Abstract:
:The following results with an in vitro replication system utilizing a plasmid RK2 DNA-membrane complex indicate that the essential trfA-encoded replication protein of RK2 is present and active in the complex. (i) A complex extracted from a conditional replication mutant of RK2, which contains a temperature-sensitive mutation in trfA, displayed extensive DNA synthesis at the permissive temperature but little activity at the restrictive temperature. A control wild-type RK2 complex showed no inhibition of DNA synthesis at the restrictive temperature. (ii) Analysis of plasmid-encoded proteins revealed that the trfA-specified replication protein and other proteins which may be involved in the replication and maintenance of RK2 are located physically in the complex. Semiconservative plasmid DNA replication by the DNA-membrane complex was indicated by density shift experiments; DNA synthesized in the presence of a heavy-density precursor banded primarily in a heavier-density area of a neutral CsCl density gradient and consisted mostly of heavy- and light-density single-stranded DNA as determined by alkaline CsCl density gradient centrifugation. Plasmid RK2 DNA replication by the DNA-membrane complex appears to be coupled to transcription and translation as indicated by the following results: the inhibitory effects of chloramphenicol on both DNA and protein synthesis by the complex; the stimulation of replication by components normally required for protein synthesis (tRNA and all the common amino acids); the synthesis of RNA and protein by the complex; and the synthesis of specific RK2-encoded proteins.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kornacki JA,Firshein Wdoi
10.1128/jb.167.1.319-326.1986subject
Has Abstractpub_date
1986-07-01 00:00:00pages
319-26issue
1eissn
0021-9193issn
1098-5530journal_volume
167pub_type
杂志文章abstract::Glucose-1-(14)C and acetylglucosamine-1-(14)C were added singly and together with equal amounts of the unlabeled reciprocal to Brain Heart Infusion and used for the culture of Streptococcus pyogenes. The labeling pattern of the rhamnose, glucosamine, and muramic acid in the cell wall supported an intermediary role for...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.4.963-965.1967
更新日期:1967-10-01 00:00:00
abstract::We describe here a new mutant of Pseudomonas aeruginosa PAO, strain D10C (genotype plcB), which produces phospholipase C and alkaline phosphatase constitutively. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the extracellular proteins produced by this mutant in high- and low-Pi media revealed that the m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.3.1221-1226.1982
更新日期:1982-06-01 00:00:00
abstract::The CpxA-CpxR two-component signal transduction pathway of Escherichia coli was studied in a mutant (pss-93) lacking phosphatidylethanolamine (PE). Several properties of this mutant are comparable to phenotypes of cpxA point mutants, indicating that this two-component pathway is activated in PE-deficient cells. In con...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.4.1029-1034.1997
更新日期:1997-02-01 00:00:00
abstract::Swarmer cell differentiation is a complex process involving the activity of many gene products. In this report, we characterized the genetic locus of Tn5 insertion in each of 12 mutants defective in swarmer cell elongation. The mutations fell into four categories affecting either flagellar biosynthesis or energetics, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.3.823-828.1995
更新日期:1995-02-01 00:00:00
abstract::Acetate kinase (ACK) converts acetyl phosphate to acetate along with the generation of ATP in the pathway for mixed-acid fermentation in Lactococcus lactis. The reverse reaction yields acetyl phosphate for assimilation purposes. Remarkably, L. lactis has two ACK isozymes, and the corresponding genes are present in an ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01277-13
更新日期:2014-04-01 00:00:00
abstract::A bacterium was isolated by elective culture with p-hydroxybenzoate as substrate and nitrate as electron acceptor. It grew either aerobically or anaerobically, by nitrate respiration, on a range of aromatic compounds. The organism was identified as a pseudomonad and was given the trivial name Pseudomonas PN-1. Benzoat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.2.430-437.1970
更新日期:1970-05-01 00:00:00
abstract::Huston, Charles K. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), and Phillip W. Albro. Lipids of Sarcina lutea. I. Fatty acid composition of the extractable lipids. J. Bacteriol. 88:425-432. 1964.-The extractable lipids of Sarcina lutea were separated into several fractions by a combination of col...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.2.425-432.1964
更新日期:1964-08-01 00:00:00
