Glycosylation is required for outer membrane localization of the lectin LecB in Pseudomonas aeruginosa.

abstract：:The sequence of the pckA gene coding for phosphoenolpyruvate carboxykinase in Escherichia coli K-12 and previous molecular weight determinations indicate that this allosteric enzyme is a monomer of Mr 51,316. The protein is homologous to ATP-dependent phosphoenolpyruvate carboxykinases from Trypanosoma brucei and Sacc...

journal_title：Journal of bacteriology

authors： Medina V,Pontarollo R,Glaeske D,Tabel H,Goldie H

更新日期：1990-12-01 00:00:00

abstract：:Herbaspirillum sp. strain GW103 was isolated from rhizosphere soil of the reed Phragmites australis on reclaimed land. Here we report the 5.05-Mb draft genome sequence of the strain, providing bioinformation about the agronomic benefits of this strain, such as multiple traits relevant to plant root colonization and pl...

journal_title：Journal of bacteriology

authors： Lee GW,Lee KJ,Chae JC

更新日期：2012-08-01 00:00:00

abstract：:Sinorhizobium meliloti, a gram-negative soil bacterium, forms a nitrogen-fixing symbiotic relationship with members of the legume family. To facilitate our studies of transcription in S. meliloti, we cloned and characterized the gene for the alpha subunit of RNA polymerase (RNAP). S. meliloti rpoA encodes a 336-amino-...

journal_title：Journal of bacteriology

authors： Peck MC,Gaal T,Fisher RF,Gourse RL,Long SR

更新日期：2002-07-01 00:00:00

abstract：:The virulence of Staphylococcus aureus is controlled by the accessory gene regulator (agr) system, including an extracellular inducer encoded by agrD. Variable agr PCR restriction fragment length polymorphism (RFLP) patterns of unique S. aureus strains (n = 192) were determined for a region comprising agrD and parts o...

journal_title：Journal of bacteriology

authors： van Leeuwen W,van Nieuwenhuizen W,Gijzen C,Verbrugh H,van Belkum A

更新日期：2000-10-01 00:00:00

abstract：UNLABELLED:To stabilize cellular integrity in the face of environmental perturbations, most bacteria, including cyanobacteria, synthesize and maintain a strong, flexible, three-dimensional peptidoglycan lattice. Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium capable of differentiating morphologically dist...

journal_title：Journal of bacteriology

authors： Videau P,Rivers OS,Ushijima B,Oshiro RT,Kim MJ,Philmus B,Cozy LM

更新日期：2016-03-31 00:00:00

abstract：:The plasmid-encoded epidermin biosynthetic gene epiC of Staphylococcus epidermidis Tü3298 was expressed in Escherichia coli by using the T7 RNA polymerase-promoter system, and the gene product EpiC was identified by Western blotting (immunoblotting) with an anti-EpiC-peptide antiserum. EpiC was a hydrophobic but solub...

journal_title：Journal of bacteriology

authors： Kupke T,Gotz F

更新日期：1996-03-01 00:00:00

abstract：:Klebsiella pneumoniae can use nitrate and nitrite as sole nitrogen sources through the nitrate assimilatory pathway. The structural genes for assimilatory nitrate and nitrite reductases together with genes necessary for nitrate transport form an operon, nasFEDCBA. Expression of the nasF operon is regulated both by gen...

journal_title：Journal of bacteriology

authors： Goldman BS,Lin JT,Stewart V

更新日期：1994-08-01 00:00:00

abstract：:A general, genetic technique for determining the direction of transcription for bacterial genes is presented. By comparing the phenotype of Mu-Mud1 dilysogens with the phenotype of deletion-containing derivatives, the direction of transcription for the gene containing Mud1 can be unambiguously determined. This method ...

journal_title：Journal of bacteriology

authors： MacNeil D

更新日期：1981-04-01 00:00:00

abstract：:Salmonella typhimurium was found to utilize 2-deoxyribose as a sole carbon and energy source. Cells grown in the presence of deoxyribose contained increased levels of deoxyribose kinase, thymidine phosphorylase, and two forms of deoxyribose-5-phosphate aldolase (DR5P aldolase). One form of DR5P aldolase was induced by...

journal_title：Journal of bacteriology

authors： Hoffee PA

更新日期：1968-02-01 00:00:00

abstract：:IcsA is an outer membrane protein in the autotransporter family that is required for Shigella flexneri pathogenesis. Following its secretion through the Sec translocon, IcsA is incorporated into the outer membrane in a process that depends on YaeT, a component of an outer membrane beta-barrel insertion machinery. We i...

