Membrane association of conjugally transferred deoxyribonucleic acid in Escherichia coli minicells.

abstract：:A series of mutations of the P1 plasmid prophage that lead to increased copy number was isolated and analyzed. The copy number of the mutants was elevated at least five- to eightfold relative to wild-type P1, as determined by single-cell resistance to antibiotics, activity of enzymes, content of superhelical DNA, and ...

journal_title：Journal of bacteriology

authors： Scott JR,Kropf MM,Padolsky L,Goodspeed JK,Davis R,Vapnek D

更新日期：1982-06-01 00:00:00

abstract：:Virulence genes in Listeria monocytogenes are coordinately expressed under the control of the transcriptional activator PrfA, encoded by prfA, a member of the cyclic AMP (cAMP) receptor protein (CRP)/FNR family of bacterial regulators. Strain P14-A is a spontaneous mutant of L. monocytogenes serovar 4b which produces ...

journal_title：Journal of bacteriology

authors： Ripio MT,Domínguez-Bernal G,Lara M,Suárez M,Vazquez-Boland JA

更新日期：1997-03-01 00:00:00

abstract：:Daniel, John W. (University of Wisconsin, Madison), and Karlee Babcock. Methionine metabolism of the myxomycete Physarum polycephalum. J. Bacteriol. 92:1028-1035. 1966.-Previous studies have shown that Physarum polycephalum requires exogenous methionine for growth, but not cysteine, folic acid, or vitamin B(12). Methi...

journal_title：Journal of bacteriology

authors： Daniel JW,Babcock K

更新日期：1966-10-01 00:00:00

abstract：:The bacterial peptidoglycan consists of glycan chains of repeating beta-1,4-linked N-acetylglucosaminyl-N-acetylmuramyl units cross-linked through short peptide chains. The polymerization of the glycans, or glycosyltransfer (GT), and transpeptidation (TP) are catalyzed by bifunctional penicillin-binding proteins (PBPs...

journal_title：Journal of bacteriology

authors： Di Guilmi AM,Dessen A,Dideberg O,Vernet T

更新日期：2003-08-01 00:00:00

abstract：:Study of the replication pattern of a number of B. subtilis 168 strains under controlled physiological conditions revealed great interstrain variation in control of replication. Replication patterns were calculated from ratios of purA16/leu-8 and purA16/metB5 transformation frequency. The thymine-independent strains a...

journal_title：Journal of bacteriology

authors： Gillin FD,Ganesan AT

更新日期：1975-09-01 00:00:00

abstract：:The genes coding for two structurally different isocitrate dehydrogenase isozymes (IDH-I and IDH-II) of a psychrophilic bacterium, Vibrio sp. strain ABE-1, were cloned and sequenced. Open reading frames of the genes (icdI and icdII) are 1,248 and 2,229 bp in length, respectively. The amino acid sequences predicted fro...

journal_title：Journal of bacteriology

authors： Ishii A,Suzuki M,Sahara T,Takada Y,Sasaki S,Fukunaga N

更新日期：1993-11-01 00:00:00

abstract：:The upper operon of the TOL plasmid pWW0 of Pseudomonas putida encodes a set of enzymes which transform toluene and xylenes to benzoate and toluates. The genetic organization of the operon was characterized by cloning of the upper operon genes into an expression vector and identification of their products in Escherich...

journal_title：Journal of bacteriology

authors： Harayama S,Rekik M,Wubbolts M,Rose K,Leppik RA,Timmis KN

更新日期：1989-09-01 00:00:00

abstract：:Flagellar motility is an important determinant of Campylobacter jejuni that is required for promoting interactions with various hosts to promote gastroenteritis in humans or commensal colonization of many animals. In a previous study, we identified a nonmotile mutant of C. jejuni 81-176 with a transposon insertion in ...

journal_title：Journal of bacteriology

authors： Sommerlad SM,Hendrixson DR

更新日期：2007-01-01 00:00:00

abstract：:A 7.5-kbp fragment of chromosomal DNA downstream of the Vibrio cholerae vibriobactin outer membrane receptor, viuA, and the vibriobactin utilization gene, viuB, was recovered from a Sau3A lambda library of O395 chromosomal DNA. By analogy with the genetic organization of the Escherichia coli enterobactin gene cluster,...

journal_title：Journal of bacteriology

authors： Butterton JR,Choi MH,Watnick PI,Carroll PA,Calderwood SB

更新日期：2000-03-01 00:00:00

abstract：:Deletions of the Escherichia coli K-12 chromosome between trpR and thr render the bacterium sensitive to the dye toluidine blue (Dye-), and if male (Hfr or F'), the strain is sterile (Fex-), failing to donate F' or chromosomal markers and resistant to male-specific phages as a consequence of its inability to elaborate...

