Analysis of Shiga toxin subunit association by using hybrid A polypeptides and site-specific mutagenesis.

abstract：:The morphological and physiological characteristics of 20 isolates of psychrophilic Bacillus were compared with 29 strains representing nine species of mesophilic Bacillus and 2 strains of Sporosarcina ureae to determine the taxonomic position of the psychrophiles. The psychrophiles formed four distinct groups which w...

journal_title：Journal of bacteriology

authors： Larkin JM,Stokes JL

更新日期：1967-10-01 00:00:00

abstract：:An 846-bp cryptic plasmid has been discovered in the hyperthermophilic bacterium Thermotoga sp. strain RQ7. This is the first plasmid described for an organism from this ancient bacterial lineage and the smallest plasmid described to date for any organism. Nucleotide sequencing revealed a single open reading frame pos...

journal_title：Journal of bacteriology

authors： Harriott OT,Huber R,Stetter KO,Betts PW,Noll KM

更新日期：1994-05-01 00:00:00

abstract：:The antibacterial protein Colicin V (ColV) is secreted from gram-negative bacteria by a signal sequence-independent pathway. The proteins that mediate the export of ColV share sequence similarities with components from other signal sequence-independent export systems such as those for alpha-hemolysin (Hly) and Erwinia...

journal_title：Journal of bacteriology

authors： Fath MJ,Skvirsky RC,Kolter R

更新日期：1991-12-01 00:00:00

abstract：:A hypothesis that preferential rejection of donor markers by the hex system of pneumococcus is due to lethal double-strand breaks has been examined in terms of its implications for the extent of the excision required. Experiments reported here were directed at asking whether hex-dependent marker efficiency depends on ...

journal_title：Journal of bacteriology

authors： Guild WR,Shoemaker NB

更新日期：1976-01-01 00:00:00

abstract：:Rhizobium sp. strain NGR234 produces a large family of lipochitooligosaccharide Nod factors carrying specific substituents. Among them are 3-O- (or 4-O-) and 6-O-carbamoyl groups, an N-methyl group, and a 2-O-methylfucose residue which may bear either 3-O-sulfate or 4-O-acetyl substitutions. Investigations on the gene...

journal_title：Journal of bacteriology

authors： Quesada-Vincens D,Fellay R,Nasim T,Viprey V,Burger U,Prome JC,Broughton WJ,Jabbouri S

更新日期：1997-08-01 00:00:00

abstract：:The opportunistic bacterial pathogen Vibrio vulnificus causes severe wound infection and fatal septicemia. We used alkaline phosphatase insertion mutagenesis in a clinical isolate of V. vulnificus to find genes necessary for virulence, and we identified fadR, which encodes a regulator of fatty acid metabolism. The fad...

journal_title：Journal of bacteriology

authors： Brown RN,Gulig PA

更新日期：2008-12-01 00:00:00

abstract：:Type VI secretion systems (T6SS) are bacteriophage-derived macromolecular machines responsible for the release of at least two proteins in the milieu, which are thought to form an extracellular appendage. Although several T6SS have been shown to be involved in the virulence of animal and plant pathogens, clusters enco...

journal_title：Journal of bacteriology

authors： Bernard CS,Brunet YR,Gavioli M,Lloubès R,Cascales E

更新日期：2011-05-01 00:00:00

abstract：:wbdA is a mannosyltransferase gene that is involved in synthesis of the Escherichia coli O9a polysaccharide, a mannose homopolymer with a repeating unit of 2-alphaMan-1,2-alphaMan-1,3-alphaMan-1, 3-alphaMan-1. The equivalent structural O polysaccharide in the E. coli O9 and Klebsiella O3 strains is 2-alphaMan-1,2-alph...

journal_title：Journal of bacteriology

authors： Kido N,Kobayashi H

更新日期：2000-05-01 00:00:00

abstract：:Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents (B. Demple and S. Linn, J. Biol. Chem. 257:2848-2855, 1982). Thus, it may be a versatile DNA repair enzyme. The protein was purified to apparent homogeneity, and from its N-terminal seque...

journal_title：Journal of bacteriology

authors： Guo G,Ding Y,Weiss B

更新日期：1997-01-01 00:00:00

abstract：:Pantothenate kinase (CoaA) is a key regulator of coenzyme A (CoA) biosynthesis in Escherichia coli, and its activity is controlled by feedback inhibition by CoA and its thioesters. The importance of feedback inhibition in the control of the intracellular CoA levels was tested by constructing three site-directed mutant...

