Effect of growth medium on the relative polypeptide composition of cellular outer membrane and released outer membrane material in Escherichia coli.

abstract：:The occurrence of succinic dehydrogenase [succinic:(acceptor) oxidoreductase, EC 1.3.99.1] in membrane fractions of Micrococcus lysodeikticus was investigated. The enzyme could be purified 10-fold, by deoxycholate treatment. Butanol extraction of membranes yielded an active fraction, nonsedimentable at 130,000 x g for...

journal_title：Journal of bacteriology

authors： Pollock JJ,Linder R,Salton MR

更新日期：1971-07-01 00:00:00

abstract：:Nine natural isolates of Escherichia coli were examined, and the sequence of the entire 1,404 bases of the gnd gene (6-phosphogluconate dehydrogenase, EC 1.1.1.44) was determined. These isolates, along with E. coli K-12, constitute 10 strains for analysis. (The sequence of the E. coli K-12 gnd gene is known.) A total ...

journal_title：Journal of bacteriology

authors： Bisercić M,Feutrier JY,Reeves PR

更新日期：1991-06-01 00:00:00

abstract：:In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by co...

journal_title：Journal of bacteriology

authors： Wong PK,Popham D,Keener J,Kustu S

更新日期：1987-06-01 00:00:00

abstract：:The P(styA) promoter of Pseudomonas sp. strain Y2 controls expression of the styABCD genes, which are required for the conversion of styrene to phenylacetate, which is further catabolized by the products of two paa gene clusters. Two PaaX repressor proteins (PaaX1 and PaaX2) regulate transcription of the paa gene clus...

journal_title：Journal of bacteriology

authors： del Peso-Santos T,Bartolomé-Martín D,Fernández C,Alonso S,García JL,Díaz E,Shingler V,Perera J

更新日期：2006-07-01 00:00:00

abstract：:Mitospores or cysts of Allomyces macrogynus do not take up the glucose analogue 2-deoxyglucose. Uptake of 2-deoxyglucose by germlings begins at 25 min into germination, the start of the rhizoid stage, and increases in rate by approximately 50-fold until 100 min into germination. The rate remains constant from 100 to 2...

journal_title：Journal of bacteriology

authors： Burke DD

更新日期：1975-11-01 00:00:00

abstract：:The nucleotide messenger cyclic di-GMP (c-di-GMP) plays a central role in the regulation of motility, virulence, and biofilm formation in many pathogenic bacteria. EAL domain-containing phosphodiesterases are the major signaling proteins responsible for the degradation of c-di-GMP and maintenance of its cellular level...

journal_title：Journal of bacteriology

authors： Chen MW,Kotaka M,Vonrhein C,Bricogne G,Rao F,Chuah ML,Svergun D,Schneider G,Liang ZX,Lescar J

更新日期：2012-09-01 00:00:00

abstract：:The Escherichia coli htrD gene was originally isolated during a search for new genes required for growth at high temperature. Insertional inactivation of htrD leads to a pleiotropic phenotype characterized by temperature-sensitive growth in rich medium, H2O2 sensitivity, and sensitivity to cysteine. The htrD gene was ...

journal_title：Journal of bacteriology

authors： Delaney JM,Wall D,Georgopoulos C

更新日期：1993-01-01 00:00:00

abstract：:Studies on the active product of pepsin digestion of rabbit immunoglobulin G (IgG), F(ab')(2) produced from antiserum against Neurospora glutamate dehydrogenase, showed it to behave in the same way, in both antienzyme and precipitation experiments, as homologous IgG. It is proposed that the inhibition of glutamate deh...

journal_title：Journal of bacteriology

authors： Roberts DB

更新日期：1967-10-01 00:00:00

abstract：:A mutational change of the initiation codon to GUA was found to reduce, but not abolish, expression of the recJ gene of Escherichia coli. Specific mutations in translational initiation factor IF3 have been isolated as second-site suppressors of this GUA initiation codon mutation. One of these, infC135, with an arginin...

journal_title：Journal of bacteriology

authors： Haggerty TJ,Lovett ST

更新日期：1997-11-01 00:00:00

abstract：:The csgA gene produces an intercellular signal during fruiting body formation of the myxobacterium Myxococcus xanthus. Sporulating pseudorevertants were isolated to allow us to understand the mechanism by which CsgA is perceived by cells and used to regulate developmental gene expression. Two strains, LS559 and LS560,...

journal_title：Journal of bacteriology

authors： Lee K,Shimkets LJ

更新日期：1994-04-01 00:00:00

abstract：:Some Escherichia coli strains with impaired cell division form branched cells at high frequencies during certain growth conditions. Here, we show that neither FtsI nor FtsZ activity is required for the development of branches. Buds did not form at specific positions along the cell surface during high-branching conditi...

