Abstract:
:We report the use of a fusion to the green fluorescent protein to visualize the assembly of the morphogenetic protein SpoIVA around the developing forespore during the process of sporulation in the bacterium Bacillus subtilis. Using a deconvolution algorithm to process digitally-collected optical sections, we show that SpoIVA, which is synthesized in the mother cell chamber of the sporangium, assembled into a spherical shell around the outer surface of the forespore. Time-lapse fluorescence microscopy showed that this assembly process commenced at the time of polar division and seemed to continue after engulfment of the forespore was complete. SpoIVA remained present throughout the late stages of morphogenesis and was present as a component of the fully mature spore. Evidence indicates that assembly of SpoIVA depended on the extreme C-terminal region of the protein and an additional region that directly or indirectly facilitated interaction among SpoIVA molecules. The N- and C-terminal regions of SpoIVA, including the extreme C terminus, are highly similar to the corresponding regions of the homologous protein from the distantly related endospore-forming bacterium Clostridium acetobutylicum, attesting to their importance in the function of the protein. Finally, we show that proper localization of SpoIVA required the expression of one or more genes which, like spoIVA, are under the control of the mother cell transcription factor sigmaE. One such gene was spoVM, whose product was required for efficient targeting of SpoIVA to the outer surface of the forespore.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Price KD,Losick Rdoi
10.1128/JB.181.3.781-790.1999subject
Has Abstractpub_date
1999-02-01 00:00:00pages
781-90issue
3eissn
0021-9193issn
1098-5530journal_volume
181pub_type
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/jb.178.10.2825-2835.1996
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.2.758-769.1980
更新日期:1980-02-01 00:00:00
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doi:10.1128/JB.147.1.57-61.1981
更新日期:1981-07-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1972-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1969-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.3.1115-1123.1975
更新日期:1975-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2001-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.176-181.1975
更新日期:1975-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.12.3723-3729.1994
更新日期:1994-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.188.5.1892-1898.2006
更新日期:2006-03-01 00:00:00
abstract::The YycFG two-component system is the only signal transduction system in Bacillus subtilis known to be essential for cell viability. This system is highly conserved in low-G+C gram-positive bacteria, regulating important processes such as cell wall homeostasis, cell membrane integrity, and cell division. Four other ge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-04-01 00:00:00
abstract::ICE6013 represents one of two families of integrative conjugative elements (ICEs) identified in the pan-genome of the human and animal pathogen Staphylococcus aureus Here we investigated the excision and conjugation functions of ICE6013 and further characterized the diversity of this element. ICE6013 excision was not ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2017-03-28 00:00:00
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doi:10.1128/jb.170.4.1752-1758.1988
更新日期:1988-04-01 00:00:00
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更新日期:1979-11-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2006-11-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2012-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1979-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1998-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.12.4071-4074.1997
更新日期:1997-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.7.2124-2131.1999
更新日期:1999-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1984-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.4.1060-1064.2004
更新日期:2004-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.2.616-619.1975
更新日期:1975-08-01 00:00:00