Analysis of the roles of FlgP and FlgQ in flagellar motility of Campylobacter jejuni.

abstract：:SpoVM is an unusually small (26-residue-long) protein that is produced in the mother cell chamber of the sporangium during the process of sporulation in Bacillus subtilis. We investigated the subcellular localization of SpoVM, which is believed to be an amphipathic alpha-helix, by using a fusion of the sporulation pro...

journal_title：Journal of bacteriology

authors： van Ooij C,Losick R

更新日期：2003-02-01 00:00:00

abstract：:We report the use of a fusion to the green fluorescent protein to visualize the assembly of the morphogenetic protein SpoIVA around the developing forespore during the process of sporulation in the bacterium Bacillus subtilis. Using a deconvolution algorithm to process digitally-collected optical sections, we show tha...

journal_title：Journal of bacteriology

authors： Price KD,Losick R

更新日期：1999-02-01 00:00:00

abstract：:The origin of replication of the plasmid R1162 contains an initiation site for the synthesis of each DNA strand. When one of these sites (oriL) is deleted, synthesis on the corresponding strand is no longer initiated efficiently in vitro by the R1162-encoded replication proteins, and the plasmid is no longer stably ma...

journal_title：Journal of bacteriology

authors： Becker EC,Zhou H,Meyer RJ

更新日期：1996-08-01 00:00:00

abstract：:Isolation of six strains of Escherichia coli K-12 carrying mutations affecting the citrate-dependent iron uptake system is described. Genetic analysis of these mutants showed that the mutation affecting the citrate system are cluster together at about min 6 on the E. coli chromosome. ...

journal_title：Journal of bacteriology

authors： Woodrow GC,Langman L,Young IG,Gibson F

更新日期：1978-03-01 00:00:00

abstract：:Amber mutants of bacteriophage P1 were used to identify functions involved in late regulation of the P1 lytic growth cycle. A single function has been genetically identified to be involved in activation of the phage-specific late promoter sequence Ps. In vivo, P1 gene 10 amber mutants fail to trans activate a lacZ ope...

journal_title：Journal of bacteriology

authors： Lehnherr H,Guidolin A,Arber W

更新日期：1991-10-01 00:00:00

abstract：:Undomesticated strains of Bacillus subtilis exhibit extensive colony spreading on certain soft agarose media: first the formation of dendritic clusters of cells, followed by spreading (pellicle-like) growth to cover the entire surface. These phases of colonization are dependent on the level of potassium ion (K(+)) but...

journal_title：Journal of bacteriology

authors： Kinsinger RF,Kearns DB,Hale M,Fall R

更新日期：2005-12-01 00:00:00

abstract：:A series of mutations causing alterations in ribosomal protein S21 was mapped close to dnaG, around 66.5 min on the Escherichia coli chromosomal linkage map. They cotransduced about 95% with this marker. These mutations define gene rpsU, which is very likely the structural gene for protein S21. ...

journal_title：Journal of bacteriology

authors： Dabbs ER

更新日期：1980-11-01 00:00:00

abstract：:A specialized transducing phage lambda carrying the structural gene for the OmpF protein, an outer membrane matrix protein, was isolated. The phage carries the 20.5--21-min region of the Escherichia coli K-12 chromosome and carries asnS, ompF, and aspC genes. ...

journal_title：Journal of bacteriology

authors： Mutoh N,Nagasawa T,Mizushima S

更新日期：1981-02-01 00:00:00

abstract：:The Escherichia coli mutant Y16, which shows thermosensitive colony formation and filamentation with reduced amounts of penicillin-binding protein 3 (PBP3), has mutations in the ftsI gene encoding PBP3 and in the ftsH gene. The ftsI mutation markedly reduces the amount of PBP3 at 42 degrees C, whereas the amount of th...

journal_title：Journal of bacteriology

authors： Begg KJ,Tomoyasu T,Donachie WD,Khattar M,Niki H,Yamanaka K,Hiraga S,Ogura T

更新日期：1992-04-01 00:00:00

abstract：:The last step in proline biosynthesis in Escherichia coli K-12, Salmonella typhimurium LT7, and a number of other enterobacterial isolates is regulated so that no proline is excreted, even if excess Delta(1)-pyrroline-5-carboxylate, the immediate precursor of proline, is added to a culture. In proline auxotrophs block...

