Repressor gene finO in plasmids R100 and F: constitutive transfer of plasmid F is caused by insertion of IS3 into F finO.


:Fertility factor F confers bacterial conjugation, a process which involves at least 20 tra genes. Resistance plasmids such as R100, R6-5, and R1 have homology with F in the tra region. Conjugal transfer of these plasmids is, however, repressed, while transfer of F is constitutive. Repression of R transfer is due to the existence of the two genes, called finO and finP; constitutive transfer of F is believed to be due to a lack of finO in F. In this paper, we report the identification and DNA sequence of the finO gene of R100, encoding a protein of 21,265 daltons. We show that F does actually encode finO, but the gene has been inactivated by insertion of IS3. Lederberg and Tatum (Nature [London] 158:558, 1946), who discovered sexuality in bacteria, may have had an Escherichia coli K-12 strain harboring such an finO F factor, which facilitated the generation of recombinant progeny useful for genetic analysis of bacteria and established the foundation for molecular genetics.


J Bacteriol


Journal of bacteriology


Yoshioka Y,Ohtsubo H,Ohtsubo E




Has Abstract


1987-02-01 00:00:00












  • Regulatory elements of the raffinose operon: nucleotide sequences of operator and repressor genes.

    abstract::The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor (rafR gene) to raf operator (rafO) DNA. Both rafR and rafO have been sequenced. The 1,011-base-pair rafR gene encodes a 336-amino-acid polypeptide containing an N-terminal helix-turn-helix motif. rafO, as defined by in vivo ti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Aslanidis C,Schmitt R

    更新日期:1990-04-01 00:00:00

  • Identification and functional verification of archaeal-type phosphoenolpyruvate carboxylase, a missing link in archaeal central carbohydrate metabolism.

    abstract::Despite the fact that phosphoenolpyruvate carboxylase (PEPC) activity has been measured and in some cases even purified from some Archaea, the gene responsible for this activity has not been elucidated. Using sensitive sequence comparison methods, we detected a highly conserved, uncharacterized archaeal gene family th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ettema TJ,Makarova KS,Jellema GL,Gierman HJ,Koonin EV,Huynen MA,de Vos WM,van der Oost J

    更新日期:2004-11-01 00:00:00

  • Isolation and characterization of a gene from Streptomyces sp. strain C5 that confers the ability to convert daunomycin to doxorubicin on Streptomyces lividans TK24.

    abstract::DNA sequence analysis of a region of the Streptomyces sp. strain C5 daunomycin biosynthesis gene cluster, located between the daunomycin polyketide biosynthesis gene cluster and a dnrI (transcriptional activator) homolog, revealed the presence of a gene encoding a P-450-like enzyme with a deduced Mr of 46,096. Express...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Dickens ML,Strohl WR

    更新日期:1996-06-01 00:00:00

  • Mutational analysis of Agrobacterium tumefaciens virD2: tyrosine 29 is essential for endonuclease activity.

    abstract::Agrobacterium tumefaciens VirD2 polypeptide, in the presence of VirD1, catalyzes a site- and strand-specific nicking reaction at the T-DNA border sequences. VirD2 is found tightly attached to the 5' end of the nicked DNA. The protein-DNA complex is presumably formed via a tyrosine residue of VirD2 (F. Durrenberger, A....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vogel AM,Das A

    更新日期:1992-01-01 00:00:00

  • Identification of active site residues by site-directed mutagenesis of delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B.

    abstract::In order to assess the roles of specific amino acid residues in the delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B during catalysis, we replaced aspartic acid 40 with asparagine (D40N) and tyrosine 16 with phenylalanine (Y16F) in the enzyme by site-directed mutagenesis. Both purified mutant enzymes ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kim SW,Choi KY

    更新日期:1995-05-01 00:00:00

  • Physical and genetic map of the Serpulina hyodysenteriae B78T chromosome.

    abstract::A combined physical and genetic map of the Serpulina hyodysenteriae B78T genome was constructed by using pulsed-field gel electrophoresis and DNA blot hybridizations. The S. hyodysenteriae genome is a single circular chromosome about 3.2 Mb in size. The physical map of the chromosome was constructed with the restricti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zuerner RL,Stanton TB

