Isolation and purification of Flavobacterium alpha-1,3-glucanase-hydrolyzing, insoluble, sticky glucan of Streptococcus mutans.

abstract：:The pdxK and pdxY genes have been found to code for pyridoxal kinases, enzymes involved in the pyridoxal phosphate salvage pathway. Two pyridoxal kinase structures have recently been published, including Escherichia coli pyridoxal kinase 2 (ePL kinase 2) and sheep pyridoxal kinase, products of the pdxY and pdxK genes,...

journal_title：Journal of bacteriology

authors： Safo MK,Musayev FN,di Salvo ML,Hunt S,Claude JB,Schirch V

更新日期：2006-06-01 00:00:00

abstract：:Heme A, as a prosthetic group, is found exclusively in respiratory oxidases of mitochondria and aerobic bacteria. Bacillus subtilis CtaA and other heme A synthases catalyze the conversion of a methyl side group on heme O into a formyl group. The catalytic mechanism of heme A synthase is not understood, and little is k...

journal_title：Journal of bacteriology

authors： Hederstedt L,Lewin A,Throne-Holst M

更新日期：2005-12-01 00:00:00

abstract：:Iron-sulfur flavoproteins (ISF) constitute a widespread family of redox-active proteins in anaerobic prokaryotes. Based on sequence homologies, their overall structure is expected to be similar to that of flavodoxins, but in addition to a flavin mononucleotide cofactor they also contain a cubane-type [4Fe:4S] cluster....

journal_title：Journal of bacteriology

authors： Andrade SL,Cruz F,Drennan CL,Ramakrishnan V,Rees DC,Ferry JG,Einsle O

更新日期：2005-06-01 00:00:00

abstract：:The effects of exogenous reducing agents on a number of biological properties of purified Chlamydia trachomatis LGV-434 and Chlamydia psittaci meningopneumonitis elementary bodies (EBs) have been examined in an attempt to identify in vitro correlates of early events in the differentiation of the infectious EB to the r...

journal_title：Journal of bacteriology

authors： Hackstadt T,Todd WJ,Caldwell HD

更新日期：1985-01-01 00:00:00

abstract：:The iron-molybdenum cofactor (FeMo-co) of nitrogenase contains molybdenum, iron, sulfur, and homocitrate in a ratio of 1:7:9:1. In vitro synthesis of FeMo-co has been established, and the reaction requires an ATP-regenerating system, dithionite, molybdate, homocitrate, and at least NifB-co (the metabolic product of Ni...

journal_title：Journal of bacteriology

authors： Shah VK,Rangaraj P,Chatterjee R,Allen RM,Roll JT,Roberts GP,Ludden PW

更新日期：1999-05-01 00:00:00

abstract：:Shah, D. B. (University of Wisconsin, Madison), and J. B. Wilson. Egg yolk factor of Staphylococcus aureus. II. Characterization of the lipase activity. J. Bacteriol. 89:949-953. 1965.-The staphylococcal egg yolk factor was characterized as a lipase. The enzyme had an optimal pH of 7.8, but the optimal pH of stability...

journal_title：Journal of bacteriology

authors： SHAH DB,WILSON JB

更新日期：1965-04-01 00:00:00

abstract：:The gene coding for the Escherichia coli enzyme 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase has been cloned and sequenced. This gene, designated folK, codes for a protein of 159 amino acids, including an amino-terminal methionine. The protein was overexpressed in E. coli MC4100 by cloning the gene behind the l...

journal_title：Journal of bacteriology

authors： Talarico TL,Ray PH,Dev IK,Merrill BM,Dallas WS

更新日期：1992-09-01 00:00:00

abstract：:The effect of L-methionine-DL-sulfoximine, an inhibitor of glutamine synthetase, on the formation of nitrate reductase in the wild-type strain of Neurospora in the presence of ammonium ions and of glutamine was studied. Under conditions in which glutamine synthetase was inactivated, it was found that only glutamine co...

journal_title：Journal of bacteriology

authors： Premakumar R,Sorger GJ,Gooden D

更新日期：1980-07-01 00:00:00

abstract：:We report the use of a fusion to the green fluorescent protein to visualize the assembly of the morphogenetic protein SpoIVA around the developing forespore during the process of sporulation in the bacterium Bacillus subtilis. Using a deconvolution algorithm to process digitally-collected optical sections, we show tha...

journal_title：Journal of bacteriology

authors： Price KD,Losick R

更新日期：1999-02-01 00:00:00

abstract：:Forty-nine human and environmental isolates of Mycobacterium intracellulare and Mycobacterium scrofulaceum were tested for their ability to grow on uric acid and a number of its degradation products. Nearly all (88 to 90%) strains used uric acid or allantoin as a sole nitrogen source; fewer (47 to 69%) used allantoate...

