Growth-linked instability of a mutant valyl-transfer ribonucleic acid synthetase in Escherichia coli.

Abstract:

:The valyl-transfer ribonucleic acid (tRNA) synthetase of Escherichia coli strain NP2907, previously described as having an elevated K(m) for adenosine triphosphate and reduced stability in vitro compared to the wild type, was found to be conditionally thermolabile in vivo. The rate of inactivation of this enzyme at a particular temperature appears to be coordinated with the rate of growth; at 40 C this coordination results in equal rates of synthesis and destruction over a wide range of growth rates. In vitro studies showed that conditions favoring maintenance of the valyl-tRNA synthetase-valyl adenylate complex conferred complete protection against inactivation at 40 C, whereas the further addition of uncharged tRNA caused rapid, irreversible decay. We propose that the rate of inactivation of this mutant valyl-tRNA synthetase in vivo is a function of the ratio of deacylated to acylated tRNA(val) and that this ratio is a function of growth rate. The event which renders the valyl-tRNA synthetase susceptible to inactivation is likely to be the normal breakdown of the valyl-tRNA synthetase-valyl-adenylate complex during a cycle of aminoacylation of tRNA(val).

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Anderson JJ,Neidhardt FC

doi

10.1128/JB.109.1.315-325.1972

subject

Has Abstract

pub_date

1972-01-01 00:00:00

pages

315-25

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

109

pub_type

杂志文章
  • Differential regulation of multiple flagellins in Vibrio cholerae.

    abstract::Vibrio cholerae, the causative agent of the human diarrheal disease cholera, is a motile bacterium with a single polar flagellum. Motility has been implicated as a virulence determinant in some animal models of cholera, but the relationship between motility and virulence has not yet been clearly defined. We have begun...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.2.303-316.1998

    authors: Klose KE,Mekalanos JJ

    更新日期:1998-01-01 00:00:00

  • Investigation of two evolutionarily unrelated halocarboxylic acid dehalogenase gene families.

    abstract::Dehalogenases are key enzymes in the metabolism of halo-organic compounds. This paper describes a systematic approach to the isolation and molecular analysis of two families of bacterial alpha-halocarboxylic acid (alphaHA) dehalogenase genes, called group I and group II deh genes. The two families are evolutionarily u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.8.2535-2547.1999

    authors: Hill KE,Marchesi JR,Weightman AJ

    更新日期:1999-04-01 00:00:00

  • Regulatory roles for IscA and SufA in iron homeostasis and redox stress responses in the cyanobacterium Synechococcus sp. strain PCC 7002.

    abstract::SufA, IscA, and Nfu have been proposed to function as scaffolds in the assembly of Fe/S clusters in bacteria. To investigate the roles of these proteins further, single and double null-mutant strains of Synechococcus sp. strain PCC 7002 were constructed by insertional inactivation of genes homologous to sufA, iscA, an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.9.3182-3191.2006

    authors: Balasubramanian R,Shen G,Bryant DA,Golbeck JH

    更新日期:2006-05-01 00:00:00

  • Genome sequence of a recently emerged, highly transmissible, multi-antibiotic- and antiseptic-resistant variant of methicillin-resistant Staphylococcus aureus, sequence type 239 (TW).

    abstract::The 3.1-Mb genome of an outbreak methicillin-resistant Staphylococcus aureus (MRSA) strain (TW20) contains evidence of recently acquired DNA, including two large regions (635 kb and 127 kb). The strain is resistant to a wide range of antibiotics, antiseptics, and heavy metals due to resistance genes encoded on mobile ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01255-09

    authors: Holden MT,Lindsay JA,Corton C,Quail MA,Cockfield JD,Pathak S,Batra R,Parkhill J,Bentley SD,Edgeworth JD

    更新日期:2010-02-01 00:00:00

  • Synthesis of 1,3-beta-glucanases in Saccharomyces cerevisiae during the mitotic cycle, mating, and sporulation.

    abstract::Upon fractionating Saccharomyces cerevisiae asynchronous cultures by sucrose density gradient centrifugation in a zonal rotor and examining the exo-1,3-beta-glucanase and deoxyribonucleic acid content of the cells, a periodic step increase in the activity of this enzyme was observed, indicating a discontinuous pattern...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.3.924-931.1979

    authors: del Rey F,Santos T,García-Acha I,Nombela C

    更新日期:1979-09-01 00:00:00

  • Isolation in high frequency of rough variants of Mycobacterium intracellulare lacking C-mycoside glycopeptidolipid antigens.