journal_title：Journal of bacteriology

authors： Wagner JK,Heindl JE,Gray AN,Jain S,Goldberg MB

更新日期：2009-02-01 00:00:00

abstract：:A DNA sequence (brpA) which regulates the expression of the genes of the bialaphos biosynthesis pathway (bap) in Streptomyces hygroscopicus was identified and characterized. A newly isolated nonproducing mutant (NP57) had a pleiotropic defect involving at least 6 of the 13 known bap genes; only the step 6 conversion c...

journal_title：Journal of bacteriology

authors： Anzai H,Murakami T,Imai S,Satoh A,Nagaoka K,Thompson CJ

更新日期：1987-08-01 00:00:00

abstract：:The citrate utilization (Cit+) transposon Tn3411 was shown to be flanked by directly repeated sequences (IS3411L and IS3411R) by restriction enzyme analysis and electron microscope observation. Cit- deletion mutants were frequently found to be generated in pBR322::Tn3411 by intramolecular recombination between the two...

journal_title：Journal of bacteriology

authors： Ishiguro N,Sato G

更新日期：1984-11-01 00:00:00

abstract：:Escherichia coli K-12, polAl(-) is a mutant strain whose extracts are deficient in Kornberg deoxyribonucleic acid (DNA) polymerase activity. We have compared the mutant and parental strains on the basis of a number of responses to ultraviolet (UV) and X-irradiation. For both types of radiation, the mutant is more sens...

journal_title：Journal of bacteriology

authors： Paterson MC,Boyle JM,Setlow RB

更新日期：1971-07-01 00:00:00

abstract：:At least six phenotypically distinct classes of mutants of Escherichia coli which require serine or pyridoxine or both can be isolated. Three of the six classes lack 3-phosphoserine-2-oxoglutarate aminotransferase. One of these classes contains WG5, a mutant previously characterized as containing the pdxF5 allele. The...

journal_title：Journal of bacteriology

authors： Dempsey WB,Ito H

更新日期：1970-11-01 00:00:00

abstract：:Outer membrane alterations were characterized in spontaneous mutants of the Erwinia chrysanthemi 3937jRH, which were selected for resistance to bacteriophage phi EC2. All but one of the mutants analyzed were affected in their lipopolysaccharide (LPS) structure, lacking the entire heterogeneous region of apparent high ...

journal_title：Journal of bacteriology

authors： Schoonejans E,Expert D,Toussaint A

更新日期：1987-09-01 00:00:00

abstract：:In Cyanophora paradoxa, the allophycocyanin apoprotein subunits, alpha and beta, are encoded in the cyanelle (plastid) genome. These genes were transferred to the cyanobacterium Synechococcus sp. PCC 7002 on a plasmid replicon. Phycobilisomes isolated from transformed cyanobacteria were found to contain C. paradoxa al...

journal_title：Journal of bacteriology

authors： de Lorimier R,Guglielmi G,Bryant DA,Stevens SE Jr

更新日期：1987-05-01 00:00:00

abstract：:Radiorespirometric and enzymatic analyses reveal that glucose-grown cells of Rhizobium japonicum isolates I-110 and L1-110, both derivatives of R. japonicum strain 3I1b110, possess an active tricarboxylic acid cycle and metabolize glucose by simultaneous operation of the Embden-Meyerhof-Parnas and Entner-Doudoroff pat...

journal_title：Journal of bacteriology

authors： Mulongoy K,Elkan GH

更新日期：1977-07-01 00:00:00

abstract：:The exit of glutamate from Escherichia coli K-12 cells preloaded with the radioactive amino acid and its relation to the reaction of entry were studied. Experiments with cells preloaded to different intracellular concentrations of radioactive glutamate confirmed our earlier conclusion that glutamate exit was a first-o...

journal_title：Journal of bacteriology

authors： Halpern YS,Barash H,Druck K

更新日期：1973-01-01 00:00:00

abstract：:Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunological techniques, we have compared the synthesis of the phoA protein (alkaline phosphatase) and the phoS protein (phosphate-binding protein) in response to the level of phosphate in the medium in different genetic backgrounds containing the kn...

journal_title：Journal of bacteriology

authors： Willsky GR,Malamy MH

更新日期：1976-07-01 00:00:00

abstract：:The tetracycline resistance element of plasmid pBR322 partially complements the potassium transport defect of Escherichia coli K-12 mutants having markedly impaired K+ transport. The plasmid increases K+ transport. The Tn10 element does not result in increased transport, demonstrating that the effect is not general fo...