journal_title：Journal of bacteriology

authors： Gullbrand B,Akerlund T,Nordström K

更新日期：1999-11-01 00:00:00

abstract：:DNA polymerase activities in fractionated cell extract of Aeropyrum pernix, a hyperthermophilic crenarchaeote, were investigated. Aphidicolin-sensitive (fraction I) and aphidicolin-resistant (fraction II) activities were detected. The activity in fraction I was more heat stable than that in fraction II. Two different ...

journal_title：Journal of bacteriology

authors： Cann IK,Ishino S,Nomura N,Sako Y,Ishino Y

更新日期：1999-10-01 00:00:00

abstract：:MntH, a bacterial homolog of mammalian natural resistance associated macrophage protein 1 (Nramp1), is a primary transporter for Mn(2+) influx in Salmonella enterica serovar Typhimurium and Escherichia coli. S. enterica serovar Typhimurium MntH contributes to H(2)O(2) resistance and is important for full virulence. Co...

journal_title：Journal of bacteriology

authors： Kehres DG,Janakiraman A,Slauch JM,Maguire ME

更新日期：2002-06-01 00:00:00

abstract：:A segment of early RNA from Bacillus subtilis bacteriophage SP82 was shown to function as a 5' stabilizer in B. subtilis. Several heterologous RNA sequences were stabilized by the presence of the SP82 sequence at the 5' end, and expression of downstream coding sequences was increased severalfold. The SP82 RNA segment ...

journal_title：Journal of bacteriology

authors： Hue KK,Cohen SD,Bechhofer DH

更新日期：1995-06-01 00:00:00

abstract：:Seligman, Stephen J. (University of California, Los Angeles) and William L. Hewitt. Kinetics of the action of ampicillin on Escherichia coli. J. Bacteriol. 85:1160-1164. 1963.-The curve of the number of viable Escherichia coli after exposure to ampicillin can be divided into three phases: a lag phase, a rapid bacteric...

journal_title：Journal of bacteriology

authors： SELIGMAN SJ,HEWITT WL

更新日期：1963-05-01 00:00:00

abstract：:Synthetic analogs of enterochelin (enterobactin) were tested for their ability to support the growth of Escherichia coli K-12 under iron-limiting conditions. The cyclic compound MECAM [1,3,5-N.N'; N"-tris-(2,3-dihydroxybenzoyl)-triamino-methylbenzene] and its N-methyl derivative Me3MECAM promoted growth, whereas the 2...

journal_title：Journal of bacteriology

authors： Heidinger S,Braun V,Pecoraro VL,Raymond KN

更新日期：1983-01-01 00:00:00

abstract：:5-Azacytidine was found to be bactericidal to Escherichia coli carrying plasmids specifying EcoRII restriction-modification systems, but not to the same strains lacking these plasmids. Of other base analogs tested, only 5(beta-D-ribofuranosyl)isocytidine had similar, although weaker, effects. Plasmids that had lost th...

journal_title：Journal of bacteriology

authors： Friedman S

更新日期：1982-07-01 00:00:00

abstract：:Bacillus subtilis cwlD and dacB mutants produce spore peptidoglycan (PG) with increased cross-linking but with little change in spore core hydration compared to the wild type. A cwlD dacB double mutant produced spores with a two- to fourfold greater increase in PG cross-linking and novel muropeptides containing glycin...

journal_title：Journal of bacteriology

authors： Popham DL,Meador-Parton J,Costello CE,Setlow P

更新日期：1999-10-01 00:00:00

abstract：:The first generalized transducing bacteriophage reported for Bacillus megaterium has been characterized. Optimum conditions for lysate production and transduction procedures were established so that transducing frequencies of 8 x 10(-6) and higher are now possible. The phage, MP13, has a head diameter of 97 nm and a c...

journal_title：Journal of bacteriology

authors： Vary PS,Garbe JC,Franzen M,Frampton EW

更新日期：1982-03-01 00:00:00

abstract：:Rickettsia typhi, the causative agent of murine typhus, is an obligate intracellular bacterium with a life cycle involving both vertebrate and invertebrate hosts. Here we present the complete genome sequence of R. typhi (1,111,496 bp) and compare it to the two published rickettsial genome sequences: R. prowazekii and ...

journal_title：Journal of bacteriology

authors： McLeod MP,Qin X,Karpathy SE,Gioia J,Highlander SK,Fox GE,McNeill TZ,Jiang H,Muzny D,Jacob LS,Hawes AC,Sodergren E,Gill R,Hume J,Morgan M,Fan G,Amin AG,Gibbs RA,Hong C,Yu XJ,Walker DH,Weinstock GM