    更新日期:1994-02-01 00:00:00

  • Glutamate transport in Escherichia coli K-12: nonidentity of carriers mediating entry and exit.

    abstract::The exit of glutamate from Escherichia coli K-12 cells preloaded with the radioactive amino acid and its relation to the reaction of entry were studied. Experiments with cells preloaded to different intracellular concentrations of radioactive glutamate confirmed our earlier conclusion that glutamate exit was a first-o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Halpern YS,Barash H,Druck K

    更新日期:1973-01-01 00:00:00

  • Complete nucleotide sequence of macrolide-lincosamide-streptogramin B-resistance transposon Tn917 in Streptococcus faecalis.

    abstract::Streptococcus faecalis transposon Tn917 was cloned in Escherichia coli on plasmid vector pBR325. The erythromycin resistance determinant of Tn917 was not expressed in the E. coli background. The nucleotide sequence of Tn917 was determined and found to be 5,257 base pairs in length. Six open reading frames (ORFs) were ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Shaw JH,Clewell DB

    更新日期:1985-11-01 00:00:00

  • YycH and YycI interact to regulate the essential YycFG two-component system in Bacillus subtilis.

    abstract::The YycFG two-component system is the only signal transduction system in Bacillus subtilis known to be essential for cell viability. This system is highly conserved in low-G+C gram-positive bacteria, regulating important processes such as cell wall homeostasis, cell membrane integrity, and cell division. Four other ge...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Szurmant H,Mohan MA,Imus PM,Hoch JA

    更新日期:2007-04-01 00:00:00

  • Deoxyribonucleic acid synthesis in synchronously growing Mycoplasma gallisepticum.

    abstract::Early log-phase cells of Mycoplasma gallisepticum A5969 were synchronized by holding in Eagle minimal essential medium (MEM) for 2 h. When transferred out of MEM into tryptose medium, the cells exhibited synchronous growth. Deoxyribonucleic acid (DNA) synthesis proceeded continuously during this growth but stopped dur...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Quinlan DC,Maniloff J

    更新日期:1973-07-01 00:00:00

  • Photosynthetic sulfide oxidation by Chloroflexus aurantiacus, a filamentous, photosynthetic, gliding bacterium.

    abstract::Chloroflexus, a newly described genus of filamentous, photosynthetic, gliding bacteria, oxidizes sulfide anaerobically under photoautotrophic or photoheterotrophic growh conditions and deposits elemental sulfur outside the cell. The formation of sulfur granules outside the cell supports the idea that this organism is ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Madigan MT,Brock TD

    更新日期:1975-05-01 00:00:00

  • Core and panmetabolism in Escherichia coli.

    abstract::Escherichia coli exhibits a wide range of lifestyles encompassing commensalism and various pathogenic behaviors which its highly dynamic genome contributes to develop. How environmental and host factors shape the genetic structure of E. coli strains remains, however, largely unknown. Following a previous study of E. c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vieira G,Sabarly V,Bourguignon PY,Durot M,Le Fèvre F,Mornico D,Vallenet D,Bouvet O,Denamur E,Schachter V,Médigue C

    更新日期:2011-03-01 00:00:00

  • Purification and characterization of catabolic mannopine cyclase encoded by the Agrobacterium tumefaciens Ti plasmid pTi15955.

    abstract::Catabolic mannopine (MOP) cyclase encoded by certain Agrobacterium Ti and Ri plasmids lactonizes MOP to agropine (AGR). The enzyme, purified to homogeneity from a recombinant clone, has a molecular mass of 45 kDa as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and size exclusion chromatography...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hong SB,Farrand SK

    更新日期:1996-04-01 00:00:00

  • The ABC-type multidrug resistance transporter LmrCD is responsible for an extrusion-based mechanism of bile acid resistance in Lactococcus lactis.

    abstract::Upon prolonged exposure to cholate and other toxic compounds, Lactococcus lactis develops a multidrug resistance phenotype that has been attributed to an elevated expression of the heterodimeric ABC-type multidrug transporter LmrCD. To investigate the molecular basis of bile acid resistance in L. lactis and to evaluat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zaidi AH,Bakkes PJ,Lubelski J,Agustiandari H,Kuipers OP,Driessen AJ

