Abstract:
:The two 23S rRNA-5S rRNA-tRNAGly operons from the extreme thermophilic eubacterium Thermus thermophilus HB8 were used to characterized the in vivo processing and termination of 23S rRNA-5S rRNA-tRNAGly primary transcripts in this organism by nuclease S1 mapping. A processing site in the pre-23S rRNA 3'-flanking region is located approximately 25 nucleotides upstream of 5S rRNA and precedes a putative 23S-5S rRNA spacer antitermination box A. Cleavage at this site and 5S rRNA 5' end formation were shown to be inseparable events. Termination of transcription at the uridine cluster following the termination-associated hairpin was shown to be efficient but leaky. Subsequent to the operon, a functional promoter was detected whose -35 box coincided with the uridine-rich termination region. The promoter directed synthesis of a beta-galactosidase fusion protein in Escherichia coli.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hartmann RK,Toschka HY,Erdmann VAdoi
10.1128/jb.173.8.2681-2690.1991subject
Has Abstractpub_date
1991-04-01 00:00:00pages
2681-90issue
8eissn
0021-9193issn
1098-5530journal_volume
173pub_type
杂志文章abstract::Clinical isolates of the porcine pathogen Actinobacillus pleuropneumoniae often form adherent colonies on agar plates due to expression of an operon, pgaABCD, encoding a poly-beta-1,6-N-acetyl-D-glucosamine (PGA) extracellular matrix. The adherent colony phenotype, which correlates with the ability to form biofilms on...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01513-09
更新日期:2010-05-01 00:00:00
abstract::Lockart, Royce Z., Jr. (The University of Texas, Austin) and Barbara Horn. Interaction of an interferon with L cells. J. Bacteriol. 85:996-1002. 1963.-Data were presented on the effect of time of exposure and concentration of an interferon in provoking viral inhibition in L cells. Populations of L cells which made red...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.5.996-1002.1963
更新日期:1963-05-01 00:00:00
abstract::Bacillus subtilis strains carrying div-341 or sacU mutations, or both, have been characterized to reveal the roles of both genes in the initiation of sporulation, as well as in cell division and exoenzyme secretion. Both mutations were closely linked by transformation and caused the pleiotropic effects on sporulation ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.2.648-653.1985
更新日期:1985-08-01 00:00:00
abstract::The Rhizobium phaseoli recA gene has been cloned by interspecific complementation of the Fec phenotype of bacteriophage lambda. The cloned gene restored the recombination proficiency and conferred resistance to DNA-damaging agents (methyl methanesulfonate and nitrofurantoin) to an Escherichia coli recA mutant. The dir...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.10.3035-3040.1991
更新日期:1991-05-01 00:00:00
abstract::A series of electron micrographs showing the presence of different molecular forms representing various replication stages of plasmid deoxyribonucleic acid from Streptomyces griseus was obtained. Based upon an analysis of these electron micrographs, a tentative model for plasmid deoxyribonucleic acid replication in S....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.1.412-414.1981
更新日期:1981-04-01 00:00:00
abstract::Bacillus thuringiensis subsp. aizawai EG6346, a novel grain dust isolate, was analyzed by Southern blot hybridization for its insecticidal crystal protein (ICP) gene profile. Strain EG6346 lacks previously characterized cryIA ICP genes yet does possess novel cryI-related gene sequences. A recombinant genomic plasmid l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.13.3966-3976.1991
更新日期:1991-07-01 00:00:00
abstract::Escherichia coli possesses three distinct formate dehydrogenase enzymes encoded by the fdnGHI, fdhF, and fdoGHI operons. To examine how two of the formate dehyrogenase operons (fdnGHI and fdhF) are expressed anaerobically in the presence of low, intermediate, and high levels of nitrate, nitrite, and formate, chemostat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.17.5076-5085.2003
更新日期:2003-09-01 00:00:00
abstract:UNLABELLED:Our laboratory recently discovered that Escherichia coli cells starved for the DNA precursor dGTP are killed efficiently (dGTP starvation) in a manner similar to that described for thymineless death (TLD). Conditions for specific dGTP starvation can be achieved by depriving an E. coli optA1 gpt strain of the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00218-16
更新日期:2016-05-13 00:00:00