journal_title：Journal of bacteriology

authors： Rock CO,Park HW,Jackowski S

更新日期：2003-06-01 00:00:00

abstract：:Isotopic exchange of (35)S between penicillins and 6-amino-penicillanic acid (6-APA) was observed in cell-free extracts of Penicillium chrysogenum. Sulfhydryl-containing compounds were required for activity. Dithiothreitol, dithioerythritol, mercaptoethanol, and glutathione served as activators. The acyltransferase wa...

journal_title：Journal of bacteriology

authors： Pruess DL,Johnson MJ

更新日期：1967-11-01 00:00:00

abstract：:Cleared lysates of 13 species of filamentous cyanobacteria were examined for the presence of extrachromosomal DNA by using agarose gel electrophoresis and ethidium bromide staining. Seven of the 13 species contained extrachromosomal covalently closed circular DNA, and all but 1 species contained multiple elements. The...

journal_title：Journal of bacteriology

authors： Simon RD

更新日期：1978-10-01 00:00:00

abstract：:Human-pathogenic Bartonella henselae causes cat scratch disease and vasculoproliferative disorders. An important pathogenicity factor of B. henselae is the trimeric autotransporter adhesin (TAA) Bartonella adhesin A (BadA), which is modularly constructed, consisting of a head, a long and repetitive neck-stalk module, ...

journal_title：Journal of bacteriology

authors： Schmidgen T,Kaiser PO,Ballhorn W,Franz B,Göttig S,Linke D,Kempf VA

更新日期：2014-06-01 00:00:00

abstract：:Lipopolysaccharide, lipooligosaccharide (LOS), or endotoxin is important in bacterial survival and the pathogenesis of gram-negative bacteria. A necessary step in endotoxin biosynthesis is 3-deoxy-D-manno-octulosonic acid (Kdo) glycosylation of lipid A, catalyzed by the Kdo transferase KdtA (WaaA). In enteric gram-neg...

journal_title：Journal of bacteriology

authors： Tzeng YL,Datta A,Kolli VK,Carlson RW,Stephens DS

更新日期：2002-05-01 00:00:00

abstract：:Salmonella typhimurium LT2 normally expresses two acetohydroxy acid synthases (AHAS I and AHAS II). The function of AHAS I in this organism was unclear, since AHAS I-deficient (ilvBN) mutants of LT2 grew well on glucose or succinate minimal media, whereas AHAS II-deficient (ilvGM) mutants requried isoleucine for norma...

journal_title：Journal of bacteriology

authors： Dailey FE,Cronan JE Jr,Maloy SR

更新日期：1987-02-01 00:00:00

abstract：:A new pathway for aerobic benzoate oxidation has been postulated for Azoarcus evansii and for a Bacillus stearothermophilus-like strain. Benzoate is first transformed into benzoyl coenzyme A (benzoyl-CoA), which subsequently is oxidized to 3-hydroxyadipyl-CoA and then to 3-ketoadipyl-CoA; all intermediates are CoA thi...

journal_title：Journal of bacteriology

authors： Gescher J,Zaar A,Mohamed M,Schägger H,Fuchs G

更新日期：2002-11-01 00:00:00

abstract：:PacI and SpeI restriction maps were obtained for the two chromosomes of each of the six species of the genus Brucella: B. melitensis, B. abortus, B. suis, B. canis, B. ovis, and B. neotomae. Three complementary techniques were used: hybridization with the two replicons as probes, cross-hybridization of restriction fra...

journal_title：Journal of bacteriology

authors： Michaux-Charachon S,Bourg G,Jumas-Bilak E,Guigue-Talet P,Allardet-Servent A,O'Callaghan D,Ramuz M

更新日期：1997-05-01 00:00:00

abstract：:Tryptophan is an essential amino acid that is required for normal development in Chlamydia species, and tryptophan metabolism has been implicated in chlamydial persistence and tissue tropism. The ability to synthesize tryptophan is not universal among the Chlamydiaceae, but species that have a predicted tryptophan bio...

journal_title：Journal of bacteriology

authors： Akers JC,Tan M

更新日期：2006-06-01 00:00:00

abstract：:The structural properties required for the binding of peptide substrates to the Escherichia coli periplasmic protein involved in oligopeptide transport were surveyed by measuring the ability of different peptides to compete for binding in an equilibrium dialysis assay with the tripeptide Ala-Phe-[3H]Gly. The protein s...

journal_title：Journal of bacteriology

authors： Guyer CA,Morgan DG,Staros JV

更新日期：1986-11-01 00:00:00

abstract：:Experiments were performed to characterize the pyrogenic principle of ribonucleic acid (RNA) from the yeast Candida utilis. It was shown that ribonuclease hydrolysis of the RNA does not lead to inactivation of the pyrogenicity. Pyrogenicity was, however, destroyed by treatment with sodium deoxycholate. On column chrom...

