Phosphopantetheine adenylyltransferase from Escherichia coli: investigation of the kinetic mechanism and role in regulation of coenzyme A biosynthesis.

abstract：:In this study, we identified and characterized the enzymatic properties of MG_186, a calcium-dependent Mycoplasma genitalium nuclease. MG_186 displays the hallmarks of nucleases, as indicated by its amino acid sequence similarity to other nucleases. We cloned, UGA corrected, expressed, purified, and demonstrated that ...

journal_title：Journal of bacteriology

authors： Li L,Krishnan M,Baseman JB,Kannan TR

更新日期：2010-10-01 00:00:00

abstract：:The mannans of Saccharomyces chevalieri, S. italicus, S. diastaticus, and S. carlsbergensis, were acetolyzed, and the fragments were separated by gel filtration. All gave similar acetolysis fingerprints, which were distinguished from S. cerevisiae by the presence of a pentasaccharide component in addition to the mono-...

journal_title：Journal of bacteriology

authors： Ballou CE,Lipke PN,Raschke WC

更新日期：1974-02-01 00:00:00

abstract：:Genome sequencing revealed that all six chlamydiae genomes contain three groEL-like genes (groEL1, groEL2, and groEL3). Phylogenetic analysis of groEL1, groEL2, and groEL3 indicates that these genes are likely to have been present in chlamydiae since the beginning of the lineage. Comparison of deduced amino acid seque...

journal_title：Journal of bacteriology

authors： Karunakaran KP,Noguchi Y,Read TD,Cherkasov A,Kwee J,Shen C,Nelson CC,Brunham RC

更新日期：2003-03-01 00:00:00

abstract：:The Haemophilus cryptic genospecies is an important cause of maternal genital tract and neonatal systemic infections and initiates infection by colonizing the genital or respiratory epithelium. In recent work, we identified a unique Haemophilus cryptic genospecies protein called Cha, which mediates efficient adherence...

journal_title：Journal of bacteriology

authors： Sheets AJ,St Geme JW 3rd

更新日期：2011-01-01 00:00:00

abstract：:The invasion of intestinal epithelial cells by Salmonella enterica serovar Typhimurium is mediated by a type III secretion system (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). Expression of the SPI1 T3SS is tightly regulated by the combined action of HilC, HilD, and RtsA, three AraC family members that c...

journal_title：Journal of bacteriology

authors： Ellermeier JR,Slauch JM

更新日期：2008-01-01 00:00:00

abstract：:When the crude ribosomal fraction of Saccharomyces cerevisiae was separated into "light" and "heavy" fractions, fatty acid synthetase was concentrated in the former, whereas acetyl-Coenzyme A synthetase, fatty acid "desaturase," and squalene oxidocyclase were found in the latter. The "desaturase" sedimented with the r...

journal_title：Journal of bacteriology

authors： Klein HP,Volkmann CM,Leaffer MA

更新日期：1967-07-01 00:00:00

abstract：:Forty-nine human and environmental isolates of Mycobacterium intracellulare and Mycobacterium scrofulaceum were tested for their ability to grow on uric acid and a number of its degradation products. Nearly all (88 to 90%) strains used uric acid or allantoin as a sole nitrogen source; fewer (47 to 69%) used allantoate...

journal_title：Journal of bacteriology

authors： Falkinham JO 3rd,George KL,Parker BC,Gruft H

更新日期：1983-07-01 00:00:00

abstract：:A bacterium was isolated by elective culture with p-hydroxybenzoate as substrate and nitrate as electron acceptor. It grew either aerobically or anaerobically, by nitrate respiration, on a range of aromatic compounds. The organism was identified as a pseudomonad and was given the trivial name Pseudomonas PN-1. Benzoat...

journal_title：Journal of bacteriology

authors： Taylor BF,Campbell WL,Chinoy I

更新日期：1970-05-01 00:00:00

abstract：:Growth of Escherichia coli B in simple media enriched with Mn(II) resulted in the elevation of the manganese-containing superoxide dismutase, whereas growth in such medium enriched with iron caused increased content of the iron-containing superoxide dismutase. Enrichment of the medium with Co(II), Cu(II), Mo(VI), Zn(I...

journal_title：Journal of bacteriology

authors： Pugh SY,DiGuiseppi JL,Fridovich I

更新日期：1984-10-01 00:00:00

abstract：:The regulatory element NosR was identified within the nos region of the denitrification gene cluster of Pseudomonas stutzeri ZoBell (ATCC 14405) and characterized. It is essential for expression of the N2O reductase encoded by nosZ immediately downstream of nosR. The nosR region was initially identified by Tn5 mutagen...