journal_title：Journal of bacteriology

authors： Dosch DC,Salvacion FF,Epstein W

更新日期：1984-12-01 00:00:00

abstract：:In an Escherichia coli B strain missing ribosomal protein L1, the synthesis rate of L11 is 50% greater than that of other ribosomal proteins. This finding is in agreement with the previous conclusion that L1 regulates synthesis of itself and L11 and indicates that this regulation is important for maintaining the balan...

journal_title：Journal of bacteriology

authors： Jinks-Robertson S,Nomura M

更新日期：1981-03-01 00:00:00

abstract：:In Escherichia coli, repression of the aceBAK operon is mediated by the IclR protein. We used an in vitro oligonucleotide selection technique to determine the consensus recognition sequence for MR. Mutational analysis confirmed the contribution of this sequence to repression in vivo and identified the -35 element of t...

journal_title：Journal of bacteriology

authors： Pan B,Unnikrishnan I,LaPorte DC

更新日期：1996-07-01 00:00:00

abstract：:Xylella fastidiosa is a phytopathogenic bacterium that is responsible for diseases in many economically important crops. Although different strains have been studied, little is known about X. fastidiosa stress responses. One of the better characterized stress responses in bacteria is the heat shock response, which ind...

journal_title：Journal of bacteriology

authors： Koide T,Vêncio RZ,Gomes SL

更新日期：2006-08-01 00:00:00

abstract：:We isolated five new temperature-sensitive alleles of the essential cell division gene ftsZ in Escherichia coli, using P1-mediated, localized mutagenesis. The five resulting single amino acid changes (Gly109-->Ser109 for ftsZ6460, Ala129-->Thr129 for ftsZ972, Val157-->Met157 for ftsZ2066, Pro203-->Leu203 for ftsZ9124,...

journal_title：Journal of bacteriology

authors： Addinall SG,Small E,Whitaker D,Sturrock S,Donachie WD,Khattar MM

更新日期：2005-01-01 00:00:00

abstract：:The central metabolic model for Geobacter sulfurreducens included a single pathway for the biosynthesis of isoleucine that was analogous to that of Escherichia coli, in which the isoleucine precursor 2-oxobutanoate is generated from threonine. 13C labeling studies performed in G. sulfurreducens indicated that this pat...

journal_title：Journal of bacteriology

authors： Risso C,Van Dien SJ,Orloff A,Lovley DR,Coppi MV

更新日期：2008-04-01 00:00:00

abstract：:The aerobic respiratory chain of Escherichia coli can function with either of two different membrane-bound NADH dehydrogenases (NDH-1 and NDH-2) and with either of two ubiquinol oxidases (bd-type and bo-type). The amounts of each of these enzymes present in the E. coli membrane depend on growth conditions in general a...

journal_title：Journal of bacteriology

authors： Calhoun MW,Oden KL,Gennis RB,de Mattos MJ,Neijssel OM

更新日期：1993-05-01 00:00:00

abstract：:The energy source shift-down described in the preceding paper (Molin et al., J. Bacteriol. 131: 7-17, 1977) was used to study the effects of shift-down on protein synthesis. The overall rate of protein synthesis was reduced immediately, and to the same extent, in stringent and relaxed strains. The primary effect of th...

journal_title：Journal of bacteriology

authors： Johnsen K,Molin S,Karlström O,Maaloe O

更新日期：1977-07-01 00:00:00

abstract：:Studies were made on the physical and chemical properties of polysaccharides synthesized by cell-free extracts of Streptococcus mutans, Streptococcus sanguis, and Streptococcus sp. and their susceptibilities to dextranases. Among the polysaccharides examined, insoluble glucans were rather resistant to available dextra...

journal_title：Journal of bacteriology

authors： Ebisu S,Kato K,Kotani S,Misaki A

更新日期：1975-12-01 00:00:00

abstract：:Type III secretion systems (T3SSs) are essential virulence devices for many gram-negative bacteria that are pathogenic for plants, animals, and humans. They serve to translocate virulence effector proteins directly into eukaryotic host cells. T3SSs are composed of a large cytoplasmic bulb and a transmembrane region in...

journal_title：Journal of bacteriology

authors： Veenendaal AK,Sundin C,Blocker AJ

更新日期：2009-01-01 00:00:00

abstract：:Whole cells of Bacillus subtilis transported d-alanine and l-alanine by two different systems. The high-affinity system (K(m) of 1 muM and V(max) of 0.6 to 0.8 nmol/min per mg of protein) was specific for the two stereoisomers of alanine. The low-affinity system (K(m) of 10 muM for l-alanine and 20 muM for d-alanine a...

journal_title：Journal of bacteriology

authors： Clark VL,Young FE

更新日期：1974-12-01 00:00:00