On the origin of branches in Escherichia coli.

Abstract:

:Some Escherichia coli strains with impaired cell division form branched cells at high frequencies during certain growth conditions. Here, we show that neither FtsI nor FtsZ activity is required for the development of branches. Buds did not form at specific positions along the cell surface during high-branching conditions. Antibiotics affecting cell wall synthesis had a positive effect on branch formation in the case of ampicillin, cephalexin, and penicillin G, whereas mecillinam and D-cycloserine had no substantial effect. Altering the cell morphology by nutritional shifts showed that changes in morphology preceded branching, indicating that the cell's physiological state rather than specific medium components induced branching. Finally, there was no increased probability for bud formation in the daughters of a cell with a bud or branch, showing that bud formation is a random event. We suggest that branch formation is caused by abnormalities in cell wall elongation rather than by aberrant cell division events.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Gullbrand B,Akerlund T,Nordström K

doi

10.1128/JB.181.21.6607-6614.1999

keywords:

subject

Has Abstract

pub_date

1999-11-01 00:00:00

pages

6607-14

issue

21

eissn

0021-9193

issn

1098-5530

journal_volume

181

pub_type

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