Abstract:
:A chemotaxis signal transduction pathway (hereafter called Che1) has been previously identified in the alphaproteobacterium Azospirillum brasilense. Previous experiments have demonstrated that although mutants lacking CheB and/or CheR homologs from this pathway are defective in chemotaxis, a mutant in which the entire chemotaxis pathway has been mutated displayed a chemotaxis phenotype mostly similar to that of the parent strain, suggesting that the primary function of this Che1 pathway is not the control of motility behavior. Here, we report that mutants carrying defined mutations in the cheA1 (strain AB101) and the cheY1 (strain AB102) genes and a newly constructed mutant lacking the entire operon [Delta(cheA1-cheR1)::Cm] (strain AB103) were defective, but not null, for chemotaxis and aerotaxis and had a minor defect in swimming pattern. We found that mutations in genes of the Che1 pathway affected the cell length of actively growing cells but not their growth rate. Cells of a mutant lacking functional cheB1 and cheR1 genes (strain BS104) were significantly longer than wild-type cells, whereas cells of mutants impaired in the cheA1 or cheY1 genes, as well as a mutant lacking a functional Che1 pathway, were significantly shorter than wild-type cells. Both the modest chemotaxis defects and the observed differences in cell length could be complemented by expressing the wild-type genes from a plasmid. In addition, under conditions of high aeration, cells of mutants lacking functional cheA1 or cheY1 genes or the Che1 operon formed clumps due to cell-to-cell aggregation, whereas the mutant lacking functional CheB1 and CheR1 (BS104) clumped poorly, if at all. Further analysis suggested that the nature of the exopolysaccharide produced, rather than the amount, may be involved in this behavior. Interestingly, mutants that displayed clumping behavior (lacking cheA1 or cheY1 genes or the Che1 operon) also flocculated earlier and quantitatively more than the wild-type cells, whereas the mutant lacking both CheB1 and CheR1 was delayed in flocculation. We propose that the Che1 chemotaxis-like pathway modulates the cell length as well as clumping behavior, suggesting a link between these two processes. Our data are consistent with a model in which the function of the Che1 pathway in regulating these cellular functions directly affects flocculation, a cellular differentiation process initiated under conditions of nutritional imbalance.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Bible AN,Stephens BB,Ortega DR,Xie Z,Alexandre Gdoi
10.1128/JB.00734-08subject
Has Abstractpub_date
2008-10-01 00:00:00pages
6365-75issue
19eissn
0021-9193issn
1098-5530pii
JB.00734-08journal_volume
190pub_type
杂志文章abstract::The transport of the tricarboxylic acid cycle C(4)-dicarboxylic acids was studied in both the wild-type strain and tricarboxylic acid cycle mutants of Bacillus subtilis. Active transport of malate, fumarate, and succinate was found to be inducible by these dicarboxylic acids or by precursors to them, whereas glucose o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.1.65-79.1973
更新日期:1973-04-01 00:00:00
abstract::Phosphopantetheine adenylyltransferase (PPAT) from Escherichia coli is an essential hexameric enzyme that catalyzes the penultimate step in coenzyme A (CoA) biosynthesis and is a target for antibacterial drug discovery. The enzyme utilizes Mg-ATP and phosphopantetheine (PhP) to generate dephospho-CoA (dPCoA) and pyrop...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00732-07
更新日期:2007-11-01 00:00:00
abstract::A strain of Veillonella parvula M4, which grows readily in lactate broth without a requirement for carbon dioxide, has been isolated from the oral cavity. Anaerobic, washed cells of this organism fermented sodium lactate to the following products (moles/100 moles of lactate): propionate, 66; acetate, 40; carbon dioxid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.999-1005.1971
更新日期:1971-03-01 00:00:00
abstract::Circulating endotoxin was specifically precipitated from plasma samples withdrawn from three different animal species subsequent to parenteral injection of the toxin. Lipoprotein-positive staining and esterase activity were demonstrated on the precipitation lines formed in immunodiffusion, thus establishing the in viv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.6.2031-2034.1968
更新日期:1968-06-01 00:00:00
abstract::Both alpha-tocopherolquinol and alpha-tocopherolquinone were found in 56 of 93 strains of microorganisms examined. Organisms that contained these compounds included the single example of a eucaryotic alga, a Euglena, and a cyanobacterium (blue-green alga), 22 of 32 genera of bacteria, and 9 genera of yeasts. In the ba...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.3.1397-1402.1982
更新日期:1982-09-01 00:00:00
