Abstract:
:The energy source shift-down described in the preceding paper (Molin et al., J. Bacteriol. 131: 7-17, 1977) was used to study the effects of shift-down on protein synthesis. The overall rate of protein synthesis was reduced immediately, and to the same extent, in stringent and relaxed strains. The primary effect of the shift was a slowing down of the polypeptide chain growth rate, a finding not previously reported. In stringent strains the normal, preshift rate was reestablished within 2 to 3 min, whereas in relaxed strains the chain growth rate remained low for about 20 min before slowly returning to the normal value, which was reestablished some 50 to 60 min after the shift. Throughout this transition, the stability of messenger ribonucleic acid (mRNA) remained unchanged in both strains. We interpret these findings as evidence of the more rapid reduction of the mRNA pool in the stringent strain after shift-down: we believe that very soon after the shift, the stringent strain reduces its pool of mRNA and with it the number of ribosomes engaged in protein synthesis. In this manner the number of active ribosomes is adjusted to the availability of energy and carbon. The relaxed strain cannot rapidly reduce its mRNA pool, which thus remains large enough to engage a near-preshift number of ribosomes during a prolonged period; as a consequence its ribosomes must work at a reduced rate. The possibility that ppGpp is involved in the control of mRNA production is discussed. After shift-down, the initial part of beta-galactosidase (the auto-alpha fragment) was produced at a higher rate than complete beta-galactosidase in the relaxed strain, as expected when translation is impeded.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Johnsen K,Molin S,Karlström O,Maaloe Odoi
10.1128/JB.131.1.18-29.1977subject
Has Abstractpub_date
1977-07-01 00:00:00pages
18-29issue
1eissn
0021-9193issn
1098-5530journal_volume
131pub_type
杂志文章abstract::Growth rate control of adherent, sessile populations was achieved by the controlled perfusion of membrane-associated bacterial biofilms by the method of Gilbert et al. (P. Gilbert, D. G. Allison, D. J. Evans, P. S. Handley, and M. R. W. Brown, Appl. Environ. Microbiol. 55:1308-1311, 1989). Changes in cell surface hydr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.3.1667-1669.1990
更新日期:1990-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.1.70-77.1985
更新日期:1985-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.174.5.1694-1697.1992
更新日期:1992-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-02-01 00:00:00
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doi:10.1128/JB.88.3.795-801.1964
更新日期:1964-09-01 00:00:00
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doi:10.1128/JB.98.1.62-68.1969
更新日期:1969-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.145.1.479-488.1981
更新日期:1981-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.133.1.85-90.1978
更新日期:1978-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.2.818-821.1984
更新日期:1984-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.169.7.2938-2944.1987
更新日期:1987-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1974-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.525-530.1990
更新日期:1990-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.507-509.1982
更新日期:1982-07-01 00:00:00
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更新日期:2013-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1967-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.23.7446-7455.1997
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abstract::Reich, Paul R. (National Institutes of Health, Bethesda, Md.), Norman L. Somerson, James A. Rose, and Sherman M. Weissman. Genetic relatedness among mycoplasmas as determined by nucleic acid homology. J. Bacteriol. 91:153-160. 1966.-A sensitive membrane filter method to detect nucleic acid homology was used to determi...
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pub_type: 杂志文章
doi:10.1128/JB.91.1.153-160.1966
更新日期:1966-01-01 00:00:00