    更新日期:2008-11-01 00:00:00

  • L-cysteine biosynthesis in Bacillus subtilis: identification, sequencing, and functional characterization of the gene coding for phosphoadenylylsulfate sulfotransferase.

    abstract::Random Tn917 mutagenesis of Bacillus subtilis followed by selection of lipoic acid auxotrophs led to the isolation of the cysH gene. The gene was sequenced and found to encode a phosphoadenylylsulfate sulfotransferase with a molecular mass of 27 kDa. Expression of lacZ fused to the cysH promoter was repressed by cyste...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mansilla MC,de Mendoza D

    更新日期:1997-02-01 00:00:00

  • A cryptic miniplasmid from the hyperthermophilic bacterium Thermotoga sp. strain RQ7.

    abstract::An 846-bp cryptic plasmid has been discovered in the hyperthermophilic bacterium Thermotoga sp. strain RQ7. This is the first plasmid described for an organism from this ancient bacterial lineage and the smallest plasmid described to date for any organism. Nucleotide sequencing revealed a single open reading frame pos...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Harriott OT,Huber R,Stetter KO,Betts PW,Noll KM

    更新日期:1994-05-01 00:00:00

  • Purification and properties of phytate-specific phosphatase from Bacillus subtilis.

    abstract::An enzyme which liberates Pi from myo-inositol hexaphosphate (phytic acid) was shown to be present in culture filtrates of Bacillus subtilis. It was purified until it was homogeneous by ultracentrifugation, but it still showed two isozymes on polyacrylamide gel electrophoresis. The enzyme differed from other previousl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Powar VK,Jagannathan V

    更新日期:1982-09-01 00:00:00

  • Succinic Semialdehyde Promotes Prosurvival Capability of Agrobacterium tumefaciens.

    abstract:UNLABELLED:Succinic semialdehyde (SSA), an important metabolite of γ-aminobutyric acid (GABA), is a ligand of the repressor AttJ regulating the expression of the attJ-attKLM gene cluster in the plant pathogen Agrobacterium tumefaciens. While the response of A. tumefaciens to GABA and the function of attKLM have been ex...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wang C,Tang D,Gao YG,Zhang LH

    更新日期:2016-01-11 00:00:00

  • Envelope-associated folded chromosomes for Escherichia coli: variations under different physiological conditions.

    abstract::The folded chromosome of Escherichia coli has been investigated under various lysis and physiological conditions. A new gradient system was devised that allows excellent separation between unlysed cells and envelope-associated and envelope-free chromosomes. Isotope incorporation experiments showed that the fraction of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Korch C,Ovrebo S,Kleppe K

    更新日期:1976-08-01 00:00:00

  • Sequence and properties of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2.

    abstract::Pentaerythritol tetranitrate reductase, which reductively liberates nitrite from nitrate esters, is related to old yellow enzyme. Pentaerythritol tetranitrate reductase follows a ping-pong mechanism with competitive substrate inhibition by NADPH, is strongly inhibited by steroids, and is capable of reducing the unsatu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: French CE,Nicklin S,Bruce NC

    更新日期:1996-11-01 00:00:00

  • Lethality of the covalent linkage between mislocalized major outer membrane lipoprotein and the peptidoglycan of Escherichia coli.

    abstract::The major outer membrane lipoprotein (Lpp) of Escherichia coli possesses serine at position 2, which is thought to function as the outer membrane sorting signal, and lysine at the C terminus, through which Lpp covalently associates with peptidoglycan. Arginine (R) is present before the C-terminal lysine in the wild-ty...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yakushi T,Tajima T,Matsuyama S,Tokuda H

    更新日期:1997-05-01 00:00:00

  • Sorting out bacterial viability with optical tweezers.

    abstract::We have developed a method, using laser, optical tweezers and direct microscopic analysis of reproductive potential and membrane integrity, to assess single-cell viability in a stationary-phase Escherichia coli population. It is demonstrated here that a reduction in cell integrity, determined by using the fluorescent ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ericsson M,Hanstorp D,Hagberg P,Enger J,Nyström T

