Use of cloned DNA methylase genes to increase the frequency of transfer of foreign genes into Xanthomonas campestris pv. malvacearum.


:In vitro-packaged cosmid libraries of DNA from the bacterium Xanthomonas campestris pv. malvacearum were restricted 200- to 1,000-fold when introduced into Mcr+ strains of Escherichia coli compared with restriction in the Mcr- strain HB101. Restriction was predominantly associated with the mcrBC+ gene in E. coli. A plasmid (pUFR052) encoding the XmaI and XmaIII DNA methylases was isolated from an X. campestris pv. malvacearum library by a screening procedure utilizing Mcr+ and Mcr- E. coli strains. Transfer of plasmids from E. coli strains to X. campestris pv. malvacearum by conjugation was enhanced by up to five orders of magnitude when the donor cells contained pUFR052 as well as the plasmid to be transferred. Subcloning of pUFR052 revealed that at least two regions of the plasmid were required for full modification activity. Use of such modifier plasmids is a simple, novel method that may allow the efficient introduction of genes into any organism in which restriction systems provide a potent barrier to such gene transfer.


J Bacteriol


Journal of bacteriology


De Feyter R,Gabriel DW





Has Abstract


1991-10-01 00:00:00












  • Neurospora mutant exhibiting hyperproduction of amylase and invertase.

    abstract::A mutant strain of Neurospora crassa has been isolated which is derepressed for amylase and beta-fructofuranosidase (invertase). Large amounts of the two enzymes were secreted into the culture medium upon depletion of exogenous carbon source. The resulting increases of the two extracellular enzymes were prevented by a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gratzner H,Sheehan DN

    更新日期:1969-02-01 00:00:00

  • Consequences of glycerol deprivation on the synthesis of membrane components in a glycerol auxotroph of Staphylococcus aureus.

    abstract::In a glycerol auxotroph of Staphylococcus aureus, the deprivation of glycerol affected the formation of certain membrane components. (i) There was synthesis of fatty acids at the predeprivation rate even though the fatty acids synthesized accumulated as free fatty acids rather than as esterified fatty acids; (ii) ther...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ray PH,Lillich TT,White DC

    更新日期:1972-10-01 00:00:00

  • IS1634, a novel insertion element creating long, variable-length direct repeats which is specific for Mycoplasma mycoides subsp. mycoides small-colony type.

    abstract::A new insertion sequence, IS1634, has been identified in Mycoplasma mycoides subsp. mycoides small-colony type (SC). IS1634 shows structural and functional similarities to IS1549 of Mycobacterium smegmatis and with it seems to form a new class or family of insertion sequences. IS1634 has a size of 1,872 bp, including ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vilei EM,Nicolet J,Frey J

    更新日期:1999-02-01 00:00:00

  • High-frequency variation in Mycoplasma pulmonis colony size.

    abstract::Heterogeneity in colony size of the murine pathogen Mycoplasma pulmonis was examined. Subcloning experiments showed that colony size variation resulted from high-frequency genetic changes. About 3% of the colonies from any given subclone were variants, with as much as a fourfold change in colony diameter. When the var...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Dybvig K,Simecka JW,Watson HL,Cassell GH

    更新日期:1989-09-01 00:00:00

  • mgpS, a complex regulatory locus involved in the transcriptional control of the puc and puf operons in Rhodobacter sphaeroides 2.4.1.

    abstract::A new method has been developed in order to select mutants showing decreased puc operon transcription in Rhodobacter sphaeroides 2.4.1. A transcriptional fusion of a promoterless fragment derived from the sacB gene, encoding the levansucrase from Bacillus subtilis, to the upstream regulatory region of the puc operon h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sabaty M,Kaplan S

    更新日期:1996-01-01 00:00:00

  • The final step of peptidoglycan subunit assembly in Escherichia coli occurs in the cytoplasm.

    abstract::The murG gene of Escherichia coli encodes the N-acetylglucosaminyltransferase responsible for the final step in the formation of the lipid-linked disaccharide-pentapeptide subunit of peptidoglycan. Using trypsin to probe maxicell spheroplasts, we show that this enzyme is peripherally associated with the inner face of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bupp K,van Heijenoort J

