Abstract:
:Porin F is one of the major proteins of the outer membrane of Pseudomonas aeruginosa. It forms water-filled pores of variable size. Porin F is a candidate for a vaccine against P. aeruginosa because it antigenically cross-reacts in all serotype strains of the International Antigenic Typing Scheme. We have isolated the gene for porin F from a lambda EMBL3 bacteriophage library by using oligodeoxynucleotide hybridization probes and have determined its nucleotide sequence. Different peptide sequences obtained from isolated porin F confirmed the deduced protein sequence. The mature protein consists of 326 amino acid residues and has a molecular weight of 35,250. The precursor contains an N-terminal signal peptide of 24 amino acid residues. S1 protection and primer extension experiments, together with Northern (RNA) blots, indicate that the mRNA coding for porin F is monocistronic with short untranslated regions of about 58 bases at the 5' end and about 47 bases at the 3' end. The sequences in the -10 and -35 regions upstream of the transcriptional start site are closely related to the Escherichia coli promoter consensus sequences, which explains why the porin F gene is expressed in E. coli under the control of its own promoter. The amino acid sequence of porin F is not homologous to the different E. coli porins OmpF, OmpC, LamB, and PhoE. On the other hand, a highly homologous region of 30 amino acids between the OmpA proteins of different enteric bacteria and porin F of P. aeruginosa was detected. The core region of the homology to E. coli OmpA had 11 of 12 amino acid residues in common.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Duchêne M,Schweizer A,Lottspeich F,Krauss G,Marget M,Vogel K,von Specht BU,Domdey Hdoi
10.1128/jb.170.1.155-162.1988subject
Has Abstractpub_date
1988-01-01 00:00:00pages
155-62issue
1eissn
0021-9193issn
1098-5530journal_volume
170pub_type
杂志文章abstract::The ugp operon of Escherichia coli includes genes involved in the uptake of sn-glycerol-3-phosphate and glycerophosphoryl diesters and belongs to the pho regulon which is induced by phosphate limitation. This operon has two transcriptional initiation sites, as determined by S1 nuclease mapping of the in vivo transcrip...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.549-558.1991
更新日期:1991-01-01 00:00:00
abstract::The kinetics of beta-lactamase induction in Pseudomonas maltophilia IID1275/873 were investigated. Upon induction with beta-lactam antibiotics, a correlation was seen between the increase in specific beta-lactamase activity and the generation time, as well as the concentration of inducer in the medium. The specific be...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.1.483-487.1989
更新日期:1989-01-01 00:00:00
abstract::Macrocysts have been found among strains of Dictyostelium rosarium Raper and Cavender (K. B. Raper and J. C. Cavender, J. Elisha Mitchell Sci. Soc. 84:31-47, 1968). Strains that exhibit mating capabilities could be classified into one of three mating types: A1, A2, or A3. Each mating type was self-incompatible but cro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.3.1049-1053.1981
更新日期:1981-09-01 00:00:00
abstract::A computer program has been designed to study behavior in populations of Spirochaeta aurantia cells, and this program has been used to analyze changes in behavior in response to chemoattractants. Three kinds of behavior were distinguished: smooth swimming, flexing, and reversals in direction of swimming after a short ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1768-1774.1988
更新日期:1988-04-01 00:00:00
abstract::Cyanobacteria are monophyletic organisms that perform oxygenic photosynthesis. While they exhibit great diversity, they have a common set of genes. However, the essentiality of them for viability has hampered the elucidation of their functions. One example of these genes is cyabrB1 (also known as calA in Anabaena sp. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00244-19
更新日期:2019-08-08 00:00:00
abstract::trpG of Bacillus subtilis encodes a glutamine amidotransferase subunit that is involved in the synthesis of both folic acid and L-tryptophan. Expression of trpG is negatively regulated by tryptophan even though this gene is located within a folic acid biosynthetic operon. Examination of both transcriptional and transl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.15.4272-4278.1995
更新日期:1995-08-01 00:00:00
abstract::Two proteins that bind penicillin were observed in Escherichia coli infected with lambda phages 141, 142, 650, and 651 from the Kohara genomic library. These phages carry chromosomal DNA fragments that do not contain any known penicillin binding protein (PBP) genes, indicating that unrecognized gene products were exhi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.19.6112-6121.1997
更新日期:1997-10-01 00:00:00
