Multiplicity of 3-Ketosteroid-9α-Hydroxylase enzymes in Rhodococcus rhodochrous DSM43269 for specific degradation of different classes of steroids.

abstract：:Genetic transformation of auxotrophs of the extreme thermophile Thermus thermophilus HB27 to prototrophy was obtained at high frequencies of 10(-2) to 10(-1) when proliferating cell populations were exposed to chromosomal DNA from a nutritionally independent wild-type strain. The transformation frequency was proportio...

journal_title：Journal of bacteriology

authors： Koyama Y,Hoshino T,Tomizuka N,Furukawa K

更新日期：1986-04-01 00:00:00

abstract：:The nonstarter lactic acid bacterium Lactococcus raffinolactis is prevalent in a wide range of environments, such as the dairy environment, but little is known about this species. Here, we present the draft genome of Lactococcus raffinolactis strain 4877, isolated from a natural mesophilic dairy starter culture. ...

journal_title：Journal of bacteriology

authors： Meslier V,Loux V,Renault P

更新日期：2012-11-01 00:00:00

abstract：:Welker, N. E. (Western Reserve University, Cleveland, Ohio), and L. Leon Campbell. Preferential synthesis of alpha-amylase by Bacillus stearothermophilus in the presence of 5-methyl-tryptophan. J. Bacteriol. 87:828-831. 1964.-Washed-cell suspensions of Bacillus stearothermophilus induced with pure maltose preferential...

journal_title：Journal of bacteriology

authors： WELKER NE,CAMPBELL LL

更新日期：1964-04-01 00:00:00

abstract：:Twelve strains of Agrobacterium radiobacter isolated from naturally occurring crown galls or soil were found to be avirulent on sunflower, tomato, Kalanchoe, and carrot. Eleven strains contained plasmids of molecular weights 77 X 10(6) to 182 X 10(6) as determined by electron microscopy. One strain contained only a sm...

journal_title：Journal of bacteriology

authors： Merlo DJ,Nester EW

更新日期：1977-01-01 00:00:00

abstract：:Cadmium-resistant Pseudomonas putida GAM-1, which was able to grow in concentrations of CdCl2 as high as 7 mM, was isolated from soil in a rice paddy. This bacterium harbored a DNA plasmid of about 52 kilobases. The plasmid (pGU100) transformed Escherichia coli C600 to cadmium resistance. A cadmium-resistant transform...

journal_title：Journal of bacteriology

authors： Horitsu H,Yamamoto K,Wachi S,Kawai K,Fukuchi A

更新日期：1986-01-01 00:00:00

abstract：:Amino acid starvation allows limited synthesis of deoxyribonucleic acid (DNA) in Bacillus subtilis strain W23. DNA synthesis increased by about 30% after leucine starvation and by about 60% after histidine starvation. Genetic analysis on the DNA synthesized after amino acid starvation showed that all genetic markers e...

journal_title：Journal of bacteriology

authors： Copeland JC

更新日期：1971-02-01 00:00:00

abstract：:Benzoyl coenzyme A (benzoyl-CoA) reductase is a key enzyme in the anaerobic metabolism of aromatic compounds catalyzing the ATP-driven reductive dearomatization of benzoyl-CoA. The enzyme from Thauera aromatica uses a reduced 2[4Fe-4S] ferredoxin as electron donor. In this work, we identified 2-oxoglutarate:ferredoxin...

journal_title：Journal of bacteriology

authors： Dörner E,Boll M

更新日期：2002-07-01 00:00:00

abstract：:Streptococcus bovis possesses two sugar phosphate phosphatases (Pases). Pase I is a soluble enzyme that is inhibited by the membrane fractions from lactose-grown cells and is insensitive to activation by S46D HPr, an analog of HPr(ser-P) of the sugar phosphotransferase system. Pase II is a membrane-associated enzyme t...

journal_title：Journal of bacteriology

authors： Cook GM,Ye JJ,Russell JB,Saier MH Jr

更新日期：1995-12-01 00:00:00

abstract：:The csgA gene produces an intercellular signal during fruiting body formation of the myxobacterium Myxococcus xanthus. Sporulating pseudorevertants were isolated to allow us to understand the mechanism by which CsgA is perceived by cells and used to regulate developmental gene expression. Two strains, LS559 and LS560,...

journal_title：Journal of bacteriology

authors： Lee K,Shimkets LJ

更新日期：1994-04-01 00:00:00

abstract：:The Bacillus subtilis PurR mediates adenine repression and guanosine induction of purA. PRPP inhibits binding of PurR to DNA in vitro. Mutations in the PRPP binding motif of PurR caused strong repression regardless of purine exclusions or additions, establishing the role of PRPP as regulator of PurR. ...