journal_title：Journal of bacteriology

authors： Berg CM,Rossi JJ

更新日期：1974-06-01 00:00:00

abstract：:Ruminococcin A (RumA) is a trypsin-dependent lantibiotic produced by Ruminococcus gnavus E1, a gram-positive strict anaerobic strain isolated from a human intestinal microbiota. A 12.8-kb region from R. gnavus E1 chromosome, containing the biosynthetic gene cluster of RumA, has been cloned and sequenced. It consisted ...

journal_title：Journal of bacteriology

authors： Gomez A,Ladiré M,Marcille F,Fons M

更新日期：2002-01-01 00:00:00

abstract：:Pulse-labeled ribonucleic acid (RNA) was extracted from polysomes of sporulating cells of Saccharomyces cerevisiae and characterized in sucrose gradients and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Transfer RNA, ribosomal RNA, and heterodisperse RNA, presumed to be messenger RNA, were synthesized...

journal_title：Journal of bacteriology

authors： Curiale MS,Petryna MM,Mills D

更新日期：1976-05-01 00:00:00

abstract：:Changes in the patterns produced by annealing restriction endonuclease digests of bacterial genomes with probe deoxyribonucleic acids (DNAs) containing small portions of a bacterial genome provide sensitive indicator of the degree of nucleotide sequence relatedness that exists in localized regions of the genomes of cl...

journal_title：Journal of bacteriology

authors： Anilionis A,Riley M

更新日期：1980-07-01 00:00:00

abstract：:Two types of mutants unable to excrete hemolysin were obtained when E. coli 5K carrying the multicopy hemolytic recombinant plasmid pANN202-312 was mutagenized with Mu d1. One type is altered in the plasmid hly-specific gene, hlyBb, but the other is caused by an insertion of Mu d1 into a chromosomal locus. ...

journal_title：Journal of bacteriology

authors： Juarez A,Goebel W

更新日期：1984-09-01 00:00:00

abstract：:An aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646 was purified 196-fold by a combination of Mono-Q, Reactive Green 19 agarose affinity, and hydroxyapatite chromatographies. The purified enzyme runs as a single band of 140 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular ...

journal_title：Journal of bacteriology

authors： Li T,Rosazza JP

更新日期：1997-06-01 00:00:00

abstract：:Estrada-Parra, Sergio (Escuela Nacional de Ciencias Biológicas, México, D.F., México), Abel Zamora, and L. F. Bojalil. Immunochemistry of the group-specific polysaccharide of Nocardia brasiliensis. J. Bacteriol. 90:571-574. 1965.-The group-specific polysaccharide of Nocardia brasiliensis was further purified, yielding...

journal_title：Journal of bacteriology

authors： Estrada-Parra S,Zamora A,Bojalil LF

更新日期：1965-09-01 00:00:00

abstract：:The recF gene of Escherichia coli is known to encode an Mr-40,000 protein that is involved in DNA recombinationa nd postreplication DNA repair. To characterize the role of the recF gene product in these processes, the recF gene was cloned downstream of a tac promoter to facilitate overproduction of the recF gene produ...

journal_title：Journal of bacteriology

authors： Griffin TJ 4th,Kolodner RD

更新日期：1990-11-01 00:00:00

abstract：:The DNA region encoding early nodulation functions of Bradyrhizobium japonicum 3I1b110 (I110) was isolated by its homology to the functionally similar region from Rhizobium meliloti. Isolation of a number of overlapping recombinant clones from this region allowed the construction of a restriction map of the region. Th...

journal_title：Journal of bacteriology

authors： Russell P,Schell MG,Nelson KK,Halverson LJ,Sirotkin KM,Stacey G

更新日期：1985-12-01 00:00:00

abstract：:Five Spirochaeta aurantia chemotaxis mutants were isolated. One mutant (the che-101 mutant) never reversed, one (the che-200 mutant) flexed predominantly, two (the che-300 and che-400-1 mutants) exhibited elevated reversal frequencies, and one (the che-400 mutant) exhibited chemotactically unstimulated behavior simila...

journal_title：Journal of bacteriology

authors： Fosnaugh K,Greenberg EP

更新日期：1989-01-01 00:00:00

abstract：:Hook-associated proteins (HAPs) were excreted into the culture medium of the Fla+ strain as well as into the growth medium of the filamentless mutants of Salmonella typhimurium. This indicates that the bacteria synthesize HAPs excessively, beyond the amount required for construction of flagella. The extra HAPs are she...