journal_title：Journal of bacteriology

authors： Cuypers H,Viebrock-Sambale A,Zumft WG

更新日期：1992-08-01 00:00:00

abstract：:A phospholipase hydrolyzing cardiolipin to phosphatidic acid and phosphatidyl glycerol was characterized in gram-negative bacteria but was absent in preparations of gram-positive bacteria, Saccharomyces cerevisiae, and rat liver mitochondria. In cell-free extracts of Escherichia coli, Salmonella typhimurium, Proteus v...

journal_title：Journal of bacteriology

authors： Cole R,Proulx P

更新日期：1975-12-01 00:00:00

abstract：:Pseudomonas fluorescens strain KU-7 is a prototype microorganism that metabolizes 2-nitrobenzoate (2-NBA) via the formation of 3-hydroxyanthranilate (3-HAA), a known antioxidant and reductant. The initial two steps leading to the sequential formation of 2-hydroxy/aminobenzoate and 3-HAA are catalyzed by a NADPH-depend...

journal_title：Journal of bacteriology

authors： Iwaki H,Muraki T,Ishihara S,Hasegawa Y,Rankin KN,Sulea T,Boyd J,Lau PC

更新日期：2007-05-01 00:00:00

abstract：:The expression of any given bacterial protein is predicted to depend on (i) the transcriptional regulation of the promoter and the translational regulation of its mRNA and (ii) the synthesis and translation of total (bulk) mRNA. This is because total mRNA acts as a competitor to the specific mRNA for the binding of in...

journal_title：Journal of bacteriology

authors： Liang ST,Xu YC,Dennis P,Bremer H

更新日期：2000-06-01 00:00:00

abstract：:The Zymomonas mobilis gene (sacA) encoding a protein with sucrase activity has been cloned in Escherichia coli and its nucleotide sequence has been determined. Potential ribosome-binding site and promoter sequences were identified in the region upstream of the gene which were homologous to E. coli and Z. mobilis conse...

journal_title：Journal of bacteriology

authors： Gunasekaran P,Karunakaran T,Cami B,Mukundan AG,Preziosi L,Baratti J

更新日期：1990-12-01 00:00:00

abstract：:Strain C5.84 is a Tn5-751 insertion mutant of Aeromonas hydrophila which is unable to secrete extracellular proteins, instead accumulating them in the periplasm (B. Jiang and S.P. Howard, J. Bacteriol. 173:1241-1249, 1991). A 3.5-kb BglII fragment which complements this mutation was isolated from the chromosome of the...

journal_title：Journal of bacteriology

authors： Jahagirdar R,Howard SP

更新日期：1994-11-01 00:00:00

abstract：:Chlorate-resistant mutants corresponding to each known genetic locus (chlA, chlB, chlC, chlD, chlE) were isolated from Escherichia coli K-12. All these mutants showed decreased amounts of membrane-bound nitrate reductase, cytochrome b, and formic dehydrogenase, but all had normal succinic dehydrogenase activity. Prote...

journal_title：Journal of bacteriology

authors： MacGregor CH,Schnaitman CA

更新日期：1971-10-01 00:00:00

abstract：:Pseudomonas sp. VLB120 uses styrene as a sole source of carbon and energy. The first step in this metabolic pathway is catalyzed by an oxygenase (StyA) and a NADH-flavin oxidoreductase (StyB). Both components have been isolated from wild-type Pseudomonas strain VLB120 as well as from recombinant Escherichia coli. StyA...

journal_title：Journal of bacteriology

authors： Otto K,Hofstetter K,Röthlisberger M,Witholt B,Schmid A

更新日期：2004-08-01 00:00:00

abstract：:A novel genetic scheme allowed us to isolate extragenic suppressor mutations that restored mutant OmpF assembly. One group of these mutations, termed asmB for assembly suppressor mutation B, permitted mutant OmpF assembly in a non-allele-specific manner. Genetic mapping analyses placed the asmB mutations at the 2-min ...

journal_title：Journal of bacteriology

authors： Kloser AW,Laird MW,Misra R

更新日期：1996-09-01 00:00:00

abstract：:We have examined the role of the uvrC gene in UV excision repair by studying incision, excision, repair synthesis, and DNA strand reformation in Escherichia coli mutants made permeable to nucleoside triphosphates by toluene treatment. After irradiation, incisions occur normally in uvrC cells in the presence of nicotin...

journal_title：Journal of bacteriology

authors： Sharma S,Moses RE

更新日期：1979-01-01 00:00:00

abstract：:In both prokaryotes and eukaryotes, hydroxyurea is suggested to inhibit DNA replication by inactivating ribonucleotide reductase and depleting deoxyribonucleoside triphosphate pools. In this study, we show that the inhibition of replication in Escherichia coli is transient even at concentrations of 0.1 M hydroxyurea a...

journal_title：Journal of bacteriology

authors： Nazaretyan SA,Savic N,Sadek M,Hackert BJ,Courcelle J,Courcelle CT

更新日期：2018-02-23 00:00:00

abstract：:A key step in the regulation of heat shock genes in Escherichia coli is the stress-dependent degradation of the heat shock promoter-specific sigma(32) subunit of RNA polymerase by the AAA protease, FtsH. Previous studies implicated the C termini of protein substrates, including sigma(32), as degradation signals for AA...