abstract::Azotobacter vinelandii DJ71, which contains a mutation in the nifV gene, was derepressed for nitrogenase in the presence of homocitrate. When dinitrogenase was isolated from this culture, it was found to be identical to the wild-type dinitrogenase. However, when the same NifV- strain was derepressed in the presence of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.17.5403-5405.1991
更新日期:1991-09-01 00:00:00
abstract::Defects in DNA mismatch repair (MMR) occur frequently in natural populations of pathogenic and commensal bacteria, resulting in a mutator phenotype. We identified a unique genetic element in Streptococcus pyogenes strain SF370 that controls MMR via a dynamic process of prophage excision and reintegration in response t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01569-07
更新日期:2008-10-01 00:00:00
abstract::The binding of iron-loaded human transferrin at the surface of Neisseria meningitidis is mediated by two polypeptides, Tbp1 and Tbp2. Predicted Tbp amino acid sequences from N. meningitidis strains are highly divergent. This variability is particularly pronounced throughout the Tbp2 polypeptide. In this study, a highl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.20.6207-6213.1994
更新日期:1994-10-01 00:00:00
abstract::Highly efficient promoters of coliphage T5 were identified by selecting for functional properties. Eleven such promoters belonging to all three expression classes of the phage were analyzed. Their average AT content was 75% and reached 83% in subregions of the sequences. Besides the well-known conserved sequences arou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.1.70-77.1985
更新日期:1985-10-01 00:00:00
abstract::In anaerobic cultures of Mucor rouxii, morphogenesis was strongly dependent on hexose concentration as well as pCO(2). At low levels of hexose or CO(2), or both, hyphal development occurred; at high levels, the fungus developed as yeast cells. Other dimorphic strains of Mucor responded similarly to hexose and CO(2) bu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.5.1586-1594.1968
更新日期:1968-11-01 00:00:00
abstract::Division site placement in Escherichia coli involves interactions of the MinD protein with MinC and MinE and with other MinD molecules to form membrane-associated polymeric structures. In this work, as part of a study of these interactions, we established that heterologous membrane-associated proteins such as MinD can...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.8.2993-3001.2006
更新日期:2006-04-01 00:00:00
abstract::The stable inheritance of the broad-host-range plasmid RK2 is due at least in part to functions within a region located at coordinates 32.8 to 35.9 kb, termed the RK2 par locus. This locus encodes four previously identified genes in two operons (parCBA and parD; M. Gerlitz, O. Hrabak, and H. Schwab, J. Bacteriol. 172:...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.24.8119-8132.1992
更新日期:1992-12-01 00:00:00
abstract::Both Salmonella enterica serovar Typhimurium and Escherichia coli contain the cspH gene encoding CspH, one of the cold shock proteins (CSPs). In this study, we investigated the expression of cspH in S. enterica serovar Typhimurium and found that it was induced in response to a temperature downshift during exponential ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.19.5580-5588.2001
更新日期:2001-10-01 00:00:00
abstract::Pseudomonas aeruginosa isolates from chronic lung infections often overproduce alginate, giving rise to the mucoid phenotype. Isolation of mucoid strains from chronic lung infections correlates with a poor patient outcome. The most common mutation that causes the mucoid phenotype is called mucA22 and results in a trun...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00445-20
更新日期:2020-09-23 00:00:00
abstract::Cultures of recombination-deficient strains of Escherichia coli are composed of three classes of cells: (i) viable cells, which can undergo 20 or more generations, (ii) residually dividing cells, which can undergo fewer than 20 generations (probably an average of fewer than 6), and (iii) nondividing cells, which are i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.1.242-249.1974
更新日期:1974-04-01 00:00:00
abstract::The RimM protein in Escherichia coli is associated with free 30S ribosomal subunits but not with 70S ribosomes. A DeltarimM mutant shows a sevenfold-reduced growth rate and a reduced translational efficiency, probably as a result of aberrant assembly of the ribosomal 30S subunits. The slow growth and translational def...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.20.6095-6106.2001
更新日期:2001-10-01 00:00:00
