Specific localization of the lysis protein of bacteriophage MS2 in membrane adhesion sites of Escherichia coli.

Abstract:

:Specific localization of the lysis (L) protein of bacteriophage MS2 in the cell wall of Escherichia coli was determined by immunoelectron microscopy. After induction of the cloned lysis gene, the cells were plasmolyzed, fixed, and embedded in either Epon or Lowicryl K4M. Polyclonal L-protein-specific antiserum was purified by preabsorption to membranes from cells harboring a control plasmid. Protein A-gold was used to label the protein-antibody complexes. Between 42.8% (Lowicryl) and 33.8% (Epon) of the label was found in inner and outer membranes, but 30.3% (Lowicryl) and 32.8% (Epon) was present mostly in clusters in the adhesion sites visible after plasmolysis. The remaining label (26.9 and 33.4%, respectively) appeared to be present in the periplasmic space but may also have been part of membrane junctions not visible because of poor contrast of the specimen. In contrast, a quite different distribution of the L protein was found in cells grown under conditions of penicillin tolerance, i.e., at pH 5, a condition that had previously been shown to protect cells from L-protein-induced lysis. At tolerant conditions, only 21.0% of the L protein was in the adhesion sites; most of the protein (68.2%) was found in inner and outer membranes. It is concluded that lysis of the host, E. coli, was a result of the formation of specific L-protein-mediated membrane adhesion sites.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Walderich B,Höltje JV

doi

10.1128/jb.171.6.3331-3336.1989

subject

Has Abstract

pub_date

1989-06-01 00:00:00

pages

3331-6

issue

6

eissn

0021-9193

issn

1098-5530

journal_volume

171

pub_type

杂志文章
  • Deletion of the 389 N-terminal residues of the transcriptional activator AREA does not result in nitrogen metabolite derepression in Aspergillus nidulans.

    abstract::Utilizing a homologous gene replacement in order to retain the native promoter and 5' and 3' untranslated messenger regions (and thereby ensure physiological validity), we have shown that deletion of the N-terminal 389 amino acids of the transcriptional activator AREA does not result in nitrogen metabolite derepressio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.21.5762-5764.1998

    authors: Caddick MX,Arst HN Jr

    更新日期:1998-11-01 00:00:00

  • GerN, an antiporter homologue important in germination of Bacillus cereus endospores.

    abstract::A homologue of the grmA spore germination gene of Bacillus megaterium and of a NaH-antiporter gene (napA) of Enterococcus hirae has been identified in Bacillus cereus 569 (ATCC 10876). The putative protein product has 58 and 43% amino acid identity with GrmA and NapA, respectively. Insertional inactivation of this B. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.2.476-482.2001

    authors: Thackray PD,Behravan J,Southworth TW,Moir A

    更新日期:2001-01-01 00:00:00

  • Differences in incorporation of nucleic acid bases and nucleosides by various Mycoplasma and Acholeplasma species.

    abstract::Eight species representative of the serological diversity of the Mycoplasmatales were tested for their ability to incorporate radiolabeled nucleic acid precursors into acid-insoluble material. Cultures in complex growth medium were centrifuged and resuspended in minimal essential medium (Eagle). For Acholeplasma laidl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.2.483-489.1978

    authors: McIvor RS,Kenny GE

    更新日期:1978-08-01 00:00:00

  • Tyrosine 106 of CheY plays an important role in chemotaxis signal transduction in Escherichia coli.

    abstract::CheY is the response regulator in the signal transduction pathway of bacterial chemotaxis. Position 106 of CheY is occupied by a conserved aromatic residue (tyrosine or phenylalanine) in the response regulator superfamily. A number of substitutions at position 106 have been made and characterized by both behavioral an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.14.4208-4215.1996

    authors: Zhu X,Amsler CD,Volz K,Matsumura P

    更新日期:1996-07-01 00:00:00

  • Complete genome sequence of Thermococcus sp. strain 4557, a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent area.

    abstract::Thermococcus sp. strain 4557 is a hyperthermophilic anaerobic archaeon isolated from the deep-sea hydrothermal vent Guaymas Basin site in the Gulf of California at a depth of 2,000 m. Here, we present the complete genome sequence of Thermococcus sp. 4557, which consists of a single circular chromosome of 2,011,320 bp ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05851-11

    authors: Wang X,Gao Z,Xu X,Ruan L

    更新日期:2011-10-01 00:00:00

  • Delayed expression of in vivo restriction activity following conjugal transfer of Escherichia coli hsdK (restriction-modification) genes.