journal_title：Journal of bacteriology

authors： Kanoh S,Kohlhage H,Siegert R

更新日期：1968-09-01 00:00:00

abstract：:Lipid synthesis was studied in both sporulating (diploid) and nonsporulating (haploid) cells of Saccharomyces cerevisiae. Two phases of lipid synthesis occur in diploid cells transferred to sporulation medium. Phase I, which occurs during the first 12 h of exposure to sporulation medium, was also observed in the haplo...

journal_title：Journal of bacteriology

authors： Henry SA,Halvorson HO

更新日期：1973-06-01 00:00:00

abstract：:Micromonospora strains have been isolated from diverse niches, including soil, water, and marine sediments and root nodules of diverse symbiotic plants. In this work, we report the genome sequence of Micromonospora lupini Lupac 08 isolated from root nodules of the wild legume Lupinus angustifolious. ...

journal_title：Journal of bacteriology

authors： Alonso-Vega P,Normand P,Bacigalupe R,Pujic P,Lajus A,Vallenet D,Carro L,Coll P,Trujillo ME

更新日期：2012-08-01 00:00:00

abstract：:Day, L. E. (Chas. Pfizer & Co., Inc., Groton, Conn.). Tetracycline inhibition of cell-free protein synthesis. II. Effect of the binding of tetracycline to the components of the system. J. Bacteriol. 92:197-203. 1966.-When tetracycline, an inhibitor of cell-free protein synthesis, was preincubated with each component o...

journal_title：Journal of bacteriology

authors： Day LE

更新日期：1966-07-01 00:00:00

abstract：:The chlorosome envelope of Chlorobaculum tepidum contains 10 proteins that belong to four structural motif families. A previous mutational study (N.-U. Frigaard, H. Li, K. J. Milks, and D. A. Bryant, J. Bacteriol. 186:646-653, 2004) suggested that some of these proteins might have redundant functions. Six multilocus m...

journal_title：Journal of bacteriology

authors： Li H,Bryant DA

更新日期：2009-11-01 00:00:00

abstract：:The effect of heat shock on Myxococcus xanthus was investigated during both glycerol- and starvation-induced development. Cells heat shocked at 40 degrees C for 1 h prior to a development-inducing signal displayed an accelerated rate of myxospore formation at 30 degrees C. Additionally, M. xanthus cells heat shocked p...

journal_title：Journal of bacteriology

authors： Killeen KP,Nelson DR

更新日期：1988-11-01 00:00:00

abstract：:Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunological techniques, we have compared the synthesis of the phoA protein (alkaline phosphatase) and the phoS protein (phosphate-binding protein) in response to the level of phosphate in the medium in different genetic backgrounds containing the kn...

journal_title：Journal of bacteriology

authors： Willsky GR,Malamy MH

更新日期：1976-07-01 00:00:00

abstract：:Bradyrhizobium diazoefficiens is a soil alphaproteobacterium that possesses two evolutionarily distinct flagellar systems, a constitutive subpolar flagellum and inducible lateral flagella that, depending on the carbon source, may be expressed simultaneously in liquid medium and used interactively for swimming. In each...

journal_title：Journal of bacteriology

authors： Mengucci F,Dardis C,Mongiardini EJ,Althabegoiti MJ,Partridge JD,Kojima S,Homma M,Quelas JI,Lodeiro AR

更新日期：2020-02-11 00:00:00

abstract：:Kimler, Alexander (National Institutes of Health, Bethesda, Md.). Fluorescein amine media for rapid differentiation of staphylococci. J. Bacteriol. 82:106-109. 1961.-By modifying conventional media to include blood, mannitol, and fluorescein amine, staphylococci may be isolated and identified directly. Staphylococcus ...

journal_title：Journal of bacteriology

authors： KIMLER A

更新日期：1961-07-01 00:00:00

abstract：:Halocin S8 is a hydrophobic microhalocin of 36 amino acids (3,580 Da) and is the first microhalocin to be described. This peptide antibiotic is unique since it is processed from inside a much larger, 33,962-Da pro-protein. Halocin S8 is quite robust, as it can be desalted, boiled, subjected to organic solvents, and st...

journal_title：Journal of bacteriology

authors： Price LB,Shand RF

更新日期：2000-09-01 00:00:00

abstract：:The gltA gene, encoding Sinorhizobium meliloti 104A14 citrate synthase, was isolated by complementing an Escherichia coli gltA mutant. The S. meliloti gltA gene was mutated by inserting a kanamycin resistance gene and then using homologous recombination to replace the wild-type gltA with the gltA::kan allele. The resu...

journal_title：Journal of bacteriology

authors： Mortimer MW,McDermott TR,York GM,Walker GC,Kahn ML

更新日期：1999-12-01 00:00:00