journal_title：Journal of bacteriology

authors： Tomoyasu T,Arsène F,Ogura T,Bukau B

更新日期：2001-10-01 00:00:00

abstract：:By using the isotope pairs (22)Na-(24)Na and (42)K-(86)Rb, the uptake and retention of Na and K was studied in the salt-tolerant Debaryomyces hansenii and in the less tolerant Saccharomyces cerevisiae at NaCl levels of 4 mm and 0.68, 1.35, and 2.7 m in the medium. The ratio of K to Na is much higher in the cells than ...

journal_title：Journal of bacteriology

authors： Norkrans B,Kylin A

更新日期：1969-11-01 00:00:00

abstract：:The R1162-encoded protein RepIB is essential for replication of the plasmid and binds specifically to iterons within the replicative origin. The protein causes the localized melting of DNA (determined by sensitivity to P1 nuclease) at a site within the AT-rich region of the origin, about 60 bp from the iteron binding ...

journal_title：Journal of bacteriology

authors： Kim YJ,Meyer RJ

更新日期：1991-09-01 00:00:00

abstract：:Resting cells of Escherichia coli are able to initiate growth and murein biosynthesis in the presence of beta-lactam antibiotics binding to penicillin-binding proteins (PBPs) 1a and 1b (E. J. de la Rosa, M. A. de Pedro, and D. Vázquez, Proc. Natl. Acad. Sci. USA 82:5632-5635, 1985). Under these conditions, cells elong...

journal_title：Journal of bacteriology

authors： García-del Portillo F,Pisabarro AG,de la Rosa EJ,de Pedro MA

更新日期：1987-06-01 00:00:00

abstract：:Moulder, James W. (University of Chicago, Chicago, Ill.), Dorothy L. Novosel, and Ilse I. E. Tribby. Changes in mouse pneumonitis agent associated with development of resistance to chlortetracycline. J. Bacteriol. 89:17-22. 1965.-A chlortetracycline-resistant mutant of mouse pneumonitis agent, a member of the psittaco...

journal_title：Journal of bacteriology

authors： MOULDER JW,NOVOSEL DL,TRIBBY II

更新日期：1965-01-01 00:00:00

abstract：:A hydrocarbon-utilizing Brevibacterium which grew into the oil phase of an oil-water system was tested for survival at elevated temperature. Cells suspended in oil and cells that had been resuspended in aqueous solution were tested by placing 1-ml samples of the cell suspension in small test tubes immersed in a contro...

journal_title：Journal of bacteriology

authors： Severance MM,LaRock PA

更新日期：1973-12-01 00:00:00

abstract：:The master regulator for entry into sporulation in Bacillus subtilis is the response regulator Spo0A, which directly governs the expression of about 121 genes. Using cells in which the synthesis of Spo0A was under the control of an inducible promoter or in which production of the regulatory protein was impaired by a p...

journal_title：Journal of bacteriology

authors： Fujita M,González-Pastor JE,Losick R

更新日期：2005-02-01 00:00:00

abstract：:The gene encoding an extracellular isomalto-dextranase, designated imd, was isolated from the chromosomal DNA of Arthrobacter globiformis T6 and cloned and expressed in Escherichia coli. A single open reading frame consisting of 1,926 base pairs that encoded a polypeptide composed of a signal peptide of 39 amino acids...

journal_title：Journal of bacteriology

authors： Iwai A,Ito H,Mizuno T,Mori H,Matsui H,Honma M,Okada G,Chiba S

更新日期：1994-12-01 00:00:00

abstract：:Quorum sensing of Sinorhizobium meliloti relies on N-acyl-homoserine lactones (AHLs) as autoinducers. AHL production increases at high population density, and this depends on the AHL synthase SinI and two transcriptional regulators, SinR and ExpR. Our study demonstrates that ectopic expression of the gene rne, coding ...

journal_title：Journal of bacteriology

authors： Baumgardt K,Charoenpanich P,McIntosh M,Schikora A,Stein E,Thalmann S,Kogel KH,Klug G,Becker A,Evguenieva-Hackenberg E

更新日期：2014-04-01 00:00:00

abstract：UNLABELLED:Clostridium perfringens type D strains are usually associated with diseases of livestock, and their virulence requires the production of epsilon toxin (ETX). We previously showed (J. Li, S. Sayeed, S. Robertson, J. Chen, and B. A. McClane, PLoS Pathog 7:e1002429, 2011, http://dx.doi.org/10.1371/journal.ppat....

journal_title：Journal of bacteriology

authors： Li J,Freedman JC,McClane BA

更新日期：2015-10-01 00:00:00