Purification and characterization of Streptomyces sialidases.

Abstract:

:Some strains of Streptomyces produce sialidases. Two sialidases were purified over 1,000-fold from a culture filtrate of two Streptomyces species. They had the same properties in molecular weight, behavior to ions and other reagents, and substrate specificity. They showed very small differences in kinetic properties, pH optima, and heat stability. These Streptomyces sialidases differed markedly from Clostridium perfringens sialidase in molecular weight, p-chloromercuribenzoate sensitivity, and substrate specificity. Approximate molecular weights of the sialidases from Streptomyces and C. perfringens were 32,000 and 57,000, respectively. p-Chloromercuribenzoate (10(-3) M) caused complete inhibition of C. perfringens sialidase but not of Streptomyces sialidases.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kunimoto S,Aoyagi T,Takeuchi T,Umezawa H

doi

10.1128/JB.119.2.394-400.1974

subject

Has Abstract

pub_date

1974-08-01 00:00:00

pages

394-400

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

119

pub_type

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