journal_title：Journal of bacteriology

authors： Homma M,Iino T

更新日期：1985-12-01 00:00:00

abstract：:A sequenced collection of plasmid-borne random fusions of Escherichia coli DNA to a Photorhabdus luminescens luxCDABE reporter was used as a starting point to select a set of 689 nonredundant functional gene fusions. This group, called LuxArray 1.0, represented 27% of the predicted transcriptional units in E. coli. Hi...

journal_title：Journal of bacteriology

authors： Van Dyk TK,DeRose EJ,Gonye GE

更新日期：2001-10-01 00:00:00

abstract：:The TcbR transcriptional activator protein, which is encoded by the tcbR gene of Pseudomonas sp. strain P51 (J. R. van der Meer, A. C. J. Frijters, J. H. J. Leveau, R. I. L. Eggen, A. J. B. Zehnder, and W. M. de Vos, J. Bacteriol. 173:3700-3708, 1991), was purified from overproducing Escherichia coli cells by using a ...

journal_title：Journal of bacteriology

authors： Leveau JH,de Vos WM,van der Meer JR

更新日期：1994-04-01 00:00:00

abstract：:A strain of Escherichia coli with a mutation in the promoter proximal gene ( uncI ) of the unc operon has been constructed by using a new gene replacement method. The mutation is a deletion of a defined sequence of 196 base pairs. It was constructed by homologous integration and segregation of a ColE1-derived recombin...

journal_title：Journal of bacteriology

authors： Gay NJ

更新日期：1984-06-01 00:00:00

abstract：:The effect of adding hemin to anaerobically grown cells of a strain of Staphylococcus epidermidis, which was heme-deficient due to anaerobic growth, has been examined. Cells grown anaerobically in media containing hemin exhibited a marked increase in several oxidative activities as compared with cells grown anaerobica...

journal_title：Journal of bacteriology

authors： Jacobs NJ,Maclosky ER,Conti SF

更新日期：1967-01-01 00:00:00

abstract：:The Escherichia coli htrD gene was originally isolated during a search for new genes required for growth at high temperature. Insertional inactivation of htrD leads to a pleiotropic phenotype characterized by temperature-sensitive growth in rich medium, H2O2 sensitivity, and sensitivity to cysteine. The htrD gene was ...

journal_title：Journal of bacteriology

authors： Delaney JM,Wall D,Georgopoulos C

更新日期：1993-01-01 00:00:00

abstract：:Specific changes in the chemical and microbial composition of Thermoanaerobium brockii fermentations were compared and related to alterations of process rates, end product yields, and growth parameters. Fermentation of starch as compared with glucose was associated with significant decreases in growth rate and intrace...

journal_title：Journal of bacteriology

authors： Ben-Bassat A,Lamed R,Zeikus JG

更新日期：1981-04-01 00:00:00

abstract：:Genetic transformation of Sulfolobus acidocaldarius by a multiply marked pyrE gene provided a high-resolution assay of homologous recombination in a hyperthermophilic archaeon. Analysis of 100 Pyr(+) transformants revealed that this recombination system could transfer each of 23 nonselected base pair substitutions to ...

journal_title：Journal of bacteriology

authors： Grogan DW,Rockwood J

更新日期：2010-09-01 00:00:00

abstract：:An m(k) (-)r(k) (-) mutation exerted no measurable effect on 5-bromouracil incorporation by a thymineless derivative of Escherichia coli K. ...

journal_title：Journal of bacteriology

authors： Matney TS,MacDougall NL,Butler MA,Suit JC

更新日期：1970-10-01 00:00:00

abstract：:Methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila TM-1 was purified 16-fold from a cell extract to apparent homogeneity as determined by native polyacrylamide gel electrophoresis. Ninety-four percent of the methylreductase activity was recovered in the soluble fraction of cell extracts. T...

journal_title：Journal of bacteriology

authors： Jablonski PE,Ferry JG

更新日期：1991-04-01 00:00:00

abstract：:Sequence analysis of the large virulence plasmid pLVPK in Klebsiella pneumoniae CG43 revealed the presence of another mucoid factor encoding gene rmpA besides rmpA2. Promoter activity measurement indicated that the deletion of rmpA reduced K2 capsular polysaccharide (CPS) biosynthesis, resulting in decreased colony mu...

journal_title：Journal of bacteriology

authors： Cheng HY,Chen YS,Wu CY,Chang HY,Lai YC,Peng HL

更新日期：2010-06-01 00:00:00