journal_title：Journal of bacteriology

authors： Buxton RS,Drury LS

更新日期：1983-06-01 00:00:00

abstract：:Alpha granules were isolated from a blue-green alga, Nostoc muscorum, in large quantities and high purity by sucrose density gradient centrifugation. Sodium deoxycholate was used to eliminate membrane contamination. Isolated alpha granules from this species have average dimension of 31 nm in width and 65 nm in length....

journal_title：Journal of bacteriology

authors： Chao L,Bowen CC

更新日期：1971-01-01 00:00:00

abstract：:L. Sibold, M. Henriquet, O. Possot, and J.-P. Aubert (Res. Microbiol. 142:5-12, 1991) cloned and sequenced two nifH-homologous open reading frames (ORFs) from Methanosarcina barkeri 227. Phylogenetic analysis of the deduced amino acid sequences of the nifH ORFs from M. barkeri showed that nifH1 clusters with nifH gene...

journal_title：Journal of bacteriology

authors： Chien YT,Zinder SH

更新日期：1994-11-01 00:00:00

abstract：:The host factor (HF-I) for phage Q beta RNA replication is a small protein of 102 amino acid residues encoded by the hfq gene at 94.8 min on the Escherichia coli chromosome. The synthesis rate of HF-I at the exponential-growth phase is higher than at the stationary phase, and it increases concomitantly with the increa...

journal_title：Journal of bacteriology

authors： Kajitani M,Kato A,Wada A,Inokuchi Y,Ishihama A

更新日期：1994-01-01 00:00:00

abstract：:Type 1 pili of Escherichia coli are the prototype of the somatic class of pili found on many strains of bacteria. As a first step in the genetic analysis of type 1 piliation, an extensive series of nonpiliated derivatives of E. coli K-12 strain AW405, was characterized to produce attached or free pili when examined in...

journal_title：Journal of bacteriology

authors： Swaney LM,Liu YP,To CM,To CC,Ippen-Ihler K,Brinton CC Jr

更新日期：1977-04-01 00:00:00

abstract：:Deletions of the uvrB gene were not obtained in polA1 strains of Escherichia coli either by selecting for spontaneous deletions or by transduction from strains carrying such deletions. A strain forming a temperature-sensitive deoxyribonucleic acid polymerase I and carrying a deletion of the uvrB gene is inviable at th...

journal_title：Journal of bacteriology

authors： Shizuya H,Dykhuizen D

更新日期：1972-11-01 00:00:00

abstract：:Ferric reductase activity was examined in Azotobacter vinelandii and was found to be located in the cytoplasm. The specific activities of soluble cell extracts were not affected by the iron concentration of the growth medium; however, activity was inhibited by the presence of Zn2+ during cell growth and also by the ad...

journal_title：Journal of bacteriology

authors： Huyer M,Page WJ

更新日期：1989-07-01 00:00:00

abstract：:A number of independently derived uracil-requiring mutant strains of the parent strain 168 were mapped by inter-mutant transformation crosses. Only a few of these mutant strains were found to be transformable. Studies were performed in which these transformable uracil-requiring mutant strains were used as the recipien...

journal_title：Journal of bacteriology

authors： Kelleher RJ Jr,Gooder H

更新日期：1973-11-01 00:00:00

abstract：:Integrative and conjugative elements (ICEs), also known as conjugative transposons, are mobile genetic elements that can transfer from one bacterial cell to another by conjugation. ICEBs1 is integrated into the trnS-leu2 gene of Bacillus subtilis and is regulated by the SOS response and the RapI-PhrI cell-cell peptide...

journal_title：Journal of bacteriology

authors： Lee CA,Grossman AD

更新日期：2007-10-01 00:00:00

abstract：:A series of intercellular signals are involved in the regulation of gene expression during fruiting body formation of Myxococcus xanthus. Mutations which block cell interactions, such as csgA (formerly known as spoC), also prevent expression of certain developmentally regulated promoters. csgA+ cells containing Tn5 la...

journal_title：Journal of bacteriology

authors： Li SF,Shimkets LJ

更新日期：1988-12-01 00:00:00

abstract：:Clinical "superbug" isolates of Pseudomonas aeruginosa were previously observed to be resistant to several antibiotics, including polymyxin B, and/or to have a distinct, reproducible adaptive polymyxin resistance phenotype, identified by observing "skipped" wells (appearance of extra turbid wells) during broth microdi...

journal_title：Journal of bacteriology

authors： Boyle B,Fernandez L,Laroche J,Kukavica-Ibrulj I,Mendes CM,Hancock RW,Levesque RC