    更新日期:1993-03-01 00:00:00

  • Acetohydroxy acid synthase I is required for isoleucine and valine biosynthesis by Salmonella typhimurium LT2 during growth on acetate or long-chain fatty acids.

    abstract::Salmonella typhimurium LT2 normally expresses two acetohydroxy acid synthases (AHAS I and AHAS II). The function of AHAS I in this organism was unclear, since AHAS I-deficient (ilvBN) mutants of LT2 grew well on glucose or succinate minimal media, whereas AHAS II-deficient (ilvGM) mutants requried isoleucine for norma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Dailey FE,Cronan JE Jr,Maloy SR

    更新日期:1987-02-01 00:00:00

  • Identification and structure of the nasR gene encoding a nitrate- and nitrite-responsive positive regulator of nasFEDCBA (nitrate assimilation) operon expression in Klebsiella pneumoniae M5al.

    abstract::Klebsiella pneumoniae can use nitrate and nitrite as sole nitrogen sources through the nitrate assimilatory pathway. The structural genes for assimilatory nitrate and nitrite reductases together with genes necessary for nitrate transport form an operon, nasFEDCBA. Expression of the nasF operon is regulated both by gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Goldman BS,Lin JT,Stewart V

    更新日期:1994-08-01 00:00:00

  • Subapical wall synthesis and wall thickening induced by cycloheximide in hyphae of Aspergillus nidulans.

    abstract::Hyphae of Aspergillus nidulans continued to synthesize all the major polysaccharide components of the cell wall when cycloheximide was added to cultures. In the presence of cycloheximide, hyphae did not elongate, but electron microscopy showed that the walls became thicker around the cell. The conclusion that cyclohex...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sternlicht E,Katz D,Rosenberger RF

    更新日期:1973-05-01 00:00:00

  • Polyphosphate and orthophosphate content of Nitrosomonas europaea as a function of growth.

    abstract::After inoculation of a stationary-phase culture of Nitrosomonas europaea into fresh growth solution, the cell-associated orthophosphate increased rapidly to 800 mumoles/g (wet weight), whereas the acid-insoluble long-chain polyphosphate content decreased rapidly to 22 mumoles/g. As growth proceeded, the orthophosphate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Terry KR,Hooper AB

    更新日期:1970-07-01 00:00:00

  • Transcriptional Landscape and Regulatory Roles of Small Noncoding RNAs in the Oxidative Stress Response of the Haloarchaeon Haloferax volcanii.

    abstract::Haloarchaea in their natural environment are exposed to hypersalinity, intense solar radiation, and desiccation, all of which generate high levels of oxidative stress. Previous work has shown that haloarchaea are an order of magnitude more resistant to oxidative stress than most mesophilic organisms. Despite this resi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gelsinger DR,DiRuggiero J

    更新日期:2018-04-09 00:00:00

  • Regulation of Salmonella enterica serovar Typhimurium mntH transcription by H(2)O(2), Fe(2+), and Mn(2+).

    abstract::MntH, a bacterial homolog of mammalian natural resistance associated macrophage protein 1 (Nramp1), is a primary transporter for Mn(2+) influx in Salmonella enterica serovar Typhimurium and Escherichia coli. S. enterica serovar Typhimurium MntH contributes to H(2)O(2) resistance and is important for full virulence. Co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kehres DG,Janakiraman A,Slauch JM,Maguire ME

    更新日期:2002-06-01 00:00:00

  • Autoregulation of Escherichia coli purR requires two control sites downstream of the promoter.

    abstract::The expression of Escherichia coli purR, which encodes the pur regulon repressor protein, is autoregulated. Autoregulation at the level of transcription requires two operator sites, designated purRo1 and purRo2 (O1 and O2). Operator O1 is in the region of DNA between the transcription start site and the site for trans...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rolfes RJ,Zalkin H

    更新日期:1990-10-01 00:00:00

  • Transcription of two sigma 70 homologue genes, sigA and sigB, in stationary-phase Mycobacterium tuberculosis.