abstract::The effect of heat shock on Myxococcus xanthus was investigated during both glycerol- and starvation-induced development. Cells heat shocked at 40 degrees C for 1 h prior to a development-inducing signal displayed an accelerated rate of myxospore formation at 30 degrees C. Additionally, M. xanthus cells heat shocked p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.11.5200-5207.1988
更新日期:1988-11-01 00:00:00
abstract::The ends of the bacterial transposon gamma delta contain adjacent binding sites for gamma delta transposase and integration host factor (IHF). IHF+ and IHF- strains were used in conjunction with gamma delta transposon ends containing or lacking the site for IHF binding to determine the role that IHF plays in various g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.4951-4958.1990
更新日期:1990-09-01 00:00:00
abstract::Bacteria often encounter environments where nutrient availability is limited, and they must adapt accordingly. To identify Pasteurella multocida genes that are differentially expressed during nutrient limitation, we utilized whole-genome microarrays to compare levels of gene expression during growth in rich and minima...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.13.3734-3739.2002
更新日期:2002-07-01 00:00:00
abstract::Shiga toxin (STX), a bacterial toxin produced by Shigella dysenteriae type 1, is a hexamer composed of five receptor-binding B subunits which encircle an alpha-helix at the carboxyl terminus of the enzymatic A polypeptide. Hybrid toxins constructed by fusing the A polypeptide sequences of STX and Shiga-like toxin type...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.11.3128-3132.1995
更新日期:1995-06-01 00:00:00
abstract::A newly isolated bacterium, Pseudomonas geniculata N1, can efficiently degrade nicotine. Here we present a 4.51-Mb assembly of its genome, which is the first sequence of the P. geniculata group. The sequence contains the genes related to nicotine catabolism and may provide insights into its molecular mechanism for N-h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00625-12
更新日期:2012-07-01 00:00:00
abstract::The SsuDAT1I restriction-modification (R-M) system, which contains two methyltransferases and two restriction endonucleases with recognition sequence 5'-GATC-3', was first found in a field isolate of Streptococcus suis serotype 2. Isoschizomers of the R-M system were found in the same locus between purH and purD in a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.183.18.5436-5440.2001
更新日期:2001-09-01 00:00:00
abstract::A temperate phage, phi CTX, is a cytotoxin-converting phage of Pseudomonas aeruginosa. In this study, we characterized the lipopolysaccharide (LPS) structures of phi CTX-resistant mutants derived from phi CTX-sensitive strains. phi CTX infectivity was neutralized by LPS preparations derived from sensitive strains but ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.17.5262-5269.1994
更新日期:1994-09-01 00:00:00
abstract::A new carboxypeptidase (carboxypeptidase S) was found in a Saccharomyces cerevisiae strain lacking carboxypeptidase Y (D. H. Wolf and U. Weiser, Eur. J. Biochem. 73:553-556, 1977). Mutants devoid of carboxypeptidase S activity were isolated from a mutant strain that was also deficient in carboxypeptidase Y. Four mutan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.2.418-426.1981
更新日期:1981-08-01 00:00:00
abstract::In growing cultures of Escherichia coli, the nonacylated glycerol of phosphatidylglycerol (PG) is labeled more rapidly than is the acylated glycerol. This is, in part, due to a rapid exchange reaction of the nonacylated glycerol. Only some of the PG molecules undergo this reaction while others are stable. Using a muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.1.210-214.1973
更新日期:1973-10-01 00:00:00
abstract::Amino acid transport rates and amino acid binding proteins were examined in a strain containing the rho-120 mutation (formerly SuA), which has been shown to lower the rho-dependent, ribonucleic acid-activated adenosine triphosphatase activity to 9% of the rho activity in the isogenic wild-type strain. Tryptophan and p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.3.1024-1029.1977
更新日期:1977-06-01 00:00:00
abstract::Temperature-sensitive nalA mutants of Escherichia coli have been used to investigate the structure and functions of deoxyribonucleic acid (DNA) gyrase. Extracts of one such mutant (nalA43) had thermosensitive DNA gyrase subunit A activity but normal gyrase subunit B activity, proving definitively that nalA is the stru...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.424-435.1979
更新日期:1979-11-01 00:00:00
abstract::Resting cells of Escherichia coli are able to initiate growth and murein biosynthesis in the presence of beta-lactam antibiotics binding to penicillin-binding proteins (PBPs) 1a and 1b (E. J. de la Rosa, M. A. de Pedro, and D. Vázquez, Proc. Natl. Acad. Sci. USA 82:5632-5635, 1985). Under these conditions, cells elong...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2410-2416.1987