更新日期：2012-01-01 00:00:00

abstract：:Earlier observations that phenol is a repellent for Salmonella typhimurium but an attractant for Escherichia coli were confirmed. This behavioral difference was found to correlate with a difference in the effect phenol had on receptor methylation levels; it caused net demethylation in S. typhimurium but net methylatio...

journal_title：Journal of bacteriology

authors： Imae Y,Oosawa K,Mizuno T,Kihara M,Macnab RM

更新日期：1987-01-01 00:00:00

abstract：:Cosmid B577, a member of the collection of ordered clones corresponding to the genome of Mycobacterium leprae, contains a gene, provisionally called pon1, that encodes an 821-amino-acid-residue high-molecular-mass class A penicillin-binding protein, provisionally called PBP1. With similar amino acid sequences and modu...

journal_title：Journal of bacteriology

authors： Basu J,Mahapatra S,Kundu M,Mukhopadhyay S,Nguyen-Distèche M,Dubois P,Joris B,Van Beeumen J,Cole ST,Chakrabarti P,Ghuysen JM

更新日期：1996-03-01 00:00:00

abstract：:Environmental iron concentrations coordinately regulate transcription of genes involved in iron acquisition and virulence via the ferric uptake regulation (fur) system. We identified and sequenced the fur gene and flanking regions of three Bartonella species. The most notable difference between Bartonella Fur and othe...

journal_title：Journal of bacteriology

authors： Park SY,Kelminson KL,Lee AK,Zhang P,Warner RE,Rehkopf DH,Calderwood SB,Koehler JE

更新日期：2001-10-01 00:00:00

abstract：:The hpaB gene encoding an aromatic hydroxylase of Escherichia coli ATCC 11105, a penicillin G acylase-producing strain, has been cloned and expressed in E. coli K-12. This gene was located near the pacA gene coding for penicillin G acylase. The hydroxylase has a molecular mass of 59,000 Da, uses NADH as a cosubstrate,...

journal_title：Journal of bacteriology

authors： Prieto MA,Perez-Aranda A,Garcia JL

更新日期：1993-04-01 00:00:00

abstract：:A 40-kb region of DNA from Sorangium cellulosum So ce26, which contains polyketide synthase (PKS) genes for synthesis of the antifungal macrolide antibiotic soraphen A, was cloned. These genes were detected by homology to Streptomyces violaceoruber genes encoding components of granaticin PKS, thus extending this power...

journal_title：Journal of bacteriology

authors： Schupp T,Toupet C,Cluzel B,Neff S,Hill S,Beck JJ,Ligon JM

更新日期：1995-07-01 00:00:00

abstract：:The transforming activity of fractionated complementary strands of Diplococcus pneumoniae deoxyribonucleic acid (DNA) bands at the position of fully denatured DNA in CsCl at pH 11.0, and is completely (> 99.8%) destroyed by digestion with exonuclease-I. These results prove that pure single strands transform the normal...

journal_title：Journal of bacteriology

authors： Miao R,Guild WR

更新日期：1970-02-01 00:00:00

abstract：:Studies were made on the physical and chemical properties of polysaccharides synthesized by cell-free extracts of Streptococcus mutans, Streptococcus sanguis, and Streptococcus sp. and their susceptibilities to dextranases. Among the polysaccharides examined, insoluble glucans were rather resistant to available dextra...

journal_title：Journal of bacteriology

authors： Ebisu S,Kato K,Kotani S,Misaki A

更新日期：1975-12-01 00:00:00

abstract：:The bacteriophage P22-based challenge-phage system was used to study the binding of Xis and FIS to their sites in attP of bacteriophage lambda. Challenge phages were constructed that contained the X1, X2, and F sites within the P22 Pant promoter, which is required for expression of antirepressor. If Xis and FIS bind t...

journal_title：Journal of bacteriology

authors： Numrych TE,Gumport RI,Gardner JF

更新日期：1991-10-01 00:00:00

abstract：:Mutants with reduced lysine decarboxylase activity were isolated from an Escherichia coli polyamine auxotroph. These mutants could not produce induced enzyme, showing only a very low level of an apparently constitutive form. Both enzyme forms could be demonstrated in the parental strain. ...

journal_title：Journal of bacteriology

authors： Goldemberg SH

更新日期：1980-03-01 00:00:00

abstract：:Previous microarray studies have suggested that an indirect mechanism of Fur regulation may be present in meningococcus at the posttranscriptional level through a small regulatory RNA (sRNA) system analogous to that of Escherichia coli and Pseudomonas aeruginosa. Recently, a Fur-regulated sRNA, NrrF, was identified th...

journal_title：Journal of bacteriology

authors： Metruccio MM,Fantappiè L,Serruto D,Muzzi A,Roncarati D,Donati C,Scarlato V,Delany I

更新日期：2009-02-01 00:00:00