    abstract::The sigA and sigB genes of Mycobacterium tuberculosis encode two sigma 70-like sigma factors of RNA polymerase. While transcription of the sigA gene is growth rate independent, sigB transcription is increased during entry into stationary phase. The sigA gene transcription is unresponsive to environmental stress but th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hu Y,Coates AR

    更新日期:1999-01-01 00:00:00

  • Effects of high incubation temperature upon the cell wall of Escherichia coli.

    abstract::Hoffman, Heiner (New York University, New York, N.Y.), Jon Valdina, and Michael E. Frank. Effects of high incubation temperature upon the cell wall of Escherichia coli. J. Bacteriol. 91:1635-1637. 1966.-Escherichia coli cells grown at 45 C were found to swell when suspended in distilled water, and were broken down whe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hoffman H,Valdina J,Frank ME

    更新日期:1966-04-01 00:00:00

  • A survey of the Mycoplasma genitalium genome by using random sequencing.

    abstract::A total of 508 random clones from five Mycoplasma genitalium genomic libraries were partially sequenced and analyzed. This resulted in the identification of 291 unique contigs. Sequence information from these clones (100,993 nucleotides), representing approximately 17% of this pathogen's genome, was analyzed by compar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Peterson SN,Hu PC,Bott KF,Hutchison CA 3rd

    更新日期:1993-12-01 00:00:00

  • Methionine sulfoxide reductase from the hyperthermophilic archaeon Thermococcus kodakaraensis, an enzyme designed to function at suboptimal growth temperatures.

    abstract::Methionine sulfoxide reductase (Msr) catalyzes the thioredoxin-dependent reduction and repair of methionine sulfoxide (MetO). Although Msr genes are not present in most hyperthermophile genomes, an Msr homolog encoding an MsrA-MsrB fusion protein (MsrAB(Tk)) was present on the genome of the hyperthermophilic archaeon ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Fukushima E,Shinka Y,Fukui T,Atomi H,Imanaka T

    更新日期:2007-10-01 00:00:00

  • Identification of the minimum regulatory region of a Myxococcus xanthus A-signal-dependent developmental gene.

    abstract::Developmental expression of the Myxococcus xanthus gene 4521 requires extracellular A-signal. This signal is generated in response to nutrient limitation and functions in cell density sensing. To identify the upstream limit of the minimum region required in vivo for A-signal-dependent 4521 expression, a 5' deletion an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gulati P,Xu D,Kaplan HB

    更新日期:1995-08-01 00:00:00

  • Characterization of the functional sites in the oriT region involved in DNA transfer promoted by sex factor plasmid R100.

    abstract::We have previously identified three sites, named sbi, ihfA, and sbyA, specifically recognized or bound by the TraI, IHF, and TraY proteins, respectively; these sites are involved in nicking at the origin of transfer, oriT, of plasmid R100. In the region next to these sites, there exists the sbm region, which consists ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Abo T,Ohtsubo E

    更新日期:1995-08-01 00:00:00

  • Cation-activated nucleotidase in cell envelopes of a marine bacterium.

    abstract::Isolated cell envelopes of a marine bacterium, M.B.3, have been prepared which possess a nonspecific, cation-activated nucleotidase. The cell envelope comprises approximately 35% (dry weight) of the whole cell and contains protein, 60.2%; lipids, 20.7%; hexose, 3.4%; and ribonucleic acid, 4.6%. No deoxyribonucleic aci...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Thompson J,Green ML,Happold FC

    更新日期:1969-09-01 00:00:00

  • Interaction of the exr and lon genes in Escherichia coli.

    abstract::Strains of Escherichia coli carrying the gene lon typically produced excess capsular polysaccharide, and were sensitive to ultraviolet light (UV) irradiation, thymine starvation, and nalidixic acid, forming long filaments after these treatments. Sensitivity was reduced by a number of posttreatments. In the presence of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Donch J,Green MH,Greenberg J

    更新日期:1968-11-01 00:00:00

  • Escherichia coli mutants with an altered sensitivity to cecropin D.