    abstract::Rough variants of serovars from the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum complex were isolated in high frequency from pellicle growth of the wild-type strains. Rough morphology could be correlated with the lack of an outer cell wall sheath and its constituent C-mycoside glycopept...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.1.381-384.1982

    authors: Barrow WW,Brennan PJ

    更新日期:1982-04-01 00:00:00

  • Double-bond requirement in a fatty acid desaturase mutant of Saccharomyces cerevisiae.

    abstract::A fatty acid Delta(9)-desaturase mutant of yeast was analyzed to establish the specificity of the fatty acid structural requirement. Several double-bond-containing and substituted fatty acids were tested. It was concluded that the presence of a Delta(9)cis double bond is necessary for growth. The need for a specific c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.1.160-165.1970

    authors: Wisnieski BJ,Keith AD,Resnick MR

    更新日期:1970-01-01 00:00:00

  • Environmental role of pathogenic traits in Vibrio cholerae.

    abstract::Vibrio cholerae is a natural inhabitant of aquatic ecosystems. Some strains of V. cholerae can colonize the human host and cause cholera, a profuse watery diarrhea. The major pathogenicity factors and virulence regulators of V. cholerae are either encoded in mobile genetic elements acquired in the environment (e.g. pa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00795-17

    authors: Sakib SN,Reddi G,Almagro-Moreno S

    更新日期:2018-03-26 00:00:00

  • Glutathione plays a fundamental role in growth and symbiotic capacity of Sinorhizobium meliloti.

    abstract::Rhizobia form a symbiotic relationship with plants of the legume family to produce nitrogen-fixing root nodules under nitrogen-limiting conditions. We have examined the importance of glutathione (GSH) during free-living growth and symbiosis of Sinorhizobium meliloti. An S. meliloti mutant strain (SmgshA) which is unab...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.1.168-174.2005

    authors: Harrison J,Jamet A,Muglia CI,Van de Sype G,Aguilar OM,Puppo A,Frendo P

    更新日期:2005-01-01 00:00:00

  • Activity and transcriptional regulation of bacterial protein-like glycerol-3-phosphate dehydrogenase of the haloarchaea in Haloferax volcanii.

    abstract::Glycerol is a primary energy source for heterotrophic haloarchaea and a major component of "salty" biodiesel waste. Glycerol is catabolized solely by glycerol kinase (encoded by glpK) to glycerol-3-phosphate (G3P) in Haloferax volcanii. Here we characterized the next critical step of this metabolic pathway: the conver...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00276-11

    authors: Rawls KS,Martin JH,Maupin-Furlow JA

    更新日期:2011-09-01 00:00:00

  • Electron microscopic observations on the structure of the envelopes of mature elementary bodies and developmental reticulate forms of Chlamydia psittaci.

    abstract::Purified suspensions of Chlamydia psittaci were prepared from L cells. Thin sections of intact elementary bodies and intact developmental reticulate bodies and of their purified envelopes were observed by electron microscopy. In both intact organisms and partially purified envelopes, two membranous structures, each ap...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.1.355-360.1971

    authors: Tamura A,Matsumoto A,Manire GP,Higashi N

    更新日期:1971-01-01 00:00:00

  • Escherichia coli mutant with altered respiratory control of the frd operon.

    abstract::In wild-type Escherichia coli, fumarate reductase encoded by the frd operon is inducible by its substrate in the absence of molecular oxygen and nitrate. Synthesis of this enzyme under permissive conditions requires the fnr+ gene product, which is believed to be a pleiotropic regulatory protein that activates transcri...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.3.1023-1028.1985

    authors: Iuchi S,Kuritzkes DR,Lin EC

    更新日期:1985-03-01 00:00:00

  • Growth rate-dependent control of the rrnB P1 core promoter in Escherichia coli.

    abstract::We have extended our previous studies of the DNA sequences required for growth rate-dependent control of rRNA transcription in Escherichia coli. Utilizing a reporter system suitable for evaluation of promoters with low activities, we have found that the core promoter region of rrnB P1 (-41 to +1 with respect to the tr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.17.5560-5564.1994

    authors: Bartlett MS,Gourse RL

    更新日期:1994-09-01 00:00:00

  • Subcellular localization of a small sporulation protein in Bacillus subtilis.

    abstract::SpoVM is an unusually small (26-residue-long) protein that is produced in the mother cell chamber of the sporangium during the process of sporulation in Bacillus subtilis. We investigated the subcellular localization of SpoVM, which is believed to be an amphipathic alpha-helix, by using a fusion of the sporulation pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.4.1391-1398.2003

    authors: van Ooij C,Losick R

    更新日期:2003-02-01 00:00:00

  • Stimulation of menaquinone-dependent electron transfer in the respiratory chain of Bacillus subtilis by membrane energization.