更新日期：2004-09-01 00:00:00

abstract：:Expression of the 146-kilodalton (kDa) extracellular glucoamylase by the budding yeast Schwanniomyces castellii is induced by maltose and starch. By use of antiglucoamylase antisera, we found that this expression was regulated at the level of the mRNA, taking place within 30 min after exposure of yeast cells to the re...

journal_title：Journal of bacteriology

authors： Dowhanick TM,Russell I,Scherer SW,Stewart GG,Seligy VL

更新日期：1990-05-01 00:00:00

abstract：:Gaspar, Andrew J. (Fort Detrick, Frederick, Md.), Hugh B. Tresselt, and Martha K. Ward. New solid medium for enhanced growth of Pasteurella tularensis. J. Bacteriol. 82:564-569. 1961.-The purpose of this work was to develop a solid medium for more rapid growth of Pasteurella tularensis, especially from small inocula c...

journal_title：Journal of bacteriology

authors： GASPAR AJ,TRESSELT HB,WARD MK

更新日期：1961-10-01 00:00:00

abstract：:Lipopolysaccharide (LPS) was isolated from the unicellular blue-green bacterium Agmenellum quadruplicatum using the procedure of Westphal and Jann (1965). It was composed of a lipid A and polysaccharide region suggesting a similarity to other gram-negative LPSs. Chemical analyses demonstrated the presence of glucose, ...

journal_title：Journal of bacteriology

authors： Buttke TM,Ingram LO

更新日期：1975-12-01 00:00:00

abstract：:The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is a multifunctional autotransporter protein that mediates bacterial aggregation and biofilm formation, as well as adhesion and invasion of cultured epithelial cells. To elucidate the structure-function relationships of AIDA-I, we performed transposon...

journal_title：Journal of bacteriology

authors： Charbonneau ME,Mourez M

更新日期：2007-12-01 00:00:00

abstract：:Burkholderia cenocepacia strain K56-2, a representative of the Burkholderia cepacia complex, is part of the epidemic and clinically problematic ET12 lineage. The strain produced plant tissue watersoaking (ptw) on onion tissue, which is a plant disease-associated trait. Using plasposon mutagenesis, mutants in the ptw p...

journal_title：Journal of bacteriology

authors： Engledow AS,Medrano EG,Mahenthiralingam E,LiPuma JJ,Gonzalez CF

更新日期：2004-09-01 00:00:00

abstract：:Unlike mutations to streptomycin resistance, which restrict the function of an SU II amber suppressor, mutations to spectinomycin resistance have no restrictive effect. Furthermore, mutations to spectinomycin resistance counteract the restrictive action of streptomycin-resistant mutations when combined in a single str...

journal_title：Journal of bacteriology

authors： Kuwano M,Endo H,Ohnishi Y

更新日期：1969-02-01 00:00:00

abstract：:Acidified phenol extracts prepared from competent cultures of a group H Streptococcus strain Wicky made competent with competence factor derived from cultures of another group H Streptococcus, strain Challis, showed a difference in polyacrylamide-gel protein patterns when compared to extracts prepared from noncompeten...

journal_title：Journal of bacteriology

authors： Ranhand JM,Theodore TS,Cole RM

更新日期：1970-10-01 00:00:00

abstract：:Haemophilus influenzae is a common gram-negative pathogen that initiates infection by colonizing the upper respiratory tract epithelium. In previous work, we reported the isolation of a locus involved in expression of short, thin surface fibrils by H. influenzae type b and presented evidence that surface fibrils promo...

journal_title：Journal of bacteriology

authors： St Geme JW 3rd,Cutter D,Barenkamp SJ

更新日期：1996-11-01 00:00:00

abstract：:The activation of the Pm promoter of the meta operon of the TOL plasmid of Pseudomonas putida by its cognate XylS activator protein in the presence and absence of benzoate inducers has been examined in specialized Escherichia coli strains carrying Pm-lacZ fusions and the xylS gene in different configurations in which ...

journal_title：Journal of bacteriology

authors： Kessler B,Herrero M,Timmis KN,de Lorenzo V

更新日期：1994-06-01 00:00:00

abstract：:Unique lipids found in Azotobacter vinelandii cysts are derived primarily from beta-hydroxybutyrate used to induce encystment. Tracer studies with beta-[14C]hydroxybutyrate showed that the biosynthesis of these compounds during encystment began at 8 to 12 h after induction and reached maximal levels after 2 days, Seve...

journal_title：Journal of bacteriology

authors： Su CJ,Sadoff HL

更新日期：1981-07-01 00:00:00