    abstract::Cecropins are a family of small, basic antibacterial polypeptides which can be isolated from pupae of immunized Lepidoptera. They are active against both gram-negative and gram-positive bacteria. We studied a mutant of Escherichia coli, strain SB1004, which is more sensitive to cecropin D than is the parental strain. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sidén I,Boman HG

    更新日期:1983-04-01 00:00:00

  • Three new regulators of swarming in Vibrio parahaemolyticus.

    abstract::Movement on surfaces, or swarming motility, is effectively mediated by the lateral flagellar (laf) system in Vibrio parahaemolyticus. Expression of laf is induced by conditions inhibiting rotation of the polar flagellum, which is used for swimming in liquid. However, not all V. parahaemolyticus isolates swarm proficie...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jaques S,McCarter LL

    更新日期:2006-04-01 00:00:00

  • Quorum sensing in Vibrio fischeri: evidence that S-adenosylmethionine is the amino acid substrate for autoinducer synthesis.

    abstract::Synthesis of the autoinducer signal involved in the cell density-dependent activation of Vibrio fischeri luminescence is directed by luxI. The autoinducer is N-(3-oxohexanoyl)homoserine lactone, and little is known about its synthesis. We have measured autoinducer synthesis by amino acid auxotrophs of Escherichia coli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hanzelka BL,Greenberg EP

    更新日期:1996-09-01 00:00:00

  • Identification and characterization of a new lipoprotein, NlpI, in Escherichia coli K-12.

    abstract::We report here the identification of a new lipoprotein, NlpI, in Escherichia coli K-12. The NlpI structural gene (nlpI) is located between the genes pnp (polynucleotide phosphorylase) and deaD (RNA helicase) at 71 min on the E. coli chromosome. The nlpI gene encodes a putative polypeptide of approximately 34 kDa, and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ohara M,Wu HC,Sankaran K,Rick PD

    更新日期:1999-07-01 00:00:00


    abstract::Treick, R. W. (Indiana University, Bloomington), and W. A. Konetzka. Physiological state of Escherichia coli and the inhibition of deoxyribonucleic acid synthesis by phenethyl alcohol. J. Bacteriol. 88:1580-1584. 1964.-The effects of concentration of phenethyl alcohol (PEA) and the physiological state of the cells on ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1964-12-01 00:00:00

  • Genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5T.

    abstract::Oligotropha carboxidovorans OM5(T) (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium with the capability to utilize carbon monoxide, carbon dioxide, and hydrogen. It is also capable of heterotrophic growth under appropriate environmental conditions. Here we report the annotated genome sequence of the circula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Paul D,Bridges S,Burgess SC,Dandass Y,Lawrence ML

    更新日期:2008-08-01 00:00:00

  • Glucose uptake by the cellulolytic ruminal anaerobe Bacteroides succinogenes.

    abstract::Glucose uptake by Bacteroides succinogenes S85 was measured under conditions that maintained anaerobiosis and osmotic stability. Uptake was inhibited by compounds which interfere with electron transport systems, maintenance of proton or metal ion gradients, or ATP synthesis. The most potent inhibitors were proton and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Franklund CV,Glass TL

    更新日期:1987-02-01 00:00:00

  • Induction of colicin 1a at high temperature.

    abstract::Induction of colicin la at a high temperature (42 C) was demonstrated in a wild strain of Shigella sonnei. After transfer of the Col factor, a similar effect was observed in those recipient cells acquiring colicinogeny. The induction is suggested to be due to the presence of a thermosensitive colicin repressor. The wi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gromkova RH

    更新日期:1971-06-01 00:00:00

  • Novel 3,6-Dihydroxypicolinic Acid Decarboxylase-Mediated Picolinic Acid Catabolism in Alcaligenes faecalis JQ135.

    abstract::Picolinic acid (PA), a typical C-2-carboxylated pyridine derivative, is a metabolite of l-tryptophan and many other aromatic compounds in mammalian and microbial cells. Microorganisms can degrade and utilize PA for growth. However, the precise mechanism of PA metabolism remains unknown. Alcaligenes faecalis strain JQ1...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Qiu J,Zhang Y,Yao S,Ren H,Qian M,Hong Q,Lu Z,He J

    更新日期:2019-03-13 00:00:00