    abstract::Following conjugal transfer of the hsdK genes (hsdRK, hsdMK, and hsdSK) of Escherichia coli K-12, restriction activity was first detected only after approximately 15 generations, whereas modification activity was observed immediately. This sequential expression explains the establishment of hsdK genes in a nonmodified...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.15.4905-4906.1993

    authors: Prakash-Cheng A,Ryu J

    更新日期:1993-08-01 00:00:00

  • Periplasmic nitrate reductase (NapABC enzyme) supports anaerobic respiration by Escherichia coli K-12.

    abstract::Periplasmic nitrate reductase (NapABC enzyme) has been characterized from a variety of proteobacteria, especially Paracoccus pantotrophus. Whole-genome sequencing of Escherichia coli revealed the structural genes napFDAGHBC, which encode NapABC enzyme and associated electron transfer components. E. coli also expresses...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.5.1314-1323.2002

    authors: Stewart V,Lu Y,Darwin AJ

    更新日期:2002-03-01 00:00:00

  • Role of nitrogen regulator I (NtrC), the transcriptional activator of glnA in enteric bacteria, in reducing expression of glnA during nitrogen-limited growth.

    abstract::During nitrogen-limited growth, transcription of glnA, which codes for glutamine synthetase, requires sigma 54-RNA polymerase and the phosphorylated from the nitrogen regulator I (NRI; also called NtrC). In cells in which the lac promoter controlled expression of the gene coding for NRI, increasing the intracellular c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.1.179-185.1992

    authors: Shiau SP,Schneider BL,Gu W,Reitzer LJ

    更新日期:1992-01-01 00:00:00

  • Lon Protease Removes Excess Signal Recognition Particle Protein in Escherichia coli.

    abstract::Correct targeting of membrane proteins is essential for membrane integrity, cell physiology, and viability. Cotranslational targeting depends on the universally conserved signal recognition particle (SRP), which is a ribonucleoprotein complex comprised of the protein component Ffh and the 4.5S RNA in Escherichia coli ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00161-20

    authors: Sauerbrei B,Arends J,Schünemann D,Narberhaus F

    更新日期:2020-06-25 00:00:00

  • Effects of environmental changes on expression of the oligopeptide permease (opp) genes of Borrelia burgdorferi.

    abstract::We analyzed expression of a putative oligopeptide permease (Opp) of Borrelia burgdorferi. Unlike the opp operons of other bacteria for which there is a single substrate binding protein, B. burgdorferi codes for three substrate binding proteins (OppA-I to -III) in its opp operon and an additional two homologs on plasmi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.22.6198-6206.2002

    authors: Wang XG,Lin B,Kidder JM,Telford S,Hu LT

    更新日期:2002-11-01 00:00:00

  • THE GENUS VEILLONELLA. I. GENERAL CULTURAL, ECOLOGICAL, AND BIOCHEMICAL CONSIDERATIONS.

    abstract::Rogosa, M. (National Institutes of Health, Bethesda, Md.). The genus Veillonella. I. General cultural, ecological, and biochemical considerations. J. Bacteriol. 87:162-170. 1964.-Arguments are presented for excluding Veillonella discoides, V. reniformis, V. orbiculus, and V. vulvovaginitidis from the genus, and for re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.1.162-170.1964

    authors: ROGOSA M

    更新日期:1964-01-01 00:00:00

  • Mutations that improve export of maltose-binding protein in SecB- cells of Escherichia coli.

    abstract::It previously has been proposed that the Escherichia coli SecB protein promotes the export of the maltose-binding protein (MBP) from the cytoplasm by preventing the folding of the precursor MBP (preMBP) into a translocation-incompetent conformation. The export of wild-type MBP is only partially blocked in SecB- cells....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.4640-4647.1989

    authors: Collier DN,Bassford PJ Jr

    更新日期:1989-09-01 00:00:00

  • Isocitrate dehydrogenase kinase/phosphatase: aceK alleles that express kinase but not phosphatase activity.

    abstract::For Escherichia coli, growth on acetate requires the induction of the enzymes of the glyoxylate bypass, isocitrate lyase and malate synthase. The branch point between the glyoxylate bypass and the Krebs cycle is controlled by phosphorylation of isocitrate dehydrogenase (IDH), inhibiting that enzyme's activity and thus...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.5.1801-1806.1991

    authors: Ikeda T,LaPorte DC

    更新日期:1991-03-01 00:00:00

  • Oxidation of aliphatic olefins by toluene dioxygenase: enzyme rates and product identification.

