacter capsulatus membrane protein LhaA was formulated and evaluated by gene fusion experiments. The apparent topological locations of fusion enzymes were compared with the theoretically derived structure, and a model of LhaA is suggested that consists of 12 transmembrane segments, w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.17.4742-4745.1998

    authors: Young CS,Beatty JT

    更新日期:1998-09-01 00:00:00

  • Genetic analysis of antibiotic resistance in Streptococcus pyogenes.

    abstract::The genetics of antibiotic resistance in mutant strains of Streptococcus pyrogenes was studied. Utilizing a type 6 strain (9440) primarily resistant to strepttomycin (Strr), classes of mutant strains were isolated that were resistant to one of the following antibiotics: rifampin (Rifr), erythromycin (Eryr), thiostrept...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.2.852-859.1978

    authors: Stuart JG,Ferretti JJ

    更新日期:1978-02-01 00:00:00

  • Origins of the 2,4-dinitrotoluene pathway.

    abstract::The degradation of synthetic compounds requires bacteria to recruit and adapt enzymes from pathways for naturally occurring compounds. Previous work defined the steps in 2,4-dinitrotoluene (2,4-DNT) metabolism through the ring fission reaction. The results presented here characterize subsequent steps in the pathway th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.15.4219-4232.2002

    authors: Johnson GR,Jain RK,Spain JC

    更新日期:2002-08-01 00:00:00

  • Recombination in the ompA gene but not the omcB gene of Chlamydia contributes to serovar-specific differences in tissue tropism, immune surveillance, and persistence of the organism.

    abstract::Sequences of the major outer membrane protein (MOMP) gene (ompA) and the outer membrane complex B protein gene (omcB) from Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydia psittaci were analyzed for evidence of intragenic recombination and for linkage equilibrium. The Sawyer runs test, compatibility matrices...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.20.5997-6008.2001

    authors: Millman KL,Tavaré S,Dean D

    更新日期:2001-10-01 00:00:00

  • complex interplay between type 1 fimbrial expression and flagellum-mediated motility of uropathogenic Escherichia coli.

    abstract::Type 1 fimbriae and flagella have been previously shown to contribute to the virulence of uropathogenic Escherichia coli (UPEC) within the urinary tract. In this study, the relationship between motility and type 1 fimbrial expression was tested for UPEC strain CFT073 by examining the phenotypic effect of fimbrial expr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00434-07

    authors: Lane MC,Simms AN,Mobley HL

    更新日期:2007-08-01 00:00:00

  • Molecular cloning, expression, and characterization of a Ca2+-dependent, membrane-associated nuclease of Mycoplasma genitalium.

    abstract::In this study, we identified and characterized the enzymatic properties of MG_186, a calcium-dependent Mycoplasma genitalium nuclease. MG_186 displays the hallmarks of nucleases, as indicated by its amino acid sequence similarity to other nucleases. We cloned, UGA corrected, expressed, purified, and demonstrated that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00401-10

    authors: Li L,Krishnan M,Baseman JB,Kannan TR

    更新日期:2010-10-01 00:00:00

  • Alanine dehydrogenase (ald) is required for normal sporulation in Bacillus subtilis.

    abstract::The ski22::Tn917lac insertion mutation in Bacillus subtilis was isolated in a screen for mutations that cause a defect in sporulation but are suppressed by the presence or overexpression of the histidine protein kinase encoded by kinA (spoIIJ). The ski22::Tn917lac insertion mutation was in ald, the gene encoding alani...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.21.6789-6796.1993

    authors: Siranosian KJ,Ireton K,Grossman AD

    更新日期:1993-11-01 00:00:00

  • Compartmentalization of the periplasmic space at division sites in gram-negative bacteria.

