Abstract:
:The effects of exogenous reducing agents on a number of biological properties of purified Chlamydia trachomatis LGV-434 and Chlamydia psittaci meningopneumonitis elementary bodies (EBs) have been examined in an attempt to identify in vitro correlates of early events in the differentiation of the infectious EB to the replicative cell type, the reticulate body (RB). Treatment of EBs with dithiothreitol elicited a number of changes normally associated with differentiation to the RB. EBs in the presence of 10 mM dithiothreitol displayed enhanced rates of [14C]glutamate oxidation, reduced infectivity, and decreased osmotic stability, and their Machiavello staining properties changed to those characteristic of the RB. A true differentiation of EB to RB did not take place under these conditions, since EBs treated in this manner and examined by transmission electron microscopy did not demonstrate increased size or decreased electron density as do isolated RBs. Additional studies were initiated to identify the macromolecules involved in this process. With polyacrylamide gel electrophoresis and immunoblotting procedures with monoclonal and polyclonal monospecific antibodies, the chlamydial major outer membrane protein was found to be the predominant component that varied under reducing versus nonreducing conditions. Furthermore, the extent of disulfide-mediated cross-linking of the major outer membrane protein varied between the infective and replicative forms of the C. trachomatis LGV-434 life cycle. Implications of disulfide interactions in the life cycle of chlamydiae are discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hackstadt T,Todd WJ,Caldwell HDdoi
10.1128/JB.161.1.25-31.1985subject
Has Abstractpub_date
1985-01-01 00:00:00pages
25-31issue
1eissn
0021-9193issn
1098-5530journal_volume
161pub_type
杂志文章abstract::The ability to restart broken DNA replication forks is essential across all domains of life. In Escherichia coli, the priA, priB, priC, and dnaT genes encode the replication restart proteins (RRPs) to accomplish this task. PriA plays a critical role in replication restart such that its absence reveals a dramatic pheno...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00267-17
更新日期:2017-08-08 00:00:00
abstract::The lipopolysaccharide (LPS) from Rhizobium trifolii 0403 was isolated at different stages of growth and was examined for its (i) ability to bind a white clover lectin (trifoliin A), (ii) immunochemical properties, and (iii) composition. There was significantly more binding of trifoliin A to purified LPS and cells in ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.148.2.697-711.1981
更新日期:1981-11-01 00:00:00
abstract::Thermococcus kodakarensis (formerly Thermococcus kodakaraensis) strains have been constructed with synthetic and natural DNA sequences, predicted to function as archaeal transcription terminators, identically positioned between a constitutive promoter and a beta-glycosidase-encoding reporter gene (TK1761). Expression ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00982-09
更新日期:2009-11-01 00:00:00
abstract::Methanogenesis from the non-physiological C1 donors thioproline, thiazolidine, hexamethylenetetramine, formaldehyde (HCHO), and HOCH2-S-coenzyme M (CoM) was catalyzed by cell extracts of Methanobacterium thermoautotrophicum under a hydrogen atmosphere. Tetrahydromethanopterin (H4MPT) and HS-CoM were required in the re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.2.696-701.1985
更新日期:1985-02-01 00:00:00
abstract::Ben-Hamida, Fakher (Washington University School of Medicine, St. Louis, Mo.), and David Schlessinger. Stability of beta-galactosidase messenger ribonucleic acid in Escherichia coli. J. Bacteriol. 90:1611-1616. 1965.-Synthesis of beta-galactosidase stops within several minutes when preinduced, permeaseless cultures ar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.6.1611-1616.1965
更新日期:1965-12-01 00:00:00
abstract::Mutations in sulA (sfiA) block the filamentation and death of capR (lon) mutants that occur after treatments that either damage DNA or inhibit DNA replication and thereby induce the SOS response. Previous sulA-lacZ gene fusion studies showed that sulA is transcriptionally regulated by the SOS response system (lexA/rec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.2.551-561.1984
更新日期:1984-05-01 00:00:00
abstract::Phosphoenolpyruvate (PEP) carboxykinase was identified to be the only C3-carboxylating enzyme in Alcaligenes eutrophus. The enzyme requires GDP or inosine diphosphate (GTP or inosine triphosphate) for activity. Pyruvate- and other PEP-dependent CO2-fixing enzyme activities were not detected, regardless of whether the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.1.167-172.1984
更新日期:1984-07-01 00:00:00
