Abstract:
:Lipopolysaccharide (LPS) was isolated from the unicellular blue-green bacterium Agmenellum quadruplicatum using the procedure of Westphal and Jann (1965). It was composed of a lipid A and polysaccharide region suggesting a similarity to other gram-negative LPSs. Chemical analyses demonstrated the presence of glucose, rhamnose, mannose, and xylose in the polysaccharide region, as well as 2-keto-3-deoxyoctonate, glucosamine, and phosphorous in the lipid A. Studies on the lipid composition revealed the presence of palmitic, behenic, and three beta-hydroxy fatty acids. A new procedure for thin-layer chromatography of bacterial LPSs was used to compare LPS from A. quadruplicatum to other gram-negative organisms. The method is capable of distinguishing between LPSs of different bacteria as well as between the wild-type organism and mutated forms unable to synthesize complete LPS. A comparison of LPS from A. quadruplicatum to Escherichia coli and Salmonella typhimurium demonstrated that, although the blue-green LPS was rather similar to that of the Enterobacteriaceae, distinct differences also existed. However, when several cell division mutants of A. quadruplicatum were compared chromatographically to the parent strain BG-1, no differences were observed. This suggests that cell division mutations in A. quadruplicatum are not associated with changes in the LPS.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Buttke TM,Ingram LOdoi
10.1128/JB.124.3.1566-1573.1975subject
Has Abstractpub_date
1975-12-01 00:00:00pages
1566-73issue
3eissn
0021-9193issn
1098-5530journal_volume
124pub_type
杂志文章abstract::Ralston, D. J. (University of California, Berkeley) and M. D. Perry. Inhibitory action of phage K on staphylococcal dehydrogenases. II. Its possible relationship to sensitization and cell lysis. J. Bacteriol. 86:740-748. 1963.-By measuring the reduction of the dye triphenyl tetrazolium chloride to the insoluble red fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.4.740-748.1963
更新日期:1963-10-01 00:00:00
abstract:UNLABELLED:The σE envelope stress response is an essential signal transduction pathway which detects and removes mistargeted outer membrane (OM) β-barrel proteins (OMPs) in the periplasm of Escherichia coli It relies on σE, an alternative sigma factor encoded by the rpoE gene. Here we report a novel mutation, a nucleot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00340-16
更新日期:2016-08-11 00:00:00
abstract::Frampton, E. W. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston), and B. R. Brinkley. Evidence of lysogeny in derivatives of Escherichia coli. J. Bacteriol. 90:446-452. 1965.-Exposure of cultures of several thymine-requiring derivatives of Escherichia coli strain 15 to radiation [X rays o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.2.446-452.1965
更新日期:1965-08-01 00:00:00
abstract::Klite, P. D. (Middle America Research Unit, Balboa Heights, Canal Zone). Intestinal bacterial flora and transit time of three neotropical bat species. J. Bacteriol. 90:375-379. 1965.-Quantitative studies on the intestinal bacterial flora of three neotropical bat species revealed the following average bacterial populat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.2.375-379.1965
更新日期:1965-08-01 00:00:00
abstract::Several Pseudomonas solanacearum strains which produced no detectable extracellular polysaccharide (EPS) in planta had been reported to remain highly virulent when tested at high inoculum concentrations (P. Xu, M. Iwata, S. Leong, and L. Sequeira, J. Bacteriol. 172:3946-3951, 1990; P. Xu, S. Leong, and L. Sequeira, J....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.3.1068-1071.1992
更新日期:1992-02-01 00:00:00
abstract::A Bordetella bronchiseptica iron transport mutant was isolated following an enrichment procedure based on streptonigrin resistance. The mutant displayed a growth defect on iron-restricted medium containing ferric alcaligin as the sole iron source. In addition to the apparent inability to acquire iron from the sideroph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.4.862-870.1998
更新日期:1998-02-01 00:00:00
abstract::In vitro-packaged cosmid libraries of DNA from the bacterium Xanthomonas campestris pv. malvacearum were restricted 200- to 1,000-fold when introduced into Mcr+ strains of Escherichia coli compared with restriction in the Mcr- strain HB101. Restriction was predominantly associated with the mcrBC+ gene in E. coli. A pl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.20.6421-6427.1991
更新日期:1991-10-01 00:00:00
abstract::Staphylococcus aureus responds to changing extracellular environments in part by adjusting its proteome through alterations of transcriptional priorities and selective degradation of the preexisting pool of proteins. In Bacillus subtilis, the proteolytic adaptor protein MecA has been shown to play a role in assisting ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02222-14