    abstract::Toluene dioxygenase from Pseudomonas putida F1 has been studied extensively with aromatic substrates. The present work examined the toluene dioxygenase-catalyzed oxidation of various halogenated ethenes, propenes, butenes and nonhalogenated cis-2-pentene, an isomeric mix of 2-hexenes, cis-2-heptene, and cis-2-octene a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.12.3858-3865.1997

    authors: Lange CC,Wackett LP

    更新日期:1997-06-01 00:00:00

  • CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase, which is involved in the synthesis of the major wall teichoic acid in Bacillus subtilis 168, is encoded by tagF (rodC).

    abstract::Assays of CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase (CGPTase) (EC 2.7.8.12) in membranes isolated from Bacillus subtilis 168 wild type and 11 strains bearing conditional lethal thermosensitive mutations in tagB, tagD, or tagF revealed that CGPTase deficiency was associated only with mutant tagF all...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.2.646-649.1992

    authors: Pooley HM,Abellan FX,Karamata D

    更新日期:1992-01-01 00:00:00

  • Multiplicity of 3-Ketosteroid-9α-Hydroxylase enzymes in Rhodococcus rhodochrous DSM43269 for specific degradation of different classes of steroids.

    abstract::The well-known large catabolic potential of rhodococci is greatly facilitated by an impressive gene multiplicity. This study reports on the multiplicity of kshA, encoding the oxygenase component of 3-ketosteroid 9α-hydroxylase, a key enzyme in steroid catabolism. Five kshA homologues (kshA1 to kshA5) were previously i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00274-11

    authors: Petrusma M,Hessels G,Dijkhuizen L,van der Geize R

    更新日期:2011-08-01 00:00:00

  • Addition of Escherichia coli K-12 growth observation and gene essentiality data to the EcoCyc database.

    abstract::The sets of compounds that can support growth of an organism are defined by the presence of transporters and metabolic pathways that convert nutrient sources into cellular components and energy for growth. A collection of known nutrient sources can therefore serve both as an impetus for investigating new metabolic pat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01209-13

    authors: Mackie A,Paley S,Keseler IM,Shearer A,Paulsen IT,Karp PD

    更新日期:2014-03-01 00:00:00

  • Subunit interactions and protein stability in the cyanobacterial light-harvesting proteins.

    abstract::Strain 4R is a phycocyanin-minus mutant of the unicellular cyanobacterium Synechocystis sp. strain 6803. Although it lacks the light-harvesting protein phycocyanin, 4R has normal levels of phycocyanin (cpc) transcripts. Sequence analysis of the cpcB gene encoding the phycocyanin beta subunit shows an insertion mutatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.23.6798-6803.1995

    authors: Plank T,Toole C,Anderson LK

    更新日期:1995-12-01 00:00:00

  • Aeromonas salmonicida possesses two genes encoding homologs of the major outer membrane protein, OmpA.

    abstract::Two homologs of the outer membrane protein OmpA were identified in Aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. An A. salmonicida genomic DNA library was constructed by using lambda GEM-11 and recombinant phage carrying both g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.6.1623-1630.1996

    authors: Costello GM,Vipond R,MacIntyre S

    更新日期:1996-03-01 00:00:00

  • Methylation-independent and methylation-dependent chemotaxis in Rhodobacter sphaeroides and Rhodospirillum rubrum.

    abstract::In vivo and in vitro methylation, methanol production assays, and the use of specific antibodies raised against the sensory transducing protein Tar in Escherichia coli all failed to demonstrate the presence of methyl-accepting chemotaxis proteins (MCPs) in the photosynthetic bacterium Rhodobacter sphaeroides, although...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.12.5808-5814.1987

    authors: Sockett RE,Armitage JP,Evans MC

    更新日期:1987-12-01 00:00:00

  • Dependence of Escherichia coli hyperbaric oxygen toxicity on the lipid acyl chain composition.

    abstract::This study examines certain membrane-related aspects of oxygen poisoning in Escherichia coli K1060 (fabB fadE lacI) and its parent strain, K-12 Ymel. Cells were grown to exponential or stationary phase in a minimal medium and exposed to air plus 300 lb/in2 of O2 as a suspension in minimal salts. After an initial lag, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.3.808-820.1978

    authors: Harley JB,Santangelo GM,Rasmussen H,Goldfine H

    更新日期:1978-06-01 00:00:00

  • A physical map of the Salmonella typhimurium LT2 genome made by using XbaI analysis.

