Repressor gene finO in plasmids R100 and F: constitutive transfer of plasmid F is caused by insertion of IS3 into F finO.


:Fertility factor F confers bacterial conjugation, a process which involves at least 20 tra genes. Resistance plasmids such as R100, R6-5, and R1 have homology with F in the tra region. Conjugal transfer of these plasmids is, however, repressed, while transfer of F is constitutive. Repression of R transfer is due to the existence of the two genes, called finO and finP; constitutive transfer of F is believed to be due to a lack of finO in F. In this paper, we report the identification and DNA sequence of the finO gene of R100, encoding a protein of 21,265 daltons. We show that F does actually encode finO, but the gene has been inactivated by insertion of IS3. Lederberg and Tatum (Nature [London] 158:558, 1946), who discovered sexuality in bacteria, may have had an Escherichia coli K-12 strain harboring such an finO F factor, which facilitated the generation of recombinant progeny useful for genetic analysis of bacteria and established the foundation for molecular genetics.


J Bacteriol


Journal of bacteriology


Yoshioka Y,Ohtsubo H,Ohtsubo E




Has Abstract


1987-02-01 00:00:00












  • AerR, a second aerobic repressor of photosynthesis gene expression in Rhodobacter capsulatus.

    abstract::Open reading frame orf192, which is located immediately upstream of the aerobic repressor gene crtJ, was genetically and biochemically demonstrated to code for a second aerobic repressor (AerR) of photosynthesis gene expression in Rhodobacter capsulatus. Promoter-mapping studies indicate that crtJ has its own promoter...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Dong C,Elsen S,Swem LR,Bauer CE

    更新日期:2002-05-01 00:00:00

  • Functional Overlap of hetP and hetZ in Regulation of Heterocyst Differentiation in Anabaena sp. Strain PCC 7120.

    abstract::HetR plays a key role in regulation of heterocyst differentiation and patterning in Anabaena It directly regulates genes involved in heterocyst differentiation (such as hetP and hetZ), genes involved in pattern formation (patA), and many others. In this study, we investigated the functional relationship of hetP and he...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zhang H,Wang S,Wang Y,Xu X

    更新日期:2018-04-09 00:00:00

  • Effect of D-amino acids on structure and synthesis of peptidoglycan in Escherichia coli.

    abstract::Growth of Escherichia coli in the presence of certain D-amino acids, such as D-methionine, results in the incorporation of the D-amino acid into macromolecular peptidoglycan and can be lethal at high concentrations. Previous studies suggested that incorporation was independent of the normal biosynthetic pathway. An en...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Caparrós M,Pisabarro AG,de Pedro MA

    更新日期:1992-09-01 00:00:00

  • Temperature-sensitive sporulation caused by a mutation in the Bacillus subtilis secY gene.

    abstract::A thermosensitive sporulation mutant of Bacillus subtilis containing a mutation in the secY gene was isolated and characterized. No asymmetric septum specific to the sporulation was detected by electron microscopy at the nonpermissive temperature, indicating that the block occurred at a very early stage of sporulation...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yoshikawa H,Jeong SM,Hirata A,Kawamura F,Doi RH,Takahashi H

    更新日期:1993-06-01 00:00:00

  • Repression of 3-deoxy-D-arabinoheptulosonic acid-7-phosphate synthetase (trp) and enzymes of the tryptophan pathway in Escherichia coli K-12.

    abstract::Mutant strains of Escherichia coli K-12 have been isolated in which the synthesis of 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase (trp) is partially constitutive. The mutation causing derepression is closely linked to aroH [the structural gene for DAHP synthetase (trp)] and occurs in a locus design...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Camakaris J,Pittard J

    更新日期:1971-08-01 00:00:00

  • Proline-dependent regulation of Clostridium difficile Stickland metabolism.

    abstract::Clostridium difficile, a proteolytic Gram-positive anaerobe, has emerged as a significant nosocomial pathogen. Stickland fermentation reactions are thought to be important for growth of C. difficile and appear to influence toxin production. In Stickland reactions, pairs of amino acids donate and accept electrons, gene...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bouillaut L,Self WT,Sonenshein AL

    更新日期:2013-02-01 00:00:00

  • Immunochemistry of the pneumococcal types II, V, and VI. III. Tests with derivatives of the specific polysaccharides of types II and VI.