更新日期:2014-12-01 00:00:00
abstract::A conditionally germination-defective mutant of Neurospora crassa has been found to be partially curable by ferricrocin and other siderophores. The mutant conidia rapidly lose their membrane-bound siderophores when suspended in buffer or growth media. Germination is consequently delayed unless large numbers of conidia...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.3.1042-1044.1977
更新日期:1977-12-01 00:00:00
abstract::Quorum sensing is involved in the regulation of multicellular behavior through communication via small molecules. Given the high number and diversity of the gastrointestinal microbiota, it is postulated that members of this community communicate to coordinate a variety of adaptive processes. AI-2 is suggested to be a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01394-06
更新日期:2007-02-01 00:00:00
abstract::The rates of synthesis of Aerobacter aerogenes nucleic acids were estimated during incubation of the bacteria in a Mg(++)-free medium. Deoxyribonucleic acid (DNA) synthesized during Mg(++) starvation, or in the preceding exponential growth, remained acid-precipitable for 2.5 hr before breaking down to acid-soluble pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.345-356.1967
更新日期:1967-01-01 00:00:00
abstract::Mycobacterium tuberculosis can persist for many years within host lung tissue without causing clinical disease. Little is known about the state in which the bacilli survive, although it is frequently referred to as dormancy. Some evidence suggests that cells survive in nutrient-deprived stationary phase. Therefore, we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.1.270-283.1999
更新日期:1999-01-01 00:00:00
abstract::Somerson, Norman L. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), and M. K. Cook. Suppression of Rous sarcoma virus growth in tissue cultures by Mycoplasma orale. J. Bacteriol. 90:534-540. 1965.-An agent which produced cell destruction in human diploid and chick-embryo fibroblasts was isolate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.2.534-540.1965
更新日期:1965-08-01 00:00:00
abstract::The endophytic diazotroph Gluconacetobacter diazotrophicus secretes a constitutively expressed levansucrase (LsdA, EC 2.4.1.10) to utilize plant sucrose. LsdA, unlike other extracellular levansucrases from gram-negative bacteria, is transported to the periplasm by a signal-peptide-dependent pathway. We identified an u...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.15.5031-5039.2004
更新日期:2004-08-01 00:00:00
abstract::CsgA mutants of Myxococcus xanthus appear to be defective in producing an extracellular molecule essential for the developmental behaviors of this bacterium. The csgA gene encodes a 17.7-kilodalton polypeptide whose function and cellular location were investigated with immunological probes. Large quantities of the Csg...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.5299-5306.1990
更新日期:1990-09-01 00:00:00
abstract::Biofilm formation in Staphylococcus epidermidis is dependent upon the ica operon-encoded polysaccharide intercellular adhesin, which is subject to phase-variable and environmental regulation. The icaR gene, located adjacent to the ica operon, appears to be a member of the tetR family of transcriptional regulators. In ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.16.4400-4408.2002
更新日期:2002-08-01 00:00:00
abstract::Streptococcus mutans transports glucose via the phosphoenolpyruvate (PEP)-dependent sugar phosphotransferase system (PTS). Earlier studies indicated that an alternate glucose transport system functions in this organism under conditions of high growth rates, low pH, or excess glucose. To identify this system, S. mutans...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.9.2251-2258.1995
更新日期:1995-05-01 00:00:00
abstract::Lactococcus lactis subsp. lactis ML3 contains high pools of proline or betaine when grown under conditions of high osmotic strength. These pools are created by specific transport systems. A high-affinity uptake system for glycine betaine (betaine) with a Km of 1.5 microM is expressed constitutively. The activity of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.17.5438-5444.1993
更新日期:1993-09-01 00:00:00
abstract::The yeast two-hybrid assay revealed that Bacillus subtilis DnaD, a possible component of the primosome and required for replication initiation, interacted with DnaA and DnaD itself. The mutant DnaD23 was incapable of interacting with DnaA but retained interaction with the wild-type DnaD. These results suggest that int...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.6.2148-2150.2001