    abstract::Phase-contrast and serial-section electron microscopy were used to study the patterns of localized plasmolysis that occur when cells of Salmonella typhimurium and Escherichia coli are exposed to hypertonic solutions of sucrose. In dividing cells the nascent septum was flanked by localized regions of periseptal plasmol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.3.1430-1438.1986

    authors: Cook WR,MacAlister TJ,Rothfield LI

    更新日期:1986-12-01 00:00:00

  • Phenotypic and genotypic evidence for L-fucose utilization by Campylobacter jejuni.

    abstract::Campylobacter jejuni remains among the leading causes of bacterial food-borne illness. The current understanding of Campylobacter physiology suggests that it is asaccharolytic and is unable to catabolize exogenous carbohydrates. Contrary to this paradigm, we provide evidence for l-fucose utilization by C. jejuni. The ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01252-10

    authors: Muraoka WT,Zhang Q

    更新日期:2011-03-01 00:00:00

  • Phosphatidylglycerolphosphate synthase expression in Schizosaccharomyces pombe is regulated by the phospholipid precursors inositol and choline.

    abstract::The enzyme phosphatidylglycerolphosphate synthase (PGPS; CDP-diacylglycerol glycerol 3-phosphate 3-phosphatidyltransferase; EC 2.7.8.5) catalyzes the committed step in the cardiolipin biosynthetic pathway. To study the regulation of PGPS in Schizosaccharomyces pombe, we characterized the enzyme biochemically. Maximum ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.19.6132-6138.1991

    authors: Karkhoff-Schweizer RR,Kelly BL,Greenberg ML

    更新日期:1991-10-01 00:00:00

  • Mutational analysis of flagellum-independent surface spreading of Serratia marcescens 274 on a low-agar medium.

    abstract::In a previous study (J. O'Rear, L. Alberti, and R. M. Harshey, J. Bacteriol. 174:6125-6137, 1992) we reported the isolation of several transposon mutants of Serratia marcescens 274 that were defective either in swarming alone or in both swimming and swarming motility. All the nonflagellate (Fla-) mutants, while defect...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.4.987-991.1995

    authors: Matsuyama T,Bhasin A,Harshey RM

    更新日期:1995-02-01 00:00:00

  • Mutations to spectinomycin resistance which alleviate the restriction of an amber suppressor by streptomycin resistance.

    abstract::Unlike mutations to streptomycin resistance, which restrict the function of an SU II amber suppressor, mutations to spectinomycin resistance have no restrictive effect. Furthermore, mutations to spectinomycin resistance counteract the restrictive action of streptomycin-resistant mutations when combined in a single str...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.2.940-943.1969

    authors: Kuwano M,Endo H,Ohnishi Y

    更新日期:1969-02-01 00:00:00

  • Dinitrogenase reductase-activating glycohydrolase can be released from chromatophores of Rhodospirillum rubrum by treatment with MgGDP.

    abstract::Dinitrogenase reductase-activating glycohydrolase (DRAG), involved in the regulation of nitrogenase activity in Rhodospirillum rubrum, is associated with chromatophore membranes in cell extracts. We show that DRAG can be specifically released by treatment with MgGDP; other nucleotides studied had no effect. The DRAG a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.24.7872-7874.1997

    authors: Norén A,Nordlund S

    更新日期:1997-12-01 00:00:00

  • Impact of phosphorylation of specific residues in the tyrosine autokinase, Wzc, on its activity in assembly of group 1 capsules in Escherichia coli.

    abstract::Wzc(CPS) is a tyrosine autokinase essential for the assembly of a high-molecular-weight (HMW) group 1 capsular polysaccharide (CPS) in Escherichia coli. Homologues of Wzc participate in the formation of CPS and exopolysaccharides in a variety of gram-positive and gram-negative bacteria. Phosphorylation of tyrosine res...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.23.6437-6447.2002

    authors: Paiment A,Hocking J,Whitfield C

    更新日期:2002-12-01 00:00:00

  • Medium-dependent alteration of lysine transfer ribonucleic acid in sporulating Bacillus subtilis cells.