abstract::Mutants of Schizosaccharomyces pombe, which were resistant to concentrations of colcemid inhibitory to the wild-type parent, were not diminished in their permeability to colcemid or colchicine. They also did not modify colcemid to a detectable derivative or to a product that was incapable of binding to brain tubulin. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.1.198-201.1977
更新日期:1977-01-01 00:00:00
abstract::Nitroakridin 3582 (NA) formed complexes with native deoxyribonucleic acid (DNA) and with transfer ribonucleic acid (tRNA) species from Escherichia coli. Spectrophotometric titrations of NA with these nucleic acids produced numerical results from which nonlinear adsorption isotherms were derived. These curves indicated...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.3.1026-1033.1971
更新日期:1971-12-01 00:00:00
abstract::To carry out a genetic analysis of the degradation and utilization of chitin by Serratia marcescens 2170, various Tn5 insertion mutants with characteristic defects in chitinase production were isolated and partially characterized. Prior to the isolation of the mutants, proteins secreted into culture medium in the pres...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.22.7111-7117.1997
更新日期:1997-11-01 00:00:00
abstract::The exopolysaccharide of Pseudomonas marginalis HT041B has been characterized as a 1,3-linked galactoglucan in which galactose and glucose are in the alpha- and beta-anomeric configurations, respectively. The polysaccharide is substituted with pyruvate at the 4 and 6 positions of galactose and with succinic acid at ei...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.3.1760-1762.1989
更新日期:1989-03-01 00:00:00
abstract::Aggregative amoebae of Dictyostelium discoideum, D. mucoroides, D. purpureum, and D. rosarium react chemotactically to cyclic AMP (cAMP). We measured the chemotactic activity of 14 cAMP analogs and found that these four species have a similar sensitivity to chemical modifications of cAMP; this suggests that the cAMP r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.1.99-105.1982
更新日期:1982-01-01 00:00:00
abstract::Intact spheroplasts of the cyanobacterium Anacystis nidulans were found to oxidize various exogenous c-type cytochromes with concomitant proton extrusion. In the coupled state, H+/e stoichiometries close to 1 were measured, regardless of absolute reaction rates. It is concluded that the proton translocation observed i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.539-542.1983
更新日期:1983-01-01 00:00:00
abstract::In Caulobacter crescentus, progression through the cell cycle is governed by the periodic activation and inactivation of the master regulator CtrA. Two phosphorelays, each initiating with the histidine kinase CckA, promote CtrA activation by driving its phosphorylation and by inactivating its proteolysis. Here, we exa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00992-09
更新日期:2009-12-01 00:00:00
abstract::A RecA-independent chromosomal rearrangement in the upstream region of the streptolysin O (slo) gene of Streptococcus pyogenes which affects slo expression was identified. PCR analysis was used to demonstrate that this kind of rearrangement was found in several strains of different lineages. Chromosomal loci involved ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.6.1857-1869.2003
更新日期:2003-03-01 00:00:00
abstract::Parnas, Józef (Academy of Medicine, Lublin, Poland). Differentiation of brucellae by the aid of phages. J. Bacteriol. 82:319-325. 1961.-There appears to be no absolute specificity of phages for the three species of the genus Brucella. The phage test is only supplementary to biochemical and serological methods for spec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.3.319-325.1961
更新日期:1961-09-01 00:00:00
abstract::Transposon mutagenesis was used to isolate mutants of Aeromonas hydrophila which were deficient in the production of extracellular proteins. The culture supernatants of two of the mutants were essentially devoid of the proteins normally secreted by the parent strain, despite their continued synthesis. Western immunobl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.3.1241-1249.1991
更新日期:1991-02-01 00:00:00
abstract::In contrast to previous findings, we demonstrate that the dissimilatory (bi)sulfite reductase genes (dsrAB) of Desulfobacula toluolica were vertically inherited. Furthermore, Desulfobacterium anilini and strain mXyS1 were identified, by dsrAB sequencing of 17 reference strains, as members of the donor lineage for thos...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.6.2203-2208.2005
更新日期:2005-03-01 00:00:00
abstract::The main chain of teichoic acids can be assembled in cell-free membrane preparations by the transfer of residues from the appropriate nucleotide precursors to an incompletely characterized amphiphilic molecule, lipoteichoic acid carrier (LTC). However, in the cell wall, the main chain is attached to peptidoglycan thro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.3.1222-1231.1981