abstract::Lysates of Escherichia coli Ymel obtained from cultures grown in the absence of tryptophan in minimal medium supplemented with 0.1% casein hydrolysate show an approximate fivefold increase in steady-state specific activity of both anthranilate synthetase and tryptophan synthetase A protein relative to cultures grown i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.3.1181-1191.1971
更新日期:1971-12-01 00:00:00
abstract::Lee, Spencer H. S. (Dalhousie University, Halifax, Nova Scotia). Effect of 6-aminonicotinamide on chick-embryo tissue cultures infected with vaccinia virus. J. Bacteriol. 88:885-892. 1964.-6-Aminonicotinamide (6-AN) was shown to render chick-embryo fibroblast (CEF) tissue cultures refractory to infection with vaccinia...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.4.885-892.1964
更新日期:1964-10-01 00:00:00
abstract::The Aer protein in Escherichia coli is a membrane-bound, FAD-containing aerotaxis and energy sensor that putatively monitors the redox state of the electron transport system. Binding of FAD to Aer requires the N-terminal PAS domain and residues in the F1 region and C-terminal HAMP domain. The PAS domains of other PAS ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.20.6782-6791.2004
更新日期:2004-10-01 00:00:00
abstract::The aerobic respiratory chain of Escherichia coli can function with either of two different membrane-bound NADH dehydrogenases (NDH-1 and NDH-2) and with either of two ubiquinol oxidases (bd-type and bo-type). The amounts of each of these enzymes present in the E. coli membrane depend on growth conditions in general a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.10.3020-3025.1993
更新日期:1993-05-01 00:00:00
abstract::A general, genetic technique for determining the direction of transcription for bacterial genes is presented. By comparing the phenotype of Mu-Mud1 dilysogens with the phenotype of deletion-containing derivatives, the direction of transcription for the gene containing Mud1 can be unambiguously determined. This method ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.1.260-268.1981
更新日期:1981-04-01 00:00:00
abstract::The conjugally acquired deoxyribonucleic acid (DNA) of small, anucleate cells ("minicells") of a mutant strain of Escherichia coli K-12 was found to be predominantly associated with the bacterial membrane. Evidence from X-irradiation studies in vivo shows that there is no decrease in DNA-membrane association under con...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.2.626-633.1971
更新日期:1971-05-01 00:00:00
abstract::A cell-free crude extract of Erysipelothrix rhusiopathiae injected by high pressure jet into the knee-joint of rabbits stimulated an acute, mild inflammatory reaction. Additional injections at 3-day intervals induced a chronic condition characterized by hyperplasia of the synovial cells and hypertrophy of the villi, d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.2.403-406.1969
更新日期:1969-05-01 00:00:00
abstract::A mutator mutation of Salmonella typhimurium, observed in a strain resistant to P22 and rifampin, affects mutation rates of bacterial, R factor, and prophage P22 genes. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.2.638-639.1974
更新日期:1974-08-01 00:00:00
abstract::Converse, John L. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), Merida W. Castleberry, and Ernest M. Snyder. Experimental viable vaccine against pulmonary coccidioidomycosis in monkeys. J. Bacteriol. 86:1041-1051. 1963.-Monkeys (Macaca mulatta) vaccinated by subcutaneous injection in the forearm w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.5.1041-1051.1963
更新日期:1963-11-01 00:00:00
abstract::Bacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.18.4952-4961.2002
更新日期:2002-09-01 00:00:00
abstract::Arai, Tadashi (Chiba University, Chiba, Japan), Shyuko Kuroda, and Michio Ito. Possible utility of a fluorescent antibody technique in the serological identification of antagonistic Streptomyces. J. Bacteriol. 83:20-26. 1962.-The indirect fluorescent antibody technique was extended to the serological investigation of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.1.20-26.1962
更新日期:1962-01-01 00:00:00
abstract::The binding of Bacteroides intermedius VPI 8944 to human fibrinogen has been characterized. The binding is time dependent, at least partially reversible, saturable, and specific. On an average, a maximum of 3,500 fibrinogen molecules bind per bacterial cell, with a dissociation constant of 1.7 X 10(-11) M. These bacte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.2.623-628.1985
更新日期:1985-08-01 00:00:00
abstract::The Bacillus anthracis endospore loses resistance properties during germination when its cortex peptidoglycan is degraded by germination-specific lytic enzymes (GSLEs). Although this event normally employs several GSLEs for complete cortex removal, the SleB protein alone can facilitate enough cortex hydrolysis to prod...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00869-10
更新日期:2011-01-01 00:00:00