    abstract::Rebers, P. A. (Rutgers, The State University, New Brunswick, N. J.), E. Hurwitz, M. Heidelberger, and S. Estrada-Parra. Immunochemistry of the pneumococcal types II, V, and VI. III. Tests with derivatives of the specific polysaccharides of types II and VI. J. Bacteriol. 83:335-342. 1962.-The type-specific pneumococcal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1962-02-01 00:00:00

  • The VirA protein of Agrobacterium tumefaciens is autophosphorylated and is essential for vir gene regulation.

    abstract::The virA and virG gene products are required for the regulation of the vir regulon on the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens. VirA is a membrane-associated protein which is homologous to the sensor molecules of other two-component regulatory systems. We overproduced truncated VirA proteins in Esc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jin S,Roitsch T,Ankenbauer RG,Gordon MP,Nester EW

    更新日期:1990-02-01 00:00:00

  • Draft genome sequence of Paenisporosarcina sp. strain TG-20, a psychrophilic bacterium isolated from the basal ice of Taylor Glacier.

    abstract::We report the draft genome sequence of Paenisporosarcina sp. strain TG-20, which is 4.12 Mb in size and consists of 4,071 protein-coding genes and 76 RNA genes. The genome sequence of Paenisporosarcina sp. TG-20 may provide useful information about molecular adaptations that enhance survival in icy subsurface environm...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lee JH,Koh HY,Lee SG,Doyle S,Christner BC,Kim HJ

    更新日期:2012-12-01 00:00:00

  • Spontaneous erythromycin resistance mutation in a 23S rRNA gene, rrlA, of the extreme thermophile Thermus thermophilus IB-21.

    abstract::Spontaneous, erythromycin-resistant mutants of Thermus thermophilus IB-21 were isolated and found to carry the mutation A2058G in one of two 23S rRNA operons. The heterozygosity of these mutants indicates that A2058G confers a dominant or codominant phenotype in this organism. This mutation provides a valuable tool fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gregory ST,Cate JH,Dahlberg AE

    更新日期:2001-07-01 00:00:00

  • Cytochrome bd oxidase, oxidative stress, and dioxygen tolerance of the strictly anaerobic bacterium Moorella thermoacetica.

    abstract::The gram-positive, thermophilic, acetogenic bacterium Moorella thermoacetica can reduce CO2 to acetate via the Wood-Ljungdahl (acetyl coenzyme A synthesis) pathway. This report demonstrates that, despite its classification as a strict anaerobe, M. thermoacetica contains a membrane-bound cytochrome bd oxidase that can ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Das A,Silaghi-Dumitrescu R,Ljungdahl LG,Kurtz DM Jr

    更新日期:2005-03-01 00:00:00

  • Identification and characterization of noncoding small RNAs in Streptococcus pneumoniae serotype 2 strain D39.

    abstract::We report a search for small RNAs (sRNAs) in the low-GC, gram-positive human pathogen Streptococcus pneumoniae. Based on bioinformatic analyses by Livny et al. (J. Livny, A. Brencic, S. Lory, and M. K. Waldor, Nucleic Acids Res. 34:3484-3493, 2006), we tested 40 candidates by Northern blotting and confirmed the expres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tsui HC,Mukherjee D,Ray VA,Sham LT,Feig AL,Winkler ME

    更新日期:2010-01-01 00:00:00

  • Abortive phage resistance mechanism AbiZ speeds the lysis clock to cause premature lysis of phage-infected Lactococcus lactis.

    abstract::The conjugative plasmid pTR2030 has been used extensively to confer phage resistance in commercial Lactococcus starter cultures. The plasmid harbors a 16-kb region, flanked by insertion sequence (IS) elements, that encodes the restriction/modification system LlaI and carries an abortive infection gene, abiA. The AbiA ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Durmaz E,Klaenhammer TR

    更新日期:2007-02-01 00:00:00

  • Growth of mycobacteria on carbon monoxide and methanol.