    更新日期:2000-10-01 00:00:00

  • Anaerobic regulation of the hydrogenase 1 (hya) operon of Escherichia coli.

    abstract::Using a transcriptional fusion to the lacZ gene, we have analyzed the anaerobic regulation of the hydrogenase 1 (hya) operon in response to different anaerobic growth conditions and to mutations in regulatory genes. We found that the transcription of the hya operon was induced when the growth condition was changed fro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Brøndsted L,Atlung T

    更新日期:1994-09-01 00:00:00


    abstract::Dienes, Louis (Massachusetts General Hospital, Boston, Mass.), Bojana M. Bandur, and Sarabelle Madoff. Development of L-type growth in Neisseria gonorrhoeae cultures. J. Bacteriol. 87:1471-1476. 1964.-Strains of Neisseria gonorrhoeae (22) were studied immediately after isolation from patients. After 24 to 48 hr of inc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1964-06-01 00:00:00

  • Characterization of the surface protein layers of the mosquito-pathogenic strains of Bacillus sphaericus.

    abstract::The protein surface layers on the cell walls of mosquito-pathogenic and nonpathogenic Bacillus sphaericus strains were studied by structural, biochemical, and serological methods. The surface structure of two representative insect-pathogenic strains had the form of a delicate linear array with a repeat interval of 5 n...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lewis LO,Yousten AA,Murray RG

    更新日期:1987-01-01 00:00:00

  • Subunits of succinyl-coenzyme A synthetase: coordination of production in Escherichia coli and discovery of a factor that precludes refolding.

    abstract::Succinyl-coenzyme A synthetase of Escherichia coli has an alpha 2 beta 2 subunit structure. By measuring reconstituted enzyme activity present after addition of purified alpha or beta subunits to cell extracts followed by refolding, we have shown that extracts contain no significant excess of either subunit species. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wolodko WT,Brownie ER,Bridger WA

    更新日期:1980-07-01 00:00:00

  • Escherichia coli fimbriae recognizing sialyl galactosides.

    abstract::Fimbriae recognizing sialyl galactosides (S fimbriae) were purified from an Escherichia coli strain. The S fimbriae were morphologically identical to type 1 and P fimbriae of E. coli and showed a hemagglutination that was abolished when erythrocytes were treated with neuraminidase. Hemagglutination by the purified fim...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Korhonen TK,Väisänen-Rhen V,Rhen M,Pere A,Parkkinen J,Finne J

    更新日期:1984-08-01 00:00:00

  • In vitro analysis of rates and spectra of mutations in a polymorphic region of the Rv0746 PE_PGRS gene of Mycobacterium tuberculosis.

    abstract::An assay modeled on a known polymorphism in the PE_PGRS9 gene of Mycobacterium tuberculosis was designed to assess the mutability of a sequence containing interspersed PGRS repeats. Application of the assay in Mycobacterium smegmatis revealed sequence plasticity: in addition to recapitulating the mutation on which it ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Machowski EE,Barichievy S,Springer B,Durbach SI,Mizrahi V

    更新日期:2007-03-01 00:00:00

  • Ultrastructural alterations associated with the growth of resistant Pseudomonas aeruginosa in the presence of benzalkonium chloride.

    abstract::Cells of Pseudomonas aeruginosa resistant to benzalkonium chloride (BC) underwent unique ultrastructural reorganizations when they were grown in the presence of 1 mg of BC/ml. The resistant cells usually contained a single, centrally positioned pseudovacuole. The pseudovacuole was surrounded by a diffuse substance tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hoffmann HP,Geftic SG,Gelzer J,Heymann H,Adair FW

    更新日期:1973-01-01 00:00:00

  • Isolation and characterization of mutants of colicin plasmids E1 and E2 after Mu bacteriophage infection.

    abstract::Cells colicinogenic for the colicin plasmids E1 or E2 (Col E1 and Col E2, respectively) were selected for a loss of colicin production after infection with bacteriophage Mu. Extrachromosomal deoxyribonucleic acid that was larger than the original colicin plasmids was found in such cells. A small insertion mutant in Co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Inselburg J

    更新日期:1974-08-01 00:00:00