abstract::The transglycosylation step of cell wall synthesis is a prime antibiotic target because it is essential and specific to bacteria. Two antibiotics, ramoplanin and moenomycin, target this step by binding to the substrate lipid II and the transglycosylase enzyme, respectively. Here, we compare the ramoplanin and moenomyc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05862-11
更新日期:2011-10-01 00:00:00
abstract::Agrobacterium tumefaciens A281 induces large, rapidly appearing tumors on a variety of plants and has a wider host range than other strains of A. tumefaciens. By using Tn3HoHo1 transposon mutagenesis and complementation analysis, a 2.5-kilobase DNA fragment which is responsible for the supervirulence phenotype was ide...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.10.4417-4425.1987
更新日期:1987-10-01 00:00:00
abstract::In the filamentous cyanobacterium Anabaena variabilis, specialized cells called heterocysts occur in a regular pattern along the filament and are the sites of nitrogen fixation. We used two different types of DNA-excess RNA hybridization techniques to estimate the number of genes expressed in recently differentiated, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.3.940-946.1986
更新日期:1986-09-01 00:00:00
abstract::The cytochrome c(3) of Desulfovibrio desulfuricans and that of D. vulgaris were purified to homogeneity as judged by disc gel electrophoresis and by ultracentrifugation. Both cytochromes had an oxidation-reduction potential of -205 +/- 5 mv at pH 7.0 and showed characteristic absorption bands at 525 and 553 nm in the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.358-364.1969
更新日期:1969-10-01 00:00:00
abstract::The citrate transporter CitP of lactic acid bacteria catalyzes electrogenic precursor-product exchange of citrate versus L-lactate during citrate-glucose cometabolism. In the absence of sugar, L-lactate is replaced by the metabolic intermediates/end products pyruvate, α-acetolactate, and acetate. In this study, the bi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00196-12
更新日期:2012-07-01 00:00:00
abstract::Protocatechuate 3,4-dioxygenase is a member of a family of bacterial enzymes that cleave the aromatic rings of their substrates between two adjacent hydroxyl groups, a key reaction in microbial metabolism of varied environmental chemicals. In an appropriate genetic background, it is possible to select for Acinetobacte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.20.6478-6487.1999
更新日期:1999-10-01 00:00:00
abstract::Evidence is presented that progametangia in both the plus and the minus mating types of Mucor mucedo can be induced by one substance, namely (-)-trisporic acid B. A method is described for the determination of the concentration of the sex factors (trisporone, trisporic acid B, trisporic acid C) in mated cultures of Mu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.2.423-428.1970
更新日期:1970-02-01 00:00:00
abstract::The effects of bdellovibrio infection on host permeability and respiration were investigated by measuring respiration rates and the rate of o-nitrophenyl-beta-d-galactopyranoside hydrolysis during the course of single infection cycles of Bdellovibrio bacteriovorus strain 109 growing on Escherichia coli ML 35 (lac i(-)...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.1.149-160.1970
更新日期:1970-04-01 00:00:00
abstract::The stator proteins PomA and PomB form a complex that couples Na(+) influx to torque generation in the polar flagellar motor of Vibrio alginolyticus. This stator complex is anchored to an appropriate place around the rotor through a putative peptidoglycan-binding (PGB) domain in the periplasmic region of PomB (PomB(C)...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00113-11
更新日期:2011-08-01 00:00:00
abstract::Cytotoxicity is an important virulence determinant in the pathogenesis of Vibrio vulnificus, and two cytotoxins, RTX (encoded by rtxA1) and cytolysin/hemolysin (encoded by vvhA), have been identified in this organism. We showed that the quorum-sensing regulator LuxO controlled the cytotoxicity of this organism: a Δlux...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01259-10
更新日期:2011-05-01 00:00:00
abstract::The photoautotrophic cyanobacterium Anacystis nidulans was used to investigate the membrane transport of branched-chain, neutral amino acids and its dependence on photosynthetic reactions. The uptake of alpha-amino [1-14C]isobutyric acid and L-[1-14C]leucine followed Michaelis, Menten kinetics and resulted in an energ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.229-236.1982
更新日期:1982-07-01 00:00:00
abstract:UNLABELLED:Edwardsiella tarda is an important pathogenic bacterium that can replicate in macrophages. However, how the intramacrophage infection process affects the virulence of this bacterium is essentially unknown. Here, we show that E. tarda replicates and induces a caspase-1-dependent cell pyroptosis in a murine ma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00978-15
更新日期:2016-04-28 00:00:00