journal_title：Journal of bacteriology

authors： Rappu P,Pullinen T,Mäntsälä P

更新日期：2003-11-01 00:00:00

abstract：:The complete nucleotide sequence of pADB201, a 1.7-kilobase cryptic plasmid from Mycoplasma mycoides subsp. mycoides, is reported. The sequence contains a single large open reading frame capable of coding for a polypeptide of up to 198 codons long. The sequence of the putative polypeptide shows significant similarity ...

journal_title：Journal of bacteriology

authors： Bergemann AD,Whitley JC,Finch LR

更新日期：1989-01-01 00:00:00

abstract：:Protein 1, a major protein of the outer membrane of Escherichia coli, has been shown to be the pore allowing the passage of small hydrophilic solutes across the outer membrane. In E. coli K-12 protein 1 consists of two subspecies, 1a and 1b, whereas in E. coli B it consists of a single species which has an electrophor...

journal_title：Journal of bacteriology

authors： Pugsley AP,Schnaitman CA

更新日期：1978-03-01 00:00:00

abstract：:Binding sites for the Escherichia coli protein integration host factor (IHF) include a set of conserved bases that can be summarized by the consensus sequence WATCAANNNNTTR (W is dA or dT, R is dA or dG, and N is any nucleotide). However, additional 5'-proximal bases, whose common feature is a high dA+dT content, are ...

journal_title：Journal of bacteriology

authors： Hales LM,Gumport RI,Gardner JF

更新日期：1994-05-01 00:00:00

abstract：:Chlorate-resistant mutants corresponding to each known genetic locus (chlA, chlB, chlC, chlD, chlE) were isolated from Escherichia coli K-12. All these mutants showed decreased amounts of membrane-bound nitrate reductase, cytochrome b, and formic dehydrogenase, but all had normal succinic dehydrogenase activity. Prote...

journal_title：Journal of bacteriology

authors： MacGregor CH,Schnaitman CA

更新日期：1971-10-01 00:00:00

abstract：:The R-factor R1 is present in a low number of copies per genome (near unity, so-called stringent control of replication). The replication of R1 was studied in a density-shift experiment. One generation after the shift about 20% of the R1 copies had not replicated, whereas about 20% had replicated at least twice. The r...

journal_title：Journal of bacteriology

authors： Gustafsson P,Nordström K

更新日期：1975-08-01 00:00:00

abstract：:A 7.5-kbp fragment of chromosomal DNA downstream of the Vibrio cholerae vibriobactin outer membrane receptor, viuA, and the vibriobactin utilization gene, viuB, was recovered from a Sau3A lambda library of O395 chromosomal DNA. By analogy with the genetic organization of the Escherichia coli enterobactin gene cluster,...

journal_title：Journal of bacteriology

authors： Butterton JR,Choi MH,Watnick PI,Carroll PA,Calderwood SB

更新日期：2000-03-01 00:00:00

abstract：:SugE of Escherichia coli, first identified as a suppressor of groEL mutations but a member of the small multidrug resistance family, has not previously been shown to confer a drug resistance phenotype. We show that high-level expression of sugE leads to resistance to a subset of toxic quaternary ammonium compounds. ...

journal_title：Journal of bacteriology

authors： Chung YJ,Saier MH Jr

更新日期：2002-05-01 00:00:00

abstract：:The colicin E2 immunity (ceiB) and lysis (celB) genes of colicin plasmid ColE2-P9 were cloned as a 900-base-pair insert under the control of the lac promoter in high-copy-number plasmid pUR222. Hosts carrying this plasmid were immune to colicin E2, produced increased amounts of immunity protein (molecular weight, 9,00...

journal_title：Journal of bacteriology

authors： Pugsley AP,Schwartz M

更新日期：1983-10-01 00:00:00

abstract：:The cytoskeletal proteins HMW1 and HMW2 are components of the terminal organelle of the cell wall-less bacterium Mycoplasma pneumoniae. HMW1 is required for a tapered, filamentous morphology but exhibits accelerated turnover in the absence of HMW2. Here, we report that a reciprocal dependency exists between HMW1 and H...

journal_title：Journal of bacteriology

authors： Willby MJ,Balish MF,Ross SM,Lee KK,Jordan JL,Krause DC

更新日期：2004-12-01 00:00:00

abstract：:Escherichia coli UM146 was isolated from the ileum of a Crohn's disease patient. It adheres to and invades enterocytes and can replicate inside macrophages. Its complete genome sequence reveals that it is most closely related to the human urinary tract pathogen E. coli CFT073, but it has a host of genes that are novel...