abstract::Transcriptome analysis of a Bacillus subtilis rok strain that showed reduced complex colony structure formation revealed significant downregulation of the yuaB gene. Overexpression of yuaB restored structure formation in the rok strain. We show that transcription of yuaB is indirectly regulated by Rok, independently f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01170-10
更新日期:2011-02-01 00:00:00
abstract::Thorne, Curtis B. (Fort Detrick, Frederick, Md.). Transduction in Bacillus subtilis. J. Bacteriol. 83:106-111. 1962.-A bacteriophage, SP-10, isolated from soil carries out general transduction in Bacillus subtilis. Phage propagated on a streptomycin-resistant mutant of the wild-type strain W-23 was capable of transduc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.1.106-111.1962
更新日期:1962-01-01 00:00:00
abstract::Listeria monocytogenes is able to efficiently utilize glycerol as a carbon source. In a defined minimal medium, the growth rate (during balanced growth) in the presence of glycerol is similar to that in the presence of glucose or cellobiose. Comparative transcriptome analyses of L. monocytogenes showed high-level tran...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00259-08
更新日期:2008-08-01 00:00:00
abstract::An examination of the methanofurans isolated from a wide range of methanogenic bacteria and from Archaeoglobus fulgidus has revealed at least five chromatographically distinct methanofurans. Bacteria from each major genus of methanogenic bacteria have been found to contain a chemically different methanofuran. The natu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4594-4597.1988
更新日期:1988-10-01 00:00:00
abstract::Lipopolysaccharide is a major glycolipid component in the outer leaflet of the outer membrane (OM), a peculiar permeability barrier of Gram-negative bacteria that prevents many toxic compounds from entering the cell. Lipopolysaccharide transport (Lpt) across the periplasmic space and its assembly at the Escherichia co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02057-12
更新日期:2013-03-01 00:00:00
abstract::Helicobacter pylori is a human gastric pathogen infecting almost half of the world population. Herein, we present an updated version of the metabolic reconstruction of H. pylori strain 26695 based on the revised genome annotation and new experimental data. This reconstruction, iIT341 GSM/GPR, represents a detailed rev...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.16.5818-5830.2005
更新日期:2005-08-01 00:00:00
abstract::The extent and nature of DNA polymorphism in the mutS-rpoS region of the Escherichia coli genome were assessed in 21 strains of enteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) and in 6 strains originally isolated from natural populations. The intervening region between mutS and rpoS was amplified ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.19.5381-5390.2000
更新日期:2000-10-01 00:00:00
abstract::A number of laboratories are now engaged in the genetic analysis of Candida albicans. This diploid yeast, the major fungal pathogen of humans, is imperfect. Parasexual techniques have been devised for complementation and recombination analysis in this organism. This paper attempts to address the question of the extent...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.2.498-506.1983
更新日期:1983-11-01 00:00:00
abstract::Auxotrophic mutants of the filamentous cyanobacterium Anabaena variabilis were isolated by a method in which, after mutagenesis and before penicllin enrichment, mutant and wild-type cells were separated by cavitation. Auxotrophs were identified by their inability to grow on minimal medium, and they were partially char...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.3.1556-1562.1977
更新日期:1977-03-01 00:00:00
abstract::Low-copy-number plasmids generally encode a partitioning system to ensure proper segregation after replication. Little is known about partitioning of linear plasmids in Streptomyces. SLP2 is a 50-kb low-copy-number linear plasmid in Streptomyces lividans, which contains a typical parAB partitioning operon. In S. livid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01192-09
更新日期:2010-01-01 00:00:00
abstract::Certain oral treponemes express a highly proteolytic phenotype and have been associated with periodontal diseases. The periodontal pathogen Treponema denticola produces dentilisin, a serine protease of the subtilisin family. The two-gene operon prcA-prtP is required for expression of active dentilisin (PrtP), a putati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.23.6860-6869.2003
更新日期:2003-12-01 00:00:00
abstract::Pseudomonas aeruginosa hemolytic phospholipase C (PlcH) degrades phosphatidylcholine (PC), an abundant lipid in cell membranes and lung surfactant. A ΔplcHR mutant, known to be defective in virulence in animal models, was less able to colonize epithelial cell monolayers and was defective in biofilm formation on plasti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02169-12