    abstract::The presence of the altered lysine transfer ribonucleic acid (tRNA) in Bacillus subtilis spores is strongly dependent on the medium on which the cells were sporulated. Cells sporulated on synthetic media or dilute complex media contain little or none of the new component, whereas those sporulated on concentrated compl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.1.125-130.1967

    authors: Lazzarini RA,Santangelo E

    更新日期:1967-07-01 00:00:00

  • Characterization of the role of the ToxR-modulated outer membrane porins OmpU and OmpT in Vibrio cholerae virulence.

    abstract::ToxR, the transmembrane regulatory protein required for expression of virulence factors in the human diarrheal pathogen Vibrio cholerae, directly activates and represses the transcription of two outer membrane porins, OmpU and OmpT, respectively. In an attempt to dissect the role of the OmpU and OmpT porins in viabili...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.12.3652-3662.2001

    authors: Provenzano D,Lauriano CM,Klose KE

    更新日期:2001-06-01 00:00:00

  • Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in Escherichia coli by a virD-encoded border-specific endonuclease from Agrobacterium tumefaciens.

    abstract::The virD locus of Agrobacterium tumefaciens Ti plasmid pTiA6 was sequenced. Computer analysis of the sequence indicated five possible open reading frames (ORFs) within this locus. Two additional ORFs were identified distal to this locus. However, only two polypeptides of apparent molecular masses 16 and 56 kilodaltons...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.11.5035-5045.1987

    authors: Jayaswal RK,Veluthambi K,Gelvin SB,Slightom JL

    更新日期:1987-11-01 00:00:00

  • Analysis of the hypervariable region of the Salmonella enterica genome associated with tRNA(leuX).

    abstract::The divergence of Salmonella enterica and Escherichia coli is estimated to have occurred approximately 140 million years ago. Despite this evolutionary distance, the genomes of these two species still share extensive synteny and homology. However, there are significant differences between the two species in terms of g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.7.2469-2482.2005

    authors: Bishop AL,Baker S,Jenks S,Fookes M,Gaora PO,Pickard D,Anjum M,Farrar J,Hien TT,Ivens A,Dougan G

    更新日期:2005-04-01 00:00:00

  • Effect of mycosuppressin on the respiration and growth of Mycobacterium tuberculosis.

    abstract::Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.) and Guy P. Youmans. Effect of mycosuppressin on the respiration and growth of Mycobacterium tuberculosis. J. Bacteriol. 84:708-715. 1962.-A substance, called mycosuppressin, was found in the lungs of guinea pigs and rabbits vaccinated with BCG or...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.4.708-715.1962

    authors: YOUMANS AS,YOUMANS GP

    更新日期:1962-10-01 00:00:00

  • Interaction of native and mutant MecI repressors with sequences that regulate mecA, the gene encoding penicillin binding protein 2a in methicillin-resistant staphylococci.

    abstract::Methicillin resistance in staphylococci is mediated by PBP2a, a penicillin binding protein with low affinity for beta-lactam antibiotics. The gene encoding PBP2a, mecA, is transcriptionally regulated in some clinical isolates by mecR1 and mecI, genes divergently transcribed from mecA that encode a signal transducer an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.8.2160-2166.1998

    authors: Sharma VK,Hackbarth CJ,Dickinson TM,Archer GL

    更新日期:1998-04-01 00:00:00

  • Fusion of yeast spheroplasts.

    abstract::Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions. After reversion to vegetative cells in solid media, the resulting zygotes were shown to be diploid cells of mating type aa. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.2.946-947.1977

    authors: van Solingen P,van der Plaat JB

    更新日期:1977-05-01 00:00:00

  • Genomic analysis reveals chromosomal variation in natural populations of the uncultured psychrophilic archaeon Cenarchaeum symbiosum.