更新日期:1987-06-01 00:00:00
abstract::Beggs, William H. (University of Minnesota, Minneapolis), and Palmer Rogers. Galactose repression of beta-galactosidase induction in Escherichia coli. J. Bacteriol. 91:1869-1874. 1966.-Galactose repression of beta-galactosidase induction in Escherichia coli was investigated to determine whether the galactose molecule ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.5.1869-1874.1966
更新日期:1966-05-01 00:00:00
abstract::Biphenyl metabolism in Aspergillus toxicarius occurs by successive hydroxylations in the 4- and 4'-positions, followed by conjugation with sulfate to produce 4-hydroxybiphenyl-O-sulfonic acid and 4,4'-dihydroxybiphenyl-O-sulfonic acid. The hydroxylation reactions normally occur only after a prolonged lag period after ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.49-57.1983
更新日期:1983-10-01 00:00:00
abstract::Adler, H. E. (University of California, Davis), M. Shifrine, and Herrad Ortmayer. Mycoplasma inocuum sp. n., a saprophyte from chickens. J. Bacteriol. 82:239-240. 1961.-A new species of Mycoplasma was isolated from the infraorbital sinuses of chickens. The organism, a saprophyte, differs from all known mycoplasmas of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.2.239-240.1961
更新日期:1961-08-01 00:00:00
abstract::NAD-independent lactate dehydrogenases are commonly thought to be responsible for lactate utilization during the stationary phase of aerobic growth in Lactobacillus plantarum. To substantiate this view, we constructed single and double knockout mutants for the corresponding genes, loxD and loxL. Lactate-to-acetate con...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.19.6661-6666.2004
更新日期:2004-10-01 00:00:00
abstract::Cyclic nucleotide-independent protein kinase (EC 2.7.1.37) activity was found in the nuclear cap organelle, within which ribosomes of zoospores of Blastocladiella emersonii are sequestered. Two protein kinase activities were resolved from the high-salt wash fraction of zoospore ribosomes by selective adsorption to DEA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.3.1467-1471.1983
更新日期:1983-06-01 00:00:00
abstract::In gram-negative organisms, enzymes belonging to the low-molecular-weight protein tyrosine phosphatase (LMPTP) family are involved in the regulation of important physiological functions, including stress resistance and synthesis of the polysaccharide capsule. LMPTPs have been identified also in gram-positive bacteria,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.14.4945-4956.2005
更新日期:2005-07-01 00:00:00
abstract::S-Adenosyl-L-methionine:precorrin-2 methyltransferase (SP2MT), which catalyzes the C-20 methylation of precorrin-2 to precorrin-3, was purified to homogeneity from extracts of a recombinant strain of Pseudomonas denitrificans derived from a cobalamin-overproducing strain. Ammonium sulfate fractionation followed by chr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.11.6245-6251.1990
更新日期:1990-11-01 00:00:00
abstract::The structural gene for the F sex factor outer membrane surface exclusion protein ( traT ) was cloned onto a high-copy-number plasmid where it is expressed from the phage lambda promoter pL. Conditional control over expression was provided by a temperature-sensitive lambda cI repressor. Induction of pL produced large ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.2.464-473.1984
更新日期:1984-05-01 00:00:00
abstract::Although nitrate stimulated the capacity of Clostridium thermoautotrophicum and Clostridium thermoaceticum to oxidize (utilize) substrates under heterotrophic conditions, it inhibited autotrophic H2-CO2-dependent growth. Under basal medium conditions, nitrate was also inhibitory to the use of one-carbon substrates (i....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.15.4597-4603.1996
更新日期:1996-08-01 00:00:00
abstract::The kinetics of peptidoglycan degradation were examined under different conditions of autolysis of Escherichia coli. With cephaloridine- or moenomycin-induced autolysis, degradation did not exceed 25 to 35%, whereas in EDTA-induced autolysis it rapidly reached 65 to 70%. When nonautolyzing cells were fixed overnight w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.2.627-635.1985
更新日期:1985-02-01 00:00:00
abstract::Heterocyst differentiation is orchestrated by the N control transcriptional regulator NtcA and the differentiation-specific factor HetR. In Anabaena sp. strain PCC 7120, the devBCA operon is expressed from two different promoters activated upon N stepdown. The distal devB promoter (transcription start point [TSP] loca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05994-11
更新日期:2012-06-01 00:00:00