更新日期:2013-07-01 00:00:00
abstract::The genetic organization of the polypeptides required for the biosynthesis of the F41 adhesin of enterotoxigenic Escherichia coli strains was investigated. Maxicell analysis demonstrated that a recombinant plasmid which mediated mannose-resistant hemagglutination and F41 antigen production encoded four polypeptides of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4890-4896.1988
更新日期:1988-10-01 00:00:00
abstract::Fowler, Audree V. (University of California, Los Angeles), and Irving Zabin. Effects of dimethylsulfoxide on the lactose operon in Escherichia coli. J. Bacteriol. 92:353-357. 1966.-Dimethylsulfoxide (DMSO) at a concentration of 5% (v/v) in the culture medium inhibits the growth of Escherichia coli to only a slight ext...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.2.353-357.1966
更新日期:1966-08-01 00:00:00
abstract::The gal3 mutation of Saccharomyces, which is associated with an impairment in the utilization of galactose, has been shown to be pleiotropic, causing similar impairments in the utilization of melibiose and maltose. Milibiose utilization and alpha-galactosidase production are directly controlled by the galactose regula...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.33-41.1976
更新日期:1976-01-01 00:00:00
abstract::A genetic element integrating site specifically into a prokaryotic gene usually carries a copy of the 3' portion of that gene that restores the active gene even as the original is disrupted. A cryptic element in Mesorhizobium loti instead carries a copy of the 5' end of the tRNA gene into which it integrated. This has...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.3.859-860.2002
更新日期:2002-02-01 00:00:00
abstract::Faine, S. (University of Sydney, Sydney, Australia), and J. van der Hoeden. Virulence-linked colonial and morphological variation in Leptospira. J. Bacteriol. 88:1493-1496. 1964.-Large-colony typical hooked Leptospira icterohaemorrhagiae was virulent for hamsters and guinea pigs. On cultivation, it was gradually repla...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.5.1493-1496.1964
更新日期:1964-11-01 00:00:00
abstract::In previous work, we characterized the bases in an iteron of plasmid R6K that are important for the binding of pi protein monomers and dimers. Here we investigate the following six amino acids of pi, encoded by pir, hypothesized to be important for DNA contact: Ser71, Try74, Gly131, Gly211, Arg225, and Arg254. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00109-07
更新日期:2007-07-01 00:00:00
abstract::Neisseria gonorrhoeae releases peptidoglycan fragments during growth. The majority of the fragments released are peptidoglycan monomers, molecules known to increase pathogenesis through the induction of proinflammatory cytokines and responsible for the killing of ciliated epithelial cells. In other gram-negative bacte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01194-07
更新日期:2008-06-01 00:00:00
abstract::Mutants of Escherichia coli K-12 unable to excise pyrimidine dimers from their deoxyribonucleic acid (DNA) because of a uvr mutation show a higher survival when plated on a minimal salts medium after exposure to ultraviolet radiation than when plated on a complex medium such as nutrient agar containing yeast extract. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.2.404-410.1970
更新日期:1970-05-01 00:00:00
abstract:UNLABELLED:Many bacteria produce secreted iron chelators called siderophores, which can be shared among cells with specific siderophore uptake systems regardless of whether the cell produces siderophores. Sharing secreted products allows freeloading, where individuals use resources without bearing the cost of productio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02596-14
更新日期:2015-07-01 00:00:00
abstract::A characteristic feature of biofilm formation is the production of a protective extracellular polymeric matrix. In the gram-positive bacterium Bacillus subtilis, the biofilm matrix is synthesized by the products of the epsABCDEFGHIJKLMNO operon (hereafter called the eps operon) and yqxM-sipW-tasA loci. Transcription f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00618-09
更新日期:2009-11-01 00:00:00
abstract::Fertility factor F confers bacterial conjugation, a process which involves at least 20 tra genes. Resistance plasmids such as R100, R6-5, and R1 have homology with F in the tra region. Conjugal transfer of these plasmids is, however, repressed, while transfer of F is constitutive. Repression of R transfer is due to th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.2.619-623.1987
更新日期:1987-02-01 00:00:00