Abstract:
:A 7.5-kbp fragment of chromosomal DNA downstream of the Vibrio cholerae vibriobactin outer membrane receptor, viuA, and the vibriobactin utilization gene, viuB, was recovered from a Sau3A lambda library of O395 chromosomal DNA. By analogy with the genetic organization of the Escherichia coli enterobactin gene cluster, in which the enterobactin biosynthetic and transport genes lie adjacent to the enterobactin outer membrane receptor, fepA, and the utilization gene, fes, the cloned DNA was examined for the ability to restore siderophore synthesis to E. coli ent mutants. Cross-feeding studies demonstrated that an E. coli entF mutant complemented with the cloned DNA regained the ability to synthesize enterobactin and to grow in low-iron medium. Sequence analysis of the cloned chromosomal DNA revealed an open reading frame downstream of viuB which encoded a deduced protein of greater than 2,158 amino acids, homologous to Yersinia sp. HMWP2, Vibrio anguillarum AngR, and E. coli EntF. A mutant with an in-frame deletion of this gene, named vibF, was created with classical V. cholerae strain O395 by in vivo marker exchange. In cross-feeding studies, this mutant was unable to synthesize ferric vibriobactin but was able to utilize exogenous siderophore. Complementation of the mutant with a cloned vibF fragment restored vibriobactin synthesis to normal. The expression of the vibF promoter was found to be negatively regulated by iron at the transcriptional level, under the control of the V. cholerae fur gene. Expression of vibF was not autoregulatory and neither affected nor was affected by the expression of irgA or viuA. The promoter of vibF was located by primer extension and was found to contain a dyad symmetric nucleotide sequence highly homologous to the E. coli Fur binding consensus sequence. A footprint of purified V. cholerae Fur on the vibF promoter, overlapping the Fur binding consensus sequence, was observed using DNase I footprinting. The protein product of vibF is homologous to the multifunctional nonribosomal protein synthetases and is necessary for the biosynthesis of vibriobactin.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Butterton JR,Choi MH,Watnick PI,Carroll PA,Calderwood SBdoi
10.1128/jb.182.6.1731-1738.2000keywords:
subject
Has Abstractpub_date
2000-03-01 00:00:00pages
1731-8issue
6eissn
0021-9193issn
1098-5530journal_volume
182pub_type
杂志文章abstract::Knaysi, Georges (Cornell University, Ithaca, N.Y.). Effect of temperature on the rate of germination in Bacillus cereus. J. Bacteriol. 87:619-622. 1964.-By use of the change of the spores from bright to dark, when observed in dark contrast with a phase microscope, as a criterion for the incipience (first stage) of ger...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.3.619-622.1964
更新日期:1964-03-01 00:00:00
abstract::Three homofermentative (Lactobacillus plantarum B38, L. plantarum B33, Pediococcus pentosaceus B30) and three heterofermentative (Leuconostoc mesenteroides 39, L. oenos B70, Lactobacillus brevis) lactic acid bacteria were examined for the presence or absence of nicotinamide adenine dinucleotide (NAD)-dependent and NAD...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.3.1284-1289.1971
更新日期:1971-12-01 00:00:00
abstract::Micromonospora strains have been isolated from diverse niches, including soil, water, and marine sediments and root nodules of diverse symbiotic plants. In this work, we report the genome sequence of Micromonospora lupini Lupac 08 isolated from root nodules of the wild legume Lupinus angustifolious. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00628-12
更新日期:2012-08-01 00:00:00
abstract::The chb operon of Escherichia coli is involved in the utilization of the β-glucosides chitobiose and cellobiose. The function of chbG (ydjC), the sixth open reading frame of the operon that codes for an evolutionarily conserved protein is unknown. We show that chbG encodes a monodeacetylase that is essential for growt...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00533-12
更新日期:2012-09-01 00:00:00
abstract::Polyamines are required for rapid phospholipid turnover in Escherichia coli growing in media of low osmolarity. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.3.1479-1481.1973
更新日期:1973-12-01 00:00:00
abstract::A new method has been developed in order to select mutants showing decreased puc operon transcription in Rhodobacter sphaeroides 2.4.1. A transcriptional fusion of a promoterless fragment derived from the sacB gene, encoding the levansucrase from Bacillus subtilis, to the upstream regulatory region of the puc operon h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.1.35-45.1996
更新日期:1996-01-01 00:00:00
abstract::DnaA is an AAA+ ATPase and the conserved replication initiator in bacteria. Bacteria control the timing of replication initiation by regulating the activity of DnaA. DnaA binds to multiple sites in the origin of replication (oriC) and is required for recruitment of proteins needed to load the replicative helicase. Dna...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00958-12