    abstract::Molecular phylogenetic surveys have recently revealed an ecologically widespread crenarchaeal group that inhabits cold and temperate terrestrial and marine environments. To date these organisms have resisted isolation in pure culture, and so their phenotypic and genotypic characteristics remain largely unknown. To cha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.19.5003-5009.1998

    authors: Schleper C,DeLong EF,Preston CM,Feldman RA,Wu KY,Swanson RV

    更新日期:1998-10-01 00:00:00

  • Molecular, genetic, and biochemical characterization of the serC gene of Methanosarcina barkeri Fusaro.

    abstract::The Methanosarcina barkeri serC gene, encoding phosphoserine aminotransferase, was cloned by complementation of an Escherichia coli serC mutant, and its nucleotide sequence was determined. The M. barkeri SerC protein shares significant homology with other known SerC proteins. E. coli serC hosts carrying the cloned gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.19.5797-5802.1996

    authors: Metcalf WW,Zhang JK,Shi X,Wolfe RS

    更新日期:1996-10-01 00:00:00

  • Proteome analysis of metabolically engineered Escherichia coli producing Poly(3-hydroxybutyrate).

    abstract::Recombinant Escherichia coli strains harboring heterologous polyhydroxyalkanoate (PHA) biosynthesis genes were shown to accumulate unusually large amounts of PHA. In the present study, integrated cellular responses of metabolically engineered E. coli to the accumulation of poly(3-hydroxybutyrate) (PHB) in the early st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.1.301-308.2001

    authors: Han MJ,Yoon SS,Lee SY

    更新日期:2001-01-01 00:00:00

  • Escherichia coli NsrR regulates a pathway for the oxidation of 3-nitrotyramine to 4-hydroxy-3-nitrophenylacetate.

    abstract::Chromatin immunoprecipitation and microarray (ChIP-chip) analysis showed that the nitric oxide (NO)-sensitive repressor NsrR from Escherichia coli binds in vivo to the promoters of the tynA and feaB genes. These genes encode the first two enzymes of a pathway that is required for the catabolism of phenylethylamine (PE...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00508-08

    authors: Rankin LD,Bodenmiller DM,Partridge JD,Nishino SF,Spain JC,Spiro S

    更新日期:2008-09-01 00:00:00

  • Functional characterization of seven γ-Glutamylpolyamine synthetase genes and the bauRABCD locus for polyamine and β-Alanine utilization in Pseudomonas aeruginosa PAO1.

    abstract::Pseudomonas aeruginosa and many other bacteria can utilize biogenic polyamines, including diaminopropane (DAP), putrescine (Put), cadaverine (Cad), and spermidine (Spd), as carbon and/or nitrogen sources. Transcriptome analysis in response to exogenous Put and Spd led to the identification of a list of genes encoding ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05105-11

    authors: Yao X,He W,Lu CD

    更新日期:2011-08-01 00:00:00

  • Mfd is required for rapid recovery of transcription following UV-induced DNA damage but not oxidative DNA damage in Escherichia coli.

    abstract::Transcription-coupled repair (TCR) is a cellular process by which some forms of DNA damage are repaired more rapidly from transcribed strands of active genes than from nontranscribed strands or the overall genome. In humans, the TCR coupling factor, CSB, plays a critical role in restoring transcription following both ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06725-11

    authors: Schalow BJ,Courcelle CT,Courcelle J

    更新日期:2012-05-01 00:00:00

  • cobB function is required for catabolism of propionate in Salmonella typhimurium LT2: evidence for existence of a substitute function for CobB within the 1,2-propanediol utilization (pdu) operon.

    abstract::The cobB function of Salmonella typhimurium LT2 was defined in vivo as an alternative activity for the nicotinic acid mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase enzyme (CobT), which is involved in the assembly of the nucleotide loop of cobalamin in this bacterium (J. R. Trzebiatowski, G. A. O'T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.23.7016-7019.1996

    authors: Tsang AW,Escalante-Semerena JC

    更新日期:1996-12-01 00:00:00