abstract:UNLABELLED:Clostridium perfringens type D strains are usually associated with diseases of livestock, and their virulence requires the production of epsilon toxin (ETX). We previously showed (J. Li, S. Sayeed, S. Robertson, J. Chen, and B. A. McClane, PLoS Pathog 7:e1002429, 2011, http://dx.doi.org/10.1371/journal.ppat....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00349-15
更新日期:2015-10-01 00:00:00
abstract::This paper describes the major transmutations of donor deoxyribonucleic acid (DNA) after uptake by competent Bacillus subtilis cells. Kinetic experiments confirm that after exposure to competent cells, donor DNA is converted to double-stranded fragments (DSF) which can be isolated as early as 30 s from the beginning o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.1.154-162.1973
更新日期:1973-10-01 00:00:00
abstract::Upon exposure to alkylating agents, Escherichia coli increases expression of aidB along with three genes (ada, alkA, and alkB) that encode DNA repair proteins. While the biological roles of the Ada, AlkA, and AlkB proteins have been defined, despite many efforts, the molecular functions of AidB remain largely unknown....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00858-10
更新日期:2010-12-01 00:00:00
abstract::TOL plasmid pWW0 specifies enzymes for the oxidative catabolism of toluene and xylenes. The upper pathway converts the aromatic hydrocarbons to aromatic carboxylic acids via corresponding alcohols and aldehydes and involves three enzymes: xylene oxygenase, benzyl alcohol dehydrogenase, and benzaldehyde dehydrogenase. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.2.455-461.1986
更新日期:1986-08-01 00:00:00
abstract::S-Adenosyl-L-methionine:precorrin-2 methyltransferase (SP2MT), which catalyzes the C-20 methylation of precorrin-2 to precorrin-3, was purified to homogeneity from extracts of a recombinant strain of Pseudomonas denitrificans derived from a cobalamin-overproducing strain. Ammonium sulfate fractionation followed by chr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.11.6245-6251.1990
更新日期:1990-11-01 00:00:00
abstract::The ToxR protein of Vibrio cholerae is an integral membrane protein that coordinately regulates the expression of virulence genes required for successful infection. ToxR has been shown to bind directly to and activate transcription of the cholera toxin (ctx) promoter. Within the amino-terminal cytoplasmic region of To...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.21.6807-6814.1992
更新日期:1992-11-01 00:00:00
abstract::When an Escherichia coli K-12 culture was starved for glucose, 50% of the cells lost viability in about 6 days. When a K-12 mutant lacking five distinct peptidase activities, CM89, was starved in the same manner, viability was lost much more rapidly; 50% of the cells lost viability in about 2 days, whereas a parent st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.3.758-763.1984
更新日期:1984-03-01 00:00:00
abstract::Filaments of the heterocyst-forming cyanobacterium Anabaena variabilis reproduced 35SO4(2)-, incorporating 35S into cysteine, methionine, glutathione, sulfolipid, and several unidentified metabolites. The majority of the incorporated label accumulated in reduced glutathione. Heterocysts isolated from labeled filaments...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.3.1067-1074.1981
更新日期:1981-06-01 00:00:00
abstract::Cupric sulfate induced mRNA specific to the copper resistance gene cluster previously cloned from Pseudomonas syringae pv. tomato PT23. mRNA from each of the four genes of this cluster responded in a similar manner to induction over time and with different concentrations of cupric sulfate. Promoter fusion constructs i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.4399-4401.1988
更新日期:1988-09-01 00:00:00
abstract::The YycFG two-component system is the only signal transduction system in Bacillus subtilis known to be essential for cell viability. This system is highly conserved in low-G+C gram-positive bacteria, regulating important processes such as cell wall homeostasis, cell membrane integrity, and cell division. Four other ge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01936-06
更新日期:2007-04-01 00:00:00
abstract::The nucleotide sequence of tnaB of the tryptophanase operon of Escherichia coli is presented. TnaB is a tryptophan-specific permease that is homologous to Mtr, a second tryptophan-specific permease, and to TyrP, a tyrosine-specific permease. Each member of this family appears to contain 11 membrane-spanning domains. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.10.3231-3234.1991
更新日期:1991-05-01 00:00:00