    abstract::Several mycobacterial strains, such as Mycobacterium flavescens, Mycobacterium gastri, Mycobacterium neoaurum, Mycobacterium parafortuitum, Mycobacterium peregrinum, Mycobacterium phlei, Mycobacterium smegmatis, Mycobacterium tuberculosis, and Mycobacterium vaccae, were found to grow on carbon monoxide (CO) as the sol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Park SW,Hwang EH,Park H,Kim JA,Heo J,Lee KH,Song T,Kim E,Ro YT,Kim SW,Kim YM

    更新日期:2003-01-01 00:00:00

  • Disulfide-mediated interactions of the chlamydial major outer membrane protein: role in the differentiation of chlamydiae?

    abstract::The effects of exogenous reducing agents on a number of biological properties of purified Chlamydia trachomatis LGV-434 and Chlamydia psittaci meningopneumonitis elementary bodies (EBs) have been examined in an attempt to identify in vitro correlates of early events in the differentiation of the infectious EB to the r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hackstadt T,Todd WJ,Caldwell HD

    更新日期:1985-01-01 00:00:00

  • Uranyl nitrate inhibition of transport systems in Saccharomyces cerevisiae.

    abstract::Uranyl nitrate inhibited the transport of several amino acids, a vitamin, and a disaccharide into yeast. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Maxwell WA,Metzler R,Spoerl E

    更新日期:1971-03-01 00:00:00

  • Choline fermentation by Desulfovibrio desulfuricans.

    abstract::Baker, F. D. (Western Reserve University, Cleveland, Ohio), H. R. Papiska, and L. Leon Campbell. Choline fermentation by Desulfovibrio desulfuricans. J. Bacteriol. 84:973-978. 1962-Hayward and Stadtman pointed out that the organism they described as Vibrio cholinicus is closely related to Desulfovibrio desulfuricans. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1962-11-01 00:00:00

  • Biochemical and serological evidence for an RNase E-like activity in halophilic Archaea.

    abstract::Endoribonuclease RNase E appears to control the rate-limiting step that mediates the degradation of many mRNA species in bacteria. In this work, an RNase E-like activity in Archaea is described. An endoribonucleolytic activity from the extreme halophile Haloarcula marismortui showed the same RNA substrate specificity ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Franzetti B,Sohlberg B,Zaccai G,von Gabain A

    更新日期:1997-02-01 00:00:00

  • Antibacterial nitroacridine, Nitroakridin 3582: binding to nucleic acids in vitro and effects on selected cell-free model systems of macromolecular biosynthesis.

    abstract::Nitroakridin 3582 (NA) formed complexes with native deoxyribonucleic acid (DNA) and with transfer ribonucleic acid (tRNA) species from Escherichia coli. Spectrophotometric titrations of NA with these nucleic acids produced numerical results from which nonlinear adsorption isotherms were derived. These curves indicated...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wolfe AD,Cook TM,Hahn FE

    更新日期:1971-12-01 00:00:00

  • Acid- and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis.

    abstract::Proteins induced by acid or base, during long-term aerobic or anaerobic growth in complex medium, were identified in Escherichia coli. Two-dimensional gel electrophoresis revealed pH-dependent induction of 18 proteins, nine of which were identified by N-terminal sequencing. At pH 9, tryptophan deaminase (TnaA) was ind...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Blankenhorn D,Phillips J,Slonczewski JL

    更新日期:1999-04-01 00:00:00

  • Cell division in Escherichia coli: role of FtsL domains in septal localization, function, and oligomerization.

    abstract::In Escherichia coli, nine essential cell division proteins are known to localize to the division septum. FtsL is a 13-kDa bitopic membrane protein with a short cytoplasmic N-terminal domain, a membrane-spanning segment, and a periplasmic domain that has a repeated heptad motif characteristic of leucine zippers. Here, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ghigo JM,Beckwith J

    更新日期:2000-01-01 00:00:00

  • Molecular cloning of the protocatechuate 4,5-dioxygenase genes of Pseudomonas paucimobilis.

    abstract::We determined the nucleotide sequence of a 1.9-kilobase fragment of Pseudomonas paucimobilis SYK6 chromosomal DNA that included genes encoding protocatechuate 4,5-dioxygenase, the enzyme responsible for the aromatic ring fission of protocatechuate. Two open reading frames of 417 and 906 base pairs were found that had ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Noda Y,Nishikawa S,Shiozuka K,Kadokura H,Nakajima H,Yoda K,Katayama Y,Morohoshi N,Haraguchi T,Yamasaki M