更新日期:2012-09-01 00:00:00
abstract::The cytoplasmic accumulation of exogenous betaine stimulates the growth of Lactococcus lactis cultivated under hyperosmotic conditions. We report that L. lactis possesses a single betaine transport system that belongs to the ATP-binding cassette (ABC) superfamily of transporters. Through transposon mutagenesis, a muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.20.6238-6246.1999
更新日期:1999-10-01 00:00:00
abstract::The penultimate step in the biosynthesis of riboflavin (vitamin B2) involves the condensation of 3,4-dihydroxy-2-butanone 4-phosphate with 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, which is catalyzed by 6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase). Pathogenic Brucella species adapted to an intr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00207-06
更新日期:2006-09-01 00:00:00
abstract::Bacteriophage GA-1 infects Bacillus sp. strain G1R and has a linear double-stranded DNA genome with a terminal protein covalently linked to its 5' ends. GA-1 protein p6 is very abundant in infected cells and binds DNA with no sequence specificity. We show here that it binds in vivo to the whole viral genome, as detect...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01047-07
更新日期:2007-11-01 00:00:00
abstract::The Gifsy-1 phage integrates site specifically into the Salmonella chromosome via an integrase-mediated site-specific recombination mechanism. Initial genetic analysis suggests that Gifsy-1 integrase-mediated excision of the Gifsy-1 phage is influenced by proteins encoded by both the Gifsy-1 and the Gifsy-2 phages. Ou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00577-07
更新日期:2007-09-01 00:00:00
abstract::Addition of purines to the growth medium of Escherichia coli represses synthesis of cytosine deaminase (codA) and enzymes of purine de novo synthesis. After Tn10 mutagenesis, mutants displaying derepressed levels of cytosine deaminase in the presence of hypoxanthine were isolated. One of these had simultaneously acqui...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.2124-2127.1989
更新日期:1989-04-01 00:00:00
abstract::SecA signal peptide interaction is critical for initiating protein translocation in the bacterial Sec-dependent pathway. Here, we have utilized the recent nuclear magnetic resonance (NMR) and Förster resonance energy transfer studies that mapped the location of the SecA signal peptide-binding site to design and isolat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06150-11
更新日期:2012-01-01 00:00:00
abstract::A temperature-sensitive Schizosaccharomyces pombe mutant, cdc16-116, has been isolated which undergoes uncontrolled septation during its cell division cycle. The mutant accumulates two types of cells after 3 h of growth at the restrictive temperature: (i) type I cells (85% of the population), which complete nuclear di...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.1.440-446.1979
更新日期:1979-01-01 00:00:00
abstract::The heptapeptide-nucleotide microcin C (McC) targets aspartyl-tRNA synthetase. Upon its entry into a susceptible cell, McC is processed to release a nonhydrolyzable aspartyl-adenylate that inhibits aspartyl-tRNA synthetase, leading to the cessation of translation and cell growth. Here, we surveyed Escherichia coli cel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01956-07
更新日期:2008-04-01 00:00:00
abstract:UNLABELLED:Campylobacter jejuni is a leading cause of bacterial diarrheal disease and a frequent commensal of the intestinal tract in poultry and other animals. For optimal growth and colonization of hosts, C. jejuni employs two-component regulatory systems (TCSs) to monitor environmental conditions and promote proper ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02564-14
更新日期:2015-05-01 00:00:00
abstract::The effect of the iron content of the medium on the yields of extracellular products by seven distinct strains of Pseudomonas aeruginosa was examined. All strains showed at least an 85% decrease in toxin A yields when grown in medium containing 5.0 mug of iron per ml (high iron) as compared to 0.05 mug/ml (low iron), ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.1.193-200.1979
更新日期:1979-04-01 00:00:00
abstract::Expression of the Escherichia coli OmpC and OmpF outer membrane proteins is regulated by the osmolarity of the culture media. In contrast, expression of OmpC in Salmonella typhi is not influenced by osmolarity, while OmpF is regulated as in E. coli. To better understand the lack of osmoregulation of OmpC expression in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.2.556-562.1999
更新日期:1999-01-01 00:00:00