journal_title：Journal of bacteriology

authors： Falkinham JO 3rd,George KL,Parker BC,Gruft H

更新日期：1983-07-01 00:00:00

abstract：:The cyclic dinucleotides cyclic 3′,5′-diguanylate (c-di-GMP) and cyclic 3′,5′-diadenylate (c-di-AMP) have emerged as key components of bacterial signal transduction networks. These closely related second messengers follow the classical general principles of nucleotide signaling by integrating diverse signals into regu...

journal_title：Journal of bacteriology

authors： Tschowri N

更新日期：2016-01-01 00:00:00

abstract：:Rotation of the bacterial flagellar motor is powered by a transmembrane gradient of protons or, in some species, sodium ions. The molecular mechanism of coupling between ion flow and motor rotation is not understood. The proteins most closely involved in motor rotation are MotA, MotB, and FliG. MotA and MotB are trans...

journal_title：Journal of bacteriology

authors： Zhou J,Sharp LL,Tang HL,Lloyd SA,Billings S,Braun TF,Blair DF

更新日期：1998-05-01 00:00:00

abstract：:Evidence is presented that progametangia in both the plus and the minus mating types of Mucor mucedo can be induced by one substance, namely (-)-trisporic acid B. A method is described for the determination of the concentration of the sex factors (trisporone, trisporic acid B, trisporic acid C) in mated cultures of Mu...

journal_title：Journal of bacteriology

authors： Van den Ende H,Wiechmann AH,Reyngoud DJ,Hendriks T

更新日期：1970-02-01 00:00:00

abstract：:Hogg, Robert W. (University of Illinois, Urbana), Chitra S. Biswas, and Harry P. Broquist. Interference with valine and isoleucine biosynthesis by cyclic hydroxamic acids. J. Bacteriol. 90:1265-1270. 1965.-The introduction of a hydroxyl group in a number of common barbiturates, a substitution which converts these comp...

journal_title：Journal of bacteriology

authors： Hogg RW,Biswas CS,Broquist HP

更新日期：1965-11-01 00:00:00

abstract：:The smallest viable cell of Spiroplasma citri is a two-turn helix (elementary helix). This elementary helix grows into longer parental cells, which then divide by constriction. The helical morphology is conserved during this process. The growth pattern of S. citri membranes has been investigated by different methods o...

journal_title：Journal of bacteriology

authors： Garnier M,Clerc M,Bové JM

更新日期：1984-04-01 00:00:00

abstract：:The production of extracellular enzymes such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt) is activated by the cell density (quorum)-sensing signal, N-(3-oxohexanoyl)-L-homoserine lactone (HSL); plant signals; and aep genes during postexponential growth of Erwinia carotovora subs...

journal_title：Journal of bacteriology

authors： Cui Y,Chatterjee A,Liu Y,Dumenyo CK,Chatterjee AK

更新日期：1995-09-01 00:00:00

abstract：:Mutants of Bacillis subtilis that are temperature sensitive for RNA synthesis during sporulation were isolated after selection with a 32P suicide agent. Whole-genome sequencing revealed that two of the mutants carried an identical lesion in the rsbU gene, which encodes a phosphatase that indirectly activates SigB, the...

journal_title：Journal of bacteriology

authors： Rothstein DM,Lazinski D,Osburne MS,Sonenshein AL

更新日期：2017-06-27 00:00:00

abstract：:The nifV and leuA genes, which encode homocitrate synthase and alpha-isopropylmalate synthase, respectively, were cloned from the filamentous cyanobacterium Anabaena sp. strain PCC 7120 by a PCR-based strategy. Since the N-terminal parts of NifV and LeuA from other bacteria are highly similar to each other, a single p...

journal_title：Journal of bacteriology

authors： Stricker O,Masepohl B,Klipp W,Böhme H

更新日期：1997-05-01 00:00:00

abstract：:Ureolytic clinical isolates of Providencia stuartii, Salmonella spp., and some Escherichia coli strains contain large urease-encoding plasmids. Expression of urease activity from these isolates is induced at least 20-fold by urea. In order to facilitate studies on the regulatory mechanism controlling this urea-inducib...

journal_title：Journal of bacteriology

authors： D'Orazio SE,Collins CM

更新日期：1993-06-01 00:00:00

abstract：:A large number of carbon source utilization pathways are repressed in Bacillus subtilis by the global regulator CcpA, which also acts as an activator of carbon excretion pathways during growth in media containing glucose. In this study, CcpA mutants defective in transcriptional activation of the alsSD operon, which is...