journal_title：Journal of bacteriology

authors： Krause DO,Little AC,Dowd SE,Bernstein CN

更新日期：2011-01-01 00:00:00

abstract：:The DNA sequence of the gene which codes for the major outer membrane porin (Omp32) of Comamonas acidovorans has been determined. The structural gene encodes a precursor consisting of 351 amino acid residues with a signal peptide of 19 amino acid residues. Comparisons with amino acid sequences of outer membrane protei...

journal_title：Journal of bacteriology

authors： Gerbl-Rieger S,Peters J,Kellermann J,Lottspeich F,Baumeister W

更新日期：1991-04-01 00:00:00

abstract：:TOL plasmid pWW0 specifies enzymes for the oxidative catabolism of toluene and xylenes. The upper pathway converts the aromatic hydrocarbons to aromatic carboxylic acids via corresponding alcohols and aldehydes and involves three enzymes: xylene oxygenase, benzyl alcohol dehydrogenase, and benzaldehyde dehydrogenase. ...

journal_title：Journal of bacteriology

authors： Harayama S,Leppik RA,Rekik M,Mermod N,Lehrbach PR,Reineke W,Timmis KN

更新日期：1986-08-01 00:00:00

abstract：:The Escherichia coli pap genetic determinant includes 11 genes and encodes expression of Pap pili on the bacterial surface. An RNase E-dependent mRNA-processing event in the intercistronic papB-papA region results in the accumulation of a papA-gene-specific mRNA in considerable excess of the primary papB-papA mRNA tra...

journal_title：Journal of bacteriology

authors： Nilsson P,Naureckiene S,Uhlin BE

更新日期：1996-02-01 00:00:00

abstract：:Whole cells of Lactobacillus acidophilus are capable of degrading thymidine to thymine, suggesting the presence of thymidine phosphorylase (or thymidine hydrolase). This activity was also demonstrated in cell-free extracts. ...

journal_title：Journal of bacteriology

authors： Sawula RV,Zamenhof S,Zamenhof PJ

更新日期：1974-03-01 00:00:00

abstract：:Bacillus megaterium mutants JV-9 and JV-10 are temperature sensitive for initiation of spore germination. At 46 C, they did not lose heat resistance, dipicolinic acid, or absorbance, indicating that the temperature-sensitive blocks are very early in the sequence of initiation reactions. Strain JV-9 was temperature sen...

journal_title：Journal of bacteriology

authors： Vary JC

更新日期：1975-01-01 00:00:00

abstract：:The reactivation of membranes from Gaffkya homari for the synthesis of sodium dodecyl sulfate-insoluble peptidoglycan (SDS-insoluble PG) was achieved by successive cycles of freeze-thawing (- 196 versus 25 degrees C). The presence of G. homari walls during this process affected the synthesis of both SDS-soluble (nasce...

journal_title：Journal of bacteriology

authors： Kalomiris E,Bardin C,Neuhaus FC

更新日期：1982-05-01 00:00:00

abstract：:A modified 3-hydroxypropionate cycle has been proposed as the autotrophic CO2 fixation pathway for the thermoacidophilic crenarchaeon Metallosphaera sedula. The cycle requires the reductive conversion of 3-hydroxypropionate to propionyl-coenzyme A (propionyl-CoA). The specific activity of the 3-hydroxypropionate-, CoA...

journal_title：Journal of bacteriology

authors： Alber BE,Kung JW,Fuchs G

更新日期：2008-02-01 00:00:00

abstract：:The two 23S rRNA-5S rRNA-tRNAGly operons from the extreme thermophilic eubacterium Thermus thermophilus HB8 were used to characterized the in vivo processing and termination of 23S rRNA-5S rRNA-tRNAGly primary transcripts in this organism by nuclease S1 mapping. A processing site in the pre-23S rRNA 3'-flanking region...

journal_title：Journal of bacteriology

authors： Hartmann RK,Toschka HY,Erdmann VA

更新日期：1991-04-01 00:00:00

abstract：:The segregation of mating loci with other unselected genes was analyzed in recombinants obtained from matings of Nocardia canicruria and N. erythropolis. The loci C/c and E/e control nocardial compatibility. Four mating genotype combinations were observed: cE, Ce, CE, and ce. Strains of N. erythropolis bear the genoty...

journal_title：Journal of bacteriology

authors： Brownell GH,Kelly KL

更新日期：1969-07-01 00:00:00

abstract：:Surface protein antigens of Mycoplasma hyopneumoniae were identified by direct antibody-surface binding or by radioimmunoprecipitation of surface 125I-labeled proteins with a series of monoclonal antibodies (MAbs). Surface proteins p70, p65, p50, and p44 were shown to be integral membrane components by selective parti...

journal_title：Journal of bacteriology

authors： Wise KS,Kim MF

更新日期：1987-12-01 00:00:00