更新日期:2001-03-01 00:00:00
abstract::Carere, A., and Isabella Spada-Sermonti (Istituto Superiore di Sanità, Rome, Italy). Nutritional mutations and transduction by ultraviolet-inactivated phage in Staphylococcus aureus. J. Bacteriol. 88:226-232. 1964.-By use of a semisynthetic medium, various nutritional mutants of Staphylococcus aureus 80 were isolated....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.1.226-232.1964
更新日期:1964-07-01 00:00:00
abstract::In a search for genes that produce hypersensitivity to cadmium salts in Escherichia coli, random transposon mutagenesis with TnphoA was used. One of the mutant strains obtained was sensitive to Cd2+ and Zn2+. Sequence analysis showed that the TnphoA insertion was located in the dsbA gene coding for a periplasmic prote...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.8.2769-2771.1997
更新日期:1997-04-01 00:00:00
abstract::Das, S. K. (University of Calcutta, Calcutta, India) and G. C. Chatterjee. Pyrithiamine adaptation of Staphylococcus aureus. I. Adaptation and carbohydrate utilization. J. Bacteriol. 83:1251-1259. 1962.-Staphylococcus aureus has been adapted to pyrithiamine, a thiamine analogue; as a result of this adaptation, the col...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.6.1251-1259.1962
更新日期:1962-06-01 00:00:00
abstract::Escherichia coli has been used as a platform host for studying the production of free fatty acids (FFA) and other energy-dense compounds useful in biofuel applications. Most of the FFA produced by E. coli are found extracellularly. This finding suggests that a mechanism for transport across the cell envelope exists, y...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01477-12
更新日期:2013-01-01 00:00:00
abstract::Mutants that require exogenous D-glucosamine for growth were isolated from Saccharomyces cerevisiae X2180-1A after ethyl methane sulfonate mutagenesis. Class A auxotrophs fail to grow on yeast extract-peptone-dextrose and minimal media, whereas class B auxotrophs grow on minimal medium and readily revert to grow on ye...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1545-1557.1975
更新日期:1975-12-01 00:00:00
abstract::Mycoplasma iowae is associated mainly with reduced hatchability in turkeys and is well known for the unusual ability of phenotypic variation in the Mycoplasma surface components as well as a relative resistance to heat, bile salts, and many antimicrobials. A subset of unique genes and a gene cluster responsible for th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06297-11
更新日期:2012-01-01 00:00:00
abstract::Corynebacterium diphtheriae, a Gram-positive, aerobic bacterium, is the causative agent of diphtheria and cutaneous infections. While mechanisms required for heme iron acquisition are well known in C. diphtheriae, systems involved in the acquisition of other metals such as zinc and manganese remain poorly characterize...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00051-18
更新日期:2018-04-24 00:00:00
abstract::When 100 group A streptococcus isolates were screened, drs, a variant of sic, was identified in emm12 and emm55 isolates. Molecular characterization showed that the drs gene sequence is highly conserved, unlike the sic gene sequence. However, the variation in gene size observed was due to the presence of extra interna...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01619-06
更新日期:2007-04-01 00:00:00
abstract::Strains of Bacillus thuringiensis such as B. thuringiensis subsp. israelensis (ONR-60A) and B. thuringiensis subsp. morrisoni (PG-14) pathogenic for mosquito larvae produce a complex parasporal body consisting of several protein endotoxins synthesized during sporulation that form an aggregate of crystalline inclusions...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06095-11
更新日期:2012-03-01 00:00:00
abstract::Anaerobic microbial toluene catabolism is initiated by addition of fumarate to the methyl group of toluene, yielding (R)-benzylsuccinate as first intermediate, which is further metabolized via beta-oxidation to benzoyl-coenzyme A (CoA) and succinyl-CoA. A specific succinyl-CoA:(R)-benzylsuccinate CoA-transferase activ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.14.4288-4295.2001
更新日期:2001-07-01 00:00:00
abstract::A globomycin-resistant mutant of Escherichia coli was found to produce a precursor of the major outer membrane lipoprotein (prolipoprotein), in which the glycine residue at position 14 within the signal peptide was replaced by an aspartic acid residue. The same mutation has been reported by Lin et al. (Proc. Natl. Aca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.1.407-411.1983
更新日期:1983-07-01 00:00:00