    abstract::At a pH of

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.19.5339-5347.2002

    authors: Azarkina N,Konstantinov AA

    更新日期:2002-10-01 00:00:00

  • Micro-complement fixation in Klebsiella classification.

    abstract::The alkaline phosphatases of 29 strains of bacteria assigned by various authors to the genera Aerobacter, Klebsiella and Enterobacter were compared by the micro-complement fixation technique. On the basis of phosphatase resemblance, we recommend that all strains hitherto assigned to Aerobacter aerogenes and Enterobact...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.3.803-808.1972

    authors: Steffen DL,Cocks GT,Wilson AC

    更新日期:1972-06-01 00:00:00

  • Repair of extensive ionizing-radiation DNA damage at 95 degrees C in the hyperthermophilic archaeon Pyrococcus furiosus.

    abstract::We investigated the capacity of the hyperthermophile Pyrococcus furiosus for DNA repair by measuring survival at high levels of 60Co gamma-irradiation. The P. furiosus 2-Mb chromosome was fragmented into pieces ranging from 500 kb to shorter than 30 kb at a dose of 2,500 Gy and was fully restored upon incubation at 95...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.14.4643-4645.1997

    authors: DiRuggiero J,Santangelo N,Nackerdien Z,Ravel J,Robb FT

    更新日期:1997-07-01 00:00:00

  • Allantoate transport in Saccharomyces cerevisiae.

    abstract::Allantoate uptake appears to be mediated by an energy-dependent active transport system with an apparent Michaelis constant of about 50 microM. Cells were able to accumulate allantoate to greater than 3,000 times the extracellular concentration. The rate of accumulation was maximum at pH 5.7 to 5.8. The energy source ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.971-979.1979

    authors: Turoscy V,Cooper TG

    更新日期:1979-12-01 00:00:00

  • Nature of the complementation products formed by a complementing mutant of neurospora crassa.

    abstract::The mutant am-14 produces no active nicotinamide adenine dinucleotide phosphate-linked glutamate dehydrogenase (GDH) and no protein showing immunological cross-reaction with the enzyme. Nevertheless, it shows complementation with several other am mutants in heterokaryons. Active GDH can be extracted from heterokaryons...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.3.787-792.1968

    authors: Sundaram TK,Fincham JR

    更新日期:1968-03-01 00:00:00

  • Mating pheromones of Saccharomyces kluyveri: pheromone interactions between Saccharomyces kluyveri and Saccharomyces cerevisiae.

    abstract::Saccharomyces kluyveri is a heterothallic yeast with two allelic mating types denoted as a-k and alpha-k by analogy with Saccharomyces cerevisiae and from the work described here. S. kluyveri produces mating pheromones analogous to those of S. cerevisiae, but which appear to have different specificity. S. kluyveri thu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.1.146-154.1979

    authors: McCullough J,Herskowitz I

    更新日期:1979-04-01 00:00:00

  • Anaerobiosis induces expression of ant, a new Escherichia coli locus with a role in anaerobic electron transport.

    abstract::Escherichia coli has a formate hydrogenlyase system which allows it to maintain an electron balance during anaerobic growth by passing electrons from formate to H+ ions, thus generating H2. The Mu d1(Ap lac) bacteriophage was used to generate mutants that were defective in passing electrons from formate to benzyl viol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.1.180-186.1984

    authors: Yerkes JH,Casson LP,Honkanen AK,Walker GC

    更新日期:1984-04-01 00:00:00

  • Identification and characterization of genes determining receptor binding and pilus length of Escherichia coli type 1 pili.

    abstract::We describe the identification and characterization of two genes and their gene products responsible for determining receptor binding and pilus length in type 1-piliated Escherichia coli. One gene, pilE, conferred the ability of piliated cells to agglutinate guinea pig erythrocytes. The other gene, pilF, determined pi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.2.640-645.1987

    authors: Maurer L,Orndorff PE

    更新日期:1987-02-01 00:00:00

  • Overexpression of tnaC of Escherichia coli inhibits growth by depleting tRNA2Pro availability.

    abstract::Transcription of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and tryptophan-induced transcription antitermination. Induction results from ribosome stalling after translation of tnaC, the coding region for a 24-residue leader peptide. The last sense codon of tnaC, proline co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.5.1892-1898.2006

    authors: Gong M,Gong F,Yanofsky C

    更新日期:2006-03-01 00:00:00

  • Genome of Streptococcus oralis strain Uo5.