更新日期:1981-03-01 00:00:00
abstract::Recipient (F(-)) cells of Escherichia coli are sensitive to an excess of Hfr donor cells. This phenomenon of lethal zygosis is associated with conjugation and is observed as a continuous fall in F(-) viable cells during liquid mating, or as inhibition of F(-) growth on solid media. One class of survivors, which arose ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.1.100-106.1974
更新日期:1974-01-01 00:00:00
abstract::Cholera, a severe diarrheal disease, is caused by ingestion of the gram-negative bacterium Vibrio cholerae. Expression of V. cholerae virulence factors is highly regulated at the transcriptional and posttranscriptional levels by a complex network of proteins and small noncoding RNAs. The direct activator of transcript...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00512-08
更新日期:2008-12-01 00:00:00
abstract::Molybdenum (Mo) is an important trace element that is toxic at high concentrations. To resolve the mechanisms underlying Mo toxicity, Rhodobacter capsulatus mutants tolerant to high Mo concentrations were isolated by random transposon Tn5 mutagenesis. The insertion sites of six independent isolates mapped within the s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00742-10
更新日期:2010-11-01 00:00:00
abstract::We measured some of the kinetic parameters of nitrogenase to intact systems of Clostridium pasteurianum and Klebsiella pneumoniae to compare them with the kinetics of the enzyme in vitro. We found that the enzyme showed multiple apparent Km values for acetylene reduction in vivo, as it does in vitro. Carbon monoxide w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.3.1230-1238.1980
更新日期:1980-03-01 00:00:00
abstract::Reticulate bodies of the meningopneumonitis (MP) microorganism were purified from L cells 18 hr after infection by the combination of differential centrifugation in 30% sucrose solution and potassium tartrate density gradient centrifugation. It was ascertained by electron microscopy that purified preparations of retic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.6.2003-2008.1967
更新日期:1967-06-01 00:00:00
abstract::The biosynthesis of asparaginase II in Saccharomyces cerevisiae is subject to nitrogen catabolite repression. In the present study we examined the physiological effects of glutamate auxotrophy on cellular metabolism and on the nitrogen catabolite repression of asparaginase II. Glutamate auxotrophic cells, incubated wi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.29-35.1982
更新日期:1982-07-01 00:00:00
abstract::The copy number of 2 mu DNA-derived plasmids in CIR+ Saccharomyces cerevisiae transformants is determined by its selective marker and is usually much lower than that of the endogenous plasmid. Only plasmids containing the leu2 allele of pJDB219, designated as leu2-d, under selective conditions displayed a higher copy ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.2.625-635.1983
更新日期:1983-11-01 00:00:00
abstract::The last step in proline biosynthesis in Escherichia coli K-12, Salmonella typhimurium LT7, and a number of other enterobacterial isolates is regulated so that no proline is excreted, even if excess Delta(1)-pyrroline-5-carboxylate, the immediate precursor of proline, is added to a culture. In proline auxotrophs block...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.3.928-934.1974
更新日期:1974-06-01 00:00:00
abstract::Elder, Robert L. (Johns Hopkins University, Baltimore, Md.), and Roland F. Beers, Jr. Nonphotoreactivating repair of ultraviolet light-damaged Micrococcus lysodeikticus cells. J. Bacteriol. 89:1225-1230. 1965.-Micrococcus lysodeikticus cells are highly resistant to ultraviolet radiation. The shape of the dose-survival...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.5.1225-1230.1965
更新日期:1965-05-01 00:00:00
abstract::The ability to respond to ambient pH is critical to the growth and virulence of the fungal pathogen Candida albicans. This response entails the differential expression of several genes affecting morphogenesis. To investigate the mechanism of pH-dependent gene expression, the C. albicans homolog of pacC, designated PRR...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.24.7524-7530.1999
更新日期:1999-12-01 00:00:00
abstract::The RpoS sigma factor (σ(S)) is the master regulator of the bacterial response to a variety of stresses. Mutants in rpoS arise in bacterial populations in the absence of stress, probably as a consequence of a subtle balance between self-preservation and nutritional competence. We characterized here one natural rpoS mu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00801-10
更新日期:2010-12-01 00:00:00