journal_title：Journal of bacteriology

authors： Turinsky AJ,Moir-Blais TR,Grundy FJ,Henkin TM

更新日期：2000-10-01 00:00:00

abstract：:In this study, we demonstrated that the surface-expressed enolase from diarrheal isolate SSU of Aeromonas hydrophila bound to human plasminogen and facilitated the latter's tissue-type plasminogen activator-mediated activation to plasmin. The bacterial surface-bound plasmin was more resistant to the action of its spec...

journal_title：Journal of bacteriology

authors： Sha J,Erova TE,Alyea RA,Wang S,Olano JP,Pancholi V,Chopra AK

更新日期：2009-05-01 00:00:00

abstract：:A preparation of pure protoplasts of Geotrichum candidum became osmotically stable and colonies developed when the protoplasts were embedded in stabilizing thin-layer-agar and incubated with stabilizing basal medium. When growing protoplasts were exposed to distilled water and then reincubated with basal medium, the p...

journal_title：Journal of bacteriology

authors： Fukui K,Sagara Y,Yoshida N,Matsuoka T

更新日期：1969-04-01 00:00:00

abstract：:To carry out a genetic analysis of the degradation and utilization of chitin by Serratia marcescens 2170, various Tn5 insertion mutants with characteristic defects in chitinase production were isolated and partially characterized. Prior to the isolation of the mutants, proteins secreted into culture medium in the pres...

journal_title：Journal of bacteriology

authors： Watanabe T,Kimura K,Sumiya T,Nikaidou N,Suzuki K,Suzuki M,Taiyoji M,Ferrer S,Regue M

更新日期：1997-11-01 00:00:00

abstract：:The growth of tubercle bacilli in serum samples of untreated animals depends upon the availability of ionic iron which serves as a growth factor in supporting bacillary multiplication. The amount of available iron in serum is determined by the ratio between iron-saturated and iron-free transferrin; a low value for the...

journal_title：Journal of bacteriology

authors： Kochan I,Golden CA,Bukovic JA

更新日期：1969-10-01 00:00:00

abstract：:A temperate bacteriophage that determines the expression of enterohemolysin was isolated from Escherichia coli O26 strain C3888. The genetic determinant associated with enterohemolysin production (E-Hly determinant) was cloned from EcoRI-digested bacteriophage DNA in vector plasmid pUC8. pUC8 recombinant plasmid pEO19...

journal_title：Journal of bacteriology

authors： Beutin L,Bode L,Ozel M,Stephan R

更新日期：1990-11-01 00:00:00

abstract：:Two acid-inducible genes, aniC and aciK, that require anaerobiosis and tyrosine for expression were identified as orf326a encoding a potential amino acid/polyamine antiporter and hyaB encoding hydrogenase I, respectively. Cyclic AMP (cAMP) receptor protein, cAMP, and TyrR, regulator of aromatic amino acid metabolism, ...

journal_title：Journal of bacteriology

authors： Park KR,Giard JC,Eom JH,Bearson S,Foster JW

更新日期：1999-01-01 00:00:00

abstract：:Replicating DNA molecules of the nonconjugative R plasmid RSF1010 (Smr Sur) were cleaved with the EcoRI restriction endonuclease and examined with the electron microscope. Results of this analysis indicated that replication is initiated from an origin located at about 19% of total genome size from one of the EcoRI end...

journal_title：Journal of bacteriology

authors： de Graaff J,Crosa JH,Heffron F,Falkow S

更新日期：1978-06-01 00:00:00

abstract：:The cell envelope of gram-negative bacteria is responsible for many important biological functions: it plays a structural role, it accommodates the selective transfer of material across the cell wall, it undergoes changes made necessary by growth and division, and it transfers information about the environment into th...

journal_title：Journal of bacteriology

authors： Vadillo-Rodriguez V,Beveridge TJ,Dutcher JR

更新日期：2008-06-01 00:00:00

abstract：UNLABELLED:The gene encoding a nonoxidative decarboxylase capable of catalyzing the transformation of 2-hydroxy-1-naphthoic acid (2H1NA) to 2-naphthol was identified, recombinantly expressed, and purified to homogeneity. The putative gene sequence of the decarboxylase (hndA) encodes a 316-amino-acid protein (HndA) with...

journal_title：Journal of bacteriology

authors： Pal Chowdhury P,Basu S,Dutta A,Dutta TK

更新日期：2016-05-27 00:00:00

abstract：:The type III secretion system (T3SS) encoded by Salmonella pathogenicity island 2 (SPI-2) is involved in systemic infection and intracellular replication of Salmonella enterica serovar Typhimurium. In this study, we investigated the function of SsaE, a small cytoplasmic protein encoded within the SPI-2 locus, which sh...

journal_title：Journal of bacteriology

authors： Miki T,Shibagaki Y,Danbara H,Okada N

更新日期：2009-11-01 00:00:00