    abstract::XbaI digestion and pulsed-field gel electrophoresis of the genome of Salmonella typhimurium LT2 yields 24 fragments: 23 fragments (total size, 4,807 kb) are from the chromosome, and one fragment (90 kb) is from the virulence plasmid pSLT. Some of the 23 fragments from the chromosome were located on the linkage map by ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.5.1662-1672.1992

    authors: Liu SL,Sanderson KE

    更新日期:1992-03-01 00:00:00

  • Aeromonas hydrophila lateral flagellar gene transcriptional hierarchy.

    abstract::Aeromonas hydrophila AH-3 lateral flagella are not assembled when bacteria grow in liquid media; however, lateral flagellar genes are transcribed. Our results indicate that A. hydrophila lateral flagellar genes are transcribed at three levels (class I to III genes) and share some similarities with, but have many impor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01994-12

    authors: Wilhelms M,Gonzalez V,Tomás JM,Merino S

    更新日期:2013-04-01 00:00:00

  • Transcriptional analysis of the principal cell division gene, ftsZ, of Mycobacterium tuberculosis.

    abstract::Multiple promoters drive the expression of the principal cell division gene, ftsZ, in bacterial systems. Primer extension analysis of total RNA from Mycobacterium tuberculosis and a Mycobacterium smegmatis transformant containing 1.117 kb of the upstream region of M. tuberculosis ftsZ and promoter fusion studies ident...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.7.2540-2550.2005

    authors: Roy S,Ajitkumar P

    更新日期:2005-04-01 00:00:00

  • EFFECT OF AMINO ACIDS ON STEADY-STATE GROWTH OF A GROUP A HEMOLYTIC STREPTOCOCCUS.

    abstract::Davies, Helen C. (University of Pennsylvania, Philadelphia), Fred Karush, and Joanne H. Rudd. Effect of amino acids on steady-state growth of a group A hemolytic streptococcus. J. Bacteriol. 89:421-427. 1965.-A study has been made of amino acid utilization by a strain of type 4, group A streptococcus growing under ste...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Davies HC,Karush F,Rudd JH

    更新日期:1965-02-01 00:00:00

  • Acetohydroxy acid synthase activity from a mutation at ilvF in Escherichia coli K-12.

    abstract::Examination of the ilvF locus at 54 min on the Escherichia coli K-12 chromosome revealed that it is a cryptic gene for expression of a valine-resistant acetohydroxy acid synthase (acetolactate synthase; EC 4.1.3.18) distinct from previously reported isozymes. A spontaneous mutation, ilvF663, yielded IlvF+ enzyme activ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.6.3060-3065.1990

    authors: Alexander-Caudle C,Latinwo LM,Jackson JH

    更新日期:1990-06-01 00:00:00

  • Suppressor analysis reveals a role for SecY in the SecA2-dependent protein export pathway of Mycobacteria.

    abstract::All bacteria use the conserved Sec pathway to transport proteins across the cytoplasmic membrane, with the SecA ATPase playing a central role in the process. Mycobacteria are part of a small group of bacteria that have two SecA proteins: the canonical SecA (SecA1) and a second, specialized SecA (SecA2). The SecA2-depe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00630-13

    authors: Ligon LS,Rigel NW,Romanchuk A,Jones CD,Braunstein M

    更新日期:2013-10-01 00:00:00

  • Respiratory-competent conditional developmental mutant of Mucor racemosus.

    abstract::A conditional developmental mutant of Mucor racemosus which is capable of oxidative energy metabolism is described. Unlike the wild-type strain the mutant was highly fermentative and exhibited the yeast morphology when grown aerobically in glucose-containing media. The high fermentative activity and yeast morphology u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.3.1049-1056.1985

    authors: Borgia PT,Gokul NK,Phillips GJ

    更新日期:1985-12-01 00:00:00

  • Quorum sensing controls exopolysaccharide production in Sinorhizobium meliloti.

    abstract::Sinorhizobium meliloti is a soil bacterium capable of invading and establishing a symbiotic relationship with alfalfa plants. This invasion process requires the synthesis, by S. meliloti, of at least one of the two symbiotically important exopolysaccharides, succinoglycan and EPS II. We have previously shown that the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.1.325-331.2003

    authors: Marketon MM,Glenn SA,Eberhard A,González JE

    更新日期:2003-01-01 00:00:00

  • Phosphate-mediated alteration of the Microsporum gypseum germination protease specificity for substrate: enhanced keratinase activity.

    abstract::Inorganic phosphate was found to decrease the caseinolytic and ethyl-esterase activities of the Microsporum gypseum germination protease. The germination protease possessed exokeratinase (beta-keratinase) activity immediately after release from the fungal spore. After phosphate treatment of the enzyme, the germination...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.2.422-431.1974

    authors: Page WJ,Stock JJ

    更新日期:1974-02-01 00:00:00