abstract::The Azorhizobium nifA promoter (PnifA) is positively regulated by two physiological signal transduction pathways, NtrBC, which signals anabolic N status, and FixLJK, which signals prevailing O2 status. Yet, PnifA response (gene product per unit time) to these two activating signals together is more than twice that of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.24.7210-7221.1995
更新日期:1995-12-01 00:00:00
abstract:UNLABELLED:FimV is a Pseudomonas aeruginosa inner membrane protein that regulates intracellular cyclic AMP (cAMP) levels-and thus type IV pilus (T4P)-mediated twitching motility and type II secretion (T2S)-by activating the adenylate cyclase CyaB. Its cytoplasmic domain contains three predicted tetratricopeptide repeat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00322-16
更新日期:2016-07-28 00:00:00
abstract::Mutants of Escherichia coli capable of growing in the presence of 10 microgram of mecillinam per ml were selected after intensive mutagenesis. Of these mutants, 1.4% formed normal, rod-shaped cells at 30 degrees C but grew as spherical cells at 42 degrees C. The phenotype of one of these rod(Ts) mutants was 88% cotran...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.3.1143-1158.1978
更新日期:1978-12-01 00:00:00
abstract::Hook-associated proteins (HAPs) were excreted into the culture medium of the Fla+ strain as well as into the growth medium of the filamentless mutants of Salmonella typhimurium. This indicates that the bacteria synthesize HAPs excessively, beyond the amount required for construction of flagella. The extra HAPs are she...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.3.1370-1372.1985
更新日期:1985-12-01 00:00:00
abstract::The cheA locus of Escherichia coli encodes two similar proteins, CheAL (654 amino acids) and CheAS (557 amino acids), which are made by initiating translation from different in-frame start sites [start(L) and start(S)]. CheAL plays an essential role in chemotactic signaling. It autophosphorylates at a histidine residu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.10.2713-2720.1995
更新日期:1995-05-01 00:00:00
abstract::Massetolide A is a cyclic lipopeptide (CLP) antibiotic produced by various Pseudomonas strains from diverse environments. Cloning, sequencing, site-directed mutagenesis, and complementation showed that massetolide A biosynthesis in P. fluorescens SS101 is governed by three nonribosomal peptide synthetase (NRPS) genes,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01563-07
更新日期:2008-04-01 00:00:00
abstract::t is the holin gene for coliphage T4, encoding a 218-amino-acid (aa) protein essential for the inner membrane hole formation that initiates lysis and terminates the phage infection cycle. T is predicted to be an integral membrane protein that adopts an N(in)-C(out) topology with a single transmembrane domain (TMD). Th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01548-14
更新日期:2014-06-01 00:00:00
abstract::Vibrio vulnificus is a causative agent of serious food-borne diseases in humans related to the consumption of raw seafood. It secretes a metalloprotease that is associated with skin lesions and serious hemorrhagic complications. In this study, we purified and characterized an extracellular metalloprotease (designated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.20.6909-6916.2005
更新日期:2005-10-01 00:00:00
abstract::Seligman, Stephen J. (University of California, Los Angeles) and William L. Hewitt. Kinetics of the action of ampicillin on Escherichia coli. J. Bacteriol. 85:1160-1164. 1963.-The curve of the number of viable Escherichia coli after exposure to ampicillin can be divided into three phases: a lag phase, a rapid bacteric...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.5.1160-1164.1963
更新日期:1963-05-01 00:00:00
abstract::We previously developed a genetic approach to study, with a single antibody, the topology of the outer membrane protein LamB, an Escherichia coli porin with specificity towards maltodextrins and a receptor for bacteriophage lambda. Our initial procedure consisted of inserting at random the same reporter epitope (the C...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.12.3447-3456.1996
更新日期:1996-06-01 00:00:00
abstract::In previous work, we characterized the bases in an iteron of plasmid R6K that are important for the binding of pi protein monomers and dimers. Here we investigate the following six amino acids of pi, encoded by pir, hypothesized to be important for DNA contact: Ser71, Try74, Gly131, Gly211, Arg225, and Arg254. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00109-07
更新日期:2007-07-01 00:00:00
abstract::Membrane vesicles isolated from competent cultures of Bacillus subtilis 168 bound up to 20 mug of double-stranded deoxyribonucleic acid (DNA) per mg of membrane protein in the presence of ethylenediaminetetraacetate. The formation of the DNA-membrane complex was time, temperature, and pH dependent. Eighty per cent of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.3.784-794.1974
更新日期:1974-09-01 00:00:00