    abstract::Streptococcus oralis, a commensal species of the human oral cavity, belongs to the Mitis group of streptococci, which includes one of the major human pathogens as well, S. pneumoniae. We report here the first complete genome sequence of this species. S. oralis Uo5, a high-level penicillin- and multiple-antibiotic-resi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00321-11

    authors: Reichmann P,Nuhn M,Denapaite D,Brückner R,Henrich B,Maurer P,Rieger M,Klages S,Reinhard R,Hakenbeck R

    更新日期:2011-06-01 00:00:00

  • Cloning of the gene for myxobacterial hemagglutinin and isolation and analysis of structural gene mutations.

    abstract::Myxobacterial hemagglutinin (MBHA) is a major developmentally induced protein that accumulates during the period of cellular aggregation in the bacterium Myxococcus xanthus. It has been shown that this lectin is targeted to the cell surface and periplasmic space of developmental cells, suggesting that it may play a ro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.8.3801-3808.1987

    authors: Romeo JM,Zusman DR

    更新日期:1987-08-01 00:00:00

  • Sequence and transcriptional start site of the Pseudomonas aeruginosa outer membrane porin protein F gene.

    abstract::Porin F is one of the major proteins of the outer membrane of Pseudomonas aeruginosa. It forms water-filled pores of variable size. Porin F is a candidate for a vaccine against P. aeruginosa because it antigenically cross-reacts in all serotype strains of the International Antigenic Typing Scheme. We have isolated the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.1.155-162.1988

    authors: Duchêne M,Schweizer A,Lottspeich F,Krauss G,Marget M,Vogel K,von Specht BU,Domdey H

    更新日期:1988-01-01 00:00:00

  • Identification of rhtX and fptX, novel genes encoding proteins that show homology and function in the utilization of the siderophores rhizobactin 1021 by Sinorhizobium meliloti and pyochelin by Pseudomonas aeruginosa, respectively.

    abstract::Rhizobactin 1021 is a hydroxymate siderophore produced by the soil bacterium Sinorhizobium meliloti 2011. A regulon comprising rhtA, encoding the outer membrane receptor protein for the ferrisiderophore; the biosynthesis operon rhbABCDEF; and rhrA, the Ara-C-like regulator of the receptor and biosynthesis genes has be...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.10.2996-3005.2004

    authors: Cuív PO,Clarke P,Lynch D,O'Connell M

    更新日期:2004-05-01 00:00:00

  • Characterization of two genes encoding the Mycobacterium tuberculosis ribonucleotide reductase small subunit.

    abstract::Two nrdF genes, nrdF1 and nrdF2, encoding the small subunit (R2) of ribonucleotide reductase (RR) from Mycobacterium tuberculosis have 71% identity at the amino acid level and are both highly homologous with Salmonella typhimurium R2F. The calculated molecular masses of R2-1 and R2-2 are 36,588 (322 amino acids [aa]) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.20.6408-6415.1997

    authors: Yang F,Curran SC,Li LS,Avarbock D,Graf JD,Chua MM,Lu G,Salem J,Rubin H

    更新日期:1997-10-01 00:00:00

  • Inoculum-dependent division lag of Bacillus cultures and its relation to an endogenous factor(s) ("schizokinen").

    abstract::Lankford, C. E. (The University of Texas, Austin), James R. Walker, James B. Reeves, N. H. Nabbut, B. R. Byers, and R. J. Jones. Inoculum-dependent division lag of Bacillus cultures and its relation to an endogenous factor(s) ("schizokinen"). J. Bacteriol. 91:1070-1079. 1966.-When cells of Bacillus megaterium, grown o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.3.1070-1079.1966

    authors: Lankford CE,Walker JR,Reeves JB,Nabbut NH,Byers BR,Jones RJ

    更新日期:1966-03-01 00:00:00

  • The C-Terminal Zwitterionic Sequence of CotB1 Is Essential for Biosilicification of the Bacillus cereus Spore Coat.

    abstract:UNLABELLED:Silica is deposited in and around the spore coat layer of Bacillus cereus, and enhances the spore's acid resistance. Several peptides and proteins, including diatom silaffin and silacidin peptides, are involved in eukaryotic silica biomineralization (biosilicification). Homologous sequence search revealed a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00447-15

    authors: Motomura K,Ikeda T,Matsuyama S,Abdelhamid MA,Tanaka T,Ishida T,Hirota R,Kuroda A

    更新日期:2015-10-26 00:00:00