    更新日期:1990-05-01 00:00:00

  • Purification and biochemical characterization of tellurite-reducing activities from Thermus thermophilus HB8.

    abstract::Cell-free extracts of Thermus thermophilus HB8 catalyze the in vitro, NADH-dependent reduction of potassium tellurite (K2TeO3). Three different protein fractions with tellurite-reducing activities were identified. Two exhibited high molecular weight and were composed of at least two different polypeptides. The protein...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chiong M,González E,Barra R,Vásquez C

    更新日期:1988-07-01 00:00:00

  • An antimicrobial peptide is produced by extracellular processing of a protein from Propionibacterium jensenii.

    abstract::A protease-activated antimicrobial peptide (PAMP) and its inactive precursor were purified from the culture supernatant of Propionibacterium jensenii LMG 3032 and characterized at the molecular level. PAMP is a 64-amino-acid cationic peptide of 6,383 Da with physicochemical features similar to those of bacteriocins fr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Faye T,Brede DA,Langsrud T,Nes IF,Holo H

    更新日期:2002-07-01 00:00:00

  • Transcription of the Escherichia coli fumarate reductase genes (frdABCD) and their coordinate regulation by oxygen, nitrate, and fumarate.

    abstract::The fumarate reductase enzyme complex allows Escherichia coli to grow anaerobically with fumarate as a terminal electron acceptor for oxidative phosphorylation when the preferred compounds oxygen and nitrate are not available. We used the pKO promoter test vectors to identify a single promoter for the frdABCD genes wh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jones HM,Gunsalus RP

    更新日期:1985-12-01 00:00:00

  • Exopolysaccharides produced by Streptococcus mutans glucosyltransferases modulate the establishment of microcolonies within multispecies biofilms.

    abstract::Streptococcus mutans is a key contributor to the formation of the extracellular polysaccharide (EPS) matrix in dental biofilms. The exopolysaccharides, which are mostly glucans synthesized by streptococcal glucosyltransferases (Gtfs), provide binding sites that promote accumulation of microorganisms on the tooth surfa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Koo H,Xiao J,Klein MI,Jeon JG

    更新日期:2010-06-01 00:00:00

  • Phosphonate utilization by bacteria.

    abstract::Bacteria able to use at least one of 13 ionic alkylphosphonates of O-alkyl or O,O-dialkyl alkylphosphonates as phosphorus sources were isolated from sewage and soil. Four of these isolates used 2-aminoethylphosphonic acid (AEP) as a sole carbon, nitrogen, and phosphorus source. None of the other phosphonates served as...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Cook AM,Daughton CG,Alexander M

    更新日期:1978-01-01 00:00:00

  • The phylogeny of marine and freshwater caulobacters reflects their habitat.

    abstract::Caulobacter is a distinctive genus of prosthecate bacteria. Because caulobacters adhere to surfaces and are found in diverse locales, their role in oligotrophic environments and bacterial biofilm communities is of interest. The phylogenetic relationships of a group of marine and freshwater caulobacters were examined i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Stahl DA,Key R,Flesher B,Smit J

    更新日期:1992-04-01 00:00:00

  • The Saccharomyces cerevisiae MEC1 gene, which encodes a homolog of the human ATM gene product, is required for G1 arrest following radiation treatment.

    abstract::The Saccharomyces cerevisiae gene MEC1 represents a structural homolog of the human gene ATM mutated in ataxia telangiectasia patients. Like human ataxia telangiectasia cell lines, mec1 mutants are defective in G2 and S-phase cell cycle checkpoints in response to radiation treatment. Here we show an additional defect ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Siede W,Allen JB,Elledge SJ,Friedberg EC

    更新日期:1996-10-01 00:00:00

  • Pyrimidine biosynthesis in Lactobacillus leichmannii.

    abstract::Tracer studies of pyrimidine biosynthesis in Lactobacillus leichmannii (ATCC 7830) indicated that, while aspartate is utilized in the usual manner, the guanido carbon of arginine, rather than carbon dioxide, is utilized as a pyrimidine precursor. The guanido carbon of arginine also contributes, to some extent, to the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hutson JY,Downing M

    更新日期:1968-10-01 00:00:00