A cryptic miniplasmid from the hyperthermophilic bacterium Thermotoga sp. strain RQ7.

Abstract:

:An 846-bp cryptic plasmid has been discovered in the hyperthermophilic bacterium Thermotoga sp. strain RQ7. This is the first plasmid described for an organism from this ancient bacterial lineage and the smallest plasmid described to date for any organism. Nucleotide sequencing revealed a single open reading frame possibly encoding a 25,460-Da basic protein (212 amino acids). Upstream of the putative promoter lie five 11-bp direct repeats, each separated by 1 to 4 bp, while between the promoter and the open reading frame lies an 11-bp palindromic sequence. Its mode of replication is unknown, but its sequence bears similarities to those of plasmids which replicate by a rolling-circle mechanism.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Harriott OT,Huber R,Stetter KO,Betts PW,Noll KM

doi

10.1128/jb.176.9.2759-2762.1994

subject

Has Abstract

pub_date

1994-05-01 00:00:00

pages

2759-62

issue

9

eissn

0021-9193

issn

1098-5530

journal_volume

176

pub_type

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